Down-regulation of galactinol synthesis in oilseed Brassica napus leads to significant reduction of antinutritional oligosaccharidesThis paper is one of a selection of papers published in a Special Issue from the National Research Council of Canada – Plant Biotechnology Institute.

Botany ◽  
2009 ◽  
Vol 87 (6) ◽  
pp. 597-603 ◽  
Author(s):  
Cheryl Bock ◽  
Heather Ray ◽  
Fawzy Georges
Keyword(s):  
Brassica Napus ◽  
National Research Council ◽  
Plant Biotechnology ◽  
Antinutritional Factors ◽  
Mrna Levels ◽  
Brassica Napus L ◽  
Gene Encoding ◽  
Galactinol Synthase ◽  
Important Intermediate ◽  
Selection Of

The utility of defatted seed meal from many crops such as canola ( Brassica napus  L.) is limited by the presence of antinutritional factors, including sucrose galactosides, raffinose, and stachyose. Anaerobic breakdown of these sugars in the digestive tract of livestock is a major source of production of farm gases. In this report, the gene encoding galactinol synthase was isolated from B. napus and reintroduced into the same species in an antisense orientation to limit the production of galactinol, an important intermediate in the biosynthesis of raffinose and stachyose. This approach substantially reduced the accumulation of galactinol and stachyose in mature transgenic canola seed. Substantial changes in the mRNA levels of galactinol synthase and several sugar-related genes were also observed.

scholarly journals Proanthocyanidin biosynthesis in the seed coat of yellow-seeded, canola quality Brassica napus YN01-429 is constrained at the committed step catalyzed by dihydroflavonol 4-reductaseThis paper is one of a selection of papers published in a Special Issue from the National Research Council of Canada – Plant Biotechnology Institute.

Botany ◽  
2009 ◽  
Vol 87 (6) ◽  
pp. 616-625 ◽  
Author(s):  
Leonid Akhov ◽  
Paula Ashe ◽  
Yifang Tan ◽  
Raju Datla ◽  
Gopalan Selvaraj
Keyword(s):  
Brassica Napus ◽  
Seed Coat ◽  
National Research Council ◽  
Plant Biotechnology ◽  
Phenylpropanoid Metabolism ◽  
Brassica Napus L ◽  
Dihydroflavonol Reductase ◽  
Regulatory Circuits ◽  
Seed Coats ◽  
Canola Quality

The yellow seed characteristic in Brassica napus  L. is desirable because of its association with higher oil content and better quality of oil-extracted meal. YN01-429 is a yellow-seeded canola-quality germplasm developed in Canada arising from several years of research. Seed-coat pigmentation is due to oxidized proanthocyanidins (PA; condensed tannins) derived from phenylpropanoids and malonyl CoA. We found PA accumulation to be most robust in young seed coats (20 d post anthesis; dpa) of a related black-seeded line N89-53 and only very little PA in YN01-429, which also contained much less extractable phenolics. The flavonol content, however, did not show as great a difference between these two lines. Furthermore, sinapine, a product of the general phenylpropanoid metabolism, was present at comparable levels in the embryos of both lines. Dihydroflavonol reductase (DFR) activity that commits phenolics to PA synthesis was lower in YN01-429 seed coats. The results of Southern blot and in silico analyses were indicative of two copies of the DFR gene in B. napus. Both copies were functional in YN01-429, ruling out homeoallelic repression or silencing, but together they showed very low expression levels (17-fold fewer transcripts) relative to DFR activity in N89-53 seed coats. These results collectively suggest that YN01-429 differs in regulatory circuits that impact the PA synthesis branch much more than the flavonol synthesis branch in the seed coats and such circuits do not impinge upon general phenylpropanoid metabolism in the embryos.

Metabolic and hormonal processes associated with the induction of secondary dormancy in Brassica napus seedsThis paper is one of a selection of papers published in a Special Issue from the National Research Council of Canada – Plant Biotechnology Institute.

