Antagonism of isolates of Bacillus subtilis to Phytophthora cactorum

1984 ◽  
Vol 62 (5) ◽  
pp. 1032-1035 ◽  
Author(s):  
R. S. Utkhede
Keyword(s):  
Bacillus Subtilis ◽  
Inhibition Zone ◽  
Crown Rot ◽  
Root Weight ◽  
Phytophthora Cactorum ◽  
Sclerotium Cepivorum ◽  
Greenhouse Trial ◽  
Bacterium Bacillus Subtilis ◽  
Bacterium Bacillus

Twenty-one isolates of the bacterium Bacillus subtilis isolated from local and exotic sclerotia of Sclerotium cepivorum were tested on cornmeal agar (CMA) for antagonism to six isolates of Phytophthora cactorum, causal agent of apple crown rot. All bacterial isolates produced diffusible antibiotics antagonistic to growth of all P. cactorum isolates on CMA. Antagonists were further evaluated in a replicated greenhouse trial for their ability to control crown rot infection on McIntosh apple seedlings in sterile soil. Six isolates of B. subtilis provided statistically significant reductions of infection on McIntosh seedlings. No correlation was observed between width of inhibition zone in vitro and protection from P. cactorum infections in McIntosh apple seedlings in a greenhouse trial. Significant negative correlations were observed between percent seedling infection and root weight.

scholarly journals UJI AKTIVITAS ANTIBAKTERI SPRAY BAU KAKI EKSTRAK KULIT JERUK NIPIS (Citrus aurantifolia) DENGAN VARIASI Gelling agent TERHADAP BAKTERI Bacillus subtilis

2021 ◽  
Vol 9 (1) ◽  
pp. 18-26
Author(s):  
Ade Maria Ulfa ◽  
◽  
Nofita Nofita ◽  
Bangun Saras Sandi ◽  
◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Bacterial Infections ◽  
Inhibition Zone ◽  
Gelling Agent ◽  
Apocrine Glands ◽  
Citrus Aurantifolia ◽  
Bacterium Bacillus Subtilis ◽  
Bacterium Bacillus ◽  
Lime Peel ◽  
Peel Extract

ABSTRACT Sweat is produced by the apocrine glands, if infected by bacteria that play a role in the decay process will certainly produce foot odor. Some of the bacteria that cause, including Staphylococcus epidermis, Corynebacterium acne and there is one bacterium that causes pungent foot odor that is Bacillus subtilis. Bacillus subtilis enzyme leucine dehydrogenase produced the highest, resulting in isovaleric acid foot odor. Lime peel (Citrus aurantifolia) has the potential to be developed for the antibacterial active ingredient of foot odor contained in tannins, alkaloids and flavonoids. Spray can be effective for inhibition of feet due to water fleas or bacterial infections. The purpose of this research is to test the inhibitory zone of the preparation of foot odor spray ethanol extract of lime peel (Citrus aurantifolia) with variations of gelling agent. Bacterial inhibition zone testing on extracts of lime peel spray preparations using the disc method. This test was carried out on spray with extract concentration of 0% extract base carbopol, 0% extract base HPMC, 0.2% extract base carbopol, 0.2% extract base HPMC, 0.4% extract base carbopol, 0.4% extract base HPMC and positive control with an average inhibition zone of 9,13 mm, 9,12 mm, 11,86 mm, 11,29 mm, 13,17 mm, 12,30 mm, 8,13 mm against the bacterium Bacillus subtilis. Antibacterial test results were analyzed using ONE WAY ANOVA, the results of statistical analysis on the preparation of lime peel extract showed a significant inhibition zone difference of 0.000 (P = <0.05) between all concentrations. Lime peel extract spray is effective in inhibiting the bacterium Bacillus subtilis. Key words: Sweat of foot odor, Lime skin (Citrus aurantifolia), Spray, bacteri Bacillus subtilis

scholarly journals Interaction of Bacillus subtilis CsaA with SecA and precursor proteins

2000 ◽  
Vol 348 (2) ◽  
pp. 367-373 ◽  
Author(s):  
Jörg P. MÜLLER ◽  
Jörg OZEGOWSKI ◽  
Stefan VETTERMANN ◽  
Jelto SWAVING ◽  
Karel H. M. VAN WELY ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Specific Interaction ◽  
Membrane Vesicles ◽  
Protein Export ◽  
E Coli ◽  
Bacterium Bacillus Subtilis ◽  
Bacterium Bacillus

CsaA from the Gram-positive bacterium Bacillus subtilis has been identified previously as a suppressor of the growth and protein-export defect of Escherichia coli secA(Ts) mutants. CsaA has chaperone-like activities in vivo and in vitro. To examine the role of CsaA in protein export in B. subtilis, expression of the csaA gene was repressed. While export of most proteins remained unaffected, export of at least two proteins was significantly reduced upon CsaA depletion. CsaA co-immunoprecipitates and co-purifies with the SecA proteins of E. coli and B. subtilis, and binds the B. subtilis preprotein prePhoB. Purified CsaA stimulates the translocation of prePhoB into E. coli membrane vesicles bearing the B. subtilis translocase, whereas it interferes with the SecB-mediated translocation of proOmpA into membrane vesicles of E. coli. The specific interaction with the SecA translocation ATPase and preproteins suggests that CsaA acts as a chaperone that promotes the export of a subset of preproteins in B. subtilis.

