Investigations of growth-promoting factors in conditioned soybean root cells and in the liquid medium in which they grow: cytokinin-like compounds

1974 ◽  
Vol 52 (12) ◽  
pp. 2459-2463 ◽  
Author(s):  
P. A. Sargent ◽  
J. King
Keyword(s):  
Amino Acids ◽  
Glycine Max ◽  
Liquid Medium ◽  
Cell Suspension ◽  
Cell Suspension Cultures ◽  
Plant Tissues ◽  
Root Cells ◽  
Soybean Cotyledon ◽  
Glycine Max L ◽  
Growth Promoting

By using the soybean cotyledon callus bioassay for cytokinin-like compounds and two methods of extraction of cytokinins from plant tissues, the presence of growth-active compounds of a purine nature has been detected in soybean (Glycine max. L. cv. Mandarin) root cell suspension cultures growing in a medium containing NH4+. These compounds can be added to residual NH4+, glutamine, and an adequate supply of amino acids as conditioning substances in these cultures.

Investigations of growth-promoting factors in conditioned soybean root cells and in the liquid medium in which they grow: ammonium, glutamine, and amino acids

1974 ◽  
Vol 52 (7) ◽  
pp. 1747-1755 ◽  
Author(s):  
P. A. Sargent ◽  
J. King
Keyword(s):  
Amino Acids ◽  
Dry Weight ◽  
Liquid Media ◽  
Root Cells ◽  
Nitrogenous Compounds ◽  
Soybean Root ◽  
Haplopappus Gracilis ◽  
Glycine Max L ◽  
Growth Promoting ◽  
Cells Cultured

Cells cultured in sterile, liquid media from a number of Phaseolus spp., soybean cotyledons, shoots, and roots and from rice explants grew, in terms of dry-weight increase, much better in the presence of NH4+ and NO3− as sources of nitrogen than with NO3− alone. Other cultures tested, including other legumes, either did not respond positively to added NH4+ or, as in the case of Haplopappus gracilis cells, grew better in its absence.Earlier it had been shown that soybean (Glycine max. L. cv. Mandarin) root cells grew better in the presence of NH4+ than in its absence and that 'conditioning' substances were produced by cells and excreted into the medium between about the 15th and 35th h of incubation. These observations and those above with other cell cultures led to the initiation of an investigation of why some cells respond to NH4+ while others do not.This investigation has so far taken the form of an analysis of nitrogenous compounds in soybean root cells and in the NH4+-containing medium in which they were grown during 120 h of incubation and especially after 24 h of incubation, the time of maximum production of growth-enhancing ability in both cells and medium.Growth enhancement can be accounted for, apparently, by the occurrence of residual NH4+ in conditioned medium and by the presumed occurrence of NH4+ in cells. However, glutamine and its derivatives are implicated in the conditioning process.

Identification of an UDP-glucose: Flavonol 3-O-glucosyl-transferase from cell suspension cultures of soybean (Glycine max L.)

Planta ◽  
1977 ◽  
Vol 136 (1) ◽  
pp. 53-59 ◽  
Author(s):  
J. E. Poulton ◽  
Margit Kauer
Keyword(s):  
Glycine Max ◽  
Cell Suspension ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Glycine Max L

Metabolism of DDT and Kelthane in cell suspension cultures of parsley (Petroselinum hortense, Hoffm.) and soybean (Glycine max L.)

Planta ◽  
1977 ◽  
Vol 133 (3) ◽  
pp. 315-320 ◽  
Author(s):  
D. Scheel ◽  
H. Sandermann
Keyword(s):  
Glycine Max ◽  
Cell Suspension ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Glycine Max L

Comparison of Dry Matter Synthesis and Photosynthetic Characteristics of Chlorophyllous and Non-chlorophyllous Cell Suspension Cultures of Soybean (Glycine max. L.)

1982 ◽  
Vol 105 (5) ◽  
pp. 445-456 ◽  
Author(s):  
Yvonne M. de Klerk-Kiebert ◽  
Tarcies J.A. Kneppers ◽  
Peter A.H.M. Barker ◽  
Herman H. Schalk
Keyword(s):  
Glycine Max ◽  
Cell Suspension ◽  
Dry Matter ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Photosynthetic Characteristics ◽  
Glycine Max L

Amino acid transport systems of cultured soybean root cells

1975 ◽  
Vol 53 (18) ◽  
pp. 2088-2091 ◽  
Author(s):  
J. King ◽  
Rozina Hirji
Keyword(s):  
Amino Acids ◽  
Glycine Max ◽  
Amino Acid ◽  
Amino Acid Transport ◽  
Transport Systems ◽  
Acid Transport ◽  
Root Cells ◽  
Soybean Root ◽  
Neutral Amino Acids ◽  
Glycine Max L

The uptake of 1 μM14C-labelled arginine, glutamate, and alanine by cultured soybean (Glycine max L. cv. Mandarin) root cells was followed for periods up to 4 min at pH 5.5 in the presence of a 10 μM concentration of other amino compounds. From the degree of competition between 14C-labelled and unlabelled amino acids a number of uptake systems for basic, acidic, and neutral amino acids were identified, and a number of problems associated with amino acid transport in soybean cells were uncovered.

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