Venipuncture does not affect adrenocorticotropic hormone concentration in horses: TABLE 1:

2015 ◽  
Vol 177 (9) ◽  
pp. 223.2-223 ◽  
Author(s):  
N. Fouché ◽  
J. H. van der Kolk ◽  
R. M. Bruckmaier ◽  
I. Luz ◽  
G. Foerster ◽  
...  
Keyword(s):  
Adrenocorticotropic Hormone ◽  
Hormone Concentration

scholarly journals Feline plasma adrenocorticotropic hormone: validation of a chemiluminescent assay and concentrations in cats with hypercortisolism, primary hypoadrenocorticism and other diseases

2020 ◽  
pp. 1098612X2092568
Author(s):  
Antonio M Tardo ◽  
Claudia E Reusch ◽  
Sara Galac ◽  
Sofia Fornetti ◽  
Alessandro Tirolo ◽  
...  
Keyword(s):  
Adrenocorticotropic Hormone ◽  
Reference Interval ◽  
Robust Methods ◽  
Hormone Concentration ◽  
Plasma Acth ◽  
Naturally Occurring ◽  
Assay Precision ◽  
Diagnostic Work ◽  
Work Up ◽  
Interassay Precision

Objectives The aims of this study were to validate a commercially available chemiluminescent assay for measurement of feline plasma adrenocorticotropic hormone concentration (ACTH), to determine the normal reference interval (RI) of plasma ACTH in healthy cats, to assess plasma ACTH in cats with naturally occurring hypercortisolism (HC), primary hypoadrenocorticism (PH) and other diseases (OD), and to evaluate the effect of aprotinin on plasma ACTH degradation. Methods Forty healthy cats, 10 with HC, 11 with PH and 30 with OD, were included. The chemiluminescent enzyme immunometric assay was evaluated by measurement of intra-assay precision, interassay precision and linearity. The RI for plasma ACTH in healthy cats was established using robust methods. Plasma ACTH of samples collected with and without aprotinin, stored at 4°C and assayed over a 6-day period, was measured. Results The intra-assay coefficients of variance (CVs) ranged from 2.7% to 4.3% and interassay CVs from 3.3% to 10.7%. Dilution studies showed excellent accuracy (R2 >0.99). The RI for plasma ACTH in healthy cats was 32–370 pg/ml. Plasma ACTH was not significantly different between healthy cats and the OD group. Cats with pituitary-dependent hypercortisolism (PDH) and PH had significantly higher plasma ACTH than the other groups. Plasma ACTH did not show significant differences when samples collected with and without aprotinin were compared. Conclusions and relevance The Immulite chemiluminescent assay is a valid technique for measuring plasma ACTH in cats and the RI of plasma ACTH is quite wide. Owing to the low overlap between healthy or OD cats and cats with HC or PH, the measurement of plasma ACTH appears to be useful and should be included in the diagnostic work-up when HC or PH are suspected. Furthermore, the measurement of plasma ACTH may be an accurate test for differentiating PDH from adrenal-dependent hypercortisolism.

scholarly journals Plasma adrenocorticotropic hormone concentration in horses decreases after freezing for 60 days

2019 ◽  
Vol 31 (6) ◽  
pp. 856-858
Author(s):  
John C. Haffner ◽  
Dwana L. Neal ◽  
Rhonda M. Hoffman ◽  
Steven T. Grubbs
Keyword(s):  
Repeated Measures ◽  
Adrenocorticotropic Hormone ◽  
Mixed Model ◽  
Statistical Significance ◽  
Hormone Concentration ◽  
Plasma Acth ◽  
Acth Concentration ◽  
The Stability ◽  
Over Time

We investigated the stability of adrenocorticotropic hormone (ACTH) in plasma after freezing for different lengths of time. The plasma ACTH concentrations of 12 horses were measured on day 0 (baseline) and over time, after stimulation with thyrotropin-releasing hormone. Samples were stored at −80°C for 3, 7, 30, 60, and 90 d, or at −20°C for 3, 7, 30, and 60 d, or between ice packs at −20°C for 3 and 7 d prior to determination of ACTH concentration. ACTH concentrations were compared to baseline (non-frozen day 0 plasma) for each storage method using a mixed model with repeated measures in which each horse served as its own control and day was the repeated effect. Statistical significance was set at p ≤ 0.05, and 0.05 < p < 0.10 was considered a trend. Plasma ACTH frozen at −20°C or at −80°C resulted in degradation of ACTH compared to baseline samples at 60 and 90 d respectively. There was no degradation of ACTH after 7 d when stored between ice packs, or before 30 d at −20°C, or before 60 d at −80°C.

scholarly journals Relationship between endogenous plasma adrenocorticotropic hormone concentration and reproductive performance in Thoroughbred broodmares

2021 ◽  
Author(s):  
Takeru Tsuchiya ◽  
Ryusuke Noda ◽  
Hiroki Ikeda ◽  
Masaya Maeda ◽  
Fumio Sato
Keyword(s):  
Reproductive Performance ◽  
Adrenocorticotropic Hormone ◽  
Hormone Concentration

Effects of Pergolide Mesylate on Plasma Adrenocorticotropic Hormone Concentration in Horses with Pituitary Pars Intermedia Dysfunction

2013 ◽  
Vol 45 ◽  
pp. 19-19
Author(s):  
D.I. Rendle ◽  
E. Taylor ◽  
M. Duz ◽  
T.D. Parkin ◽  
V.E.N. Copas ◽  
...  
Keyword(s):  
Adrenocorticotropic Hormone ◽  
Pars Intermedia ◽  
Hormone Concentration