Botany ◽  
2009 ◽  
Vol 87 (6) ◽  
pp. 585-596 ◽  
Author(s):  
Houman Fei ◽  
Yurdagul Ferhatoglu ◽  
Edward Tsang ◽  
Daiqing Huang ◽  
Adrian J. Cutler
Keyword(s):  
Gene Expression ◽  
Polyethylene Glycol ◽  
Brassica Napus ◽  
National Research Council ◽  
Plant Biotechnology ◽  
Phenylpropanoid Metabolism ◽  
Brassica Napus L ◽  
Secondary Dormancy ◽  
Primary And Secondary Metabolism ◽  
Aba Insensitive

Polyethylene glycol treatment induces secondary seed dormancy in Brassica napus  L. cultivar ‘AC Excel’ (ACE), but not in ‘DH12075’ (DH). Gene expression, metabolite profiles, and hormone profiles were obtained from seeds of both cultivars following polyethylene glycol 8000 treatment. ACE seeds were more transcriptionally active: 28 genes were up-regulated in both cultivars and 10 and 158 genes were specifically up-regulated in DH and ACE, respectively. Nontargeted metabolite analyses combined with gene expression analyses showed significant differences in lipid, sugar, and phenylpropanoid metabolism between the cultivars. Abscisic acid (ABA) levels were higher and many ABA-inducible genes were expressed more in ACE. An association of ABA with secondary dormancy was supported by the observation that secondary dormancy was induced by polyethylene glycol 8000 in Arabidopsis wild-type seeds, but was reduced in ABA-deficient and ABA-insensitive mutants. Therefore, secondary dormancy appears to be realized through an active ABA-related mechanism that may involve changes in primary and secondary metabolism.

scholarly journals MORPHOLOGICAL CHARACTERISTICS OF BEE POLLEN OBTAINED FROM BRASSICA NAPUS L.

2016 ◽  
Vol 18 (2) ◽  
pp. 73-78
Author(s):  
N.M. Redina ◽  
L.O. Adamchuk ◽  
N.V. Nikolaieva ◽  
J. Brindza
Keyword(s):  
Brassica Napus ◽  
Local Population ◽  
Morphological Characteristics ◽  
Morphometric Measurements ◽  
Brassica Napus L ◽  
Pollen Collection ◽  
Bee Pollen ◽  
Total Pollen ◽  
Protein Feed ◽  
Selection Of

The purpose of this work was to research the morphological characteristics of monofloral bee pollen obtained from Brassica napus L. The formulated goals were: determine the period of bee pollen receiving; implement the selection of bee pollen samples during blooming period of species; establish the monoflorality ratio of the total pollen collection; explore the morphometric indicators of bee pollen by the parameters of pollen lump, shaping level and weight. Bee pollen collection was carried out in Skvyra district of Kyiv region from local population of Apis mellifera L. families. Morphometric measurements were performed in the Institute of Biodiversity Conservation and Biosafety, Slovak University of Agriculture in Nitra. Here were determined the amount of bee pollen per one day brought by one family: from  27.04 to 03.05.16 is 50.8 ± 2.25 g; from 04.05 to 06.05.16 – 100.0 ± 3.33 g; from 07.05 to 17.05.16 – 270.7 ± 11.79 g; from 18.05 to 22.05.16 – 100.5 ± 3.028 g; from 23.05 – 25.05.16 – 39.8 ± 2.74 g. Here were defined the morphological parameters of bee pollen lump from B. napus: length is in the range from 3.40 ± 0.061 mm to 3.66 ± 0.048 mm; width is in the range from 2.88 ± 0.059 mm to 3.26 ± 0.067 mm; weight is in the range from 8.65 ± 0.317 mg to 11.31 ± 0.241 mg; shaping level of bee pollen is in the range 4.62 – 4.96 points. The total monoflorality ratio constitutes from 79.94 ± 0.619 % to 98.00 ± 0.202 %. The obtained results confirmed that the activity of bee flying depends on the blooming period and intensity of the nectar secretion, on the collection of protein feed from B. napus. Pollen lumps from Brassica napus L. bee pollen were large, dense, regular shape without splits, which specified the possibility of its use for the producing on a commercial scale.  

scholarly journals Marker assisted selection of new high oleic and low linolenic winter oilseed rape (Brassica napus L.) inbred lines revealing good agricultural value

