Multi-valvular infective endocarditis from Gemella morbillorum

2021 ◽  
Vol 14 (7) ◽  
pp. e242093
Author(s):  
Anish Kumar Desai ◽  
Erin Murchan Bonura
Keyword(s):  
Infectious Endocarditis ◽  
Culture Methods ◽  
Aortic Valves ◽  
Gastrointestinal Malignancy ◽  
Pulmonic Valve ◽  
Ionization Time ◽  
Flight Mass Spectrometry ◽  
Occult Bleeding ◽  
Standard Culture ◽  
Ongoing Evaluation

Gemella morbillorum is increasingly implicated in infectious endocarditis. Our patient presented with anaemia and renal failure with evidence of infarcts and embolic disease. He was found to have endocarditis with an organism that could not speciate with standard culture methods requiring matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF) for identification and susceptibilities. While involvement of mitral and aortic valves can be expected with Gemella, he had rare involvement of the pulmonic valve in a structurally normal heart. Although bacteriological cure was achieved, due to the locally destructive nature of Gemella, he ultimately required valve replacements for heart failure resolution. Workup for commonly implicated pathologies associated with G. morbillorum led to suspicion of gastrointestinal malignancy with findings of occult bleeding prompting an ongoing evaluation. With improved access to advanced diagnostics, G. morbillorum has been increasingly identified in infectious endocarditis. Given its destructive nature, it is important for clinicians to consider this organism is difficult to identify isolates.

Printculture of Surgical Pathology and Autopsy Specimens

2019 ◽  
Vol 152 (6) ◽  
pp. 747-756
Author(s):  
Phillip D McMullen ◽  
Vera Tesic ◽  
Peter Pytel
Keyword(s):  
Laser Desorption ◽  
Surgical Pathology ◽  
Laser Desorption Ionization ◽  
Culture Methods ◽  
Ionization Time ◽  
Maldi Tof ◽  
Standard Culture ◽  
Autopsy Specimens ◽  
True Infection ◽  
Matrix Assisted Laser

Abstract Objectives Printculture is a method of microbiologic assessment previously described for use in the autopsy setting. We sought to compare printculture of surgical and autopsy pathology specimens to standard microbiology culture using matrix-assisted laser desorption/ionization–time of flight (MALDI-TOF)–based colony identification. Methods Printculture was performed on 18 frozen samples with corresponding standard culture results. The results of MALDI-TOF identification of colonies recovered by printculture were compared with standard cultures, and percent concordance was calculated. Results There was 95.8% concordance to standard culture methods for cases with infections and 100% concordance for cases without infection. The pattern of growth was found to aid in the distinction between contamination and true infection. Conclusions Printculture allows the identification of microorganisms from routinely frozen tissues and provides a bridge between microbiology and histomorphology through the identification of associated histologic features of infection. This technique can be successfully integrated into autopsy and surgical pathology workup of potentially infected tissues.

Upper Respiratory Microbiota in Relation to Ear and Nose Health Among Australian Aboriginal and Torres Strait Islander Children

2021 ◽  
Author(s):  
Andrea Coleman ◽  
Seweryn Bialasiewicz ◽  
Robyn L Marsh ◽  
Eva Grahn Håkansson ◽  
Kyra Cottrell ◽  
...  
Keyword(s):  
Healthy Children ◽  
Upper Respiratory Tract ◽  
Indigenous Australian ◽  
Ionization Time ◽  
Australian Aboriginal ◽  
Flight Mass Spectrometry ◽  
Upper Respiratory ◽  
Corynebacterium Pseudodiphtheriticum ◽  
Nasal Microbiota ◽  
Respiratory Microbiota

Abstract Background We explored the nasal microbiota in Indigenous Australian children in relation to ear and nasal health. Methods In total, 103 Indigenous Australian children aged 2–7 years (mean 4.7 years) were recruited from 2 Queensland communities. Children’s ears, nose, and throats were examined and upper respiratory tract (URT) swabs collected. Clinical histories were obtained from parents/medical records. URT microbiota were characterized using culturomics with Matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) identification. Real-time PCR was used to quantify otopathogen (Haemophilus influenzae, Streptococcus pneumoniae, and Moraxella catarrhalis) loads and detect respiratory viruses. Data were analyzed using beta diversity measures, regression modeling, and a correlation network analysis. Results Children with historical/current otitis media (OM) or URT infection (URTI) had higher nasal otopathogen detection and loads and rhinovirus detection compared with healthy children (all P < .04). Children with purulent rhinorrhea had higher nasal otopathogen detection and loads and rhinovirus detection (P < .04) compared with healthy children. High otopathogen loads were correlated in children with historical/current OM or URTI, whereas Corynebacterium pseudodiphtheriticum and Dolosigranulum pigrum were correlated in healthy children. Conclusions Corynebacterium pseudodiphtheriticum and D. pigrum are associated with URT and ear health. The importance of the main otopathogens in URT disease/OM was confirmed, and their role relates to co-colonization and high otopathogens loads.

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