OP0078 Presence of an Interferon Signature in Anti-Nuclear Antibody Positive Individuals Prior to the Onset of Systemic Autoimmune Rheumatic Disease

Objective. To analyze the clinical value of anti-DFS70 antibodies in a cohort of patients undergoing routine antinuclear antibodies (ANAs) testing.Methods. Sera with a dense fine speckled (DFS) indirect immunofluorescence (IIF) pattern from 100 consecutive patients and 100 patients with other IIF patterns were tested for anti-DFS70 antibodies by a novel chemiluminescence immunoassay (CIA) and for ANA by ANA Screen ELISA (both INOVA).Results. Among the 100 patients with a DFS IIF pattern, 91% were anti-DFS70 positive by CIA compared to 3% in the comparator group(P<0.0001). The CIA and IIF titers of anti-DFS antibodies were highly correlated (rho = 0.89). ANA by ELISA was positive in 35% of patients with the DFS IIF pattern as compared to 67% of patients with other patterns(P<0.0001). Only 12.0% of patients with DFS pattern and 13.4% with DFS pattern and anti-DFS70 antibodies detected by CIA had systemic autoimmune rheumatic disease (SARD). Only 5/91 (5.5%) patients with anti-DFS70 antibodies had SARD and their sera were negative on the ANA Screen ELISA.Conclusion. Although anti-DFS70 antibodies cannot exclude the presence of SARD, the likelihood is significantly lower than in patients with other IIF patterns and should be included in test algorithms for ANA testing.

Download Full-text

Undifferentiated vasculitis or an evolving systemic autoimmune rheumatic disease?

Oxford Medical Case Reports ◽

10.1093/omcr/omw066 ◽

2016 ◽

Vol 2016 (8) ◽

pp. omw066

Author(s):

Nafeesah Fatimah ◽

Ahmad Ussaid ◽

Aflak Rasheed

Keyword(s):

Rheumatic Disease ◽

Autoimmune Rheumatic Disease ◽

Systemic Autoimmune Rheumatic Disease

Download Full-text

Modeling systemic autoimmune rheumatic disease in rats under the adverse weather conditions.

Medicni perspektivi (Medical perspectives) ◽

10.26641/2307-0404.2017.1.100896 ◽

2017 ◽

Vol 22 (1) ◽

pp. 38-43

Author(s):

Ye. D. Yegudina ◽

О. V. Synyachenko ◽

О. О. Khanyukov ◽

V. І. Suiarko ◽

G. S. Тaktashov

Keyword(s):

Rheumatic Disease ◽

Weather Conditions ◽

Autoimmune Rheumatic Disease ◽

Adverse Weather Conditions ◽

Systemic Autoimmune Rheumatic Disease ◽

Adverse Weather

Download Full-text

Anti-DFS70 Antibodies for Differentiating Systemic Autoimmune Rheumatic Disease in Patients with Positive ANA Tests: A Systematic Review and Meta-Analysis

Diagnostics ◽

10.3390/diagnostics11091592 ◽

2021 ◽

Vol 11 (9) ◽

pp. 1592

Author(s):

Chiao-Feng Cheng ◽

Ming-Chieh Shih ◽

Ting-Yuan Lan ◽

Ko-Jen Li

Keyword(s):

Confidence Interval ◽

Rheumatic Disease ◽

Meta Analysis ◽

Cochrane Library ◽

Test Accuracy ◽

Summary Receiver Operating Characteristic ◽

Bivariate Model ◽

Autoimmune Rheumatic Disease ◽

Systemic Autoimmune Rheumatic Disease ◽

Test Result

Anti-DFS70 antibodies have been proposed as a marker to exclude systemic autoimmune rheumatic disease (SARD). We conducted this systematic diagnostic test accuracy review and meta-analysis to determine the performance of anti-DFS70 antibodies in patients with a positive anti-nuclear antibody (ANA) test result to exclude SARD. We searched PubMed, Embase, Web of Science, Scopus and the Cochrane Library up to 22 February 2021, and included studies examining the diagnostic accuracy of anti-DFS70 antibodies in patients with a positive ANA test result. The results were pooled using a hierarchical bivariate model and plotted in summary receiver operating characteristic curves. R software and Stata Statistical Software were used for the statistical analysis. Eight studies with 4168 patients were included. The summary sensitivity was 0.19 (95% confidence interval: 0.12–0.28) and the specificity was 0.93 (95% confidence interval: 0.88–0.96). The area under the curve was 0.69 (95% confidence interval: 0.64–0.72). The meta-regression analysis showed that targeting only ANA-associated rheumatic disease was associated with higher specificity. In addition, the studies with a non-SARD prevalence of <80% and using a chemiluminescence assay were associated with higher specificity. Anti-DFS70 antibodies have high specificity for the exclusion of SARD among patients presenting with a positive ANA test, but the sensitivity is low.

Download Full-text