Dietary fish oil delays hypoxic skeletal muscle fatigue and enhances caffeine-stimulated contractile recovery in the rat in vivo hindlimb

Oxygen efficiency influences skeletal muscle contractile function during physiological hypoxia. Dietary fish oil, providing docosahexaenoic acid (DHA), reduces the oxygen cost of muscle contraction. This study used an autologous perfused rat hindlimb model to examine the effects of a fish oil diet on skeletal muscle fatigue during an acute hypoxic challenge. Male Wistar rats were fed a diet rich in saturated fat (SF), long-chain (LC) n-6 polyunsaturated fatty acids (n-6 PUFA), or LC n-3 PUFA DHA from fish oil (FO) (8 weeks). During anaesthetised and ventilated conditions (normoxia 21% O2 (SaO2–98%) and hypoxia 14% O2 (SaO2–89%)) the hindlimb was perfused at a constant flow and the gastrocnemius–plantaris–soleus muscle bundle was stimulated via sciatic nerve (2 Hz, 6–12V, 0.05 ms) to established fatigue. Caffeine (2.5, 5, 10 mM) was supplied to the contracting muscle bundle via the arterial cannula to assess force recovery. Hypoxia, independent of diet, attenuated maximal twitch tension (normoxia: 82 ± 8; hypoxia: 41 ± 2 g·g−1 tissue w.w.). However, rats fed FO sustained higher peak twitch tension compared with the SF and n-6 PUFA groups (P < 0.05), and the time to decline to 50% of maximum twitch tension was extended (SF: 546 ± 58; n-6 PUFA: 522 ± 58; FO: 792 ± 96 s; P < 0.05). In addition, caffeine-stimulated skeletal muscle contractile recovery was enhanced in the FO-fed animals (SF: 41 ± 3; n-6 PUFA: 40 ± 4; FO: 52 ± 7% recovery; P < 0.05). These results support a physiological role of DHA in skeletal muscle membranes when exposed to low-oxygen stress that is consistent with the attenuation of muscle fatigue under physiologically normoxic conditions.

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Long-chain n-3 DHA reduces the extent of skeletal muscle fatigue in the rat in vivo hindlimb model

British Journal Of Nutrition ◽

10.1017/s0007114513003449 ◽

2013 ◽

Vol 111 (6) ◽

pp. 996-1003 ◽

Cited By ~ 20

Author(s):

Gregory E. Peoples ◽

Peter L. McLennan

Keyword(s):

Skeletal Muscle ◽

Fish Oil ◽

Muscle Fatigue ◽

Muscle Membrane ◽

Mammalian Skeletal Muscle ◽

Skeletal Muscle Fatigue ◽

Arterial Blood ◽

Oxygen Efficiency ◽

Long Chain

Dietary fish oil modifies skeletal muscle membrane fatty acid composition and oxygen efficiency similar to changes in the myocardium. Oxygen efficiency is a key determinant of sustained force in mammalian skeletal muscle. Therefore, in the present study, we tested the effects of a fish-oil diet on skeletal muscle fatigue under the stress of contraction using the rat in vivo autologous perfused hindlimb model. For 8 weeks, male Wistar rats were fed a diet rich in saturated fat (SF), a diet rich in n-6 PUFA or a diet rich in long-chain (LC) n-3 PUFA DHA derived from fish oil. In anaesthetised, mechanically ventilated rats, with their hindlimbs perfused with arterial blood at a constant flow, the gastrocnemius–plantaris–soleus muscle bundle was stimulated via sciatic nerve (2 Hz, 6–12 V, 0·05 ms) to contract repetitively for 30 min. Rats fed the n-3 PUFA diet developed higher maximum twitch tension than those fed the SF and n-6 PUFA diets (P< 0·05) and sustained twitch tension through more repetitions before the tension declined to 50 % of the maximum twitch tension (P< 0·05). The n-3 PUFA group used less oxygen for tension developed and produced higher venous lactate concentrations with no difference in glycogen utilisation compared with the SF and n-6 PUFA groups. These results further support that incorporation of DHA into skeletal muscle membranes increases the efficiency of oxygen use over a range of contractile force and this is expressed as a higher sustained force and prolonged time to fatigue.

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Metabolic responses of canine gracilis muscle during contraction with partial ischemia

AJP Endocrinology and Metabolism ◽

10.1152/ajpendo.1996.270.3.e400 ◽

1996 ◽

Vol 270 (3) ◽

pp. E400-E406 ◽

Cited By ~ 6

Author(s):

J. A. Timmons ◽

S. M. Poucher ◽

D. Constantin-Teodosiu ◽

V. Worrall ◽

I. A. MacDonald ◽

...

