scholarly journals Response of microbial communities to roxarsone under different culture conditions

2017 ◽  
Vol 63 (8) ◽  
pp. 661-670 ◽  
Author(s):  
Yaci Liu ◽  
Zhaoji Zhang ◽  
Yasong Li ◽  
Yuhong Fei
Keyword(s):  
Microbial Community ◽  
High Throughput Sequencing ◽  
Poultry Manure ◽  
Inorganic Arsenic ◽  
Culture Conditions ◽  
Feed Additive ◽  
Aerobic Incubation ◽  
Anaerobic Conditions ◽  
Sequencing Technology ◽  
Acinetobacter Spp

Roxarsone is a feed additive widely used in the broiler and swine industries that has the potential to contaminate the environment, mainly via the use of poultry manure as fertilizer, which results in release of inorganic arsenic to the soil and water. This study was conducted to investigate roxarsone degradation and the response of the microbial community under different culture conditions using high-throughput sequencing technology. Poultry litter was incubated for 288 h in the presence of roxarsone under light aerobic, dark aerobic, or dark anaerobic conditions. The results showed that roxarsone was completely degraded after 48 h of dark anaerobic incubation, while 79.9% and 94.5% of roxarsone was degraded after 288 h of dark aerobic and light aerobic incubation, respectively. Under dark aerobic conditions with microbial inhibitor sodium azide, roxarsone was rarely degraded during the 288 h of incubation, illustrating that microorganisms play an important role in roxarsone degradation. Microbial community structure was significantly different among various culture conditions. Olivibacter, Sphingobacterium, and Proteiniphilum were the top 3 genera in the control samples. Sphingobacterium and Alishewanella dominated the light aerobic samples, while the dominant microflora of the dark aerobic samples were Acinetobacter spp. Pseudomonas and Advenella were the predominant genera of dark anaerobic samples. This study emphasizes the potential importance of microbes in roxarsone degradation and expands our current understanding of microbial ecology during roxarsone degradation under different environmental conditions.

Bacterial Community Analysis of Mine Contaminated Soils in Hechi City

2020 ◽  
Vol 14 (4) ◽  
pp. 476-486
Author(s):  
Tingting Liu ◽  
Caoping Pang ◽  
Fengcai Ye ◽  
Dafei Gong ◽  
Jieling Luo ◽  
...  
Keyword(s):  
Microbial Community ◽  
Genetic Engineering ◽  
Bacterial Community ◽  
Contaminated Soils ◽  
High Throughput Sequencing ◽  
Community Analysis ◽  
Microbial Community Analysis ◽  
Mine Soils ◽  
Sequencing Technology ◽  
Autonomous Region

Four mine contaminated soils located in northwest of Guangxi autonomous region were selected for microbial community analysis. These mine soils were contaminated by chromium (Cr) and cadmium (Cd). Microbial communities were described by high-throughput sequencing technology, which showed 39 different phyla in four samples. Among these phyla, Proteobacteria was the most abundant phylum in all samples. Acidobacteria, Actinobacteria, Planctomycetes, Firmicutes, Gemmatimonadetes, Bacteroidetes and Chloroflexi showed higher relative abundances than other phyla. In addition, a wide diversity of bacteria with the potential of bioremediation, such as Sphingomonas, Lysobacter and Gemmatimonas were detected in the tested mine contaminated soils. The results of microbial community analysis will provide a new target for isolation of microorganisms with the potential of bioremediation and lay the foundation for a great enhancement of bioremediation ability through the genetic engineering modification of indigenous microorganisms in future.

scholarly journals Research on the Influence of Different Temperature Conditions on the Microbial Community in Anearobic-Anoxic-Oxic process on the Plateau

2020 ◽  
Author(s):  
Decai Huang ◽  
Yongchen Zong ◽  
Ning Zhang ◽  
Yuanwei Li ◽  
Kaiyue Hao
Keyword(s):  
Microbial Community ◽  
High Throughput Sequencing ◽  
Treatment Process ◽  
Working Condition ◽  
Ammonia Oxidation ◽  
Waste Water Treatment ◽  
Sequencing Technology ◽  
Temperature Conditions ◽  
Water Treatment Process ◽  
Nitrite Oxidizing Bacteria

