Seasonal variations in tissue metabolic capacities of yellow perch (Perca flavescens) from clean and metal-contaminated environments

2003 ◽  
Vol 60 (3) ◽  
pp. 269-278 ◽  
Author(s):  
Debbie Audet ◽  
Patrice Couture
Keyword(s):  
Seasonal Variations ◽  
Yellow Perch ◽  
Citrate Synthase ◽  
Feeding Activity ◽  
Perca Flavescens ◽  
Nucleoside Diphosphate Kinase ◽  
Fish Muscle ◽  
Sodium Potassium ◽  
Metal Concentrations ◽  
Liver Copper

This study examined seasonal variations in tissue metal contamination and physiological condition of yellow perch (Perca flavescens) from a clean lake (Halfway Lake) and a metal-contaminated environment (Whitson Lake) in spring, summer, and fall of 1999. Dietary metal content influenced liver metal concentrations, especially for cadmium. Fulton's condition factor (FCF), an indicator of recent feeding activity, was lower in Whitson fish except in summer, when higher FCF values corresponded with higher liver copper concentrations, presumably because of higher feeding rate. Tissue protein concentrations and indicators of biosynthetic capacities (nucleoside diphosphate kinase and RNA/DNA ratios) suggested lower biosynthesis in Whitson fish. Muscle aerobic and anaerobic capacities (using citrate synthase and lactate dehydrogenase as indicators, respectively) were consistently lower in Whitson fish, with maximal differences in summer. In contrast, although liver aerobic capacities were generally comparable among populations, anaerobic capacities were much higher in Whitson fish. Finally, gill sodium/potassium adenosinetriphosphatase (Na+/K+ ATPase) activity peaked in the spring in fish from both lakes, and higher activities were correlated with elevated gill copper concentrations. This study highlights the importance of seasonal variations in tissue metal concentrations and fish condition. This information is essential to evaluate the extent of impairment in condition faced by metal-contaminated wild fish.

Effects of environmental metal contamination on the condition, swimming performance, and tissue metabolic capacities of wild yellow perch (Perca flavescens)

2002 ◽  
Vol 59 (8) ◽  
pp. 1296-1304 ◽  
Author(s):  
James W Rajotte ◽  
Patrice Couture
Keyword(s):  
Yellow Perch ◽  
Citrate Synthase ◽  
Swimming Performance ◽  
Perca Flavescens ◽  
Nucleoside Diphosphate Kinase ◽  
Metal Exposure ◽  
Mitochondrial Enzymes ◽  
Fast Start ◽  
Condition Indices ◽  
Adenosine Triphosphate Production

In this study, the general condition, swimming capacities, and tissue metabolic capacities and metal concentrations in wild yellow perch (Perca flavescens) were examined from a range of metal-contaminated lakes near Sudbury, Ontario. Fish exposed to elevated environmental cadmium and copper concentrations showed higher liver concentrations of these metals and lower condition indices. Because growth rate appeared lower in the most metal-contaminated fish, the high tissue activities of nucleoside diphosphate kinase, an indicator of biosynthesis, in these fish indicated an increased rate of protein turnover and suggested a bioenergetic cost of metal exposure. Yellow perch from the most metal-contaminated lakes exhibited lower aerobic capacities, as indicated by citrate synthase and β-hydroxyacyl coenzyme A dehydrogenase activities, two mitochondrial enzymes involved in aerobic adenosine triphosphate production, and by critical swimming speed. There was no evidence from our data that environmental metal exposure affected anaerobic capacities of tissues as measured by lactate dehydrogenase activities or anaerobic fast-start swimming performance. Overall, these data show that metal exposure leads to measurable effects on metabolic capacities in wild yellow perch.

Effect of acute and chronic temperature transition on enzymes of cardiac metabolism in white perch (Morone americana), yellow perch (Perca flavescens), and smallmouth bass (Micropterus dolomieui)

1991 ◽  
Vol 69 (1) ◽  
pp. 258-262 ◽  
Author(s):  
Dawn H. Sephton ◽  
William R. Driedzic
Keyword(s):  
Yellow Perch ◽  
Citrate Synthase ◽  
White Perch ◽  
Perca Flavescens ◽  
Smallmouth Bass ◽  
Transferase Activity ◽  
Morone Americana ◽  
Coa Transferase ◽  
Micropterus Dolomieui ◽  
The Impact

