G203(P) Transient Temperature Gel Electrophoresis of Stool Samples of Preterm Infants in a Multicentre Observational Study

Approximately over 500,000 children die annually due to severe dehydrating diarrhea caused by rotaviruses. This work investigated rotavirus infection among children less than 5 years with diarrhea in Lagos and determined the circulating electropherotypes and genotypes of the virus isolates. Three hundred and two (n=302) stool samples from children below 60 months were collected from different hospitals and health care centers in Lagos and subjected to enzyme immunoassay (EIA) to determine the presence of Group A rotavirus, RT-PCR to determine the G-types, and polyacrylamide gel electrophoresis (PAGE) to determine the electropherotypes. The results show that 60.3% of the samples showed distinct rotavirus RNA migration pattern, having long electropherotypes (55.3%) of seven variations dominating over the short electropherotypes (44.5%). Six different G-types were detected (G1, G2, G3, G4, G9, and G12). Serotypes G1 and G12 showed long electropherotypic pattern while G2, G3, and G9 exhibited either short or long electropherotype. All G4 detected show short electropherotypic pattern. In conclusion, information on the genomic diversity and RNA electropherotypes of rotaviruses detected in children with diarrhea in Lagos is reported in this study.

Download Full-text

N-terminal pro-B-type natriuretic peptide as a marker of ductal haemodynamic significance in preterm infants: a prospective observational study

Archives of Disease in Childhood - Fetal and Neonatal Edition ◽

10.1136/adc.2007.119701 ◽

2007 ◽

Vol 92 (5) ◽

pp. F421-F422 ◽

Cited By ~ 30

Author(s):

A. F El-Khuffash ◽

M. Amoruso ◽

M. Culliton ◽

E. J Molloy

Keyword(s):

Observational Study ◽

Natriuretic Peptide ◽

Preterm Infants ◽

Prospective Observational Study

Download Full-text

Characterization of rotavirus electropherotypes excreted by symptomatic and asymptomatic infants

Epidemiology and Infection ◽

10.1017/s0950268800056557 ◽

1991 ◽

Vol 106 (1) ◽

pp. 189-198 ◽

Cited By ~ 4

Author(s):

J. Fernández ◽

A. M. Sandino ◽

J. Pizarro ◽

L. F. Avendaño ◽

J. M. Pizarro ◽

...

Keyword(s):

Enzyme Immunoassay ◽

Gel Electrophoresis ◽

Polyacrylamide Gel Electrophoresis ◽

Polyacrylamide Gel ◽

Mixed Infections ◽

Migration Patterns ◽

Virulent Strains ◽

Human Rotavirus ◽

Stool Samples

SUMMARYHuman rotavirus isolates from 1100 stool samples were analyzed by polyacrylamide gel electrophoresis, and 48 different migration patterns were detected. Heterogeneity in the migration of segment 10 was observed in both long and short electropherotypes in which three long and two short patterns were identified. In spite of these variations all short and long electropherotypes were subgrouped by enzyme immunoassay as subgroups I and II respectively. Mixed infections were detected in 17% of cases and the subgrouping correlated with the corresponding electropherotypes. The same electropherotypes were present in severe, mild and asymptomatic cases and no electropherotype was particularly associated with greater virulence. Furthermore, the electropherotypes isolated from nosocomial asymptomatic cases were the same as those detected from those admitted with severe diarrheoa. It seems unlikely that electropherotyping can be used to identify more virulent strains of rotavirus.

Download Full-text