Estimation of exotoxin production by different strains of Bacillus thuringiensis using 32P-labelled exotoxin

1973 ◽  
Vol 38 (1) ◽  
pp. 298-303 ◽  
Author(s):  
K. Šebesta ◽  
K. Horská ◽  
J. Vaňková
Keyword(s):  
Bacillus Thuringiensis ◽  
Different Strains

Rumen degradability and gas production as influenced by different strains of Bacillus thuringiensis

2019 ◽  
Vol 99 (4) ◽  
pp. 951-954
Author(s):  
F.C. Campos ◽  
A.L. Abdalla Filho ◽  
P.S. Corrêa ◽  
C. Nazato ◽  
R.G. Monnerat ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Dry Matter ◽  
Gas Production ◽  
Fibrobacter Succinogenes ◽  
Production Technique ◽  
Fermentation Processes ◽  
Ruminal Microorganisms ◽  
In Vitro Gas Production ◽  
Different Strains

The effect of six Bacillus thuringiensis (Bt) strains on diet degradability was evaluated using an in vitro gas production technique. Spores (5.7 × 106 spores) of different Bt strains (907, 1192, 2036, 2493, 2496, and S1185) plus a control (no spores) were used as treatments with four replicates (inocula) in duplicate. Fermentation processes were evaluated and ruminal microorganisms were quantified. Compared with the control, the Bt907 strain decreased dry matter (DM) and organic matter (OM) degradability without affecting the Fibrobacter succinogenes population, whereas the other strains reduced this population without altering DM and OM degradability.

scholarly journals Plasmid Patterns of Bacillus thuringiensis Type Strains

2007 ◽  
Vol 74 (1) ◽  
pp. 125-129 ◽  
Author(s):  
Arturo Reyes-Ramírez ◽  
Jorge E. Ibarra
Keyword(s):  
Bacillus Thuringiensis ◽  
Gel Electrophoresis ◽  
Extrachromosomal Dna ◽  
Basic Pattern ◽  
Agarose Gels ◽  
Strain Characterization ◽  
Different Strains ◽  
Characterization Procedure ◽  
Unique Plasmid ◽  
Type Strains

ABSTRACT Practically all Bacillus thuringiensis strains contain a set of self-replicating, extrachromosomal DNA molecules or plasmids, which vary in number and size in the different strains. The plasmid patterns obtained from gel electrophoresis have previously been used as a tool to characterize strains, but comparison of the plasmid patterns has been limited in the number and diversity of strains analyzed. In this report, we were able to compare the plasmid patterns of 83 type strains (out of 84) and 47 additional strains from six serotypes. The information obtained from this comparison showed the importance of this tool as a strain characterization procedure and indicates the complexity and uniqueness of this feature. For example, with one exception, all type strains showed a unique plasmid pattern. All were unique in such a way that none showed even a single comigrating plasmid in the agarose gels, and therefore, cluster analysis was impossible, indicating that plasmid patterns are qualitative rather than quantitative features. Furthermore, comparison between strains belonging to the same serotype showed a great difference in variability. Some serotypes (e.g., israelensis) showed the same basic pattern among all its strains, while other serotypes (e.g., morrisoni) showed a great diversity of patterns. These results indicate that plasmid patterns are valuable tools to discriminate strains below the serotype level.

scholarly journals Dynamic Model for Biomass and Proteins Production by Three Bacillus Thuringiensis ssp Kurstaki Strains

Processes ◽  
2021 ◽  
Vol 9 (12) ◽  
pp. 2147
Author(s):  
Tatiana Segura Monroy ◽  
Nouha Abdelmalek ◽  
Souad Rouis ◽  
Mireille Kallassy ◽  
Jihane Saad ◽  
...  
Keyword(s):  
Experimental Data ◽  
Bacillus Thuringiensis ◽  
Dynamic Model ◽  
Akaike Information Criterion ◽  
Total Protein ◽  
Kinetic Models ◽  
Information Criterion ◽  
Growth Limitation ◽  
Protein Productivity ◽  
Different Strains

Bacillus thuringiensis is a microorganism used for the production of biopesticides worldwide. In the present paper, different kinetic models were analyzed to study and compare three different strains of Bt ssp kurstaki (LIP, BLB1, and HD1). Bioperformances (vegetative cell, spore, substrate, and protein) and successive culture phases (oxidative growth, limitation and sporulation, and protein release) were depicted with an overarching aim to estimate total protein productivity, yield, and titer. In the end, two models were calibrated using experimental dataset (11 batches culture in 3 L bioreactor with semisynthetic medium), subsequently validated, and statistically compared. Both models satisfactorily followed the dynamics of the experimental data. Finally, a dynamic model was selected following the Akaike information criterion (AIC).

