Evaluation of genetic variability of wild hops (Humulus lupulus L.) in Canada and the Caucasus region by chemical and molecular methods

Genome ◽  
2010 ◽  
Vol 53 (7) ◽  
pp. 545-557 ◽  
Author(s):  
Josef Patzak ◽  
Vladimír Nesvadba ◽  
Karel Krofta ◽  
Alena Henychova ◽  
Arkady Inalovic Marzoev ◽  
...  
Keyword(s):  
North American ◽  
Molecular Genetic ◽  
Molecular Genetic Analysis ◽  
Humulus Lupulus ◽  
Chemical Characteristics ◽  
The Caucasus ◽  
Caucasus Region ◽  
Humulus Lupulus L ◽  
Molecular Genetic Data ◽  
Simple Sequence

Wild hops ( Humulus lupulus L.) are potential new germplasms to expand the variability of genetic resources for hop breeding. We evaluated Canadian (62 plants) and Caucasian (58 plants) wild hops by their chemical characteristics and with molecular genetic analyses using sequence-tagged site and simple sequence repeat markers, in comparison with European (104 plants) and North American (27 plants) wild hops. The contents of alpha and beta acids varied from 0.36% to 5.11% and from 0.43% to 6.66% in Canadian wild hops, and from 0.85% to 3.65% and from 1.22% to 4.81% in Caucasian wild hops, respectively. The contents of cohumulone and colupulone distinctly differed between European and North American wild hops: the cohumulone level in alpha acids was in the range 46.1%–68.4% among North American wild hops and in the range 13.6%–30.6% among European wild hops. The high content of myrcene and the low contents of humulene, farnesene, and selinenes were typical for wild hops from Canada, in contrast to wild hops from the Caucasus region. We compared the chemical characteristics with molecular genetic data. Chemical characteristics differentiated wild hops into North American and Eurasian groups. Molecular genetic analysis was able to separate Caucasian wild hops from European wild hops. We proved a hop phylogeny by means of wide molecular analysis.

A numerical analysis of morpho-geographic groups of cultivars of Humulus lupulus based on samples of cones

1981 ◽  
Vol 59 (3) ◽  
pp. 311-324 ◽  
Author(s):  
Ernest Small
Keyword(s):  
Numerical Analysis ◽  
North America ◽  
North American ◽  
New World ◽  
Geographical Origin ◽  
Humulus Lupulus ◽  
Numerical Analyses ◽  
Hybrid Origin ◽  
Morphological Examination ◽  
Humulus Lupulus L

Numerical analyses of hop (strobilus) samples showed that the geographical origin of cultivars of Humulus lupulus L. from North America, Britain, continental Europe, and Japan can be identified with considerable reliability on the basis of morphological examination. Samples of hybrid origin between North American and European plants tended to be similar to American cultivars, but often showed combinations of Old and New World characteristics, making their identification problematical.

scholarly journals A study of the genetic diversity in the world soybean collection using microsatellite markers associated with fungal disease resistance

2020 ◽  
Vol 181 (3) ◽  
pp. 81-90
Author(s):  
A. K. Zatybekov ◽  
Y. T. Turuspekov ◽  
B. N. Doszhanova ◽  
S. I. Abugalieva
Keyword(s):  
Disease Resistance ◽  
Ssr Markers ◽  
Molecular Genetic ◽  
Fungal Disease ◽  
Molecular Genetic Analysis ◽  
Fungal Diseases ◽  
Fungal Disease Resistance ◽  
Glycine Max L ◽  
Purple Seed Stain ◽  
Simple Sequence

