Study of porous cellulose beads as a dye-ligand matrix. Effect of protein admixtures and concentration of immobilized dye in the quantitative analysis of lactate dehydrogenase: Cibacron blue interaction

1990 ◽  
Vol 55 (2) ◽  
pp. 581-586 ◽  
Author(s):  
Danica Mislovičová ◽  
Peter Gemeiner ◽  
Viera Ďurišová
Keyword(s):  
Quantitative Analysis ◽  
Lactate Dehydrogenase ◽  
Matrix Effect ◽  
Dissociation Constants ◽  
Cibacron Blue ◽  
Blue Dextran ◽  
Nonspecific Interaction ◽  
The Matrix ◽  
Bead Cellulose ◽  
Total Interaction

The effect of protein admixtures and concentration of immobilized dye on the interaction of lactate dehydrogenase (LDH) with Cibacron Blue-bead cellulose has been studied by means of zonal chromatography with Cibacron Blue-dextran T 10 as the mobile ligand. The influence of both variables on the values of dissociation constants of complexes immobilized dye-LDH (KI-L) and mobile dye-LDH (KM-L) can be summarized as follows: The values of KI-L remained practically constant and were found to be lower than those of KM-L. The differences found in KI-L and KM-L values should help to estimate the contribution of nonspecific interaction of the matrix to the total interaction between dye-ligand and the enzyme.

Substitution–dilution method to correct the matrix effect in multi-element quantitative analysis by X-ray fluorescence

2001 ◽  
Vol 56 (2) ◽  
pp. 187-201 ◽  
Author(s):  
F Bosch-Reig ◽  
J.V Gimeno-Adelantado ◽  
S Sánchez-Ramos ◽  
D.J Yusá-Marco ◽  
F Bosch-Mossi
Keyword(s):  
Quantitative Analysis ◽  
Matrix Effect ◽  
Dilution Method ◽  
X Ray ◽  
The Matrix

Competitive elution of lactate dehydrogenase from Cibacron Blue—bead cellulose with Cibacron Blue—dextrans

1990 ◽  
Vol 510 ◽  
pp. 197-204 ◽  
Author(s):  
Danica Mislovičová ◽  
Peter Gemeiner ◽  
Eva Stratilová ◽  
Marta Horváthova
Keyword(s):  
Lactate Dehydrogenase ◽  
Cibacron Blue ◽  
Bead Cellulose

Quantitative Analysis of LIBS Reduces the Matrix Effect Methods

2013 ◽  
Vol 313-314 ◽  
pp. 579-582
Author(s):  
You Liang Yang ◽  
Jun Xiang Li ◽  
Fan Wei Meng ◽  
Cui Hong Ma
Keyword(s):  
Quantitative Analysis ◽  
Matrix Effect ◽  
Correction Method ◽  
Analysis Method ◽  
Network Reduction ◽  
Breakdown Spectroscopy ◽  
Laser Induced Breakdown ◽  
The Matrix ◽  
Quantitative Analysis Method ◽  
The Impact

This paper introduced the principle about the technology of Laser-induced Breakdown Spectroscopy (LIBS) of quantitative analysis. It was reviewed about the quantitative analysis of LIBS reduced method of matrix. The reason of cause matrix effect was not clear, but the matrix effect on the LIBS quantitative analysis of the impact can not be ignored. The LIBS quantitative analysis method was divided into two categories: one was based on the calibration curve with the mathematical matrix correction method; the other was combined with neural network reduction method of matrix. This paper was introduced for the two categories of methods, and gives an example to explain.