PLoS ONE ◽  
2020 ◽  
Vol 15 (6) ◽  
pp. e0233959 ◽  
Author(s):  
Stanisław Spasibionek ◽  
Katarzyna Mikołajczyk ◽  
Hanna Ćwiek–Kupczyńska ◽  
Teresa Piętka ◽  
Krystyna Krótka ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Oilseed Rape ◽  
Marker Assisted Selection ◽  
Inbred Lines ◽  
Winter Oilseed Rape ◽  
Brassica Napus L ◽  
High Oleic ◽  
Low Linolenic ◽  
Selection Of

scholarly journals Early in vitro selection of winter oilseed rape (Brassica napus L.) plants with the fertility restorer gene for CMS Shaan 2A via non-destructive molecular analysis of microspore-derived embryos

2019 ◽  
Vol 55 (No. 4) ◽  
pp. 162-165
Author(s):  
Miroslav Klíma ◽  
Eva Jozová ◽  
Irena Jelínková ◽  
Vratislav Kučera ◽  
Shengwu Hu ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Oilseed Rape ◽  
In Vitro Selection ◽  
Restorer Gene ◽  
Brassica Napus L ◽  
Rf Gene ◽  
Dh Lines ◽  
Non Destructive ◽  
Selection Of

Cotyledons of 201 microspore-derived oilseed rape (Brassica napus) embryos were analysed for the presence of the cytoplasmic male sterility Shaan 2A restorer gene using the primer pair 3F/4R. In total, 172 plants regenerated and were grown to the generative stage. Among phenotypically non-haploid flowering plants, there was a broad spectrum of different flower types from normally developed flowers to flowers with short anthers, stamens, or deformed flowers with atypically developed floral organs, often with traces of pollen. For this reason, only 115 typically fertile or sterile accessions were selected for further phenotype-genotype comparisons. In 14 plants (12.2%) the phenotype did not match the genotype: two plants were marked as fertile, but without the Rf gene. Vice versa, in 12 sterile plants the Rf gene was determined. Nevertheless, the two fertile plants did not produce any pods after self-pollination. In total, 47 doubled haploid (DH) lines with good seed yield, carrying the Rf gene were harvested.  

scholarly journals Study on Growth and Flowering Characteristics in the Spring Sowing for Selection of Rapeseed (Brassica napus L.) Varieties

2015 ◽  
Vol 28 (1) ◽  
pp. 111-118 ◽  
Author(s):  
Kwang-Soo Kim ◽  
Su-Ok Ha ◽  
Yong-Hwa Lee ◽  
Young-Seok Jang ◽  
In-Hu Choi
Keyword(s):  
Brassica Napus ◽  
Brassica Napus L ◽  
Spring Sowing ◽  
Selection Of

Early, non-destructive selection of microspore-derived embryo genotypes in oilseed rape (Brassica napus L.) by molecular markers and oil quality analysis

2007 ◽  
Vol 19 (3) ◽  
pp. 285-289 ◽  
Author(s):  
Ujjal Kumar Nath ◽  
Mohammed C. M. Iqbal ◽  
Christian Möllers
Keyword(s):  
Molecular Markers ◽  
Brassica Napus ◽  
Oilseed Rape ◽  
Oil Quality ◽  
Quality Analysis ◽  
Brassica Napus L ◽  
Non Destructive ◽  
Selection Of

scholarly journals Parental Selection of Hybrid Breeding Based On Maternal and Paternal Inheritance of Traits in Rapeseed (Brassica napus L.)

PLoS ONE ◽  
2014 ◽  
Vol 9 (7) ◽  
pp. e103165 ◽  
Author(s):  
Nailin Xing ◽  
Chuchuan Fan ◽  
Yongming Zhou
Keyword(s):  
Brassica Napus ◽  
Hybrid Breeding ◽  
Paternal Inheritance ◽  
Brassica Napus L ◽  
Parental Selection ◽  
Selection Of

Selection of Essential Oils Inhibiting Germination and Initial Growth of Rapeseed (Brassica napus L.)

2010 ◽  
Vol 30 (3) ◽  
pp. 199-205 ◽  
Author(s):  
Sung-Hwan Choi ◽  
Kee-Woong Park ◽  
Young-Geol Sohn ◽  
Jae-Young An ◽  
Jeung-Joo Lee
Keyword(s):  
Brassica Napus ◽  
Essential Oils ◽  
Initial Growth ◽  
Brassica Napus L ◽  
Selection Of
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