scholarly journals ANTIBACTERIAL ACTIVITY OF THE MEDICINAL PLANT, ACALYPHA FRUTICOSA FORSSK.

2016 ◽  
Vol 3 (2) ◽  
pp. 72-74
Author(s):  
Thambiraj J
Keyword(s):  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Medicinal Plant ◽  
Pathogenic Bacteria ◽  
Antibacterial Properties ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Root Extracts ◽  
Bacterium Bacillus Subtilis ◽  
Bacterium Bacillus

The present study is to confirm the antibacterial efficacy of root extracts of the folklore medicinal plant species, Acalypha fruticosa by using three alcoholic solvents viz; petroleum ether, ethyl acetate and methanol were tested against ten human pathogenic bacteria viz., Pseudomonas aeruginosa, P. stutzeri,Escherichia coli, Micrococcus sp., Lactobacillus sp., Servatia sp., Moraxetta sp., Bacillus subtilis, B. thuriengensis, and Klebsiella pneumoniae for assessing the antibacterial properties by adapting disc diffusion method. The results of the study revealed that all extracts showed varied degree of antibacterial activity against the tested pathogens. However, the methanol extract exhibited higher inhibition zone (21.83 mm) against the bacterium, Bacillus subtilis. This result supports the therapeutic importance of the species, Acalypha fruticosa in curing infectious diseases and encourages the extensive use of this species in health carepractices.

scholarly journals STUDY OF ANTIMICROBIAL ACTIVITY OF THE FOLKLORE MEDICINAL PLANT, ACALYPHA FRUTICOSA FORSSK.

2017 ◽  
Vol 4 (2) ◽  
pp. 57-60
Author(s):  
Thambiraj J
Keyword(s):  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Medicinal Plant ◽  
Ethyl Acetate ◽  
Pathogenic Bacteria ◽  
Antimicrobial Properties ◽  
Pathogenic Fungi ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Rhizopus Sp

The purpose of the study is to examine the antimicrobial efficacy of root extracts of the folklore medicinal plant species, Acalypha fruticosa Forssk by using three alcoholic solvents viz; petroleum ether, ethyl acetate and methanol were tested against ten human pathogenic bacteria viz., Pseudomonas aeruginosa, P. stutzeri, Escherichia coli, Micrococcus sp., Lactobacillus sp., Servatia sp., Moraxetta sp., Bacillus subtilis, B. thuriengensis, and Klebsiella pneumoniae and ten human pathogenic fungi viz., Aspergillus niger, A. flavus, A.baumannii, Fusarium oxysporum, F. solani, Mucor rouxii, Alternaria alternata, Candida albicans, Cladosporium sp. and Rhizopus sp. for assessing the antimicrobial properties by adapting disc diffusion method. The results of the study revealed that all extracts showed varied degree of antimicrobial activity against the tested pathogens. However, the methanol extracts exhibited higher inhibition zone (21.83 mm) against the bacterium, Bacillus subtilis and ethyl acetate extracts showed higher inhibiotion zone (24.83 mm) against the fungus, Rhizopus sp. Results concluded that this spices contain high amount of secondary metabolites due to these metabolites they have high antimicrobial activity and it can be used as good bio- preservater and it can also use for medicinal purpose.

scholarly journals COMPARATIVE STUDY OF ANTIBACTERIAL PROPERTIES OF FLAVONOIDS OF LEAVES FROM DIFFERENT CACTUS, PERENNIAL GRASSES AND MEDICINAL PLANT

2018 ◽  
Vol 6 (3) ◽  
pp. 32-41 ◽  
Author(s):  
Premlata Singariya ◽  
Mourya Krishan Kumar ◽  
Padma Kumar
Keyword(s):  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Medicinal Plant ◽  
Comparative Study ◽  
Proteus Mirabilis ◽  
Pathogenic Bacteria ◽  
Antibacterial Properties ◽  
Perennial Grasses ◽  
Sensitive Test ◽  
Pathogenic Microbes