Investigation of the Matrix Effect on the Accuracy of Quantitative Analysis of Trace Metals in Liquids Using Laser-Induced Breakdown Spectroscopy with Solid Substrates

2016 ◽  
Vol 70 (12) ◽  
pp. 2016-2024 ◽  
Author(s):  
Junshan Xiu ◽  
Lili Dong ◽  
Hua Qin ◽  
Yunyan Liu ◽  
Jin Yu
Keyword(s):  
Quantitative Analysis ◽  
Trace Metals ◽  
Matrix Effect ◽  
Solid Substrate ◽  
Laser Induced Breakdown Spectroscopy ◽  
Paper Substrate ◽  
Viscous Liquids ◽  
Breakdown Spectroscopy ◽  
Laser Induced Breakdown ◽  
The Matrix

The detection limit of trace metals in liquids has been improved greatly by laser-induced breakdown spectroscopy (LIBS) using solid substrate. A paper substrate and a metallic substrate were used as a solid substrate for the detection of trace metals in aqueous solutions and viscous liquids (lubricating oils) respectively. The matrix effect on quantitative analysis of trace metals in two types of liquids was investigated. For trace metals in aqueous solutions using paper substrate, the calibration curves established for pure solutions and mixed solutions samples presented large variation on both the slope and the intercept for the Cu, Cd, and Cr. The matrix effects among the different elements in mixed solutions were observed. However, good agreement was obtained between the measured and known values in real wastewater. For trace metals in lubricating oils, the matrix effect between the different oils is relatively small and reasonably negligible under the conditions of our experiment. A universal calibration curve can be established for trace metals in different types of oils. The two approaches are verified that it is possible to develop a feasible and sensitive method with accuracy results for rapid detection of trace metals in industrial wastewater and viscous liquids by laser-induced breakdown spectroscopy.

Correction of Matrix Effect in Multielemental Quantitative Analysis by X-Ray Fluorescence Spectroscopy Using the Linear Behavior in the Analytical Range of the Substitution-Dilution Method

2002 ◽  
Vol 56 (1) ◽  
pp. 58-61
Author(s):  
F. Bosch-Reig ◽  
J. V. Gimeno-Adelantado ◽  
S. Sánchez-Ramos ◽  
D. J. Yusá-Marco ◽  
F. Bosch-Mossi ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Matrix Effect ◽  
Linear Range ◽  
Factor H ◽  
Dilution Method ◽  
X Ray ◽  
Linear Behavior ◽  
Relative Standard ◽  
The Matrix ◽  
Analytical Range

This paper is an analytical study of the possibility of applying the linear range of the substitution-dilution method to correct the matrix effect in quantitative analysis by X-ray fluorescence (XRF) spectroscopy. The analytical range is obtained from a series of samples prepared in the form of glass discs by substituting the unknown sample with a standard sample (substitution factor, h) including a diluent-melt. In general, the substitution-dilution method is hyperbolic in character and therefore the diluent is required to ensure linear behavior between If vs. h in the experimental range. The linear range is located between the concentrations of standard and unknown for each element analyzed. This linear model makes it possible to correct the matrix effect in quantitative analysis by XRF using a single multi-elemental standard for different types of samples with a complex matrix, such as geologicals and cements. The results found for Si, Ti, Al, Fe, Mn, Ca, K, and P in soil and sediment samples and Si, Fe, Al, Ca, and K in cements (white and gray) are statistically satisfactory. Thus, the mean relative standard deviation calculated for all analytes in each sample was: ±4.0% and ±5.0% in soils; ±5.0% in sediments; and ±6.0% or ±3.0% in cements, white and gray, respectively.

Interaction of lactate dehydrogenase isoenzymes with ligands

1988 ◽  
Vol 53 (8) ◽  
pp. 1857-1861 ◽  
Author(s):  
Jana Barthová ◽  
Jana Kučerová ◽  
Sylva Leblová
Keyword(s):  
Lactate Dehydrogenase ◽  
Enzyme Inhibitor ◽  
Dissociation Constants ◽  
Physiological Role ◽  
Competitive Inhibitor ◽  
Kinetic Measurements ◽  
Cibacron Blue ◽  
Michaelis Constants ◽  
Sepharose 4B ◽  
Lactate Dehydrogenase Isoenzymes

Isoenzymes of bovine lactate dehydrogenase (H4, H3M, and H2M2) were prepared by affinity chromatography on a 5'-AMP-Sepharose 4B column. The interaction of isoenzymes with two ligands, coenzyme NADH and the competitive inhibitor Cibacron Blue F3GA was followed by means of kinetic measurements and by affinity electrophoresis. The Michaelis constants of NADH were compared with the inhibition constants of Cibacron Blue and dissociation constants of enzyme-inhibitor complexes. It was found that the M subunit of lactate dehydrogenase exhibits always higher affinity both to NADH and Cibacron Blue in comparison to the H subunit. This finding corresponds to the physiological role of lactate dehydrogenase isoenzymes.

