Growth and osmoregulation of Boekelovia hooglandii in relation to salinity

1994 ◽  
Vol 72 (6) ◽  
pp. 823-828 ◽  
Author(s):  
Shuhei Fujii ◽  
Johan A. Hellebust
Keyword(s):  
Amino Acids ◽  
Sodium Chloride ◽  
Chloride Concentration ◽  
Salinity Range ◽  
Sodium Chloride Concentration ◽  
Spectroscopy Analysis ◽  
Cell Extracts ◽  
Gas Chromatography Mass Spectroscopy ◽  
Mass Spectroscopy Analysis ◽  
Chloride Concentrations

The growth and osmoregulation of Boekelovia hooglandii Nicolai et Baas Becking (Chrysophyceae) was investigated as a function of salinity. This chromophyte microflagellate was found to be euryhaline and also to require sodium for growth. The optimum sodium chloride concentration for growth was 0.2–0.4 M, and growth was severely inhibited in media with sodium chloride concentrations above 1 M. Using gas chromatography – mass spectroscopy analysis of cell extracts, it was established that the alga contains D-mannitol, myo-inositol, as well as the inositol derivative, cyclohexanetetrol. The content of cyclohexanetetrol and mannitol increased with increased salinity, while the content of inositol remained almost constant. Potassium and magnesium were the major intracellular cations. However, the content of cations and amino acids showed only minor increases with salinity over a wide salinity range. The contents of cyclohexanetetrol and mannitol increased rapidly when cells were subjected to hypertonic shocks. It was concluded that B. hooglandii utilizes cyclohexanetetrol and mannitol as osmoregulatory substances. Key words: Boekelovia hooglandii, chromophyte, cyclohexanetetrol, euryhaline, D-mannitol, myo-inositol, osmoregulation, salinity.

Effect of Sodium Chloride on Limulus Amebocyte Lysate

1979 ◽  
Vol 41 (02) ◽  
pp. 329-336 ◽  
Author(s):  
Raymond R Schleef ◽  
Dianne M Kenney ◽  
David Shepro
Keyword(s):  
Sodium Chloride ◽  
Chloride Concentration ◽  
Clot Formation ◽  
Sodium Chloride Concentration ◽  
Limulus Amebocyte Lysate ◽  
Chloride Concentrations

SummaryConcentrations of sodium chloride up to 3 M increase the time necessary for the clot formation from Limulus amebocyte lysate (LAL) induced with endotoxin. Sodium chloride at a concentration of 4 M prevents clot formation by either precipitation or denaturation of procoagulase. The time necessary for the activation of procoagulase by endotoxin is increased by a change in the sodium chloride concentration from 0.15 M to 0.588 M. No effect on the proteolytic phase or the polymerization phase of the clotting reaction is detected by the increase in sodium chloride concentration from 0.15 M to 0.588 M. The authors conclude that increased sodium chloride concentrations may aid the isolation of procoagulase.

Effect of sodium chloride on growth and methane production of methanogens

1977 ◽  
Vol 23 (7) ◽  
pp. 893-897 ◽  
Author(s):  
G. B. Patel ◽  
L. A. Roth
Keyword(s):  
Sodium Chloride ◽  
Methane Production ◽  
Chloride Concentration ◽  
Methanobacterium Thermoautotrophicum ◽  
Good Growth ◽  
Optimum Growth ◽  
Sodium Chloride Concentration ◽  
Inhibition Of Growth ◽  
Pure Cultures ◽  
Chloride Concentrations

The effect of up to 263.7 mM sodium chloride on the growth and methane production by pure cultures of Methanospirillum hungatii GP1, Methanobacterium MOH, Methanobacterium thermoautotrophicum, and an unidentified methanogen was studied. Growth and methane production by M. hungatii GP1 were not affected up to 97.3 mM NaCl but there was some inhibition of growth at higher concentrations. Growth of Methanobacterium MOH was independent of sodium chloride concentration within the range investigated. For the unidentified methanogen, optimum growth and methane production occurred at 15.2 mM NaCl, while growth of M. thermoautotrophicum was not affected by sodium chloride concentrations up to 15.2 mM. Concentrations over 15.2 mM were inhibitory to these two organisms. The 15 mM sodium chloride concentration used by some investigators appears suitable for isolation and cultivation of methanogens since all the organisms tested in this study exhibited good growth and methane production at this salt concentration.

scholarly journals Plant Response to Saline Substrates V. Chloride Regulation in the Individual Organs of Hordeum Vulgare During Treatment with Sodium Chloride

1965 ◽  
Vol 18 (3) ◽  
pp. 505 ◽  
Author(s):  
H Greenway ◽  
DA Thomas
Keyword(s):  
Sodium Chloride ◽  
Hordeum Vulgare ◽  
Chloride Concentration ◽  
Plant Response ◽  
Sodium Chloride Concentration ◽  
High Sodium ◽  
The Individual ◽  
Chloride Regulation ◽  
Chloride Concentrations