Comparative study of antibacterial properties of flavonoids of leaves from different Cactus (Euphorbia caducifolia); perennial grasses(Panicum antidotale and Lasiurus sindicus) and medicinal plant (Anaegissus rotundifolia) against some important bacteria (G+ve or G-ve) was done. Flavonoid extracts of leaves from all the selected plants were found to possess strong antibacterial activity against these test pathogenic microbes, as revealed by ZOI=(mm± SD), AI, MIC=(mg/ml), MMC=(mg/ml) and TA=(ml/gm) of extracts against each sensitive test pathogenic bacteria were also evaluated. Most of the pathogens were found to be sensitive against these flavonoid extracts. Free flavonoid extract of grasses and medicinal plant showed higher activity against most of the pathogens. Euphorbia caducifolia is the most inactive plant in this study. All pathogens didn’t show any activity against Euphorbia caducifolia except Proteus mirabilis which show 7.83±0.21 mm ZOI for free flavonoid. Highest ZOI (18.67±0.26) was recorded for free flavonoid of medicinal plant (Anaegissus rotundifolia) against Bacillus subtilis (G+ve bacteria) as well as other parameters like AI (1.245), MIC (0.039 mg/ml), MMC (0.78 mg/ml) were found higher against Bacillus subtilisand highest TA (1012.821 ml/gm) were found highest for free flavonoid of Lasiurus sindicus against Bacillus subtilis. The findings of the present study suggested the exploitation of flavonoid extracts of Anaegissus rotundifoliaand of Lasiurus sindicusfor future antibacterial drugs.

scholarly journals ANTIMICROBIAL EFFICACY OF THE FOLKLORE MEDICINAL PLANT, ACACIA CAESIA (L.) WILD

2015 ◽  
Vol 2 (2) ◽  
pp. 110-113
Author(s):  
Thambiraj J ◽  
Paulsamy S
Keyword(s):  
Bacillus Subtilis ◽  
Ethyl Acetate ◽  
Pathogenic Bacteria ◽  
Antimicrobial Properties ◽  
Pathogenic Fungi ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Mucor Rouxii ◽  
Bacterium Bacillus ◽  
Rhizopus Sp

eaf extracts of the folklore plant species, Acacia caesia L. by using three alcoholic solvents viz; petroleum ether, ethyl acetate and methanol were tested against ten human pathogenic bacteria viz., Pseudomonas aeruginosa, P. stutzeri, Escherichia coli, Micrococcus sp., Lactobacillus sp., Servatia sp., Moraxetta sp., Bacillus subtilis, B. thuriengensis, and Klebsiella pneumoniae and ten human pathogenic fungi viz., Aspergillus niger, A. flavus, A.baumannii, Fusarium oxysporum, F. solani, Mucor rouxii, Alternaria alternata, Candida albicans, Cladosporium sp. and Rhizopus sp. for assessing the antimicrobial properties by adapting disc diffusion method. The results of the study revealed that all extracts showed varied degree of antimicrobial activity against the tested pathogens. However, the ethyl acetate extracts exhibited higher inhibition zone (15.73 mm) against the bacterium, Bacillus subtilis and the fungus, Mucor rouxii (20.67 mm). These results support the therapeutic importance of the species, Acacia caesia in curing infectious diseases and encourage the extensive use of this species in health care practices.

scholarly journals UJI AKTIVITAS EKSTRAK DAUN PIRDOT (Saurauia vulcani Korth) TERHADAP PERTUMBUHAN BAKTERI Bacillus subtilis

2020 ◽  
Vol 4 (1) ◽  
pp. 1
Author(s):  
Ulfayani Mayasari, M.Si
Keyword(s):  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Experimental Method ◽  
Extraction Method ◽  
Diffusion Method ◽  
Clear Zone ◽  
Agar Diffusion ◽  
Bacterium Bacillus Subtilis ◽  
Agar Diffusion Method ◽  
Bacterium Bacillus

<p><a name="_Hlk26926554"></a><span>Pirdot is a plant that has been widely used by people to treat various diseases. Pirdot leaves extract contain of flavonoids which have antibacterial Substances. This research has a purpose to determine the activity of pirdot leaves extract (Saurauia vulcani Korth.) inhibit to<span>  </span>bacterium Bacillus subtilis. This research uses an experimental method that are the maceration extraction method and testing the antibacterial activity by agar diffusion method. Result of the research was found that the inhibition area of pirdot leaves extract against Bacillus subtilis bacteria that have some clear zone<span>  </span>were 8.66 mm, 9.43 mm, 9.90 mm, 10.97 mm and 11.66 mm. Pirdot leaves can inhibit the growth of Bacillus subtilis bacteria.</span></p><p><span><span>Keywords: Pirdot leaf, Bacillus subtilis, antibacterial.</span></span></p>

scholarly journals UJI AKTIVITAS ANTIBAKTERI SPRAY BAU KAKI EKSTRAK KULIT JERUK NIPIS (Citrus aurantifolia) DENGAN VARIASI Gelling agent TERHADAP BAKTERI Bacillus subtilis

