Effect of dietary cholesterol on plasma cholesterol concentration.

Background Hypercholesterolemia (plasma cholesterol concentration ≥5.2 mmol/L) is a risk factor for cardiovascular disease and stroke. Many different cholesterol self-tests are readily available at general stores, pharmacies and web shops. However, there is limited information on their analytical and diagnostic performance. Methods We included 62 adult patients who required a lipid panel measurement (cholesterol, high-density lipoprotein (HDL), triglycerides and LDLcalc) for routine care. The performance of five different cholesterol self-tests, three quantitative meters ( Roche Accutrend Plus, Mission 3-in-1 and Qucare) and two semi-quantitative strip tests ( Veroval and Mylan MyTest), was assessed according to the manufacturers’ protocol. Results The average plasma cholesterol concentration was 5.2 ± 1.2 mmol/L. The mean absolute relative difference (MARD) of the five cholesterol self-tests ranged from 6 ± 5% ( Accutrend Plus) to 20 ± 12% ( Mylan Mytest). The Accutrend Plus cholesterol meter showed the best diagnostic performance with a 92% sensitivity and 89% specificity. The Qucare and Mission 3-in-1 are able to measure HDL concentrations and can thus provide a cholesterol:HDL ratio. The Passing-Bablok regression analyses for the ratio showed poor performance in both self-tests ( Mission 3-in-1: y = 1.62x–1.20; Qucare: y = 0.61x + 1.75). The Accutrend Plus is unable to measure the plasma high-density lipoprotein concentration. Conclusions/interpretation: The Accutrend Plus cholesterol meter (Roche) had excellent diagnostic and analytic performance. However, several of the commercially-available self-tests had considerably poor accuracy and diagnostic performance and therefore do not meet the required qualifications, potentially leading to erroneous results. Better regulation, standardization and harmonization of cholesterol self-tests is warranted.

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Fat Containing Stearic Acid Increases Fecal Neutral Steroid Excretion and Catabolism of Low Density Lipoproteins Without Affecting Plasma Cholesterol Concentration in Hamsters Fed a Cholesterol-Containing Diet

Journal of Nutrition ◽

10.1093/jn/123.10.1693 ◽

1993 ◽

Vol 123 (10) ◽

pp. 1693-1702 ◽

Cited By ~ 41

Author(s):

Katsumi Imaizumi ◽

Koji Abe ◽

Chiho Kuroiwa ◽

Michihiro Sugano

Keyword(s):

Stearic Acid ◽

Plasma Cholesterol ◽

Low Density Lipoproteins ◽

Cholesterol Concentration ◽

Low Density ◽

Plasma Cholesterol Concentration ◽

Neutral Steroid ◽

Steroid Excretion

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Cholesterol Measurement in Serum and in Plasma

Clinical Chemistry ◽

10.1093/clinchem/10.7.619 ◽

1964 ◽

Vol 10 (7) ◽

pp. 619-626 ◽

Cited By ~ 11

Author(s):

Francisco Grande ◽

Donald S Amatuzio ◽

Shohachi Wada

Keyword(s):

Citric Acid ◽

Water Distribution ◽

Plasma Cholesterol ◽

Cholesterol Concentration ◽

Sodium Oxalate ◽

Plasma Cholesterol Concentration ◽

The Mean ◽

Cholesterol Measurement

Abstract The effect on the plasma cholesterol concentration of various anticoagulants added to the blood in dry form, as compared with that of serum, has been studied. The amounts of anticoagulants used, per 5 ml. of blood, were: heparin, 2.5 mg.; oxalate mixture, 12 mg.; tetrasodium EDTA, 5 mg.; sodium oxalate, 12mg.; and ACD mixture, 5.3 mg. citric acid, 15.8 mg. Na citrate, and 17.6 mg. dextrose. All these anticoagulants caused a decrease in the plasma cholesterol concentration. The mean cholesterol decrease (serum minus plasma) produced by heparin was only 4 mg./100 ml. (S.E., ± 1.24), but this difference was statistically significant. When the heparin was prepared by drying a solution of heparin in 0.9% NaCl a greater difference between serum and plasma was observed. The cholesterol difference (serum minus plasma) producd by Na oxalate increases with increasing amounts of anticoagulant. The decrease in plasma cholesterol concentration produced by the anticoagulants can be explained by changes of water distribution between cells and plasma, reflected by changes of hematocrit produced by the anticoagulant.

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Neutrophil enhancement of reperfusion-induced capillary fluid filtration associated with hypercholesterolemia

AJP Heart and Circulatory Physiology ◽

10.1152/ajpheart.1996.271.5.h1755 ◽

1996 ◽

Vol 271 (5) ◽

pp. H1755-H1761 ◽

Cited By ~ 2

Author(s):

N. R. Harris ◽

D. N. Granger

Keyword(s):

Capillary Permeability ◽

Plasma Cholesterol ◽

Cholesterol Concentration ◽

Barrier Dysfunction ◽

Plasma Cholesterol Concentration ◽

Fluid Filtration ◽

Before And After ◽

Activated Neutrophils ◽

Capillary Fluid ◽

Capillary Response

Fluid filtration rate (Jv/S) from individual mesenteric capillaries in normocholesterolemic and hypercholesterolemic rats was measured before and after 30 min each of ischemia and reperfusion (I/R). The median I/R-induced increase in Jv/S (I/R vs. baseline) was 44% in normocholesterolemic rats (n = 11) and 97% in hypercholesterolemic rats (n = 11). A positive correlation slope of 0.20% per mg/dl resulted when the percent Jv/S increase vs. plasma cholesterol concentration (P = 0.02) was plotted, demonstrating that hypercholesterolemia enhances the capillary response to I/R. Because microvascular pressure did not change significantly after I/R in either group of rats, the increments in Jv/S likely reflect increased capillary permeability. In hypercholesterolemic rats rendered neutropenic with antineutrophil serum, I/R did not elicit a significant increase in Jv/S, suggesting that activated neutrophils mediate the exaggerated endothelial barrier dysfunction associated with hypercholesterolemia.

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