Species Differences of Some Members of Salmonidae Based on Their Muscle Myogen Patterns

1963 ◽  
Vol 20 (1) ◽  
pp. 101-104 ◽  
Author(s):  
H. Tsuyuki ◽  
E. Roberts
Keyword(s):  
Atlantic Salmon ◽  
Gel Electrophoresis ◽  
Brook Trout ◽  
Species Differences ◽  
Pink Salmon ◽  
Starch Gel Electrophoresis ◽  
Protein Patterns ◽  
Dolly Varden ◽  
Constant Property ◽  
Starch Gel

The muscle myogen patterns obtained by starch gel electrophoresis of the Dolly Varden and eastern brook trout (Salvelinus), the rainbow and steelhead trouts and the Atlantic salmon (Salmo), and the coho, spring, chum, sockeye and pink salmon (Oncorhynchus) are compared. The myogen protein patterns are clearly distinct for each species of fishes studied, except for the rainbow and steelhead trouts, and constitute a remarkably constant property which can be employed to differentiate between species and for other useful purposes. The specific and generic inter-relationships have been analyzed by these patterns and developed as further criteria for classification purposes and are discussed in conjunction with currently accepted beliefs.

Multiple Haemoglobins of the Atlantic Salmon (Salmo salar)

1968 ◽  
Vol 25 (12) ◽  
pp. 2651-2663 ◽  
Author(s):  
N. P. Wilkins
Keyword(s):  
Life Cycle ◽  
Atlantic Salmon ◽  
Salmo Salar ◽  
Gel Electrophoresis ◽  
Initial Increase ◽  
Small Fish ◽  
Starch Gel Electrophoresis ◽  
Complete Development ◽  
Starch Gel ◽  
The Individual

The haemoglobins of over 500 salmon of different lengths, from Scotland, Greenland, and Canada have been analysed by vertical starch–gel electrophoresis at pH 8.1. Complex ontogenetic variations, involving an initial increase and later reduction in the number of fractions evident, have been observed among the anodally migrating haemoglobins. The variations observed have been correlated with changes in length, and the complete development of the anodal haemoglobin complex from the single fraction of small fish to the nine-fraction pattern of adults is outlined. The individual haemoglobin fractions appear to represent structurally distinct molecules whose regulated occurrence at different phases of the life cycle is discussed at the individual and population levels.

A Simple Microelectrophoretic Method Using Starch-Agar Gel

1964 ◽  
Vol 10 (1) ◽  
pp. 62-68 ◽  
Author(s):  
Tatsuo Hase
Keyword(s):  
Quantitative Analysis ◽  
Ultraviolet Light ◽  
Gel Electrophoresis ◽  
Protein Analysis ◽  
Starch Gel Electrophoresis ◽  
Protein Patterns ◽  
Agar Gel ◽  
Starch Gel ◽  
Agar Gel Electrophoresis ◽  
Starch Agar

Abstract A simple microelectrophoretic method of protein analysis using the gel of starch agar mixture is described. The method is easily standardized and the results show consistency and reproducibility. Protein patterns obtained show higher resolution than those of paper or agar-gel electrophoresis and simulate that of starch-gel electrophoresis. Developed protein patterns are suitable to quantitative analysis by appropriate electrophotometric or ultraviolet-light scanning devices and can be preserved as permanent records.

Some aspects of the biology of the fungal symbiont of Sirex noctilio

1966 ◽  
Vol 14 (1) ◽  
pp. 25 ◽  
Author(s):  
JM King
Keyword(s):  
Gel Electrophoresis ◽  
Starch Gel Electrophoresis ◽  
Fungal Symbiont ◽  
Protein Patterns ◽  
Sirex Noctilio ◽  
Agar Culture ◽  
Parenchyma Cells ◽  
Ray Parenchyma ◽  
Starch Gel ◽  
Ray Parenchyma Cells