2021 ◽  
Vol 9 (1) ◽  
pp. 18-26
Author(s):  
Ade Maria Ulfa ◽  
◽  
Nofita Nofita ◽  
Bangun Saras Sandi ◽  
◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Bacterial Infections ◽  
Inhibition Zone ◽  
Gelling Agent ◽  
Apocrine Glands ◽  
Citrus Aurantifolia ◽  
Bacterium Bacillus Subtilis ◽  
Bacterium Bacillus ◽  
Lime Peel ◽  
Peel Extract

ABSTRACT Sweat is produced by the apocrine glands, if infected by bacteria that play a role in the decay process will certainly produce foot odor. Some of the bacteria that cause, including Staphylococcus epidermis, Corynebacterium acne and there is one bacterium that causes pungent foot odor that is Bacillus subtilis. Bacillus subtilis enzyme leucine dehydrogenase produced the highest, resulting in isovaleric acid foot odor. Lime peel (Citrus aurantifolia) has the potential to be developed for the antibacterial active ingredient of foot odor contained in tannins, alkaloids and flavonoids. Spray can be effective for inhibition of feet due to water fleas or bacterial infections. The purpose of this research is to test the inhibitory zone of the preparation of foot odor spray ethanol extract of lime peel (Citrus aurantifolia) with variations of gelling agent. Bacterial inhibition zone testing on extracts of lime peel spray preparations using the disc method. This test was carried out on spray with extract concentration of 0% extract base carbopol, 0% extract base HPMC, 0.2% extract base carbopol, 0.2% extract base HPMC, 0.4% extract base carbopol, 0.4% extract base HPMC and positive control with an average inhibition zone of 9,13 mm, 9,12 mm, 11,86 mm, 11,29 mm, 13,17 mm, 12,30 mm, 8,13 mm against the bacterium Bacillus subtilis. Antibacterial test results were analyzed using ONE WAY ANOVA, the results of statistical analysis on the preparation of lime peel extract showed a significant inhibition zone difference of 0.000 (P = <0.05) between all concentrations. Lime peel extract spray is effective in inhibiting the bacterium Bacillus subtilis. Key words: Sweat of foot odor, Lime skin (Citrus aurantifolia), Spray, bacteri Bacillus subtilis

scholarly journals Antibacterial Activity Test of Bacteriocin from Lactobacillus brevis, Lactobacillus casei and Lactobacillus plantarum Against Gram Positive Pathogenic Bacteria

2018 ◽  
Vol 3 (3) ◽  
pp. 85
Author(s):  
Novi Permata Sari ◽  
Rafika Sari ◽  
Eka Kartika Untari
Keyword(s):  
Antibacterial Activity ◽  
Lactobacillus Plantarum ◽  
Lactobacillus Casei ◽  
Pathogenic Bacteria ◽  
Heating Temperature ◽  
Lactobacillus Brevis ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Activity Test ◽  
Ph Range

Bacteriocin is a secondary metabolite product of lactic acid bacteria (LAB) which have an antimicrobial and potentially as a natural preservative. LAB isolates used in this study were Lactobacillus brevis, Lactobacillus casei and Lactobacillus plantarum. This study aimed to determine the antibacterial activity of bacteriocin produced by each isolate of LAB including the influence of pH and heating variation against Bacillus cereus, Bacillus subtilis and Staphylococcus epidermidis. Antibacterial activity test was done by using disc diffusion method. method. Confirmation test using proteolytic enzyme aimed to analyse that the inhibition zone produced from the activity of bacteriocin. The inhibition zone produced from L. brevis, L. casei and L. plantarum against B. cereus were 15.70, 16.43 and 14.50 mm, against B. subtilis were 13.37, 14.10 and 12.53 mm and against S. epidermidis were 11.37, 14.50 and 12.45 mm. The activity of each bacteriocin decreased with the addition of trypsin and catalase, bacteriocin was active in the pH range of 2-10 and heating temperature of 40-121oC. Statistical test showed that the addition of trypsin, catalase and the variation of pH also heating had significant differences (p<0.05) to antibacterial activity produced by bacteriocin from L. brevis, L. casei and L. plantarum. 

scholarly journals AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL BIJI KAPULAGA JAWA (Amomum compactum Soland. ex Maton) TERHADAP Streptococcus pyogenes

EKOLOGIA ◽  
2020 ◽  
Vol 20 (1) ◽  
pp. 31-39
Author(s):  
Oom Komala ◽  
. Ismanto ◽  
Muhammad Alan Maulana
Keyword(s):  
Antibacterial Activity ◽  
Streptococcus Pyogenes ◽  
Pathogenic Bacteria ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
Dilution Method ◽  
Natural Treatment ◽  
Amomum Compactum

