TISSUE CULTURE OF PINUS MONTICOLA ON A CHEMICALLY DEFINED MEDIUM

1967 ◽  
Vol 45 (10) ◽  
pp. 1783-1787 ◽  
Author(s):  
Alan E. Harvey
Keyword(s):  
Calcium Nitrate ◽  
Potassium Phosphate ◽  
Defined Medium ◽  
Manganese Sulfate ◽  
Tissue Cultures ◽  
Chemically Defined Medium ◽  
Pinus Monticola ◽  
White Pine ◽  
Potassium Phosphate Monobasic ◽  
Western White Pine

Procedures for obtaining and maintaining axenic tissue cultures of Pinus monticola are described. Western white pine tissue was cultured on a chemically defined medium containing calcium nitrate, magnesium sulfate, potassium phosphate (monobasic), ammonium sulfate, ferric sulfate, manganese sulfate, glucose, and one of three auxins, IAA, NAA, or 2,4-D. Addition of several B vitamins, amino acids, and kinetin increased growth on these media but were not required.

Growth of the rust fungus Cronartium ribicola in tissue cultures of Pinus monticola

1969 ◽  
Vol 47 (5) ◽  
pp. 663-666 ◽  
Author(s):  
A. E. Harvey ◽  
J. L. Grasham
Keyword(s):  
Callus Tissue ◽  
Rust Fungus ◽  
Calcium Nitrate ◽  
Potassium Phosphate ◽  
Naphthaleneacetic Acid ◽  
Manganese Sulfate ◽  
Cronartium Ribicola ◽  
Pinus Monticola ◽  
White Pine ◽  
Western White Pine

Cambial explants of western white pine, Pinus monticola, infected with Cronartium ribicola, were cultured on a medium containing glucose, calcium nitrate, magnesium sulfate, potassium phosphate (monobasic), ammonium sulfate, ferric sulfate, manganese sulfate, and one of three auxins: indoleacetic acid (IAA), naphthaleneacetic acid (NAA), or 2,4-(dichlorophenyoxy)acetic acid (2,4-D).Invasion of newly formed callus tissue by the rust fungus was generally slow. Dense, feltlike aerial mycelia were frequently produced. Intercellular mycelia and intracellular haustoria were typical, and direct attachments to aerial mycelia were observed. Occasionally, mycelia invaded the medium, but did not become independent of the host tissue. Typical pycnia- and aecia-like sori were produced in many cultures. Aecia-like sori formed immediately after maturation of pycnia, but did not produce spores.

In vivo verification of Cronartium ribicola propagated on tissue cultures of Pinus monticola

1970 ◽  
Vol 48 (7) ◽  
pp. 1429-1430 ◽  
Author(s):  
A. E. Harvey ◽  
J. L. Grasham
Keyword(s):  
Infected Tissue ◽  
Cronartium Ribicola ◽  
Tissue Cultures ◽  
Pinus Monticola ◽  
White Pine ◽  
Rust Infection ◽  
Western White Pine ◽  
Host Tissues

Inoculations of western white pine seedling stems with rust-infected tissue cultures produced one successful rust infection after 5 months. The infection was typical of this rust (Cronartium ribicola J. C. Fisch. ex Rabenh.) and the presence of haustoria was confirmed. Infected cortex tissue from this seedling was used to reestablish the isolate on host tissues grown in vitro.

Growth of Cronartium ribicola in the absence of physical contact with its host

1970 ◽  
Vol 48 (1) ◽  
pp. 71-73 ◽  
Author(s):  
A. E. Harvey ◽  
J. L. Grasham
Keyword(s):  
Host Tissue ◽  
Physical Contact ◽  
Cronartium Ribicola ◽  
Tissue Cultures ◽  
Pinus Monticola ◽  
White Pine ◽  
Western White Pine ◽  
Agar Media ◽  
Healthy Host

Rust mycelia from tissue cultures infected with Cronartium ribicola J. C. Fisch. ex Rabenh. were grown on cellophane membranes in the absence of physical contact with host tissue. These membranes were in contact with agar media containing imbedded, actively growing western white pine (Pinus monticola Dougl.) tissue cultures. Identities of these cultures were verified by reestablishing infections in healthy host tissue cultures.