White perch (Morone americana), yellow perch (Perca flavescens), and smallmouth bass (Micropterus dolomieui) were acclimated to 5 and 20 °C. There was an increase in ventricle mass relative to body mass in smallmouth bass only following acclimation to 5° C. Maximal in vitro activities of hexokinase, citrate synthase, carnitine acyl CoA transferase (with palmitoyl CoA, palmitoleoyl CoA, and oleoyl CoA as substrates), and total ATPase were assessed in crude heart homogenates. Tissues removed from warm-acclimated animals were tested at 20 and 5 °C; tissues removed from cold-acclimated animals were assessed at 5 °C. Acute temperature transitions were associated with decreases in the activities of hexokinase (Q10 ≈ 1.8), citrate synthase (Q10 ≈ 1.4), and ATPase (Q10 ≈ 1.7). The impact of temperature on carnitine acyl CoA transferases was generally less severe. This suggests that maximal fatty acid oxidation is conserved better than glucose oxidation during a warm to cold transition. Maximal enzyme activities were generally unaffected by the acclimation regime, with the exception of that of carnitine acyl CoA transferase in white perch heart. The substantial increase in carnitine acyl CoA transferase activity when unsaturated CoA derivatives were provided as substrate suggests an increased capacity to oxidize unsaturated fatty acids at low temperature following an acclimation period. Attempts to sustantiate this contention by offering labelled oleic acid to ventricle sheets were thwarted by a high rate of incorporation into the total lipid pool.

Transcriptional responses to environmental metal exposure in wild yellow perch (Perca flavescens) collected in lakes with differing environmental metal concentrations (Cd, Cu, Ni)

Ecotoxicology ◽  
2009 ◽  
Vol 18 (5) ◽  
pp. 620-631 ◽  
Author(s):  
Fabien Pierron ◽  
Vincent Bourret ◽  
Jérôme St-Cyr ◽  
Peter G. C. Campbell ◽  
Louis Bernatchez ◽  
...  
Keyword(s):  
Yellow Perch ◽  
Perca Flavescens ◽  
Metal Exposure ◽  
Transcriptional Responses ◽  
Metal Concentrations

Metal bioaccumulation and oxidative stress in yellow perch (Perca flavescens) collected from eight lakes along a metal contamination gradient (Cd, Cu, Zn, Ni)

2005 ◽  
Vol 62 (3) ◽  
pp. 563-577 ◽  
Author(s):  
Anik Giguère ◽  
Peter GC Campbell ◽  
Landis Hare ◽  
Carole Cossu-Leguille
Keyword(s):  
Oxidative Stress ◽  
Metal Ion ◽  
Yellow Perch ◽  
Perca Flavescens ◽  
Metal Exposure ◽  
Metal Concentrations ◽  
Individual Level ◽  
Ion Concentrations ◽  
Free Metal ◽  
Free Metal Ion

Links among metal exposure, metal accumulation, and metal-induced effects were explored in indigenous yellow perch (Perca flavescens) collected from eight lakes located along a metal concentration gradient in two mining regions. Fish exposure to Cd, Cu, Ni, and Zn was estimated on the basis of calculated free metal ion concentrations in lake waters. Hepatic metal concentrations were determined and various markers of oxidative stress were measured to assess metal effects in liver cells. Accumulated metals were not consistently related to ambient free metal ion concentrations, possibly because of unaccounted for fluctuations in lake water metal concentrations. Accumulated metals were associated with limited oxidative stress in perch livers, as evidenced by reductions in glutathione concentrations and glutathione reductase activities. However, this stress appeared to be minor, since increasing hepatic Cu concentrations were associated with reduced lipid peroxidation, a response opposite to that predicted from basic principles. Our results suggest that oxidative stress will not have direct repercussions on the health of the perch at the individual level. We speculate that the observed increase in metallothionein concentrations with increasing accumulated metals might afford protection against reactive oxygen species.

Daily Ration of Yellow Perch (Perca flavescens) from Lake Memphremagog, Quebec–Vermont, with a Comparison of Methods for In Situ Determinations

1978 ◽  
Vol 35 (12) ◽  
pp. 1597-1603 ◽  
Author(s):  
Brian S. Nakashima ◽  
William C. Leggett
Keyword(s):  
Body Weight ◽  
Body Size ◽  
Yellow Perch ◽  
Diet Composition ◽  
Seasonal Pattern ◽  
Feeding Activity ◽  
Perca Flavescens ◽  
Complex Method ◽  
Daily Ration

In situ estimates of daily ration for yellow perch (Perca flavescens) range from a high of 5.5–6.7% body weight in July to a low of 2.2–2.4% body weight in October. The seasonal pattern corresponds well to known patterns of growth. Comparison of three methods for in situ determination of daily ration levels indicated the method outlined here and the more complex method of Thorpe yield similar results. The method of Keast and Welsh and derivatives of this method which correct for digestion between sampling periods give unreliable values that are 50% below the other two and, in general, are below maintenance ration levels. Diet composition and feeding activity varied seasonally and with body size. Key words: body size relationships, diet composition, seasonality

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