Studies of the cultural characteristics on artificial media of the fungal symbiont of Sirex noctilio F. indicate that in Australia only one fungus is involved in the association. A medium containing 20 p.p.m. o-phenylphenol was found suitable for the isolation of the Sirex fungus. Histological studies confirmed that the Sirex fungus develops in wood only as vegetative hyphae. Ray parenchyma cells were killed in advance of the growing hyphae, which suggested that a toxin was produced by the fungus. Comparisons of growth in agar culture, of fructifications produced on wood blocks, and of starch gel electrophoresis protein patterns indicated that the Sirex fungus is probably a strain or variety of Arnylostereum chailletii (Fr.) Boidin.

scholarly journals 238 Isoperoxidase and Protein Patterns in Compatible and Incompatible Pear/Quince Graft Combinations

HortScience ◽  
1999 ◽  
Vol 34 (3) ◽  
pp. 483B-483
Author(s):  
Hatice Gulen ◽  
Chon C. Lim ◽  
Rajeev Arora ◽  
Hatice Gulen ◽  
Ali Kuden ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Starch Gel Electrophoresis ◽  
Protein Profiles ◽  
Protein Patterns ◽  
Graft Union ◽  
Peroxidase Isozymes ◽  
Sds Page ◽  
Starch Gel ◽  
Graft Compatibility ◽  
General Protein

The similarity or differences of peroxidase isozymes in rootstocks and scions may influence their graft compatibility. This study was conducted to identify peroxidase isozymes and/or other proteins that may be used as markers to predict compatibility between pear and various quince clones. `Bartlett' (BT) and `Beurre Hardy' (BH) pear cultivars were budded on 13 selected quince clones and quince A (QA) rootstocks; BT and BH cultivars are known to be incompatible and compatible, respectively, with quince root stocks. Bark and cambial tissues were taken from unbudded rootstocks, scions, and 4 cm above and below the graft union for isozyme analysis. Samples were collected 1, 2, 3, and 12 months after grafting. In addition, samples from the graft unions were also analyzed 12 months after grafting. Isozyme separation was performed by starch gel electrophoresis. Many isozyme bands were commonly observed in the two scions; however, one anodal peroxidase was detected in BH but not in BT samples. This isozyme was also detected in QA and in all but four quince clones. Protein profiles of bark tissues from QA and three pear scions (BT, `Bosc', and P. crassane) were determined using SDS-PAGE. In general, protein profiles of the three pear cultivars appeared remarkably similar; however, P. crassane (a compatible pear cultivar on QA) had a 63 kDa protein, which was absent in BT and faintly observed in `Bosc' (intermediate compatibility). Our results suggest that these isoperoxidase and polypeptide could be associated with pear/quince graft compatibility.

Fractionation of Plasminogen by Starch Gel Electrophoresis

1964 ◽  
Vol 12 (01) ◽  
pp. 126-136 ◽  
Author(s):  
Karl H. Slotta ◽  
J. D Gonzalez
Keyword(s):  
Gel Electrophoresis ◽  
Room Temperature ◽  
Broad Band ◽  
Caproic Acid ◽  
Starch Gel Electrophoresis ◽  
Full Activity ◽  
Veronal Buffer ◽  
Starch Gel ◽  
Alkaline Range

SummaryWhen urea or ε-amino caproic acid were used as solublizing agents for plasminogen in electrophoretic experiments, only one broad band of the proenzyme was obtained on acetate cellulose, in starch block, and in acrylamide gel. In starch gel electrophoresis, however, both forms of plasminogen – the native or euglobulin and Kline’s or Pseudoglobulin plasminogen – separated into six bands. These migrated toward the cathode at room temperature in borate or veronal buffer in the alkaline range and showed full activity in fibrinagar-streptokinase plates.

scholarly journals THE INHERITANCE OF ENZYME VARIANTS FOR TYROSINE AMINOTRANSFERASE, NADP-DEPENDENT MALATE DEHYDROGENASE, NADP-DEPENDENT ISOCITRATE DEHYDROGENASE, AND TETRAZOLIUM OXIDASE IN TETRAHYMENA PYRIFORMIS, SYNGEN 1