Lateral resolution of the electron microbeam analysis

1978 ◽  
Vol 36 (1) ◽  
pp. 542-543
Author(s):  
H.J. Dudek
Keyword(s):  
Quantitative Analysis ◽  
Depth Resolution ◽  
Lateral Resolution ◽  
Cylindrical Particle ◽  
Correction Procedure ◽  
X Ray ◽  
Element Concentrations ◽  
Microbeam Analysis ◽  
The Matrix

The chemical inhomogenities in modern materials such as fibers, phases and inclusions, often have diameters in the region of one micrometer. Using electron microbeam analysis for the determination of the element concentrations one has to know the smallest possible diameter of such regions for a given accuracy of the quantitative analysis.In th is paper the correction procedure for the quantitative electron microbeam analysis is extended to a spacial problem to determine the smallest possible measurements of a cylindrical particle P of high D (depth resolution) and diameter L (lateral resolution) embeded in a matrix M and which has to be analysed quantitative with the accuracy q. The mathematical accounts lead to the following form of the characteristic x-ray intens ity of the element i of a particle P embeded in the matrix M in relation to the intensity of a standard S

A Modified QuEChERS Extraction and LC-MS/MS Method for the Determination of Pesticide Residues in Curry Leaves (Murraya koenigii)

2019 ◽  
Vol 6 (1) ◽  
pp. 30-41
Author(s):  
Ranjith Arimboor ◽  
Karunkara Ramakrishna Menon ◽  
Natarajan Ramesh Babu ◽  
Haneesh Chandran
Keyword(s):  
Sample Preparation ◽  
Matrix Effect ◽  
Retention Time ◽  
Pesticide Residues ◽  
Mobile Phase ◽  
Preparation Technique ◽  
Modified Quechers ◽  
Curry Leaves ◽  
Ms Analysis ◽  
The Matrix

Background:Increased consumer demand for curry leaves free from pesticides demands fast and reliable analytical methods for the analysis of pesticide residues.Objective:The optimization of a QuEChERS based sample preparation technique with improved analytical accuracy by removing interfering matrix components for LC-MS/MS analysis of pesticide residues from curry leaves.Methods:A modified QuEChERS solid phase extraction method was developed and validated for the analysis of 26 pesticides in fresh and dried curry leaves. The effects of the sample preparation steps and column retention time on the matrix suppression of analyte ions were also evaluated.Results:Validation parameters were found within an acceptable range. The matrix effect evaluation studies showed that the QuEChERS sample preparation was able to minimize the ion suppression of analytes due to co-eluting matrix of components and that a d-SPE clean up step had major role in reducing matrix effect. The gradient mobile phase with longer retention time for analytes resulted in comparatively lesser matrix effects than the isocratic mobile phase of non-polar nature. Even after the clean up, a considerable number of compounds had more than 20% reduction in their MS response in the gradient mobile phase.Conclusion:This study emphasized the need of proper sample clean up before a LC-MS/MS analysis and the usage of matrix matched standards and mobile phase that ultimately results in an appropriate analyte separation in reasonable retention times.

On the matrix-effect in the excitation of the atomic emission spectra of magnesites

1965 ◽  
Vol 30 (4) ◽  
pp. 1303-1310 ◽  
Author(s):  
M. Matherny ◽  
N. Pliešovská ◽  
Ž. Rybárová
Keyword(s):  
Matrix Effect ◽  
Emission Spectra ◽  
Atomic Emission ◽  
The Matrix
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