Experience of Application of Syngeneic Multipotent Mesenchymal Stem Cells (MMSCs) of Adipose Tissue for Treatment of Severe Radiation Skin Lesions at Various Intervals after Exposure in the Experiment

2020 ◽  
Vol 47 (11) ◽  
pp. 1552-1557
Author(s):  
Yu. B. Deshevoi ◽  
T. A. Nasonova ◽  
O. A. Dobrynina ◽  
R. V. Deev ◽  
V. G. Lebedev ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Skin Lesions ◽  
Multipotent Mesenchymal Stem Cells

scholarly journals Adipose tissue: A natural resource for multipotent mesenchymal stem cells with potential translation to trigerminal layers

2018 ◽  
Vol 51 (02) ◽  
pp. 177-181 ◽  
Author(s):  
Bhaskar Vyas ◽  
Atul Shah ◽  
Anant Marathe ◽  
Rajni Vyas ◽  
Ramesh Bhonde ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Natural Resource ◽  
Pure Culture ◽  
Science Research ◽  
Enzymatic Digestion ◽  
Translation Study ◽  
Vascular Factor ◽  
Multipotent Mesenchymal Stem Cells

ABSTRACTBackground: The article reports basic science research that establishes that adipose tissue (AT)-derived mesenchymal stem cells (MSCs) have a potential to transgerminal translation. Study Design: MSC confirmation was obtained by phenotypic spindle-shaped cells as well as with four positive and three negative markers. The translineage translation of adipose-derived MSCs (ADMSCs) was established. Materials and Methods: The lipoaspirate was subjected to enzymatic digestion with collagenase. Stromal vascular factor (SVF) was isolated. With two passages, pure culture of ADMSCs was obtained. They were translated to all the three germinal layers. Results: AT-derived SVF contains ~30% MSCs. They are capable of being translated into endoderm, mesoderm and ectoderm. Conclusion: AT is a rich source for MSCs, with immense research possibilities for regeneration and rejuvenation.

scholarly journals Anti-L-NGFR and -CD34 Monoclonal Antibodies Identify Multipotent Mesenchymal Stem Cells in Human Adipose Tissue

2010 ◽  
Vol 19 (6) ◽  
pp. 915-925 ◽  
Author(s):  
Nadia Quirici ◽  
Cinzia Scavullo ◽  
Laura de Girolamo ◽  
Silvia Lopa ◽  
Elena Arrigoni ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Monoclonal Antibodies ◽  
Human Adipose Tissue ◽  
Multipotent Mesenchymal Stem Cells

scholarly journals Osteogenic Differentiation of Human Adipose Tissue-Derived MSCs by Non-Toxic Calcium Poly(ethylene phosphate)s

2019 ◽  
Vol 20 (24) ◽  
pp. 6242 ◽  
Author(s):  
Ilya Nifant’ev ◽  
Tatiana Bukharova ◽  
Alexander Dyakonov ◽  
Dmitry Goldshtein ◽  
Elena Galitsyna ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Osteogenic Differentiation ◽  
Maxillofacial Surgery ◽  
Human Adipose Tissue ◽  
Bone Morphogenetic Protein 2 ◽  
Gene Marker ◽  
Multipotent Mesenchymal Stem Cells ◽  
Poly Ethylene

There is a current clinical need for the development of bone void fillers and bioactive bone graft substitutes. The use of mesenchymal stem cells (MSCs) that are seeded into 3D scaffolds and induce bone generation in the event of MSCs osteogenic differentiation is highly promising. Since calcium ions and phosphates promote the osteogenic differentiation of MSCs, the use of the calcium complexes of phosphate-containing polymers is highly prospective in the development of osteogenic scaffolds. Calcium poly(ethylene phosphate)s (PEP-Ca) appear to be potentially suitable candidates primarily because of PEP’s biodegradability. In a series of experiments with human adipose-tissue-derived multipotent mesenchymal stem cells (ADSCs), we demonstrated that PEP-Ca are non-toxic and give rise to osteogenesis gene marker, bone morphogenetic protein 2 (BMP-2) and mineralization of the intercellular matrix. Owing to the synthetic availability of poly(ethylene phosphoric acid) block copolymers, these results hold out the possibility for the development of promising new polymer composites for orthopaedic and maxillofacial surgery.

scholarly journals Characterization of multipotent mesenchymal stem cells isolated from adipose tissue and bone marrow in pigs

2013 ◽  
Vol 53 (1) ◽  
pp. 37-42
Author(s):  
Ah-Young Lee ◽  
Gyeong-Im Choe ◽  
Jin-Ju Nah ◽  
ByungJae So ◽  
Kyung-Woo Lee ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Multipotent Mesenchymal Stem Cells

scholarly journals Directed differentiation of stem cells that are isolated from the adipose tissue