scholarly journals In vitro and in vivo antibiofilm potential of 2,4-Di- tert -butylphenol from seaweed surface associated bacterium Bacillus subtilis against group A streptococcus

2016 ◽  
Vol 191 ◽  
pp. 19-31 ◽  
Author(s):  
Dharmaprakash Viszwapriya ◽  
Udayakumar Prithika ◽  
Sundaresan Deebika ◽  
Krishnaswamy Balamurugan ◽  
Shunmugiah Karutha Pandian
Keyword(s):  
Bacillus Subtilis ◽  
Group A Streptococcus ◽  
Group A ◽  
Bacterium Bacillus Subtilis ◽  
Bacterium Bacillus

scholarly journals Functional investigation of Bacillus subtilis YrkF’s involvement in sulfur transfer reactions

Peptidomics ◽  
2016 ◽  
Vol 2 (1) ◽  
Author(s):  
Hannah L. Martin ◽  
Katherine A. Black ◽  
Patricia C. Dos Santos
Keyword(s):  
Bacillus Subtilis ◽  
Bioinformatic Analysis ◽  
Transfer Reactions ◽  
Initial Reaction ◽  
Cysteine Desulfurase ◽  
Sulfur Transfer ◽  
Bacterium Bacillus Subtilis ◽  
Bacterium Bacillus ◽  
Functional Investigation

AbstractSulfur incorporation into the molybdenum cofactor (Moco) in the Gram-negative bacterium Escherichia coli involves six enzymes. The initial reaction includes the cysteine desulfurase IscS, the sulfurtransferase TusA, and the rhodanese domaincontaining protein YnjE. The Gram-positive bacterium Bacillus subtilis contains no direct homologs for IscS, but rather four distinct cysteine desulfurases (YrvO, NifS, NifZ, SufS) and YrkF, a two-domain rhodanese protein with an N-terminal domain similar to TusA. Bioinformatic analysis was used to identify potential enzymes involved in the B. subtilis Moco thiolation pathway and in vitro reactions demonstrated that YrkF can accept sulfur from and enhance the activity of YrvO.

scholarly journals ANTIBACTERIAL ACTIVITY OF THE MEDICINAL PLANT, ACALYPHA FRUTICOSA FORSSK.

2016 ◽  
Vol 3 (2) ◽  
pp. 72-74
Author(s):  
Thambiraj J
Keyword(s):  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Medicinal Plant ◽  
Pathogenic Bacteria ◽  
Antibacterial Properties ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Root Extracts ◽  
Bacterium Bacillus Subtilis ◽  
Bacterium Bacillus

The present study is to confirm the antibacterial efficacy of root extracts of the folklore medicinal plant species, Acalypha fruticosa by using three alcoholic solvents viz; petroleum ether, ethyl acetate and methanol were tested against ten human pathogenic bacteria viz., Pseudomonas aeruginosa, P. stutzeri,Escherichia coli, Micrococcus sp., Lactobacillus sp., Servatia sp., Moraxetta sp., Bacillus subtilis, B. thuriengensis, and Klebsiella pneumoniae for assessing the antibacterial properties by adapting disc diffusion method. The results of the study revealed that all extracts showed varied degree of antibacterial activity against the tested pathogens. However, the methanol extract exhibited higher inhibition zone (21.83 mm) against the bacterium, Bacillus subtilis. This result supports the therapeutic importance of the species, Acalypha fruticosa in curing infectious diseases and encourages the extensive use of this species in health carepractices.

scholarly journals Release factor-dependent ribosome rescue by BrfA in the Gram-positive bacterium Bacillus subtilis

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Naomi Shimokawa-Chiba ◽  
Claudia Müller ◽  
Keigo Fujiwara ◽  
Bertrand Beckert ◽  
Koreaki Ito ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Stop Codon ◽  
Release Factor ◽  
Positive Bacterium ◽  
Gram Positive ◽  
E Coli ◽  
Cryo Electron Microscopy ◽  
Dead End ◽  
Bacterium Bacillus Subtilis ◽  
Bacterium Bacillus

AbstractRescue of the ribosomes from dead-end translation complexes, such as those on truncated (non-stop) mRNA, is essential for the cell. Whereas bacteria use trans-translation for ribosome rescue, some Gram-negative species possess alternative and release factor (RF)-dependent rescue factors, which enable an RF to catalyze stop-codon-independent polypeptide release. We now discover that the Gram-positive Bacillus subtilis has an evolutionarily distinct ribosome rescue factor named BrfA. Genetic analysis shows that B. subtilis requires the function of either trans-translation or BrfA for growth, even in the absence of proteotoxic stresses. Biochemical and cryo-electron microscopy (cryo-EM) characterization demonstrates that BrfA binds to non-stop stalled ribosomes, recruits homologous RF2, but not RF1, and induces its transition into an open active conformation. Although BrfA is distinct from E. coli ArfA, they use convergent strategies in terms of mode of action and expression regulation, indicating that many bacteria may have evolved as yet unidentified ribosome rescue systems.

Sign in / Sign up

Export Citation Format

Share Document