In vitro germination of black walnut pollen

Black walnut pollen was germinated on liquid and agar-based media containing 20% sucrose, a requirement for germination and normal tube development. Boron (100 ppm boric acid) added to this medium slightly enhanced germination. Germination occurred only in dense (150+ grains/mm2) populations of pollen on the agar medium. This population effect was not overcome by the addition of Ca2+ to the medium, but germination in the sparse areas (< 30 grains/mm2) was occasionally promoted by high boron concentrations (600 ppm boric acid). Wide tree-to-tree variation in percentage of germination was observed.Vacuum-drying periods, ranging from 5 to 30 min, resulted in a gradual reduction of viability. Pollen was stored at −30C and −196C for 3 months without major loss of viability.

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Effects of Sucrose, Boric Acid, pH, and Incubation Time on in Vitro Germination of Pollen and Tube Growth of Chinese fir (Cunnighamial lanceolata L.)

Forests ◽

10.3390/f10020102 ◽

2019 ◽

Vol 10 (2) ◽

pp. 102 ◽

Cited By ~ 3

Author(s):

Seif Fragallah ◽

Sizu Lin ◽

Nuo Li ◽

Elly Ligate ◽

Yu Chen

Keyword(s):

Boric Acid ◽

Pollen Germination ◽

Tree Breeding ◽

Chinese Fir ◽

Tube Growth ◽

In Vitro Germination ◽

Acid Ph ◽

Novel Approach ◽

The Media

In vitro pollen germination provides a novel approach and strategy to accelerate genetic improvement of tree breeding. Studies about pollen germination and tube growth of Chinese fir are limited. Therefore, this study aimed to investigate the effects of sucrose, boric acid, pH, and time of incubation on pollen germination and tube growth. Pollen from 9 clones were selected. In vitro germination was performed in basic media as control, and in different concentrations of sucrose (0, 10 and 15%), boric acid (0.01, 0.1 and 0.2%), and pH levels (4.5, 5 and 7). Pollen germination rates and tube growth were recorded periodically at 1, 12, 24, and 48 h. The results showed that sucrose imposes significant effects on pollen germination and tube growth. The effects are most obvious at concentration of 15%. Boric acid significantly promoted germination and tube growth. The promotion was most notable in lower concentration of 0.01%. The media adjusted to pH 7.0 boosted the germination and pollen tube growth. The optimum time of incubation was 24 and 48 h for pollen germination and tube growth, respectively. Sucrose, pH, and time of incubation were positively correlated, whereas boric acid negatively correlated with pollen germination and tube growth. This study provided experimental evidences for selecting viable pollens for Chinese fir breeding.

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Viability and in Vitro Germination of Johnsongrass (Sorghum Halepense) Pollen

Weed Technology ◽

10.1614/wt-05-171.1 ◽

2007 ◽

Vol 21 (1) ◽

pp. 23-29 ◽

Cited By ~ 4

Author(s):

Ian C. Burke ◽

John W. Wilcut ◽

Nina S. Allen

Keyword(s):

Suspension Culture ◽

Boric Acid ◽

Pollen Germination ◽

Pollen Viability ◽

Calcium Nitrate ◽

Medium Composition ◽

In Vitro Tests ◽

In Vitro Germination ◽

Agar Culture

A high proportion of viable pollen grains must germinate to study the physiology of pollen growth to reduce the confounding effects of environmental influences on pollen germination. The objectives of this study were to evaluate the nuclear state and develop a suitable medium and culture method for in vitro germination of johnsongrass pollen. Johnsongrass pollen was trinucleate, and in vitro tests for pollen viability using Alexander's stain and a fluorochromatic reaction method (FCR) indicated johnsongrass pollen was viable (92.6 to 98.4%). A factorial treatment arrangement of four concentrations of sucrose, two concentrations of boric acid, and two concentrations of calcium nitrate were used to determine the optimum pollen-germination medium composition in suspension culture, agar culture, and cellophane membrane culture. Germination was highest in a suspension culture with a medium containing 0.3 M sucrose, 2.4 mM boric acid, and 3 mM calcium nitrate. Pollen germination using this medium was 78.9% when anthers were harvested just before anthesis.

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Optimising storage and in vitro germination of Eucalyptus pollen

Australian Journal of Botany ◽

10.1071/bt05194 ◽

2007 ◽

Vol 55 (1) ◽

pp. 83 ◽

Cited By ~ 5

Author(s):

Tasmien N. Horsley ◽

Steven D. Johnson ◽

Terrence K. Stanger

Keyword(s):

Boric Acid ◽

Pollen Germination ◽

Storage Temperature ◽

Sucrose Solution ◽

Germination Rate ◽

Pollen Viability ◽

Eucalyptus Grandis ◽

In Vitro Germination ◽

Sucrose Solutions

The best sucrose solution for maximum in vitro germination of Eucalyptus pollen was investigated in order to evaluate pollen germination rate as an indicator of pollen viability. In vitro germination of both freshly collected and 1-year-old pollen (stored at 4°C) of Eucalyptus grandis, E. smithii, E. nitens, E. dunnii and E. macarthurii was carried out in 0, 10, 20, 30, 40 and 50% (w/v) sucrose solutions, either with (0.15 mg L–1) or without boric acid. Similar trends were obtained for both fresh and 1-year-old pollen, with all species responding most favourably to 30% (w/v) sucrose and 0.15 mg L–1 boric acid. When an optimal in vitro germination medium had been established, the viabilities (%germination) of E. smithii, E. nitens and E. grandis pollen, stored at room (25°C), fridge (4°C), freezer (–10°C) and liquid nitrogen (–196°C) temperatures, were compared. For all tested species, germination declined as storage temperature increased, and by 8 months, the highest survival was obtained with cryostored pollen.

