Vegetative propagation of sandalwood plants through tissue culture

1978 ◽  
Vol 56 (9) ◽  
pp. 1153-1156 ◽  
Author(s):  
P. S. Rao ◽  
V. A. Bapat
Keyword(s):  
Hormonal Treatment ◽  
Santalum Album ◽  
Nutrient Media ◽  
Bud Formation ◽  
Shoot Bud ◽  
Basal Media ◽  
Adult Plants ◽  
Stem Segments ◽  
Better Than

Hypocotyl segments excised from seedlings of Santalum album (sandalwood tree) grown in vitro were cultured aseptically on various nutrient media. Regeneration of shoot buds on explants was observed on basal media supplemented with auxins like IAA, IBA, NAA, or NOA. Shoot bud formation was completely suppressed on 2,4-D or pCPA medium. Cytokinins stimulated and enhanced shoot bud formation better than auxins. Excised stem segments from adult plants did not respond to any hormonal treatment. The importance of this method in the propagation of the sandalwood tree is emphasized.

Influence of Cytokinins on Rhizome Mediated Growth and Morphogenesis of an Endangered Medicinal Orchid Geodorum densiflorum (Lam.) Schltr.

2020 ◽  
Vol 30 (1) ◽  
pp. 65-75
Author(s):  
Soumi Bhattacharyya ◽  
Nirmalya Banerjee
Keyword(s):  
Seed Germination ◽  
Growth And Development ◽  
Large Scale ◽  
Nutrient Media ◽  
Asymbiotic Seed Germination ◽  
Shoot Bud ◽  
Basal Media ◽  
Medicinal Orchid ◽  
Later Phase

Effects of different nutrient media, vitamins and peptone on in vitro asymbiotic seed germination of Geodorum densiflorum (Lam.) Schltr. were studied. In vitro developed rhizomes were used to determine the influence of cytokinins on growth and morphogenesis. Seed germination and survival rate of protocorms were highest in MS medium compared to other basal media. The protocorms raised through seed germination directly proliferated into rhizomes in later phase of growth and development. Nodal portions of rhizomes further exhibited growth through formation of direct and callus mediated protocorm like bodies. In general, three types of rhizome movements were noticed; viz. positively geotropic, negatively geotropic and diagravitropic movements. In the PGR free control and in the presence of NAA alone rhizomes exhibited positively geotropic movements. On the contrary, presence of cytokinin either alone or in combination with NAA exhibited diagravitropic movements. Application of TDZ completely suppressed the positively geotropic movement and enhanced the frequency of negatively geotropic movement of rhizomes followed by shoot bud formation. BAP was most effective cytokinin for large scale rhizome mediated multiplication of Geodorum densiflorum and TDZ could be effectively employed for rapid leafy shoot regeneration. Plant Tissue Cult. & Biotech. 30(1): 65-75, 2020 (June)

scholarly journals Clonal Propagation In Vitro of Paphiopedilum Hybrids from Adult Plants

HortScience ◽  
2019 ◽  
Vol 54 (9) ◽  
pp. 1565-1569
Author(s):  
Vi Nguyen Tuong Do ◽  
Shan-Te Hsu ◽  
Yung-I Lee
Keyword(s):  
Clonal Propagation ◽  
Shoot Multiplication ◽  
Shoot Tip ◽  
Seasonal Effect ◽  
Optimal Timing ◽  
Axillary Shoot ◽  
Shoot Bud ◽  
Shoot Tip Explants ◽  
Adult Plants

The aim of this study was to develop an efficient protocol for shoot tip culture from adult plants of Paphiopedilum Pfitzer. A considerable seasonal effect on explant collection was observed in the aseptic cultures established from adult plants, including the survival and microbial contamination of explants. The shoot tip explants excised from adult plants in February and May showed higher survival and had less contamination than those explants excised in August and November. Moreover, the season of explant collection also affected the subsequent shoot forming capacity and multiplication of axillary buds. In Paphiopedilum ‘In-Charm Silver Bell’, higher shoot forming capacity was observed in February and May, whereas higher shoot multiplication was observed only in February. In Paphiopedilum ‘Hsinying Maudiae Leopard’, both February and May were optimal timing for shoot forming capacity and multiplication. We also demonstrated the effectiveness of transcinnamic acid (tCA), an antiauxin chemical in diminishing the apical dominance of shoot tip explant and thus improving the axillary bud outgrowth. In P. ‘In-Charm Silver Bell’, the addition of 100 μM tCA plus 13.3 μM 6-benzylaminopurine (BA) for 1 month promoted axillary shoot bud formation from shoot tip explants as compared with the control.

