Kinetic and thermodynamic analysis of dimerization inhibitors binding to HIV protease monomers by surface plasmon resonance

2012 ◽  
Vol 6 (1) ◽  
pp. 94-97 ◽  
Author(s):  
P. V. Ershov ◽  
O. V. Gnedenko ◽  
A. A. Molnar ◽  
A. V. Lisitsa ◽  
A. S. Ivanov ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Thermodynamic Analysis ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Hiv Protease ◽  
Kinetic And Thermodynamic Analysis ◽  
Dimerization Inhibitors

scholarly journals Kinetic and Thermodynamic Analysis of Dimerization Inhibitors Binding to HIV Protease Monomers by Surface Plasmon Resonance

2012 ◽  
Vol 58 (1) ◽  
pp. 43-49 ◽  
Author(s):  
P.V. Ershov ◽  
O.V. Gnedenko ◽  
A.A. Molnar ◽  
A.V. Lisitsa ◽  
A.S. Ivanov ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Complex Formation ◽  
Thermodynamic Parameters ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Hiv Protease ◽  
Kinetic And Thermodynamic Parameters ◽  
Kinetic And Thermodynamic Analysis ◽  
Kinetic Equilibrium ◽  
Dimerization Inhibitors

Here, we describe the analysis of kinetic and thermodynamic parameters for binding of peptide and nonpeptide dimerization inhibitors to immobilized HIV protease (HIVp) monomers by using surface plasmon resonance. Molecular interactions were investigated at different inhibitors concentrations (0-80 μM) and temperatures (15-35°C). The kinetic, equilibrium and thermodynamic parameters have been determined. It was found that both inhibitors were characterized by similar interaction parameters. The complex formation is entropically driven process for both inhibitors. The entropic term(-ТΔS) had the value about -20 kcal/mol while the enthalpic term (ΔH) had the positive value about 14 kcal/mol and counteracted the complex formation.

scholarly journals Surface plasmon resonance and molecular docking studies of bovine serum albumin interaction with neomycin: kinetic and thermodynamic analysis

Bioimpacts ◽  
2017 ◽  
Vol 7 (2) ◽  
pp. 91-97 ◽  
Author(s):  
Maryam Sharifi ◽  
Jafar Ezzati Nazhad Dolatabadi ◽  
Farzaneh Fathi ◽  
Mostafa Zakariazadeh ◽  
Abolfazl Barzegar ◽  
...  
Keyword(s):  
Molecular Docking ◽  
Surface Plasmon Resonance ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Thermodynamic Analysis ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Bovine Serum ◽  
Docking Studies ◽  
Kinetic And Thermodynamic Analysis

Comparison of sensitivity of the refractometric methods of frustrated total internal reflection and surface plasmon resonance

2020 ◽  
pp. 44-49
Author(s):  
I. N. Pavlov
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Total Internal Reflection ◽  
Resonance Method ◽  
Internal Reflection ◽  
Optical Methods ◽  
Thin Boundary Layer ◽  
Experimental Conditions ◽  
Frustrated Total Internal Reflection

Two optical methods, namely surface plasmon resonance imaging and frustrated total internal reflection, are described in the paper in terms of comparing their sensitivity to change of refractive index of a thin boundary layer of an investigated medium. It is shown that, despite the fact that the theoretically calculated sensitivity is higher for the frustrated total internal reflection method, and the fact that usually in practice the surface plasmon resonance method, on the contrary, is considered more sensitive, under the same experimental conditions both methods show a similar result.

Surface Plasmon Resonance Based Sensitive Immunosensor for Benzaldehyde Detection

2010 ◽  
Vol 130 (7) ◽  
pp. 269-274 ◽  
Author(s):  
Takeshi Onodera ◽  
Takuzo Shimizu ◽  
Norio Miura ◽  
Kiyoshi Matsumoto ◽  
Kiyoshi Toko
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance
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