A study of the genetic diversity in the world soybean collection using microsatellite markers associated with fungal disease resistance

Background. Soybean (Glycine max (L.) Merr.) gradually becomes one of the leading legume crops in Kazakhstan. The area under soybeans in the country has been increasing annually and requires the development of adapted cultivars with a higher yield, improved quality characters, and resistance to emerging fungal diseases. The enlargement of the crop’s gene pool also suggests the need to study and document local soybean accessions to meet the standards of the available world soybean collection by using reliable and informative types of DNA markers.Materials and methods. In this study, the soybean collection consisting of 288 accessions from different countries, including 36 cultivars and promising lines from Kazakhstan, was studied. The molecular genetic analysis was performed using nine polymorphic SSR (simple sequence repeats) markers, seven of which (Satt244, Satt565, Satt038, Satt309, Satt371, Satt570 and Sat_308) were associated with resistance to three main fungal diseases of soybean – frogeye leaf spot, fusarium root rot, and purple seed stain.Results. The average PIC (polymorphism information content) value of the analyzed SSR markers constituted 0.66 ± 0.07, confirming their highlevel polymorphism. The principal coordinate analysis suggested that the local accessions were genetically most close to the accessions from East Asia. As the collection showed a robust resistance to three studied fungal diseases in Almaty Region during 2018–2019, the distribution of the studied SSR markers in the population was not significantly associated with resistance to the analyzed diseases under field conditions.Conclusion. SSR genotyping of the soybean collection helped to identify accessions that potentially possess resistance-associated alleles of fungal disease resistance genes. The data obtained can be further used for the development of DNA documentation and the breeding the promising cultivars and lines of soybean.

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Recent Progress in Enhancing Fungal Disease Resistance in Ornamental Plants

International Journal of Molecular Sciences ◽

10.3390/ijms22157956 ◽

2021 ◽

Vol 22 (15) ◽

pp. 7956

Author(s):

Manjulatha Mekapogu ◽

Jae-A Jung ◽

Oh-Keun Kwon ◽

Myung-Suk Ahn ◽

Hyun-Young Song ◽

...

Keyword(s):

Disease Resistance ◽

Genetic Engineering ◽

Recent Progress ◽

Molecular Breeding ◽

Ornamental Plants ◽

Fungal Disease ◽

Fungal Diseases ◽

Plant Diseases ◽

Fungal Disease Resistance ◽

Ornamental Crops

Fungal diseases pose a major threat to ornamental plants, with an increasing percentage of pathogen-driven host losses. In ornamental plants, management of the majority of fungal diseases primarily depends upon chemical control methods that are often non-specific. Host basal resistance, which is deficient in many ornamental plants, plays a key role in combating diseases. Despite their economic importance, conventional and molecular breeding approaches in ornamental plants to facilitate disease resistance are lagging, and this is predominantly due to their complex genomes, limited availability of gene pools, and degree of heterozygosity. Although genetic engineering in ornamental plants offers feasible methods to overcome the intrinsic barriers of classical breeding, achievements have mainly been reported only in regard to the modification of floral attributes in ornamentals. The unavailability of transformation protocols and candidate gene resources for several ornamental crops presents an obstacle for tackling the functional studies on disease resistance. Recently, multiomics technologies, in combination with genome editing tools, have provided shortcuts to examine the molecular and genetic regulatory mechanisms underlying fungal disease resistance, ultimately leading to the subsequent advances in the development of novel cultivars with desired fungal disease-resistant traits, in ornamental crops. Although fungal diseases constitute the majority of ornamental plant diseases, a comprehensive overview of this highly important fungal disease resistance seems to be insufficient in the field of ornamental horticulture. Hence, in this review, we highlight the representative mechanisms of the fungal infection-related resistance to pathogens in plants, with a focus on ornamental crops. Recent progress in molecular breeding, genetic engineering strategies, and RNAi technologies, such as HIGS and SIGS for the enhancement of fungal disease resistance in various important ornamental crops, is also described.