Background. Soybean (Glycine max (L.) Merr.) gradually becomes one of the leading legume crops in Kazakhstan. The area under soybeans in the country has been increasing annually and requires the development of adapted cultivars with a higher yield, improved quality characters, and resistance to emerging fungal diseases. The enlargement of the crop’s gene pool also suggests the need to study and document local soybean accessions to meet the standards of the available world soybean collection by using reliable and informative types of DNA markers.Materials and methods. In this study, the soybean collection consisting of 288 accessions from different countries, including 36 cultivars and promising lines from Kazakhstan, was studied. The molecular genetic analysis was performed using nine polymorphic SSR (simple sequence repeats) markers, seven of which (Satt244, Satt565, Satt038, Satt309, Satt371, Satt570 and Sat_308) were associated with resistance to three main fungal diseases of soybean – frogeye leaf spot, fusarium root rot, and purple seed stain.Results. The average PIC (polymorphism information content) value of the analyzed SSR markers constituted 0.66 ± 0.07, confirming their highlevel polymorphism. The principal coordinate analysis suggested that the local accessions were genetically most close to the accessions from East Asia. As the collection showed a robust resistance to three studied fungal diseases in Almaty Region during 2018–2019, the distribution of the studied SSR markers in the population was not significantly associated with resistance to the analyzed diseases under field conditions.Conclusion. SSR genotyping of the soybean collection helped to identify accessions that potentially possess resistance-associated alleles of fungal disease resistance genes. The data obtained can be further used for the development of DNA documentation and the breeding the promising cultivars and lines of soybean. 

Genic SSRs for European and North American hop (Humulus lupulus L.)

2008 ◽  
Vol 55 (7) ◽  
pp. 959-969 ◽  
Author(s):  
Nahla V. Bassil ◽  
B. Gilmore ◽  
J. M. Oliphant ◽  
K. E. Hummer ◽  
J. A. Henning
Keyword(s):  
North American ◽  
Humulus Lupulus ◽  
Genic Ssrs ◽  
Humulus Lupulus L

MOLECULAR GENETIC HOP (HUMULUS LUPULUS L.) RESEARCH IN SLOVENIA

2005 ◽  
pp. 31-34 ◽  
Author(s):  
B. Javornik ◽  
J. Jakše ◽  
N. Štajner ◽  
P. Kozjak ◽  
A. Čerenak
Keyword(s):  
Molecular Genetic ◽  
Humulus Lupulus ◽  
Humulus Lupulus L

scholarly journals Evaluation of genetic variability within actual hop (Humulus lupulus L.) cultivars by an enlarged set of molecular markers

2018 ◽  
Vol 54 (No. 2) ◽  
pp. 86-91 ◽  
Author(s):  
J. Patzak ◽  
A. Henychová
Keyword(s):  
Genetic Variability ◽  
Expressed Sequence Tag ◽  
Molecular Genetic ◽  
Humulus Lupulus ◽  
Appropriate Technology ◽  
Brewing Industry ◽  
Breeding Lines ◽  
Humulus Lupulus L ◽  
Long Time ◽  
Expressed Sequence

Traditional hop (Humulus lupulus L.) cultivars have been used in the brewing industry for a long time. Globally, about ten new breeding lines were released to the market in each decade from ~1970 to 1999. Since 2006, the rate of release of new cultivars has increased tenfold. It is, therefore, important to identify their genotype and origin. Molecular genetic methods based on DNA are the most appropriate technology for this purpose. Recently, we developed an efficient marker system for the authenticity control of hop genotypes based on expressed sequence tag-simple sequence repeats (EST-SSR). In the present study, we enlarged the previously established EST-SSR set with 27 new polymorphic markers and evaluated molecular genetic variability within 135 traditional and new world hop cultivars. Two sets of 10 markers effectively differentiated all used cultivars, with the exception of cultivars derived from the same original genotype such as Saaz, Spalt, Tettnang and Nadwislawsky. Results of molecular genetic variability analyses corresponded with the genealogical and geographical origin of the key cultivars.

scholarly journals VON WILLEBRAND DISEASE: CLINICAL, COAGULOGICAL, MOLECULAR AND GENETIC DATA COMPARISON

2019 ◽  
Vol 64 (3) ◽  
pp. 246-255
Author(s):  
D. M. Chernetskaya ◽  
E. A. Likhacheva ◽  
O. S. Pshenichnikova ◽  
V. L. Surin ◽  
N. I. Zozulya
Keyword(s):  
Molecular Genetic ◽  
Genetic Data ◽  
Molecular Genetic Analysis ◽  
Disease Type ◽  
Von Willebrand Disease ◽  
World Population ◽  
Molecular Genetic Data ◽  
Major Mutation ◽  
Type 3 ◽  
Von Willebrand