Keyword(s):

Skeletal Muscle ◽

Muscle Fatigue ◽

Contractile Function ◽

Active Form ◽

Metabolic Effects ◽

Metabolic Responses ◽

Muscle Lactate ◽

Skeletal Muscle Fatigue ◽

Metabolic Characteristics ◽

Acetyl Group

The metabolic effects of partial ischemia on canine skeletal muscle were examined during 20 min of isometric contraction. A reduction in blood flow of approximately 75% resulted in an approximate 40% reduction in contractile function. Muscle lactate accumulation and phosphocreatine (PCr) hydrolysis were greater during ischemia, indicating a greater reliance on anaerobic ATP regeneration. Pyruvate dehydrogenase transformation to its active form (PDCa) during contraction was not affected by ischemia, such that PDCa did not appear to be a determinant of skeletal muscle fatigue. Acetylcarnitine concentration was greater during ischemic contraction and inversely correlated with PCr concentration (r = -0.79, P<0.01). Furthermore, acetylcarnitine accumulation and PCr degradation correlated with the degree of skeletal muscle fatigue (r = 0.56, P<0.05 and r = 0.70, P<0.01, respectively). Thus the greater the acetyl group oxidation, the lesser the contribution from anaerobic ATP provision and, subsequently, the smaller the degree of muscle fatigue observed. The metabolic characteristics of this model of ischemic muscle contraction are indistinguishable from the normal metabolic responses observed with increasing contractile intensity.

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Skeletal muscle fatigue does not affect shooting accuracy of handball players

Isokinetics and Exercise Science ◽

10.3233/ies-193178 ◽

2019 ◽

Vol 27 (4) ◽

pp. 253-259

Author(s):

Beyza Akyüz ◽

Pınar Arpınar Avşar ◽

Murat Bilge ◽

Gökhan Deliceoğlu ◽

Feza Korkusuz

Keyword(s):

Skeletal Muscle ◽

Muscle Fatigue ◽

Skeletal Muscle Fatigue

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Skeletal muscle fatigue in normal subjects and heart failure patients. Is there a common mechanism?

Acta Physiologica Scandinavica ◽

10.1046/j.1365-201x.1998.0343f.x ◽

1998 ◽

Vol 162 (3) ◽

pp. 215-228 ◽

Cited By ~ 19

Author(s):

P. K. LUNDE ◽

E. VERBURG ◽

N. K. VØLLESTAD ◽

O. M. SEJERSTED

Keyword(s):

Heart Failure ◽

Skeletal Muscle ◽

Muscle Fatigue ◽

Normal Subjects ◽

Common Mechanism ◽

Heart Failure Patients ◽

Skeletal Muscle Fatigue

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Mechanisms of Excitation-Contraction Coupling Relevant to Skeletal Muscle Fatigue

Advances in Experimental Medicine and Biology - Fatigue ◽

10.1007/978-1-4899-1016-5_4 ◽

1995 ◽

pp. 45-56 ◽

Cited By ~ 13

Author(s):

D. G. Stephenson ◽

G. D. Lamb ◽

G. M. M. Stephenson ◽

M. W. Fryer

Keyword(s):

Skeletal Muscle ◽

Muscle Fatigue ◽

Excitation Contraction Coupling ◽

Contraction Coupling ◽

Skeletal Muscle Fatigue

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Dysfunction of muscle contraction with impaired intracellular Ca2+ handling in skeletal muscle and the effect of exercise training in male db/db mice

Journal of Applied Physiology ◽

10.1152/japplphysiol.00048.2018 ◽

2019 ◽

Vol 126 (1) ◽

pp. 170-182 ◽

Cited By ~ 6

Author(s):

Hiroaki Eshima ◽

Yoshifumi Tamura ◽

Saori Kakehi ◽

Kyoko Nakamura ◽

Nagomi Kurebayashi ◽

...

Keyword(s):

Type 2 Diabetes ◽

Skeletal Muscle ◽

Exercise Training ◽

Muscle Contraction ◽

Contractile Function ◽

Contractile Force ◽

Contractile Dysfunction ◽

Muscle Contractile ◽

Type 2 Diabetic

Type 2 diabetes is characterized by reduced contractile force production and increased fatigability of skeletal muscle. While the maintenance of Ca2+ homeostasis during muscle contraction is a requisite for optimal contractile function, the mechanisms underlying muscle contractile dysfunction in type 2 diabetes are unclear. Here, we investigated skeletal muscle contractile force and Ca2+ flux during contraction and pharmacological stimulation in type 2 diabetic model mice ( db/db mice). Furthermore, we investigated the effect of treadmill exercise training on muscle contractile function. In male db/db mice, muscle contractile force and peak Ca2+ levels were both lower during tetanic stimulation of the fast-twitch muscles, while Ca2+ accumulation was higher after stimulation compared with control mice. While 6 wk of exercise training did not improve glucose tolerance, exercise did improve muscle contractile dysfunction, peak Ca2+ levels, and Ca2+ accumulation following stimulation in male db/db mice. These data suggest that dysfunctional Ca2+ flux may contribute to skeletal muscle contractile dysfunction in type 2 diabetes and that exercise training may be a promising therapeutic approach for dysfunctional skeletal muscle contraction. NEW & NOTEWORTHY The purpose of this study was to examine muscle contractile function and Ca2+ regulation as well as the effect of exercise training in skeletal muscle in obese diabetic mice ( db/db). We observed impairment of muscle contractile force and Ca2+ regulation in a male type 2 diabetic animal model. These dysfunctions in muscle were improved by 6 wk of exercise training.

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