Inoculation of western white pine tissue cultures with basidiospores of Cronartium ribicola

1970 ◽  
Vol 48 (7) ◽  
pp. 1309-1311 ◽  
Author(s):  
A. E. Harvey ◽  
J. L. Grasham
Keyword(s):  
Cronartium Ribicola ◽  
Tissue Cultures ◽  
Pinus Monticola ◽  
White Pine ◽  
Western White Pine ◽  
Direct Inoculation

Rust infections were established in healthy, undifferentiated tissue cultures of Pinus monticola Dougl. by direct inoculation with germinating basidiospores of Cronartium ribicola J. C. Fisch. ex Rabenh. Infections were verified by confirming the presence of typical rust haustoria and extensive intracellular ramification by the mononucleate rust mycelium.

Procedures and media for obtaining tissue cultures of 12 conifer species

1969 ◽  
Vol 47 (4) ◽  
pp. 547-549 ◽  
Author(s):  
A. E. Harvey ◽  
J. L. Grasham
Keyword(s):  
Pinus Ponderosa ◽  
Calcium Nitrate ◽  
Potassium Phosphate ◽  
Basal Medium ◽  
Thuja Plicata ◽  
Individual Species ◽  
Naphthaleneacetic Acid ◽  
Manganese Sulfate ◽  
Tissue Cultures ◽  
Larix Occidentalis

Procedures for obtaining axenic tissue cultures of Abies grandis, Larix occidentalis, Picea abies, Picea engelmanni, Picea pungens, Pinus albicaulis, Pinus contorta, Pinus flexilis, Pinus nigra, Pinus ponderosa, Pseudotsuga menziesii, and Thuja plicata are described. All species were cultured on a basal medium containing calcium nitrate, magnesium sulphate, potassium phosphate (monobasic), ammonium sulfate, ferric sulfate, manganese sulfate, glucose, 0.8% Bacto agar, and one of three auxins (indoleacetic acid (IAA), naphthaleneacetic acid (NAA), or 2,4-dichlorophenoxyacetic acid (2,4-D)) at various concentrations. Growth of most species was further enhanced by the addition of several vitamins and amino acids. These species were relatively specific in their auxin requirements. The type of sterilant (H2O2, NaOCl) and manipulation required in the preparatory procedures were also specific for individual species.

Western white pine development in relation to biophysical characteristics across different spatial scales in the Coeur d'Alene River basin in northern Idaho, U.S.A.

2002 ◽  
Vol 32 (7) ◽  
pp. 1109-1125 ◽  
Author(s):  
Theresa B Jain ◽  
Russell T Graham ◽  
Penelope Morgan
Keyword(s):  
River Basin ◽  
Spatial Scales ◽  
Pinus Monticola ◽  
White Pine ◽  
Basal Diameter ◽  
Landscape Characteristics ◽  
Coeur D'alene ◽  
Biophysical Factors ◽  
Western White Pine ◽  
Northern Idaho

Many studies have assessed tree development beneath canopies in forest ecosystems, but results are seldom placed within the context of broad-scale biophysical factors. Mapped landscape characteristics for three watersheds, located within the Coeur d'Alene River basin in northern Idaho, were integrated to create a spatial hierarchy reflecting biophysical factors that influence western white pine (Pinus monticola Dougl. ex D. Don) development under a range of canopy openings. The hierarchy included canopy opening, landtype, geological feature, and weathering. Interactions and individual-scale contributions were identified using stepwise log–linear regression. The resulting models explained 68% of the variation for estimating western white pine basal diameter and 64% for estimating height. Interactions among spatial scales explained up to 13% of this variation and better described vegetation response than any single spatial scale. A hierarchical approach based on biophysical attributes is an excellent method for studying plant and environment interactions.

