Need for Replicated Electrophoretic Analyses in Multiagency Genetic Stock Identification (GSI) Programs: Examples from a Pink Salmon (Oncorhynchus gorbuscha) GSI Fisheries Study

1991 ◽  
Vol 48 (8) ◽  
pp. 1396-1407 ◽  
Author(s):  
Bruce A. White ◽  
James B. Shaklee
Keyword(s):  
Pink Salmon ◽  
The Other ◽  
Oncorhynchus Gorbuscha ◽  
Stock Identification ◽  
Genetic Stock ◽  
Pink Salmon Oncorhynchus Gorbuscha ◽  
Genetic Stock Identification ◽  
Electrophoretic Data ◽  
Discrepancy Rate ◽  
Reported Data

Genetic stock identification (GSI) studies usually assume that electrophoretic data from different laboratories are accurate, precise, and comparable. We compared the genetic scores from replicate (identical) collections of pink salmon (Oncorhynchus gorbuscha) tissues that were analyzed in a "blind" manner by three different laboratories; scores at 15 genetic systems (18 loci) were compared for 1248 fish. The scores from laboratories A and B consistently agreed. Their average discrepancy rate across all loci was 0.21%, which was approximately 18 times lower than the discrepancy rate of laboratory C (3.86%). Laboratory C also reported data with higher numbers of discrepancies (P < 0.01) in their scores of variant genotypes (non-*100/100) than the other two laboratories. Stock composition estimates using maximum likelihood analysis of the data from laboratories A and B differed by approximately 1% by stock group, while those of laboratory C differed by an average of 8% by stock group from the estimates of the other two laboratories. Our findings demonstrate that different laboratories can, but do not always, produce results that are consistent. Because many GSI programs in North America rely on data contributed by several laboratories, we recommend that testing of replicate samples be incorporated into all multiagency GSI studies.

Biochemical Genetic Stock Identification of Pink Salmon (Oncorhynchus gorbuscha) in Southern British Columbia and Puget Sound

1985 ◽  
Vol 42 (9) ◽  
pp. 1474-1483 ◽  
Author(s):  
Terry D. Beacham ◽  
Ruth E. Withler ◽  
Allan P. Gould
Keyword(s):  
British Columbia ◽  
Pink Salmon ◽  
Puget Sound ◽  
Oncorhynchus Gorbuscha ◽  
Fraser River ◽  
Genetic Stock ◽  
Pink Salmon Oncorhynchus Gorbuscha ◽  
Allelic Frequencies ◽  
Genetic Stock Identification ◽  
Duplicated Loci

We used electrophoresis to examine genetic variability at 12 loci for 4 even-year broodline stocks and at 14 loci for 21 odd-year broodline stocks of pink salmon (Oncorhynchus gorbuscha) in southern British Columbia and Puget Sound. Allelic frequencies were most heterogeneous between the two pink salmon broodlines, but within the odd-year broodline, Fraser River, Canadian non-Fraser, and Puget Sound pink salmon had significantly different allelic frequencies at some loci. Canadian non-Fraser River stocks had the greatest heterogeneity of allelic frequencies within a region, whereas the Fraser River stocks had the least amount of heterogeneity. There was no significant two-locus linkage disequilibrium for the pink salmon stocks surveyed. Cluster analyses by allelic frequencies indicated that Fraser River, Canadian non-Fraser, and Puget Sound stocks were reasonably distinctive. We used differences in genotypic frequencies at 11 loci (3 were duplicated loci) to estimate stock composition of these three groups of pink salmon in mixtures in which the true compositions were known, and we were able to compare the accuracy and precision of our estimates with respect to mixture size and stock composition. We estimated that 100% of pink salmon sampled in a test fishery in Thompson Sound were of Canadian non-Fraser origin.

Outbreeding effect on embryo development in hybrids of allopatric pink salmon (Oncorhynchus gorbuscha) populations, a potential consequence of stock translocation

Aquaculture ◽  
2007 ◽  
Vol 272 ◽  
pp. S152-S160 ◽  
Author(s):  
Ivan A. Wang ◽  
Sara E. Gilk ◽  
William W. Smoker ◽  
Anthony J. Gharrett
Keyword(s):  
Embryo Development ◽  
Pink Salmon ◽  
Oncorhynchus Gorbuscha ◽  
Potential Consequence ◽  
Pink Salmon Oncorhynchus Gorbuscha

Juvenile Pacific Sandfish, Trichodon trichodon, Associated with Pink Salmon, Oncorhynchus gorbuscha, Fry in the Nearshore Area, Southeastern Alaska

Copeia ◽  
1983 ◽  
Vol 1983 (2) ◽  
pp. 549 ◽  
Author(s):  
Jack E. Bailey ◽  
Bruce L. Wing ◽  
Joyce H. Landingham
Keyword(s):  
Pink Salmon ◽  
Oncorhynchus Gorbuscha ◽  
Pink Salmon Oncorhynchus Gorbuscha ◽  
Nearshore Area

Comprehensive microsatellite baseline for genetic stock identification of Atlantic salmon (Salmo salar L.) in northernmost Europe

2017 ◽  
Vol 74 (8) ◽  
pp. 2159-2169 ◽  
Author(s):  
Mikhail Ozerov ◽  
Juha-Pekka Vähä ◽  
Vidar Wennevik ◽  
Eero Niemelä ◽  
Martin-A. Svenning ◽  
...  
Keyword(s):  
Atlantic Salmon ◽  
Salmo Salar ◽  
Stock Identification ◽  
Genetic Stock ◽  
Atlantic Salmon Salmo Salar ◽  
Genetic Stock Identification

Supplementary Checks on the Scales of Pink Salmon (Oncorhynchus gorbuscha) and Chum Salmon (O. keta)

1965 ◽  
Vol 22 (6) ◽  
pp. 1477-1489 ◽  
Author(s):  
H. T. Bilton ◽  
W. E. Ricker
Keyword(s):  
British Columbia ◽  
Chum Salmon ◽  
Pink Salmon ◽  
Oncorhynchus Gorbuscha ◽  
Commercial Fisheries ◽  
Pink Salmon Oncorhynchus Gorbuscha ◽  
Clear Cut ◽  
Second Year Of Life ◽  
Second Year ◽  
Similar Incidence

Among 159 central British Columbia pink salmon that had been marked by removal of two fins as fry and had been recovered in commercial fisheries after one winter in the sea, the scales of about one-third showed a supplementary or "false" check near the centre of the scale, in addition to the single clear-cut annulus. This evidence from fish of known age confirms the prevailing opinion that such extra checks do not represent annuli, hence that the fish bearing them are in their second year of life rather than their third. Unmarked pink salmon from the same area, and some from southern British Columbia, had a generally similar incidence of supplementary checks. In both marked and unmarked fish the supplementary checks varied in distinctness from faint to quite clear. In a sample of scales of 14 double-fin marked chum salmon which were known to be in their 4th year, all fish had the expected 3 annuli, and 12 fish had a supplementary check inside the first annulus.

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