Colocation between a gene encoding the bZip factor SPA and an eQTL for a high-molecular-weight glutenin subunit in wheat (Triticum aestivum)

Genome ◽  
2004 ◽  
Vol 47 (4) ◽  
pp. 705-713 ◽  
Author(s):  
Sabine Guillaumie ◽  
Gilles Charmet ◽  
Laurent Linossier ◽  
Valérie Torney ◽  
Nathalie Robert ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Triticum Aestivum ◽  
Single Nucleotide Polymorphism ◽  
Quantitative Trait ◽  
Storage Protein ◽  
Nucleotide Polymorphism ◽  
Gene Encoding ◽  
Single Nucleotide ◽  
Protein Activator ◽  
Bzip Factor

The quality of wheat grain is largely determined by the quantity and composition of storage proteins (prolamins) and depends on mechanisms underlying the regulation of expression of prolamin genes. The endosperm-specific wheat basic region leucine zipper (bZIP) factor storage protein activator (SPA) is a positive regulator that binds to the promoter of a prolamin gene. The aim of this study was to map SPA (the gene encoding bZIP factor SPA) and genomic regions associated with quantitative variations of storage protein fractions using F7 recombinant inbred lines (RILs) derived from a cross between Triticum aestivum 'Renan' and T. aestivum 'Récital'. SPA was mapped through RFLP using a cDNA probe and a specific single nucleotide polymorphism (SNP) marker. Storage protein fractions in the parents and RILs were quantified using capillary electrophoresis. Quantitative trait loci (QTLs) for protein were detected and mapped on six chromosome regions. One QTL, located on the long arm of chromosome 1B, explained 70% of the variation in quantity of the x subunit of Glu-B1. Genetic mapping suggested that SPA is located on chromosome arm 1L and is also present in the confidence interval of the corresponding QTL for Glu-B1x on 1BL, suggesting that SPA might be a candidate gene for this QTL.Key words: Triticum aestivum, quantitative trait locus (QTL), single nucleotide polymorphism (SNP), storage protein activator (SPA), high-molecular-weight glutenin subunit (HMW-GS).

scholarly journals Genetic polymorphism for IFNγ +874T/A in patients with acute toxoplasmosis

2012 ◽  
Vol 45 (6) ◽  
pp. 757-760 ◽  
Author(s):  
Elizabeth de Souza Neves ◽  
André Luis Land Curi ◽  
Maira Cavalcanti de Albuquerque ◽  
Cassius Schnel Palhano-Silva ◽  
Laura Berriel da Silva ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphism ◽  
Case Control Study ◽  
Severe Disease ◽  
Clinical Manifestations ◽  
Nucleotide Polymorphism ◽  
Gene Encoding ◽  
Single Nucleotide ◽  
Duration Of Disease ◽  
Acute Toxoplasmosis ◽  
Control Study

INTRODUCTION: A single nucleotide polymorphism (SNP) in the gene encoding gamma interferon influences its production and is associated with severity of infectious diseases. This study aimed to evaluate the association of IFNγ+874T/A SNP with duration of disease, morbidity, and development of retinochoroiditis in acute toxoplasmosis. METHODS: A case-control study was conducted among 30 patients and 90 controls. RESULTS: Although statistical associations were not confirmed, A-allele was more common among retinochoroiditis cases and prolonged illness, while T-allele was more frequent in severe disease. CONCLUSIONS: Despite few cases, the results could indicate a relation between IFNγ+874T/A single nucleotide polymorphism and clinical manifestations of toxoplasmosis.