Introduction. Von Willebrand disease (vWD) — one of the most common coagulopathies — is characterised by a rather complicated inheritance pattern, which can be either dominant or recessive depending on the disease type. Aim. To compare clinical, coagulological and molecular genetic data obtained when examining patients with various types of vWD.Materials and methods. The vWF gene exons were sequenced in 16 patients suffering from VWD using the Sanger method.Results. In total, 12 various mutations were identified, one of which (Pro2527His) has not been previously observed in the world population. The c.2435delC microdeletion being a major mutation in many European countries was found to be the most common. This microdeletion was observed in 9 patients, 6 of whom had the most severe recessive form of the disease — type 3 (3 homozygotes). In two patients, this disorder was accompanied by the missense mutation Thr791Met, which allowed the authors to diagnose a rather rare recessive variant of vWD — 2N. In general, the data obtained by molecular genetic analysis correlated with the differential diagnosis of the vWD type, which is based on the clinical picture of the disease and coagulological properties. In only one case, the Arg1374Cys mutation characteristic of type 2 VWD (A/M) was observed in a patient with the alleged type 1 vWD. Most of the mutations were found in exons 18 (mainly c.2435delC deletion) and 28 which makes them the most perspective exons for the mutation search.Conclusion. The search for mutations in the vWF gene should start from exons 18 and 28. The obtained information provides a basis for developing an economical algorithm aimed at searching for mutations in the vWF gene in our counrtry vWD patients. 

scholarly journals Evaluation of resistance to Pseudoperonospora humuli and of the content of alpha acids and hop oils in hops of selected genetic resources of hop Humulus lupulus L.

2021 ◽  
Author(s):  
Markéta Trefilová ◽  
Vladimír Nesvadba ◽  
Jitka Charvátová
Keyword(s):  
Genetic Resources ◽  
Drought Resistance ◽  
Acid Content ◽  
Oil Content ◽  
Humulus Lupulus ◽  
The Caucasus ◽  
Pseudoperonospora Humuli ◽  
Humulus Lupulus L ◽  
In The Wild

Twenty hop genotypes were selected for the evaluation of resistance to primary and secondary Pseudoperonospora humuli infection and of alpha acid and hop oil content in the hops. From the wild hop genotypes, two from Canada and one from Belgium showed resistance. Among the registered hop varieties, the Czech varieties Kazbek and Boomerang were the most resistant. Both wild hop genotypes from Canada showed the highest content of alpha acids among the wild hop entries, namely 4% w/w. The lowest variability of the alpha acid content in the wild hop category was found in two wild hop varieties from the Caucasus, one from Austria and one from Lithuania. The highest content of hop oils was determined in two hop genotypes from Canada and two from Belgium. Wild hop genotypes from the Caucasus have the lowest variability of hop oils among the wild hop entries. Two hop genotypes from Canada and one from Belgium were selected for breeding aimed at drought resistance.

scholarly journals Length variation of chloroplast simple sequence repeats in the genus Eucalyptus L'Hér.

2020 ◽  
Vol 7 (3) ◽  
pp. 353
Author(s):  
Sonu Kumar ◽  
Asheesh Shanker
Keyword(s):  
Simple Sequence Repeats ◽  
Molecular Breeding ◽  
Molecular Genetic ◽  
Molecular Genetic Analysis ◽  
Sufficient Information ◽  
Length Variation ◽  
The Common ◽  
Diversity Studies ◽  
Simple Sequence ◽  
Genus Eucalyptus

Eucalyptus L'Hér. is an economically important genus of plants with several environmental significances and great industrial advantages. To accelerate breeding and conservation studies, efforts on molecular breeding and molecular genetic analysis are underway in the genus Eucalyptus. Despite these efforts, no sufficient information is available about common, polymorphic and unique chloroplast simple sequence repeats (cpSSRs) in the genus Eucalyptus. . These repeats consist of 1-6 nucleotides and play important role in the development of molecular markers, genetic mapping and plant breeding. In the present study, a total of 920 cpSSRs were detected and length variation of cpSSRs analysed between each pair of species among 31 chloroplast genome sequences of the genus Eucalyptus. Additionally, cross species transferability of common and polymorphic cpSSRs were also observed. The common, unique and putative polymorphic cpSSRs analysed in this study can be used for species identification and genetic diversity studies of Eucalyptus.

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