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An Integrated QTL Map of Fungal Disease Resistance in Soybean (Glycine max L. Merr): A Method of Meta-Analysis for Mining R Genes

Agricultural Sciences in China ◽

10.1016/s1671-2927(09)60087-0 ◽

2010 ◽

Vol 9 (2) ◽

pp. 223-232 ◽

Cited By ~ 6

Author(s):

Jia-lin WANG ◽

Chun-yan LIU ◽

Jing WANG ◽

Zhao-ming QI ◽

Hui LI ◽

...

Keyword(s):

Disease Resistance ◽

Glycine Max ◽

Meta Analysis ◽

Fungal Disease ◽

R Genes ◽

Fungal Disease Resistance ◽

Glycine Max L ◽

Qtl Map

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THE INTEGRATED PEAR DONORS WITH MONOGENIC DETERMINED STUNT

Vestnik of the Russian agricultural science ◽

10.30850/vrsn/2018/4/17-19 ◽

1970 ◽

pp. 17-19

Author(s):

Е. А. Dolmatov ◽

Т. А. Khrykina

Keyword(s):

Disease Resistance ◽

Russian Federation ◽

Fungal Disease ◽

Winter Hardiness ◽

Adaptive Potential ◽

Research Institutes ◽

Fungal Disease Resistance ◽

Sorting Process ◽

Red Coloration

Development of low-growing varieties is one of the prioritized directions in groups selection. Solution of excessive growth in the selection can be solved in today’s conditions by two means: on a polygenic and on a monogenic level. Up until recently such work was performed by research institutes of horticulture in the U.S.S.R. and Russian Federation only on the polygenic level. The analysis is performed for the data of 17 summer studies on the development of complex donors of monogenic determined dwarfness (gene D), high winter hardiness, group fungal disease resistance (scab, leaf spot and Septoria blight) and bright red coloration of pear fruits (gene C). On the first stage of these studies the issue of the development of population of hybrid dwarf types with high adaptive potential in the conditions of the Central Black Earth region of Russia was solved based on a hybridization of the donors of high winter hardiness and fungal disease resistance with the donors of monogenic determined dwarfness which were the descendants of 4th generation of the NainVert variety. As a result, several complex donors were selected. Its use in long-term pear selection programs would make sorting process possible on earlier stages of the ontogenesis and thanks to that would make it possible to halve the size of hybrid funds. Brief description of the complex donors is given.

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Use of simple sequence repeat (SSR) markers for screening blue disease resistance in cotton germplasm exchange

AFRICAN JOURNAL OF BIOTECHNOLOGY ◽

10.5897/ajb2015.14756 ◽

2015 ◽

Vol 14 (41) ◽

pp. 2871-2875 ◽

Cited By ~ 2

Author(s):

Faustine Christopher ◽

Vieira Hoffmann Lucia ◽

Ismail Tibazarwa Flora ◽

Lukonge Everina

Keyword(s):

Disease Resistance ◽

Ssr Markers ◽

Simple Sequence Repeat ◽

Sequence Repeat ◽

Germplasm Exchange ◽

Simple Sequence

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Creation of new tomato forms with fungal disease resistance genes based on marker selection

VEGETABLE CROPS OF RUSSIA ◽

10.18619/2072-9146-2021-6-16-21 ◽

2021 ◽

pp. 16-21

Author(s):

I. N. Shamshin ◽

E. V. Grosheva ◽

M. V. Maslova ◽

R. M. Samoilova

Keyword(s):

