Blood supply of the anal canal in patients with chronic hemorrhoids

The most important factors in the development of chronic haemorrhoids today are considered to be the combination of two factors (vascular and mechanical) that lead to the development of hemorrhoids. The underlying vascular factor is the vascular dysfunction, providing arterial blood flow through the arteries to the cavernous bodies and outflow through the cavernous veins, which leads to dilation of cavernous bodies and the formation of vascular malformations.There were performed clinical examination and treatment of 140 patients with chronic hemorrhoids of stage III-IV according to Goligher. The features of arterial blood supply of the anal canal were evaluated by transrectal ultrasound examination.It was found that there was no clear linear relationship between the number of anal arteries with increased blood flow and the number of hemorrhoidal nodes in the patient. Each node was supplied with blood from one or two arteries: the node placed at 11 o'clock had blood supply from the arteries visualized at 10 and 11 o'clock, the node at 3 o'clock - arteries at 3 and 5 o'clock, the node at 7 o'clock - arteries at 7 and 9 o'clock. The arteries were most frequently visualized at the first (89.4%), the third (93.3%), the seventh (88.8%) and the eleventh (93.4%) hours. With less frequency the hemodynamically significant arteries were visualized at the fifth (65.0%), the ninth (62.8%) and the tenth (66.7%) hours. The arteries that were suppliing blood hemorrhoidal vessels were located in the internal sphincter at a depth of 5 to 10 mm. In the area of 3, 7 and 11 hours, they overlapped with a mosaic pattern that corresponded to the localization of the cavernous body and resembled an arteriovenous fistula according to the СDS.

Morphometric features of blood vessels in adenomas and carcinomas of the parathyroid gland

In this article discussed the problem of neoangiogenesis in neoplastic processes of the parathyroid gland. Applying morphometric methods for the assessment of sectional tissue samples of patients with adenoma and carcinoma of the parathyroid gland, we obtained the results of quantitative and qualitative vascular growth during neoangiogenesis. At present, there are not many studies that assess vascular factor in parathyroid cancer. This study may be useful in research cancer treatment, as it highlights the pathological aspects of the formation of new blood vessels, which is similar in all malignant processes.

Analysis of the vascular abnormalities of the patients with ankle joint mild osteoarthritis

The vascular factor is one of the leading pathogenesis factors in the formation of ankle joint osteoarthritis. Dystrophic and sclerotic changes in the joint tissues develop as a result of blood flow decrease. These mechanisms understanding will allow to plan treatment and rehabilitation measures, as well as predict and prevent complications. The purpose of the work is to study hemodynamic parameters in the main lower leg arteries of the in patients in follow-up period of mild ankle joint osteoarthritis. Two groups of patients were examined. The first group - 82 patients with mild ankle joint osteoarthritis in the follow-up period (10 years) and the second group - control (healthy) group of 58 people without ankle joint osteoarthritis. Duplex scan of the main lower leg arteries was performed to all the patients. The state of arteries and hemodynamic parameters were evaluated. Excell and STATISTICA 10.0 programs were used for statistical data processing. In patients with follow-up of mild ankle joint osteoarthritis, the diameter of the arteries did not differ from the control group. In patients with mild ankle joint osteoarthritis the thickness of the Intima-media complex in the lower leg arteries and walls pulsation were significantly higher than those in patients of the control group (p <0.05). Analysis of hemodynamic parameters in patients with ankle joint osteoarthritis revealed an increase in the linear velocity of blood flow with a further tendency to normalization and even decrease in the follow-up compared with the control group. Signs of perfusion difficulty that accompanied the development of high blood pressure syndrome in the lower leg arteries were observed in 122 (67.0%) patients, and the signs of perfusion difficulty were bilateral in most of the cases (86.9%). Stenosis, deformation and arteries tortuosity were noted in 22% of patients with ankle joint osteoarthritis. Thus, mild ankle joint osteoarthritis is accompanied by blood flow changes in the form of inadequate perfusion and high-pressure syndrome in the lower leg arteries, which can cause secondary injuries and requires higher attention when selecting treatment and rehabilitation actions.

Bioinspired inorganic nanoparticles and vascular factor microenvironment directed neo-bone formation

VEGF-overexpressing human tonsil-derived stem cells (VEGF-hTMSCs) and a whitlockite microenvironment induce vascularized bone tissue formation in biodegradable polymeric scaffolds.

ПОРІВНЯЛЬНІ ВІДМІННОСТІ ПРОСТОРОВОЇ РЕОРГАНІЗАЦІЇ ПЛАЦЕНТ У ЖІНОК ІЗ СИНДРОМОМ ВТРАТИ ПЛОДА БЕЗ КОРЕКЦІЇ ТА НА ФОНІ МЕДИКАМЕНТОЗНОГО ЛІКУВАННЯ

Fetal loss syndrome is a polyethyologic syndrome. In the genesis of reproductive losses, great importance is given to the inferior transformation of the endometrium and to the disturbance of trophoblast invasion processes. Study of changes in the placenta inherent in the fetal loss syndrome will allow to understand the pathogenesis of syndrome formation, to reveal the role of the vascular factor in the development of degenerative changes and to choose pathogenetically valid correction of the revealed disorders.The aim of the study – to compare the macro and microscopic structure of the placenta of women with fetal loss syndrome without additional correction and against the background of the proposed medical treatment.Materials and Methods. 100 placenta examination (group I formed 50 placenta of women who received medical therapy were performed, provided by the provisions of the clinical protocols of the Ministry of Health of Ukraine, group II – placenta mothers, who during the pregnancy additionally appointed a donor of nitric oxide and the drug of vitamin B9).Results and Discussion. In the visual assessment and investigation of the histological structure of the placenta of the I group, we have been detected various pathological changes that are characteristic of the dystrophic-degenerative processes. In the second group placenta is noted reduction of pathological manifestations and activation of adaptive-compensatory processes.Conclusions. Integrated therapy using a nitric oxide dopant and vitamin B9 helps to improve metabolic processes in the fetoplacental system.

Adipose tissue: A natural resource for multipotent mesenchymal stem cells with potential translation to trigerminal layers

ABSTRACTBackground: The article reports basic science research that establishes that adipose tissue (AT)-derived mesenchymal stem cells (MSCs) have a potential to transgerminal translation. Study Design: MSC confirmation was obtained by phenotypic spindle-shaped cells as well as with four positive and three negative markers. The translineage translation of adipose-derived MSCs (ADMSCs) was established. Materials and Methods: The lipoaspirate was subjected to enzymatic digestion with collagenase. Stromal vascular factor (SVF) was isolated. With two passages, pure culture of ADMSCs was obtained. They were translated to all the three germinal layers. Results: AT-derived SVF contains ~30% MSCs. They are capable of being translated into endoderm, mesoderm and ectoderm. Conclusion: AT is a rich source for MSCs, with immense research possibilities for regeneration and rejuvenation.