Variation in Plasma Adrenocorticotropic Hormone Concentration and Dexamethasone Suppression Test Results with Season, Age, and Sex in Healthy Ponies and Horses

2005 ◽  
Vol 19 (2) ◽  
pp. 217-222 ◽  
Author(s):  
Mark T. Donaldson ◽  
Sue M. McDonnell ◽  
Barbara J. Schanbacher ◽  
Stephen V. Lamb ◽  
Dianne McFarlane ◽  
...  
Keyword(s):  
Adrenocorticotropic Hormone ◽  
Dexamethasone Suppression Test ◽  
Test Results ◽  
Hormone Concentration ◽  
Suppression Test ◽  
Dexamethasone Suppression ◽  
Age And Sex

Adrenocorticotropin Production by a Mammary Carcinoma

1977 ◽  
Vol 35 ◽  
pp. 462-463
Author(s):  
K.S. McCarty ◽  
N.R. Wallace ◽  
W. Litaker ◽  
S. Wells ◽  
G. Eisenbarth
Keyword(s):  
Adrenocorticotropic Hormone ◽  
Small Cell ◽  
Hormone Production ◽  
Carcinoma Of The Breast ◽  
Ectopic Acth ◽  
Ultrastructural Studies ◽  
Subcutaneous Metastases ◽  
Ectopic Acth Production ◽  
The Right ◽  
Pituitary Carcinomas

The production of adrenocorticotropic hormone by non-pituitary carcinomas has been documented in several tumors, most frequently small cell carcinoma of the lung, islet cell carcinomas of the pancreas, thymomas and carcinoids. Electron microscopy of these tumors reveals typical membrane-limited "neurosecretory" granules. Confirmation of the granules as adrenocorticotropin (ACTH) requires the use of OsO4 as a primary fixative to give the characteristic cored granule appearance in conjunction with immunohistochemical demonstration of the hormone peptide. Because of the rarity of ectopic ACTH production by mammary carcinomas and the absence of appropriate ultrastructural studies in the two examples of such ectopic hormone production in the literature of which we are aware (1,2), we present biochemical and ultrastructural data from a carcinoma of the breast with apparent ACTH production.The patient had her primary tumor in the right breast in 1969. The tumor recurred as visceral and subcutaneous metastases in 1976 and again in 1977.

Morphometric SEM study of the effects of ACTH on endothelial fenestrae in the rat adrenal cortex

1984 ◽  
Vol 42 ◽  
pp. 234-235
Author(s):  
J. Curtis ◽  
K. S. Schwartz ◽  
R. P. Apkarian
Keyword(s):  
Anterior Pituitary ◽  
Lipid Droplets ◽  
Adrenocorticotropic Hormone ◽  
Unit Area ◽  
High Density Lipoproteins ◽  
Capillary Endothelium ◽  
Zona Fasciculata ◽  
Sem Study ◽  
Saline Vehicle ◽  
Scanning Electron

A scanning electron microscope (SEM) study was made of the effect of adrenocorticotropic hormone (ACTH) on the size and numbers of fenestrae/unit area in the capillary endothelium of the zona fasciculata (ZF) of the rat adrenal. The stimulatory effect of ACTH on cholesterol uptake via high density lipoproteins in the rat and evidence for the secretion of glucocorticoids by exocytosis of lipid droplets described by Rhodin suggest that endothelial change may accompany these transport phenomena.Twelve rats received two Dexamethasone (DEX) ip injections (25 μg DEX/100 g body wt.), the first at 8 PM and the second at 8 AM the next day, to inhibit the release of endogenous ACTH by the anterior pituitary. The animals were then divided into two groups. Six animals received only saline vehicle and six rats received ACTH (100 ng/100 g body wt.).

Early change of thyroid hormone concentration after 131I treatment in patients with solitary toxic adenoma

2002 ◽  
Vol 41 (04) ◽  
pp. 178-183 ◽  
Author(s):  
V. Fidler ◽  
K. Zaletel ◽  
S. Gaberšček ◽  
S. Hojker ◽  
E. Pirnat
Keyword(s):  
Thyroid Hormone ◽  
Antithyroid Drugs ◽  
Prospective Clinical Study ◽  
Hormone Concentration ◽  
Toxic Adenoma ◽  
Free Triiodothyronine ◽  
Thyroid Cells ◽  
131I Treatment ◽  
Before And After ◽  
Thyroid Hormone Concentration

Summary Aim: In spite of extensive use of 131I for treatment of hyperthyroidism, the results of early outcome are variable. In our prospective clinical study we tested whether 131I induced necrosis causing clinical aggravation of hyperthyroidism and increasing the free thyroid hormone concentration in the serum of patients with solitary toxic adenoma not pretreated with antithyroid drugs. Patients and methods: 30 consecutive patients were treated with 925 MBq 131I. Serum concentration of thyrotropin (TSH), free thyroxine (fT4), free triiodothyronine (fT3), thyroglobulin (Tg), and interleukin-6 (IL-6) were measured before and after application of 131I. Results: After application of 131I no clinical worsening was observed. FT4 and fT3 concentration did not change significantly within the first five days, whereas both of them significantly decreased after 12 days (p <0.0001). Slight and clinically irrelevant increase in the level of the two thyroid hormones was observed in 9 patients. Furthermore, we observed a prolonged increase in Tg concentration and a transient increase in IL-6 concentration. Conclusion: Neither evidence of any clinical aggravation of hyperthyroidism nor any significant increase in thyroid hormone concentration by 131I induced necrosis of thyroid cells was found. Therefore, the application of 131I may be considered as a safe and effective treatment for patients with hyperthyroidism due to toxic adenoma.

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