The TG5 thyroglobulin gene test for a marbling quantitative trait loci evaluated in feedlot cattle

2004 ◽  
Vol 44 (7) ◽  
pp. 669 ◽  
Author(s):  
W. Barendse ◽  
R. Bunch ◽  
M. Thomas ◽  
S. Armitage ◽  
S. Baud ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphism ◽  
Quantitative Trait Loci ◽  
Dna Sequence ◽  
Dna Markers ◽  
Quantitative Trait ◽  
Allelic Association ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide ◽  
Trait Loci ◽  
Thyroglobulin Gene

The TG5 (thyroglobulin 5′ leader sequence) single nucleotide polymorphism has been associated with marbling in cattle fed for periods longer than 250 days. To test whether the association could be detected in diverse cattle, fed for less than 250 days, and to measure the size of the effect, we sampled 1750 cattle from the AMH Toowoomba feedlot. These cattle were sampled on 28 separate days, over 9 months. Their marbling scores covered the complete range. We found that the TG5 single nucleotide polymorphism was associated with marbling scores (P<0.05) and estimated that TG5 genotypes explained 6.5% of the residual deviance for the marbling phenotype. We also found that the '3' allele was more frequent in animals with higher marbling scores. The consistency of the allelic association between studies and, in particular, the association found in diverse cattle, indicate that the TG5 polymorphism can be used as a breeding tool and possibly a feedlot entry tool. To estimate the size of the genetic region in which the marbling quantitative trait loci are located, we tested the nearby DNA markers CSSM66 and BMS1747. These do not show allelic associations to marbling. The consistency of the allelic association between studies, the lack of association to nearby DNA markers and the complementary information on gene action of genes near Thyroglobulin suggest that DNA sequence variations, in or near the Thyroglobulin gene sequence, are the likely causes for the marbling quantitative trait loci. Further studies of single nucleotide polymorphism in and near the Thyroglobulin DNA sequence should allow causal mutations for the effect to be identified.

scholarly journals A Missense Single-Nucleotide Polymorphism in a Gene Encoding a Protein Tyrosine Phosphatase (PTPN22) Is Associated with Rheumatoid Arthritis

2004 ◽  
Vol 75 (2) ◽  
pp. 330-337 ◽  
Author(s):  
Ann B. Begovich ◽  
Victoria E.H. Carlton ◽  
Lee A. Honigberg ◽  
Steven J. Schrodi ◽  
Anand P. Chokkalingam ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Single Nucleotide Polymorphism ◽  
Protein Tyrosine Phosphatase ◽  
Tyrosine Phosphatase ◽  
Nucleotide Polymorphism ◽  
Gene Encoding ◽  
Single Nucleotide ◽  
Protein Tyrosine

scholarly journals Génomique des canards

2013 ◽  
Vol 26 (5) ◽  
pp. 391-402
Author(s):  
A. VIGNAL ◽  
C. DIOT ◽  
C. MOLETTE ◽  
M. MORISSON ◽  
T. FARAUT ◽  
...  
Keyword(s):  
Quantitative Trait Locus ◽  
Single Nucleotide Polymorphism ◽  
Quantitative Trait ◽  
Premier Lieu ◽  
Haut Débit ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide ◽  
Trait Locus

La démocratisation des outils de la génomique et plus particulièrement du séquençage à haut débit a permis le séquençage du génome du canard commun. Des projets complémentaires sont déjà initiés pour prolonger et exploiter au mieux ces premiers acquis. En tout premier lieu, il s’agit de poursuivre la description de la structure du génome et d’en exploiter les connaissances : cartes d’hybrides irradiés pour ordonner la séquence le long des chromosomes ; génomique comparée avec le génome de la poule pour bénéficier des connaissances sur ce génome modèle ; recherche de SNP (Single Nucleotide Polymorphism) pour les études et la gestion de populations ; carte génétique pour la détection des QTL (Quantitative Trait Locus). La première détection de QTL influençant les performances du mulard, réalisée à l’aide de marqueurs microsatellites chez la cane commune, sera complétée par une seconde étude utilisant des marqueurs SNP développés spécifiquement et permettant une bien meilleure couverture du génome. Par ailleurs, il est important de réaliser une annotation fonctionnelle du génome, ce qui peut être abordé par le séquençage de transcrits. A terme, le génome annoté sera utilisé pour analyser son expression dans différents tissus et/ou conditions d’élevage, la connaissance des modèles de transcrits et de protéines facilitant les études en transcriptomique et protéomique. Le canard mulard,  produit du croisement de la cane commune avec le canard de Barbarie, devra également être étudié en raison de son intérêt agronomique, lié à ses performances exceptionnelles dans la filière des palmipèdes gras.

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