2013 ◽  
Vol 17 (1 (65)) ◽  
pp. 92-95
Author(s):  
Yu. V. Poliachenko ◽  
E. M. Zapol’s’ka ◽  
R. V. Saliutin
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Experimental Study ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Mesenchymal Cells ◽  
Directed Differentiation ◽  
Alternative Source ◽  
Specific Differentiation ◽  
Multipotent Mesenchymal Stem Cells

The authors have carried out an experimental study for the purpose of determining a possibility of differentiating stem cells that are isolated from the adipose tissue according to the adipogenic orientation. The results of the research were indicative of the fact that the cells isolated from the adipose tissue are of specific differentiation, namely, an osteogenic, chondrogenic and, above all, adipogenic orientation that is an evidence of the multipotency of the stem mesenchymal cells of the adipose tissue. The adipose tissue is an alternative source to the bone marrow of multipotent mesenchymal stem cells that can be used as a protection of transplanted autologous adipose tissue from resorption by lipofilling.

scholarly journals Comparative analysis of mouse bone marrow and adipose tissue mesenchymal stem cells for critical limb ischemia cell therapy

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Pegah Nammian ◽  
Seyedeh-Leili Asadi-Yousefabad ◽  
Sajad Daneshi ◽  
Mohammad Hasan Sheikhha ◽  
Seyed Mohammad Bagher Tabei ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Cell Therapy ◽  
Femoral Artery ◽  
Critical Limb Ischemia ◽  
Limb Ischemia

Abstract Introduction Critical limb ischemia (CLI) is the most advanced form of peripheral arterial disease (PAD) characterized by ischemic rest pain and non-healing ulcers. Currently, the standard therapy for CLI is the surgical reconstruction and endovascular therapy or limb amputation for patients with no treatment options. Neovasculogenesis induced by mesenchymal stem cells (MSCs) therapy is a promising approach to improve CLI. Owing to their angiogenic and immunomodulatory potential, MSCs are perfect candidates for the treatment of CLI. The purpose of this study was to determine and compare the in vitro and in vivo effects of allogeneic bone marrow mesenchymal stem cells (BM-MSCs) and adipose tissue mesenchymal stem cells (AT-MSCs) on CLI treatment. Methods For the first step, BM-MSCs and AT-MSCs were isolated and characterized for the characteristic MSC phenotypes. Then, femoral artery ligation and total excision of the femoral artery were performed on C57BL/6 mice to create a CLI model. The cells were evaluated for their in vitro and in vivo biological characteristics for CLI cell therapy. In order to determine these characteristics, the following tests were performed: morphology, flow cytometry, differentiation to osteocyte and adipocyte, wound healing assay, and behavioral tests including Tarlov, Ischemia, Modified ischemia, Function and the grade of limb necrosis scores, donor cell survival assay, and histological analysis. Results Our cellular and functional tests indicated that during 28 days after cell transplantation, BM-MSCs had a great effect on endothelial cell migration, muscle restructure, functional improvements, and neovascularization in ischemic tissues compared with AT-MSCs and control groups. Conclusions Allogeneic BM-MSC transplantation resulted in a more effective recovery from critical limb ischemia compared to AT-MSCs transplantation. In fact, BM-MSC transplantation could be considered as a promising therapy for diseases with insufficient angiogenesis including hindlimb ischemia.

scholarly journals Extracellular Vesicles Do Not Mediate the Anti-Inflammatory Actions of Mouse-Derived Adipose Tissue Mesenchymal Stem Cells Secretome

2021 ◽  
Vol 22 (3) ◽  
pp. 1375
Author(s):  
María Carmen Carceller ◽  
María Isabel Guillén ◽  
María Luisa Gil ◽  
María José Alcaraz
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Extracellular Vesicles ◽  
Nuclear Factor Κb ◽  
Peritoneal Macrophages ◽  
Toll Like Receptor 4 ◽  
Anti Inflammatory ◽  
Phagocytic Response

Adipose tissue represents an abundant source of mesenchymal stem cells (MSC) for therapeutic purposes. Previous studies have demonstrated the anti-inflammatory potential of adipose tissue-derived MSC (ASC). Extracellular vesicles (EV) present in the conditioned medium (CM) have been shown to mediate the cytoprotective effects of human ASC secretome. Nevertheless, the role of EV in the anti-inflammatory effects of mouse-derived ASC is not known. The current study has investigated the influence of mouse-derived ASC CM and its fractions on the response of mouse-derived peritoneal macrophages against lipopolysaccharide (LPS). CM and its soluble fraction reduced the release of pro-inflammatory cytokines, adenosine triphosphate and nitric oxide in stimulated cells. They also enhanced the migration of neutrophils or monocytes, in the absence or presence of LPS, respectively, which is likely related to the presence of chemokines, and reduced the phagocytic response. The anti-inflammatory effect of CM may be dependent on the regulation of toll-like receptor 4 expression and nuclear factor-κB activation. Our results demonstrate the anti-inflammatory effects of mouse-derived ASC secretome in mouse-derived peritoneal macrophages stimulated with LPS and show that they are not mediated by EV.

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