Streptococcus pyogenes is one of the pathogenic bacteria that causes pharyngitis. Natural treatment to overcome these problems is to use cardamom seeds. The purpose of this study was to test the antibacterial activity, determine the concentration of inhibitory zone and phytochemical compounds from  ethanol 96% extract of Java cardamom seeds (Amomum compactum Soland. Ex Maton) against Streptococcus pyogenes. The method is used   solid dilution and paper disc diffusion method. The solid dilution method is used for the Minimum Inhibitory Concentration (MIC) test with a concentration of 1.25%, 2.5%, 5% and 7.5% while the paper diffusion method is used for the Inhibition zone Diameter (IZD)  using five treatments namely three concentrations of ethanol 96% extract of Java cardamom seeds (7.5%, 10% and 12%), one positive control of amoxicillin 0.01 mg/mL and one negative control of sterile distilled water. IZD data were  analyzed using ANOVA with a confidence level of 95% and α = 0.05 and Duncan's further tests to determine differences between treatments. The results showed that the MIC  was at a concentration of 7.5% while for the IZD test which had the highest activity there was a concentration of 12% with an average inhibition diameter of 12.03 ± 0.14 mm. In addition, ethanol 96% extract of Java cardamom seeds contain alkaloids, flavonoids, terpenoids and tannins which function in antibacterial activity.

Synthesis and Antibacterial Activities of N-Phenylpyrazolines from Veratraldehyde

2017 ◽  
Vol 901 ◽  
pp. 124-132
Author(s):  
Artania Adnin Tri Suma ◽  
Tutik Dwi Wahyuningsih ◽  
Deni Pranowo
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Bacillus Cereus ◽  
Shigella Flexneri ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Agar Well Diffusion Method

Some novel N-phenylpyrazolines were synthesized and investigated for their antibacterial activitiy. Chalcones 2-4 which were prepared from acetophenone and veratraldehyde derivatives were reacted with phenylhydrazine to give N-phenylpyrazolines 5-7. All of the synthesized compounds were characterized using FTIR, GC-MS, and NMR spectrometers. Further, antibacterial activity of N-phenylpyrazolines were evaluated by agar well-diffusion method against Staphylococcus aureus, Bacillus cereus, Bacillus subtilis, Escherichia coli, and Shigella flexneri. The highest activity (highest inhibition zone) of compound 5 was 2.6 mm (at 1000 ppm) against B. subtillis, compound 6 was 7.25 mm (at 1000 ppm) against S. aureus, and compound 7 was 6.75 mm (at 500 ppm) against S. aureus. The results indicated that compound 6 and 7 exhibited promising antibacterial activity.

scholarly journals Combined activity of Azadirachta indica and Dodonea viscose leaves extract on some selected pathogens

2020 ◽  
Vol 12 (1) ◽  
pp. 31-38
Author(s):  
A. I Isah ◽  
A.I. Aminu
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Petroleum Ether ◽  
Azadirachta Indica ◽  
Pathogenic Bacteria ◽  
Bacterial Species ◽  
Plant Secondary Metabolites ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Plant Materials

The study was undertaken to investigate the antimicrobial activity both individually and in combination of two Nigerian plants (Azadirachta indica and Dodonea viscosa) against a variety of pathogenic bacteria. Five bacterial species namely; Staphylococcus aureus, Escherichia coli, Salmonella typhi, Klebsiella pneumoniae and Pseudomonas aeruginosa were selected for the assays. The powdered plant materials of the two plants were extracted using sohxlet extraction technique with methanol water and petroleum ether as solvents. The crude extracts of the two plants were subjected to phytochemical screening for qualitative detection of plant secondary metabolites. The extracts were further tested for antibacterial activity against the selected pathogens singly and then combined using agar well diffusion method. Antibacterial activity of the two plants singly indicates that the methanolic extract possess the highest antibacterial activity at a concentration of 50mg/ml with an inhibition zone of 23.3±0.5mm compared to aqueous extract with 21.7±0.5mm inhibition zone at a concentration of 50mg/ml. The least activity was observed with the petroleum ether extract with an inhibition zone of 7.3±0.5mm at a concentration of 12.5mg/ml. Combination of the plants extracts exhibited lower antibacterial activity on the test isolates compared to single plants as evidenced by the production of lower inhibition zones. Key words: Azadirachta indica, Dodonea viscosa, pathogens, antimicrobial activity, synergistic effect

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