AbstractIn order to further understand the influence of different temperature conditions in the low temperature range on the microbial community structure in the Anearobic-Anoxic-Oxic waste water treatment process on the plateau, four temperature conditions were designed in the research, including 25°C, 20°C, 15°C and 10°C. Each working condition lasted three days. Activated sludge from anaerobic tank, anoxic tank and oxic tank under each working condition was analyzed based on the 16S rRNA high-throughput sequencing technology. The result shows that the plateau temperature conditions have little influence on the level abundance of phylum. Under all conditions, Bacteroidetes, Proteobacteria and Actinobacteria are the main phyla. The abundance of nitrifying bacterium and phosphorus removal bacteria rose in the first three conditions and fell at 10 °C. The abundance of Denitrification bacteria and Nitrite oxidizing bacteria was significantly higher than that of Ammonia oxidation bacteria and Azotobacter bacteria and Phosphorus-accumulating Organisms(PAO) had an overall advantage over Glycogen-accumulating Organisms (GAO) throughout the research.

scholarly journals Analysis of Microbial Diversity and Variation Law During Xiaoqu Baijiu Fermentation using High-throughput Sequencing Technology

2021 ◽  
Author(s):  
Qing Wang ◽  
Xiaoqing Xiang ◽  
PengFei Wu ◽  
Guoqiang Han
Keyword(s):  
Microbial Community ◽  
Microbial Diversity ◽  
High Throughput ◽  
Relative Abundance ◽  
High Throughput Sequencing ◽  
Sequencing Technology ◽  
Theoretical Support ◽  
Brewing Process ◽  
Dominant Bacteria ◽  
Brewing Time

Abstract In this study, high-throughput sequencing (HTS) was used to compare and analyze the microbial diversity and variation law during the brewing process of xiaoqu Baijiu. The results showed that 34 phyla, 378 genera of bacteria and 4 phyla, 32 genera of fungi were detected. At the phylum level, Firmicutes, Proteobacteria, Bacteroidetes, Ascomycota and Bacteroidetes were the dominant groups. During the brewing process of xiaoqu Baijiu, the dominant bacteria were Weissella and unidentified Rickettsiales 2 days before brewing and Lactobacillus 3 days after brewing until the end of brewing. The dominant fungi were Rhizopus, Saccharomyces and Issatchenkia. The relative abundance of Rhizopus decreased with the extension of brewing time, while the relative abundance of Saccharomyces increased and became the dominant bacteria after the second day of brewing. This study revealed the diversity and variation of microbial community in the brewing process of xiaoqu Baijiu, and provide theoretical support and lay the foundation for future study on the contribution of microbial metabolism during brewing of xiaoqu Baijiu, thereby promote the development of xiaoqu baijiu industry.

scholarly journals Comparison of Different Enhanced Coagulation Methods for Azo Dye Removal from Wastewater

2019 ◽  
Vol 11 (17) ◽  
pp. 4760 ◽  
Author(s):  
Xinhao Luo ◽  
Chen Liang ◽  
Yongyou Hu
Keyword(s):  
Microbial Community ◽  
Congo Red ◽  
High Throughput Sequencing ◽  
Treatment Plant ◽  
Iron Sulfate ◽  
Sequencing Technology ◽  
Enhanced Coagulation ◽  
Practical Applications ◽  
Printing And Dyeing Wastewater ◽  
Raw Wastewater

Printing and dyeing wastewater (PDW) is considered to be one of the most difficult industrial wastewaters to treat because of its large quantities, high pH values, and high color and toxicity, which may endanger the lives of animals and humans. In this study, we assessed the chemical decolorization process of Congo Red in azo dyes using response surface methodology (RSM), and the effect of different enhanced coagulation pretreatment processes (ECPPs) on the microbial community structure of PDW using high-throughput sequencing technology. We concluded that, based on the initial concentration and pH of Congo Red, different decolorants can be selected for decolorization reactions. In addition, it was found that the microbial community of the wastewater after three different ECPP treatments was similar to the raw wastewater and the oxidation ditch wastewater from a treatment plant. We also found that the ECPPs with polymeric iron sulfate had the smallest effect on the microbial community. In practical applications, these findings provide a reference for an established link between the physicochemical and biochemical treatment of PDW.

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