Genetics ◽  
1973 ◽  
Vol 74 (4) ◽  
pp. 595-603
Author(s):  
D Borden ◽  
E T Miller ◽  
D L Nanney ◽  
G S Whitt
Keyword(s):  
Detailed Analysis ◽  
Malate Dehydrogenase ◽  
Isocitrate Dehydrogenase ◽  
Gel Electrophoresis ◽  
Tetrahymena Pyriformis ◽  
Tyrosine Aminotransferase ◽  
Breeding Program ◽  
Starch Gel Electrophoresis ◽  
Enzyme Locus ◽  
Starch Gel

ABSTRACT The isozymic patterns of tyrosine aminotransferase, NADP malate dehydrogenase, NADP isocitrate dehydrogenase, and tetrazolium oxidase were examined by starch-gel electrophoresis in Tetrahymena pyriformis, syngen 1. The genetics of the alleles controlling these enzymes was studied through a breeding program. Each enzyme locus was shown to assort vegetatively, as do other loci in this organism. A detailed analysis of the assortment process for the tyrosine aminotransferase locus indicated that the rate of stabilization of heterozygotes into pure types was essentially identical to previously-reported rates for other loci.

STARCH GEL ELECTROPHORESIS OF MYOGEN FROM CHICKEN BREAST MUSCLE IN CONSTANT AND GRADIENT BUFFER SYSTEMS

1963 ◽  
Vol 41 (1) ◽  
pp. 369-387 ◽  
Author(s):  
J. M. Neelin
Keyword(s):  
Gel Electrophoresis ◽  
Protein Concentration ◽  
Breast Muscle ◽  
Chicken Breast ◽  
Starch Gel Electrophoresis ◽  
Two Dimensional ◽  
Sodium Cacodylate ◽  
Gradient Systems ◽  
Optimal Separation ◽  
Starch Gel

By varying conditions of starch gel electrophoresis, factors contributing to the resolution of myogen proteins from chicken breast muscle have been studied. Variables examined included composition of the myogen extractant, protein concentration, ionic strength of electrophoretic media, pH of gel media, plane and direction of electrophoresis, and the nature of cations and anions in gel media and bridge solutions. The significance of anions was more closely studied with constant buffer systems, and gradient systems in which bridge electrolyte differed from, and gradually altered, the gel medium. Optimal separation was obtained in gradient systems with 0.10 M sodium chloride bridge solutions, and gel media of sodium cacodylate, pH 6.9, μ 0.010, which resolved 12 cationic zones, and sodium veronal, pH 7.4, μ 0.010, which resolved 10 anionic zones. These buffers in two-dimensional sequence revealed a total of about 24 components in this myogen.

Morphological and biochemical systematics of chubs, Nocomis biguttatus and N. micropogon (Pisces: Cyprinidae), in southern Ontario

1981 ◽  
Vol 59 (5) ◽  
pp. 771-775 ◽  
Author(s):  
Moira M. Ferguson ◽  
David L. G. Noakes ◽  
Roy G. Danzmann
Keyword(s):  
Gel Electrophoresis ◽  
Function Analysis ◽  
Morphological Differentiation ◽  
Sexually Dimorphic ◽  
Starch Gel Electrophoresis ◽  
Southern Ontario ◽  
Electrophoretic Mobilities ◽  
Starch Gel ◽  
Respective Species ◽  
Probability Of Misclassification

Examination of 17 presumptive gene loci by starch-gel electrophoresis revealed differential mobilities only at acid phosphatase-1, alcohol dehydrogenase, esterase-1, and phosphoglucomutase between Nocomis biguttatus and N. micropogon. No intraspecific variation was observed for any loci. The genetic identity (I) and genetic distance (D) were 0.874 and 0.134, respectively. The correlation of electrophoretic mobilities and nuptial tubercle pattern in sexually dimorphic males supports the present taxonomic distinction of these species and provides a simple, unambiguous means of identifying any individuals.Stepwise discriminant function analysis of a series of mensural characters was used to compare fish identified as to species by electrophoresis. At best this correctly assigned fish to their respective species in 85.7% of cases, with a probability of misclassification of 0.1335.This study suggests these two are sibling species, based on a comparison of biochemical and morphological differentiation.

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