Resistance Gene ◽

Resistance Genes ◽

Molecular Genetic ◽

Molecular Genetic Analysis ◽

Fungal Diseases ◽

Heterozygous State ◽

Standard Type ◽

Marker Selection ◽

Young Leaves ◽

Hybrid Forms

Relevance. The presented studies are aimed at obtaining new forms of tomato with a complex of genes for resistance to fungal diseases in combination with a standard type of bush and dark coloring of fruits based on marker-mediated selection.Methodology. The biological objects of the study are varieties and hybrid forms of tomato from the collection of the Michurinsky SAU. Molecular genetic analysis was performed using the following methods. DNA extraction was carried out from young leaves using a kit for isolation of NC Sample NC manufactured by Agrodiagnostika LLC according to the manufacturer's protocol. Fermentas production kits were used for PCR. Identification of the cladosporosis resistance gene was Cf-19 performed using the DNA marker R7. The presence of a fusarious wilting resistance gene was determined by a I-2/5 marker. The amplification results were visualized by agarose gel electrophoresis.Results. During the research, a collection of varieties and hybrid forms of tomato of the Michurinsky GAU was analyzed in order to identify genes for resistance to cladosporiosis Cf-19 and fusarium wilt I-2. A total of 52 genotypes were analyzed. It was found that most samples (41 samples) are characterized by a heterozygous state of the Cf-19 gene. All indeterminant and semi-determinant forms had both alleles. Of the 23 determinant forms presented in the collection, 10 had only one allele corresponding to recessive homozygote. Among all analyzed tomato genotypes, no dominant homozygous forms were noted. The study of the collection revealed several alleles of the I-2 gene. In total, four fragments corresponding to various alleles were amplified. A total of 50 resistant genotypes have been identified in the collection. Two alleys of the I-2 gene (633/693 bp) were identified in 42 tomato samples. Four varieties are homozygous in one allele (633 bp), which determines resistance. Three varieties have a second resistance allele (566 bp). One genotype has only an allele defining susceptibility (693 bp). On the basis of molecular analysis, as well as an assessment of the type of bush and fetal color, initial forms were selected with subsequent hybridization. 67 hybrid tomato plants were obtained. Evaluation of the presence of resistance genes showed that most of the resulting hybrids are resistant to cladosporiosis and fuzariosis. This is due to the presence of dominant alleles of Cf-19 and I-2 genes in a heterozygous state. Among the resulting hybrids, plants with a bark type of bush were identified. A total of 13 such plants were obtained.Conclusion. Thus, the work carried out allowed to obtain hybrid forms of tomato combine the signs of resistance to two pathogens of fungal diseases and the stem type of the bush. These forms are planned to be used in further selection work.

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QTL mapping reveals genetic determinants of fungal disease resistance in the wild lentil species Lens ervoides

Scientific Reports ◽

10.1038/s41598-017-03463-9 ◽

2017 ◽

Vol 7 (1) ◽

Cited By ~ 21

Author(s):

Vijai Bhadauria ◽

Larissa Ramsay ◽

Kirstin E. Bett ◽

Sabine Banniza

Keyword(s):

Disease Resistance ◽

Qtl Mapping ◽

Fungal Disease ◽

Genetic Determinants ◽

Fungal Disease Resistance ◽

In The Wild ◽

Wild Lentil

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Development of High-Resolution Simple Sequence Repeat Markers through Expression Profiling of Genes Associated with Pod Maturity of Soybean

Applied Sciences ◽

10.3390/app10186363 ◽

2020 ◽

Vol 10 (18) ◽

pp. 6363

Author(s):

Myoung Ryoul Park ◽

Inhye Lee ◽

Min-Jung Seo ◽

Hong-Tae Yun

Keyword(s):

High Resolution ◽

Ssr Markers ◽

Quantitative Trait ◽

Hierarchical Tree ◽

Days To Maturity ◽

Glycine Max L ◽

Late Maturity ◽

Time Required ◽

Simple Sequence ◽

Maturity Groups

In soybeans (Glycine max L.), the time required to attain maturity is a quantitative trait controlled by multiple genes and quantitative trait loci (QTL), which enable soybean cultivars to adapt to various regions with diverse day lengths. In this study, depending on the days to maturity, 100 soybean varieties were classified into eight maturity groups numbered from 0 to VII. The maturity groups were further sorted into three maturity ecotypes: early, middle, and late maturity. The analysis of 55,589 soybean genes revealed a total of 1147 related to the growth and development of soybean pods, including 211 genes with simple sequence repeats (SSRs). We further identified 42 SSR markers that amplified over two alleles in three different ecotypes, including six genes that were up- or downregulated in pods of more than one ecotype. The agglomerative hierarchical tree constructed for the newly identified SSR markers had three clusters. Clusters B-I, B-II, and B-III were found to be strongly related with the early, middle, and late maturity ecotypes, respectively. Therefore, the newly identified set of SSR markers can serve as an effective high-resolution tool for the genotyping and QTL mapping of soybean pod maturity.

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