scholarly journals Isolation, characterization and toxicity of native Bacillus thuringiensis isolates from different hosts and habitats in Iran

2017 ◽  
Vol 57 (3) ◽  
pp. 212-218
Author(s):  
Akbar Ghassemi-Kahrizeh ◽  
Ayda Khoramnezhad ◽  
Reza Talaei-Hassanloui
Keyword(s):  
Bacillus Thuringiensis ◽  
Larval Mortality ◽  
Diamondback Moth ◽  
Microbial Control ◽  
Sds Page ◽  
Molecular Masses ◽  
Sterile Plastic ◽  
Different Strains ◽  
Native Strains ◽  
Plastic Containers

AbstractBacillus thuringiensisis a Gram-positive, aerobic, facultative anaerobic and endospore-forming bacterium. Different strains of this species have the ability to produce parasporal crystalline inclusions which are toxic to larvae of different insect orders and other invertebrates and cause rapid death of the host. To determine the importance of this species in microbial control, we collected native strains and studied their virulence on the diamondback moth,Plutella xylostella. More than 148 samples were collected from Alborz, Guilan and Mazandaran Provinces. Experimental samples, including soil samples from forests, fruit gardens, agricultural fields, diseased and dead larvae, were transferred to a laboratory in sterile plastic containers. For evaluatingB. thuringiensisisolates virulence, a cabbage leaf dip method with 106cell · ml−1concentration of various Bt isolates was applied to diamondback moths. Larval mortality was recorded 72 h after treatment. Based on bioassay results, all isolates were classified into three high, medium and low virulence groups. Protein level characterization based on the SDS-PAGE gel analysis showed that two isolates from a high virulence group have proteins of high molecular masses of 121 and 109 kDa. Results revealed that there is a positive correlation between protein masses and virulence of isolates. In addition, this research introduced nine strains that are highly toxic toP. xylostellaand would be valuable as insecticidal agents for controlling lepidopteran pests.

scholarly journals Morphological Study of Bacillus thuringiensis Crystals and Spores

2020 ◽  
Vol 11 (1) ◽  
pp. 155
Author(s):  
Hadi Loutfi ◽  
Nancy Fayad ◽  
Fabrice Pellen ◽  
Bernard Le Jeune ◽  
Maissa Chakroun ◽  
...  
Keyword(s):  
Electron Microscope ◽  
Statistical Analysis ◽  
Bacillus Thuringiensis ◽  
Morphological Study ◽  
Agar Medium ◽  
Morphological Data ◽  
Aspect Ratios ◽  
Different Shapes ◽  
Different Strains ◽  
Scanning Electron

In this paper, we report a morphological study of the crystals and spores of different shapes synthesized by seven different strains of Bacillus thuringiensis. Crystals and spores were separated after 48 h of culture on T3 agar medium and imaged under a scanning electron microscope (SEM). Sizes of the crystals and spores were determined using Image J software. The results showed that crystal and spore sizes were normally distributed. In addition, the volumes and aspect ratios of the crystals and spores were calculated. The statistical analysis of the data showed the variability of the size distribution and morphological data of the crystals produced by the analyzed strains. Furthermore, variations in spore size and shape within the same serovar were observed, indicating that, perhaps, there are still some unexplored differences between strains of this serovar, making them less identical than what was believed so far.

Ultrastructural Changes of the Symbiotic Chlorella-Like Alga Isolated from Hydra Viridis

1982 ◽  
Vol 40 ◽  
pp. 320-321
Author(s):  
K.W. Lee ◽  
R.H. Meints ◽  
D. Kuczmarski ◽  
J.L. Van Etten
Keyword(s):  
Time Course ◽  
Reciprocal Cross ◽  
Ultrastructural Level ◽  
Ultrastructural Changes ◽  
Symbiotic Relationship ◽  
Cell Recognition ◽  
Green Hydra ◽  
Different Strains ◽  
Hydra Viridis

The physiological, biochemical, and ultrastructural aspects of the symbiotic relationship between the Chlorella-like algae and the hydra have been intensively investigated. Reciprocal cross-transfer of the Chlorellalike algae between different strains of green hydra provide a system for the study of cell recognition. However, our attempts to culture the algae free of the host hydra of the Florida strain, Hydra viridis, have been consistently unsuccessful. We were, therefore, prompted to examine the isolated algae at the ultrastructural level on a time course.

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