scholarly journals Characterization of Virus Adsorption by Using DEAE-Sepharose and Octyl-Sepharose

2002 ◽  
Vol 68 (8) ◽  
pp. 3965-3968 ◽  
Author(s):  
Patricia A. Shields ◽  
Samuel R. Farrah
Keyword(s):  
Sodium Chloride ◽  
Electrostatic Interactions ◽  
Hydrophobic Interactions ◽  
Chloride Concentration ◽  
Sodium Chloride Concentration ◽  
Virus Adsorption ◽  
Nacl Concentration

ABSTRACT Viruses were characterized by their adsorption to DEAE-Sepharose or by their elution from octyl-Sepharose by using buffered solutions of sodium chloride with different ionic strengths. Viruses whose adsorption to DEAE-Sepharose was reduced most rapidly by an increase in the sodium chloride concentration were considered to have the weakest electrostatic interactions with the solids; these viruses included MS2, E1, and φX174. Viruses whose adsorption to DEAE-Sepharose was reduced least rapidly were considered to have the strongest electrostatic interactions with the column; these viruses included P1, T4, T2, and E5. All of the viruses studied adsorbed to octyl-Sepharose in the presence of 4 M NaCl. Viruses that were eluted most rapidly following a decrease in the concentration of NaCl were considered to have the weakest hydrophobic interactions with the column; these viruses included φX174, CB4, and E1. Viruses that were eluted least rapidly from the columns after the NaCl concentration was decreased were considered to have the strongest hydrophobic interactions with the column; these viruses included f2, MS2, and E5.

scholarly journals Plant Response to Saline Substrates II. Chloride, Sodium, and Potassium Uptake and Translocation in Young Plants of Hordeum Vulgare During and After a Short Sodium Chloride Treatment

1962 ◽  
Vol 15 (1) ◽  
pp. 39 ◽  
Author(s):  
H Greenway
Keyword(s):  
Sodium Chloride ◽  
Hordeum Vulgare ◽  
Salinity Stress ◽  
Chloride Concentration ◽  
Plant Response ◽  
Potassium Uptake ◽  
Sodium Chloride Concentration ◽  
Subsequent Response ◽  
Chloride Treatment ◽  
Sodium And Potassium

Young barley plants, Hordeum vulgare cv. Chevron, were subjected to a sodium chloride concentration of 100 m-equiv/l. In a "continued" treatment, the salinity stress was maintained for 15 days. In a "removed" treatment, sodium chloride was removed from the substrate after 5 days, and the subsequent response was studied over a period of 10 days.

Effect of sodium chloride concentration and temperature on melphalan stability during storage and use

1994 ◽  
Vol 51 (21) ◽  
pp. 2701-2704
Author(s):  
F. Pinguet ◽  
P. Martel ◽  
P. Rouanet ◽  
M. Fabbro ◽  
C. Astre
Keyword(s):  
Sodium Chloride ◽  
Chloride Concentration ◽  
Sodium Chloride Concentration

Studies on the Growth of Stichococcus Bacillaris Naeg in Culture

1974 ◽  
Vol 54 (2) ◽  
pp. 261-268 ◽  
Author(s):  
J. Hayward
Keyword(s):  
Sodium Chloride ◽  
High Salinity ◽  
Chloride Concentration ◽  
Sodium Chloride Concentration ◽  
Freshwater Organism ◽  
Ionic Concentrations ◽  
Morphogenetic Effects ◽  
Stichococcus Bacillaris

Stichococcus bacillaris has been grown under conditions of varying ionic concentrations. Sodium chloride appears to be the dominant compound in determining growth of this euryhaline alga and from the reaction to increased sodium chloride concentration it is postulated that S. bacillaris is a freshwater organism which can tolerate high salinities. Some morphogenetic effects of high salinity are described.

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