The use of a novel reagent, Cu-FL, for determination of NO synthesis stimulated by the medicinal leech salivary cell secretion in human endothelial cell (HUVEC) and rat cardiomyocyte cultures

2013 ◽  
Vol 7 (1) ◽  
pp. 16-26
Author(s):  
I. P. Baskova ◽  
A. Yu. Alekseeva ◽  
S. V. Kostyuk ◽  
M. E. Neverova ◽  
T. D. Smirnova ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Medicinal Leech ◽  
Human Endothelial Cell ◽  
Cell Secretion ◽  
Salivary Cell

scholarly journals Use of the most recent reagent (CuF) for stimulation of NO synthesis by the medicinal leech salivary cell secretion in the cultures of human endothelium cells (HUVEC) and in rat cardiomiocytes

2012 ◽  
Vol 58 (1) ◽  
pp. 65-76 ◽  
Author(s):  
I.P. Baskova ◽  
A.Yu. Alekseeva ◽  
S.V. Kostyuk ◽  
M.E. Neverova ◽  
T.D. Smirnova ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Culture Fluid ◽  
Medicinal Leech ◽  
No Production ◽  
Cell Secretion ◽  
Space Localization ◽  
Endothelium Cells ◽  
Salivary Cell ◽  
Human Endothelium ◽  
Specific Reagent

The medicinal leech salivary cell secretion (SCS) may stimulate NO-production in cultures of human endothelium cells (HUVEC) and rat cardiomiocytes (RCM). This effect was detected using a NO specific reagent, - the complex Cu2+ with a fluorescein derivative (Cu-Fl). NO had also been detected in the cells by fluorescent electronic microscopy and determined quantitatively in the cells and in culture fluid by the fluorescence method. SCS stimulated NO synthesis in HUVEC cells (but not in RCM) is accompanied by NO release into intercellular space. Localization of NO synthesis сenters is presented and it is shown that the increase in NO levels during the SCS action on HUVEC and RCM is associated with the increase in the activity of eNOS/nNOS, but not iNOS. In endothelial cells SCS activates nitrosylation processes, assessed by the increase of nitrite-ions in the culture medium. It is therefore important to use Cu-Fl, other than Griss-reagent, during the first hour of analysis of NO synthesis. The NO-depended mechanism of SCS action on endothelial cells might be a factor in providing of its positive action in hirudotheraphy.

scholarly journals Kaempferol alleviates human endothelial cell injury through circNOL12/miR-6873-3p/FRS2 axis

2021 ◽  
Vol 137 ◽  
pp. 111419
Author(s):  
Shuangzhan Li ◽  
Meihua Hao ◽  
Taisheng Wu ◽  
Zixuan Wang ◽  
Xicheng Wang ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Cell Injury ◽  
Human Endothelial Cell ◽  
Endothelial Cell Injury

scholarly journals ESM-1 Is a Novel Human Endothelial Cell-specific Molecule Expressed in Lung and Regulated by Cytokines

1996 ◽  
Vol 271 (34) ◽  
pp. 20458-20464 ◽  
Author(s):  
Philippe Lassalle ◽  
Sophie Molet ◽  
Anne Janin ◽  
José Van der Heyden ◽  
Jan Tavernier ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Human Endothelial Cell

Agonist Stimulated Changes in Human Endothelial Cell Cytosolic Calcium

1989 ◽  
pp. 165-172
Author(s):  
Trevor J. Hallam ◽  
J. E. Merritt ◽  
T. J. Rink ◽  
R. Jacob
Keyword(s):  
Endothelial Cell ◽  
Cytosolic Calcium ◽  
Human Endothelial Cell
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