scholarly journals High Frequency Regeneration of Plantlets from Immature Male Floral Explants of Musa paradisica cv. Puttabale - AB Genome

2011 ◽  
Vol 21 (2) ◽  
pp. 199-205 ◽  
Author(s):  
K. Girish Kumar ◽  
V. Krishna ◽  
Venkatesh Venkatesh ◽  
K. Pradeep
Keyword(s):  
Callus Induction ◽  
High Frequency ◽  
In Vitro Regeneration ◽  
Floral Part ◽  
Bud Formation ◽  
Shoot Bud ◽  
Immature Male ◽  
Floral Explants ◽  
High Frequency Induction

High frequency in vitro regeneration for mass multiplication from immature male floral explants of Musa paradisica cv. Puttabale on MS supplemented with adenine sulfate (160 mg/l), tyrosine (100 mg/l), sucrose (40 g/l) and gelled with 0.8 g/l agar was attempted.  For callus induction the combinations of 2, 4-D and BAP were tested at 1.0 - 10.0 mg/l and 0.5 - 5.0 mg/l, respectively. For shoot bud formation combinations of BAP and TDZ were also tested at 1.0 - 5.0 mg/l and 0.1 - 0.5 mg/l, respectively.  Luxuriant proliferation and high frequency induction (97.0%) of  callus  was  noticed from the  accessory  floral  part  of  the  explant  at 7.0 mg/l 2, 4-D and 1.0 mg/l BAP, later it preceded towards the gynoecium. Interaction of BAP (2.0 – 5.0 mg/l) and TDZ (0.2 - 0.5 mg/l) would provoke high frequency shoot bud differentiation from the floral calli and a mean of 29.40 ± 6.10 shootlets per callus was obtained at 4 mg/l BAP and 0.4 mg/l TDZ. Rooting of the microshoots was achieved on MS containing 0.6 mg/l NAA and 0.2% activated charcoal.     Key words: Musa pardisica, Puttabale, Regeneration, Male floral explants.   D. O. I. 10.3329/ptcb.v21i2.10243   Plant Tissue Cult. & Biotech. 21(2): 199-205, 2011 (December)

Enhanced in vitro caulogenesis and quantitative estimation of aloin through HPLC in Aloe vera

2013 ◽  
Vol 61 (1-4) ◽  
pp. 57-63 ◽  
Author(s):  
V. Sharma ◽  
M. Guleria ◽  
P. Chaudhary
Keyword(s):  
Large Scale ◽  
Quantitative Estimation ◽  
Aloe Vera ◽  
Shoot Proliferation ◽  
Multiple Shoots ◽  
Fold Increase ◽  
Nutrient Media ◽  
Aloe Barbadensis ◽  
Shoot Bud

Aloe vera(Liliaceae) is well known for its medicinal properties. It contains flavonoids, saponins, tannins, alkaloids and other compounds which exhibit medicinal, cosmetic and pharmacological properties. There is a lack of production of aloe leaf to meet the industry demand and therefore the means to facilitate large-scale aloe cultivation schemes need to be developed. Because sexual reproduction by seeds is almost ineffective inAloe veradue to male sterility and vegetative propagation by offshoots is only possible during the growing seasons, there is a need to develop a propagation method to facilitate large-scale cultivation.In vitroregeneration of medicinal plants is important and there is the potential for the production of high-quality plant-based medicine. In the present study, an attempt has been made to enhancein vitrocaulogenesis and aloin content in plants in the presence of a precursor (tryptophan) in the nutrient media. Forin vitroculture establishment and shoot bud multiplication, MS basal media were used supplemented with different concentrations and different combinations of growth regulators such as BAP (6-benzylaminopurine) and IAA (3-indole acetic acid). Shoot proliferation was optimal in MS medium containing 2.0 mg l−1BAP. For evaluation ofin vitroenhancement of aloin production the precursor tryptophan was added to the nutrient media at different levels (5, 10, 15 and 20 mg l−1). Addition of 20 mg l−1tryptophan induced a 2.43-fold increase in aloin content in multiple shoots cultures ofAloe barbadensis.

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