scholarly journals The Transferability of Western White Pine to and within British Columbia Based on Early Survival, Environmental Damage, and Juvenile Height

1999 ◽  
Vol 14 (1) ◽  
pp. 41-47 ◽  
Author(s):  
M. D. Meagher ◽  
R. S. Hunt
Keyword(s):  
Root Rot ◽  
Source Parameters ◽  
Test Series ◽  
Environmental Damage ◽  
Pinus Monticola ◽  
White Pine ◽  
Spring Frost ◽  
Provenance Test ◽  
Late Spring Frost ◽  
Western White Pine

Abstract Survival, environmental damage, and juvenile height of 27 provenances of western white pine (Pinus monticola) in three plantation series were analyzed after 5 to 13 yr on site to assist in evaluating seed-transfer practices. Survival averaged 79.2% on nine sites in the "root-rot" series and 84.1% on six sites in the "provenance-test" series. Trends of survival on seed-source parameters differed between series, generally increasing with both elevation and latitude in the root-rot series, while generally decreasing with latitude in the provenance-test series. Analysis by seed zone (coast or interior) and plantation region (coast, southern interior, or northern interior) showed that coastal sources on interior sites caused much of these anomalies. Substantial environmental damage was found only on sites near to or beyond the species' northern limit. Differences in the trend of damage with source parameters were found between the test series: interior sources were damaged less than coastal sources on two root-rot sites, whereas interior sources were damaged more heavily than coastal sources on the provenance-test site exhibiting substantial damage. Damage increased with increasing provenance latitude and elevation in the root-rot series, while it dropped with increasing elevation in the "provenance-test" series. In general, taller seedlings in taller provenances were damaged. Coastal seed should not be used on interior sites, but transfer of seed from the BC interior to the BC coast seems safe. We recommend that the present limits for latitudinal transfer be doubled, except where late-spring-frost risk is high, and that elevational transfer of seeds for the interior zone be reduced by about half West. J. Appl. For. 14(1)41-47.

scholarly journals White Pine Blister Rust in British Columbia III. Effects on the Gene Pool of Western White Pine

1985 ◽  
Vol 61 (6) ◽  
pp. 484-488 ◽  
Author(s):  
R. S. Hunt ◽  
J. F. Manville ◽  
E. von Rudloff ◽  
M. S. Lapp
Keyword(s):  
Pacific Northwest ◽  
Gene Pool ◽  
Resistance Mechanisms ◽  
Geographic Range ◽  
White Pine Blister Rust ◽  
Pinus Monticola ◽  
White Pine ◽  
Blister Rust ◽  
The Pacific ◽  
Western White Pine

Cluster analyses of relative terpene abundance in foliage of western white pine (Pinus monticola Dougl.) trees from throughout the Pacific Northwest geographic range of the species were produced. Terpene patterns were randomly distributed among populations; no geographic or site trends were evident. Although blister rust is devastating to stands, the gene pool is widely distributed and may well be preserved without establishing gene banks.About 40-50 trees selected at random would yield offspring with nearly all possible terpene patterns characteristic of the species and would thus constitute a broad genetic base. Therefore seed orchards do not necessarily need to be composed of many individuals, rather, they should contain highly selected individuals with multiple desirable traits including multiple blister rust resistance mechanisms. Key words: terpenes, dendrogram

Microculture of western white pine (Pinus monticola) by induction of shoots on bud explants from 1- to 7-year-old trees

1996 ◽  
Vol 16 (4) ◽  
pp. 447-451 ◽  
Author(s):  
M. S. Lapp ◽  
J. Malinek ◽  
M. Coffey
Keyword(s):  
Pinus Monticola ◽  
White Pine ◽  
Western White Pine ◽  
Old Trees
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