This is a study on the regulation of chloride concentrations in H. vulgare at the early tillering stage, when grown on media of high sodium chloride concentration. 36CI was used during certain periods to determine retranslocation.

scholarly journals Characterization of Virus Adsorption by Using DEAE-Sepharose and Octyl-Sepharose

2002 ◽  
Vol 68 (8) ◽  
pp. 3965-3968 ◽  
Author(s):  
Patricia A. Shields ◽  
Samuel R. Farrah
Keyword(s):  
Sodium Chloride ◽  
Electrostatic Interactions ◽  
Hydrophobic Interactions ◽  
Chloride Concentration ◽  
Sodium Chloride Concentration ◽  
Virus Adsorption ◽  
Nacl Concentration

ABSTRACT Viruses were characterized by their adsorption to DEAE-Sepharose or by their elution from octyl-Sepharose by using buffered solutions of sodium chloride with different ionic strengths. Viruses whose adsorption to DEAE-Sepharose was reduced most rapidly by an increase in the sodium chloride concentration were considered to have the weakest electrostatic interactions with the solids; these viruses included MS2, E1, and φX174. Viruses whose adsorption to DEAE-Sepharose was reduced least rapidly were considered to have the strongest electrostatic interactions with the column; these viruses included P1, T4, T2, and E5. All of the viruses studied adsorbed to octyl-Sepharose in the presence of 4 M NaCl. Viruses that were eluted most rapidly following a decrease in the concentration of NaCl were considered to have the weakest hydrophobic interactions with the column; these viruses included φX174, CB4, and E1. Viruses that were eluted least rapidly from the columns after the NaCl concentration was decreased were considered to have the strongest hydrophobic interactions with the column; these viruses included f2, MS2, and E5.

scholarly journals GC/MS Analysis of n-Hexane and Chloroform Extracts of Chenopodium murale Leaves in Iraq

2019 ◽  
pp. 1-6
Author(s):  
Muthanna Saadi Farhan ◽  
Amjed Haseeb Khamees ◽  
Omar Hussein Ahmed ◽  
Amani AmerTawfeeq ◽  
Yahya Saad Yaseen
Keyword(s):  
Chemical Constituents ◽  
Chloroform Extract ◽  
Chemical Components ◽  
Bioactive Components ◽  
Spectroscopy Analysis ◽  
Nitrogenous Compounds ◽  
Alpha Pinene ◽  
Chenopodium Murale ◽  
Gas Chromatography Mass Spectroscopy ◽  
Mass Spectroscopy Analysis

Chenopodium murale L. it is an essential annual herbaceous weed belongs to the genus Chenopodium and family Chenopodiaceae. Chenopodium murale L. commonly called as nettle leaf goosefoot. Aim of this study is the gas chromatography-mass spectroscopy analysis of chemical constituents of Chenopodium murale leaves in two different extracts; n-hexane and chloroform. These extracts contain 37 chemical components which are Monoterpenes, steroids precursor and fatty acids. Furthermore the n- hexane extract revealed about 35.22% of cyclic and acyclic monoterpenoids, fatty acid about 2.07%, also 2.31% of nitrogenous compounds and sterol precursor about 0.41%. However the chloroform extract revealed the presence of linolenic acid representing 13.54% and neo menthol representing 18.87%, also the other minor components are carvone oxide (0.27%), alpha- pinene epoxide (1.71%), Trans- Squalene (0.77%) and other minor bioactive components.

scholarly journals Plant Response to Saline Substrates II. Chloride, Sodium, and Potassium Uptake and Translocation in Young Plants of Hordeum Vulgare During and After a Short Sodium Chloride Treatment

1962 ◽  
Vol 15 (1) ◽  
pp. 39 ◽  
Author(s):  
H Greenway
Keyword(s):  
Sodium Chloride ◽  
Hordeum Vulgare ◽  
Salinity Stress ◽  
Chloride Concentration ◽  
Plant Response ◽  
Potassium Uptake ◽  
Sodium Chloride Concentration ◽  
Subsequent Response ◽  
Chloride Treatment ◽  
Sodium And Potassium

Young barley plants, Hordeum vulgare cv. Chevron, were subjected to a sodium chloride concentration of 100 m-equiv/l. In a "continued" treatment, the salinity stress was maintained for 15 days. In a "removed" treatment, sodium chloride was removed from the substrate after 5 days, and the subsequent response was studied over a period of 10 days.

Effect of sodium chloride concentration and temperature on melphalan stability during storage and use

1994 ◽  
Vol 51 (21) ◽  
pp. 2701-2704
Author(s):  
F. Pinguet ◽  
P. Martel ◽  
P. Rouanet ◽  
M. Fabbro ◽  
C. Astre
Keyword(s):  
Sodium Chloride ◽  
Chloride Concentration ◽  
Sodium Chloride Concentration
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