scholarly journals Normal platelet function in platelet concentrates requires non-platelet cells: a comparative in vitro evaluation of leucocyte-rich (type 1a) and leucocyte-poor (type 3b) platelet concentrates

2016 ◽  
Vol 2 (1) ◽  
pp. e000071 ◽  
Author(s):  
William R Parrish ◽  
Breana Roides ◽  
Julia Hwang ◽  
Michael Mafilios ◽  
Brooks Story ◽  
...  
Keyword(s):  
Platelet Function ◽  
Platelet Concentrates ◽  
In Vitro Evaluation ◽  
Normal Platelet

Human Platelet Acetylcholinesterase: The Effects of Anticholinesterases on Platelet Function

1979 ◽  
Vol 42 (05) ◽  
pp. 1615-1619 ◽  
Author(s):  
Martin J Smith ◽  
Boyd Braem ◽  
Kent D Davis
Keyword(s):  
Platelet Function ◽  
Ache Activity ◽  
Room Temperature ◽  
Platelet Rich Plasma ◽  
Complete Inhibition ◽  
Clot Retraction ◽  
Plasma Clot ◽  
Normal Platelet ◽  
Aggregation Factor

SummaryPlatelet acetylcholinesterase (AChE) activity was measured in gel-filtered platelet preparations. Three different anticholinesteratic agents (eserine, neostigmine, and diiso- propylphosphorofluoridate) at final concentrations of 10 μM caused complete inhibition of AChE activity after 30 min incubation at room temperature with either platelet-rich plasma or gel-filtered platelets. Complete inhibition of platelet AChE had no effect on platelet aggregation, factor-3 availability, and plasma clot retraction. We conclude that platelet membrane AChE activity is not required for normal platelet function as measured by these in vitro parameters.

In vitro Evaluation of Buffy-Coat-Derived Platelet Concentrates Stored in a Synthetic Medium

Vox Sanguinis ◽  
1991 ◽  
Vol 60 (1) ◽  
pp. 16-22 ◽  
Author(s):  
R. Fijnheer ◽  
H.A. Veldman ◽  
A.J.M. Eertwegh ◽  
C.W.N. Gouwerok ◽  
C.H.E. Homburg ◽  
...  
Keyword(s):  
Synthetic Medium ◽  
Buffy Coat ◽  
Platelet Concentrates ◽  
In Vitro Evaluation

Quality Control of Platelet Concentrates by the Thrombostat 4000

1995 ◽  
Vol 21 (S 02) ◽  
pp. 91-95 ◽  
Author(s):  
Markus Böck ◽  
Joachim Groh ◽  
Anle Glaser ◽  
Klaus Storck ◽  
Michael Kratzer ◽  
...  
Keyword(s):  
Quality Control ◽  
Platelet Function ◽  
Test System ◽  
Serotonin Release ◽  
Small Sample ◽  
Platelet Concentrates ◽  
Platelet Function Tests ◽  
Special Equipment ◽  
Function Tests

Quality control of platelet concentrates (PC) is an important prerequisite for good transfusion praxis. However, direct measurement of platelet function is complex, since available methods (e.g. aggregometry, serotonin release) are time consuming and require special equipment. Therefore a test system is needed, which is easy to handle, fast, and achieves reliable results. The present paper compares the results of conventional platelet function tests with those of a modified in-vitro bleeding test (IVBT) (Thrombostat 4000) in liquid-stored and cryopreserved PCs. A high correlation between aggregometry, serotonin release, GMP 140 expression upon stimulation, and IVBT was demonstrated. Therefore IVBT seems to be a good alternative to the conventional platelet function tests for quality control of PCs. In addition, a good correlation between the results of IVBT of patients’ blood after PC transfusion and IVBT of patients blood before transfusion supplemented with platelets of the respective PC could be found. Therefore IVBT seems to be able to predict PC transfusion success. However, since these data were obtained in a small sample undergoing bone marrow transplantation, further studies are needed to verify this hypothesis.

In vitro evaluation of platelet concentrates, prepared from pooled buffy coats, stored for 8 days after filtration

Transfusion ◽  
1994 ◽  
Vol 34 (4) ◽  
pp. 311-316 ◽  
Author(s):  
MN Boomgaard ◽  
AM Joustra-Dijkhuis ◽  
CW Gouwerok ◽  
I Steneker ◽  
HW Reesink ◽  
...  
Keyword(s):  
Platelet Concentrates ◽  
In Vitro Evaluation

In vitro study of platelet function confirms the contribution of the ultraviolet B (UVB) radiation in the lesions observed in riboflavin/UVB-treated platelet concentrates

Transfusion ◽  
2015 ◽  
Vol 55 (9) ◽  
pp. 2219-2230 ◽  
Author(s):  
Mélanie Abonnenc ◽  
Giona Sonego ◽  
David Crettaz ◽  
Alessandro Aliotta ◽  
Michel Prudent ◽  
...  
Keyword(s):  
Platelet Function ◽  
In Vitro Study ◽  
Ultraviolet B ◽  
Vitro Study ◽  
Uvb Radiation ◽  
Platelet Concentrates

scholarly journals The secreted tyrosine kinase VLK is essential for normal platelet activation and thrombus formation

2021 ◽  
Author(s):  
Leila Revollo ◽  
Glenn Merrill-Skoloff ◽  
Karen De Ceunynck ◽  
James R. Dilks ◽  
Mattia Bordoli ◽  
...  
Keyword(s):  
Tyrosine Kinase ◽  
Tyrosine Phosphorylation ◽  
Platelet Activation ◽  
Platelet Function ◽  
Secretory Pathway ◽  
Thrombus Formation ◽  
Extracellular Proteins ◽  
Normal Platelet

AbstractTyrosine phosphorylation of extracellular proteins is observed in cell cultures and in vivo, but little is known about the functional roles of tyrosine phosphorylation of extracellular proteins. Vertebrate Lonesome Kinase (VLK) is a broadly expressed secretory pathway tyrosine kinase present in platelet ɑ-granules. It is released from platelets upon activation and phosphorylates substrates extracellularly. Its role in platelet function, however, has not been previously studied. In human platelets, we identified phosphorylated tyrosines mapped to luminal or extracellular domains of transmembrane and secreted proteins implicated in the regulation of platelet activation. To determine the role of VLK in extracellular tyrosine phosphorylation and platelet function, we generated mice with a megakaryocyte/platelet-specific deficiency of VLK. Platelets from these mice are normal in abundance and morphology, but have dramatic changes in function both in vitro and in vivo. Resting and thrombin-stimulated VLK-deficient platelets demonstrate a significant decrease of several tyrosine phosphobands. Functional testing of VLK-deficient platelets shows decreased PAR4- and collagen-mediated platelet aggregation, but normal responses to ADP. Dense granule and α-granule release are reduced in these platelets. Furthermore, VLK-deficient platelets exhibit decreased PAR4-mediated Akt (S473) and Erk1/2(T202/Y204) phosphorylation, indicating altered proximal signaling. In vivo, mice lacking VLK in megakaryocytes/platelets demonstrate strongly reduced platelet accumulation and fibrin formation following laser-injury of cremaster arterioles compared to controls. These studies demonstrate that the secretory pathway tyrosine kinase VLK is critical for stimulus-dependent platelet activation and thrombus formation, providing the first evidence that a secreted protein kinase is required for normal platelet function.

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