A new field method for measuring forest litter respiration rate

A number of satisfactory methods are available for the electron microscopy of nicleic acids. These methods concentrated on fragments of nuclear, viral and mitochondrial DNA less than 50 megadaltons, on denaturation and heteroduplex mapping (Davies et al 1971) or on the interaction between proteins and DNA (Brack and Delain 1975). Less attention has been paid to the experimental criteria necessary for spreading and visualisation by dark field electron microscopy of large intact issociations of DNA. This communication will report on those criteria in relation to the ultrastructure of the (approx. 1 x 10-14g) DNA component of the kinetoplast from Trypanosomes. An extraction method has been developed to eliminate native endonucleases and nuclear contamination and to isolate the kinetoplast DNA (KDNA) as a compact network of high molecular weight. In collaboration with Dr. Ch. Brack (Basel [nstitute of Immunology), we studied the conditions necessary to prepare this KDNA Tor dark field electron microscopy using the microdrop spreading technique.

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A Many-Beam Technique for Enhanced Resolution of Partial Dislocations

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100096485 ◽

1972 ◽

Vol 30 ◽

pp. 646-647

Author(s):

J. M. Oblak ◽

B. H. Kear

Keyword(s):

Phase Shift ◽

Field Method ◽

Dark Field ◽

Bright Field ◽

Field Technique ◽

Weak Beam ◽

Partial Dislocations ◽

Enhanced Resolution ◽

Nickel Base ◽

Beam Technique

The “weak-beam” and systematic many-beam techniques are the currently available methods for resolution of closely spaced dislocations or other inhomogeneities imaged through strain contrast. The former is a dark field technique and image intensities are usually very weak. The latter is a bright field technique, but generally use of a high voltage instrument is required. In what follows a bright field method for obtaining enhanced resolution of partial dislocations at 100 KV accelerating potential will be described.A brief discussion of an application will first be given. A study of intermediate temperature creep processes in commercial nickel-base alloys strengthened by the Ll2 Ni3 Al γ precipitate has suggested that partial dislocations such as those labelled 1 and 2 in Fig. 1(a) are in reality composed of two closely spaced a/6 <112> Shockley partials. Stacking fault contrast, when present, tends to obscure resolution of the partials; thus, conditions for resolution must be chosen such that the phase shift at the fault is 0 or a multiple of 2π.

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Low Temperature and Breaking of Dormancy Effects on Respiration Rate, Sugars, Phenolics and Peroxidase Activity Changes in Inner Buds of Onion Allium cepa L.

Acta Agriculturae Scandinavica Section B - Soil & Plant Science ◽

10.1080/09064710310003358 ◽

2003 ◽

Vol 53 (1) ◽

pp. 16-20 ◽

Cited By ~ 2

Author(s):

N. Benkeblia

Keyword(s):

Low Temperature ◽

Respiration Rate ◽

Allium Cepa ◽

Peroxidase Activity ◽

Allium Cepa L

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The effect of handling on the yield of two populations of Lolium perenne selected for differences in mature leaf respiration rate

Physiologia Plantarum ◽

10.1034/j.1399-3054.1993.890215.x ◽

1993 ◽

Vol 89 (2) ◽

pp. 341-346 ◽

Cited By ~ 1

Author(s):

Edwin Kraus ◽

Hans Lambers ◽

Chris Kolloffel

Keyword(s):

Respiration Rate ◽

Lolium Perenne ◽

Mature Leaf ◽

Leaf Respiration ◽

Two Populations

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CRITICAL PROPERTIES OF T. G. S. AND R. S. AS DETERMINED BY a d. c. ELECTRIC FIELD METHOD

Le Journal de Physique Colloques ◽

10.1051/jphyscol:1972281 ◽

1972 ◽

Vol 33 (C2) ◽

pp. C2-235-C2-236

Author(s):

A. LEVSTIK ◽

M. BURGAR ◽

R. BLINC

Keyword(s):

Electric Field ◽

Field Method ◽

Critical Properties ◽

Electric Field Method

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EFFECT OF VARIOUS HORMONES ON THE KIDNEY RESPIRATION RATES IN THE CUTTHROAT TROUT (SALMO CLARKI CLARKI)

Acta Endocrinologica ◽

10.1530/acta.0.xxxiii0428 ◽

1960 ◽

Vol XXXIII (III) ◽

pp. 428-436 ◽

Cited By ~ 4

Author(s):

W. N. Holmes

Keyword(s):

Single Dose ◽

Respiration Rate ◽

Cutthroat Trout ◽

Adaptive Mechanism ◽

Respiration Rates ◽

Salmo Clarki

ABSTRACT Relatively large doses of vasopressin administered intraperitoneally to the trout significantly enhanced the kidney respiration rate. In contrast to vasopressin a single dose of oxytocin depressed the kidney Qo2 value. This depression continued throughout the observed 24 hour period after injection. Cortisol enhanced the kidney Qo2 values significantly and to a greater extent than vasopressin. These results are discussed in relation to possible adaptive mechanism in euryhaline species of teleosts.

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