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In vitro germination protocol for the propagation of the endangered butternut tree (Juglans cinerea L.)

Native Plants Journal ◽

10.3368/npj.20.2.117 ◽

2019 ◽

Vol 20 (2) ◽

pp. 117-122 ◽

Cited By ~ 1

Author(s):

Martin Williams ◽

Kathleen Forbes ◽

Charlene Williams ◽

Tannis Beardmore

Keyword(s):

In Vitro Germination ◽

Juglans Cinerea

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IN VITRO GERMINATION AND POLLEN TUBE GROWTH OF MAIZE (ZEA MAYS L.) POLLEN. X. POLLEN SOURCE GENOTYPE AND GIBBERELLIN A3INTERACTIONS*

Acta Botanica Neerlandica ◽

10.1111/j.1438-8677.1982.tb01596.x ◽

1982 ◽

Vol 31 (1-2) ◽

pp. 105-111 ◽

Cited By ~ 3

Author(s):

P. L. Pfahler ◽

M. Wilcox ◽

D. L. Mulcahy ◽

D. A. Knauft

Keyword(s):

Zea Mays ◽

Pollen Tube ◽

Pollen Tube Growth ◽

Zea Mays L ◽

Tube Growth ◽

In Vitro Germination

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Low temperature storage and in vitro germination of cherimoya (Annona cherimola Mill.) pollen

Scientia Horticulturae ◽

10.1016/j.scienta.2005.12.003 ◽

2006 ◽

Vol 108 (1) ◽

pp. 91-94 ◽

Cited By ~ 26

Author(s):

J. Lora ◽

M.A. Pérez de Oteyza ◽

P. Fuentetaja ◽

J.I. Hormaza

Keyword(s):

Low Temperature ◽

In Vitro Germination ◽

Low Temperature Storage ◽

Annona Cherimola ◽

Temperature Storage

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In vitro germination and transient GFP expression of American chestnut (Castanea dentata) pollen

Plant Cell Reports ◽

10.1007/s00299-005-0088-z ◽

2005 ◽

Vol 25 (5) ◽

pp. 450-456 ◽

Cited By ~ 9

Author(s):

Danilo D. Fernando ◽

Javonna L. Richards ◽

Julie R. Kikkert

Keyword(s):

Castanea Dentata ◽

American Chestnut ◽

In Vitro Germination ◽

Gfp Expression

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In Vitro Germination of Several South African Summer Rainfall Disa (Orchidaceae) Species: Is Seed Testa Structure a Function of Habitat and a Determinant of Germinability?

Systematics and Geography of Plants ◽

10.2307/3668704 ◽

2001 ◽

Vol 71 (2) ◽

pp. 597 ◽

Cited By ~ 9

Author(s):

Dave I. Thompson ◽

Trevor J. Edwards ◽

Johannes van Staden

Keyword(s):

South African ◽

Summer Rainfall ◽

In Vitro Germination ◽

Seed Testa

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Control of browning during seed germination of Meizotropis pellita: a rare and an endangered plant

Research Journal of Biotechnology ◽

10.25303/168rjbt11821 ◽

2021 ◽

Vol 16 (8) ◽

pp. 118-120

Author(s):

Renu Chandola ◽

Anshulika Upadhyaya

Keyword(s):

Seed Germination ◽

Culture Media ◽

Light Condition ◽

Endangered Plant ◽

In Vitro Germination ◽

Light Conditions ◽

Low Intensity ◽

Browning Reaction ◽

Threatened Plant Species

Meizotropis pellita is a rare, endangered and threatened plant species found in Patwadanger forest. In nature this species grows from rootstock in precise and sensitive habitats. However, phenolic compounds provoke browning reaction which is responsible for lethal browning in plant tissue culture and hindering in vitro germination. The aim of this study was to minimize the effect of phenolic compound during seed germination. We evaluate different antibrowning treatments in Meizotropis pellita seeds. The seeds were pretreated in Polyvinylpyrrolidone and then treated seeds were cultured on MS media and sterilized seeds were directly inoculated on PVP containing MS media. These culture media incubate under dark and light conditions at different durations respectively. Our results showed that addition of antibrowning agent, PVP inhibit onset of browning. Dark and light condition also played a crucial role on onset of browning. Delayed and low intensity of browning were observed in case of seeds that were inoculated on MS media containing PVP and were kept in dark. The present research offers a positive in vitro seed germination protocol for Meizotropis pellita conservation.

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