Effects of radiation pasteurization on Salmonella. I. Parameters affecting survival and recovery from chicken

1970 ◽  
Vol 16 (6) ◽  
pp. 465-471 ◽  
Author(s):  
Joseph J. Previte ◽  
Y. Chang ◽  
H. M. El-Bisi
Keyword(s):  
Ionizing Radiation ◽  
Salmonella Typhimurium ◽  
Growth Medium ◽  
Yeast Extract ◽  
Brain Heart Infusion ◽  
Complex Media ◽  
Germ Free ◽  
Typhimurium Strain ◽  
Effects Of Radiation ◽  
Different Strains

Growth of Salmonella typhimurium, strain RIA, in minimal broth supplemented with methionine, produced a population of cells that was more sensitive to 60Co irradiation than were those grown in brain heart infusion. The resistance values (D) were 0.056 and 0.066 Mrad, respectively. D was not significantly different when the bacteria were irradiated at 4 °C on fresh or frozen-thawed germ-free chicken, or on conventional chicken sterilized by autoclaving, or by exposure to 2 Mrad of ionizing radiation. It was generally higher when the cells were irradiated in the original growth medium than on chicken. D of five different strains of S. typhimurium irradiated at 4 °C on autoclaved chicken varied from 0.052 to 0.068 Mrad. The greatest increase in resistance was produced by decreasing the temperature during irradiation. S. typhimurium, RIA, attained a D value of 0.293 Mrad at −80 °C. The effect of the recovery media on survival is equivocal. In some instances nutrient and minimal agar seemed to provide slightly better recovery of strains SR-11 and RIA. Enrichment of complex media with yeast extract did not increase recovery. The results indicate that of those parameters studied, the most significant effects on resistance were due to the temperature during irradiation.

Effects of radiation pasteurization on Salmonella. III. Radiation lethality and the frequency of mutation to antibiotic resistance

1971 ◽  
Vol 17 (3) ◽  
pp. 385-389 ◽  
Author(s):  
Joseph J. Previte ◽  
Y. Chang ◽  
H. M. El-Bisi
Keyword(s):  
Mutation Frequency ◽  
Brain Heart Infusion ◽  
Tetracycline Resistance ◽  
Processing Parameters ◽  
The Public ◽  
Typhimurium Strain ◽  
Radiation Resistant ◽  
The Difference ◽  
Public Health Hazards ◽  
Effects Of Radiation

The radiation resistance (D, in megarads) of six strains of Salmonella irradiated at 4C in brain heart infusion suspension ranged from 0.042 for S. enteritidis to 0.084 for S. thompson. The resistance values were 0.048 for S. typhimurium, strain SR-11, 0.060 for S. typhimurium, strain RIA, and S. newport, and 0.078 for S. heidelberg. The mutation frequency to tetracycline resistance of S. typhimurium, strain SR-11, increased between 0 and 0.05 Mrad and declined thereafter with increasing radiation dose. After 0.5-Mrad exposure, the mutation frequency was essentially the same as in control populations. The mutation frequency to streptomycin resistance of S. typhimurium, strain SR-11, decreased at doses greater than 0.05 Mrad and increased only slightly for the more radiation-resistant serotypes, S. typhimurium, strain RIA, S. thompson, and S. heidelberg. The average mutation frequency of the four Salmonella cultures tested was essentially unchanged (less than 1 log difference) between 0.05 and 0.5 Mrad, while the difference in reduction in viable numbers was 5 logs or greater. The evidence presented indicates that with the proper choice of processing parameters, the application of radiation pasteurization could drastically reduce the possibility of transmission of Salmonella by poultry and thereby decrease the public health hazards associated with this microorganism.

scholarly journals Genetic Control of Histidine Degradation in Salmonella typhimurium, Strain LT-2

1969 ◽  
Vol 244 (19) ◽  
pp. 5382-5391
Author(s):  
Harriet K. Meiss ◽  
Winston J. Brill ◽  
Boris Magasanik
Keyword(s):  
Salmonella Typhimurium ◽  
Genetic Control ◽  
Typhimurium Strain

Efficacy of Nisin-Coated Polymer Films To Inactivate Salmonella Typhimurium on Fresh Broiler Skin†

2000 ◽  
Vol 63 (9) ◽  
pp. 1189-1196 ◽  
Author(s):  
NANDINI NATRAJAN ◽  
BRIAN W. SHELDON
Keyword(s):  
Salmonella Typhimurium ◽  
Shelf Life ◽  
Brain Heart Infusion ◽  
Tween 80 ◽  
Linear Low Density Polyethylene ◽  
Packaging Films ◽  
Gram Positive Bacteria ◽  
Subsequent Storage ◽  
Infusion Agar ◽  
Treatment Application

Nisin is an antimicrobial peptide produced by the food-grade microorganism Lactococcus lactis subsp. lactis. This peptide inhibits many gram-positive bacteria, and when combined with chelating agents it inhibits gram-negative bacteria such as Salmonella sp. The efficacy of packaging films treated with nisin-containing formulations to reduce Salmonella contamination of fresh broiler drumstick skin and increase the refrigerated shelf life was investigated. Three films (5.1 cm2) of varying hydrophobicities (polyvinyl chloride [PVC], linear low density polyethylene, nylon) were coated with one of three liquid formulations (pH 3.5 to 3.8) composed of 100 μg/ml nisin and varying concentrations of citric acid, EDTA, and Tween 80. The treated films were applied either wet or dry to 5.1-cm2 broiler drumstick skin samples inoculated with a nalidixic acid-resistant (NAr) strain of Salmonella Typhimurium. After incubation at 4°C for 24 h the populations of surviving Salmonella TyphimuriumNAr organisms were recovered from the skin and film samples using a rinse procedure and enumerated on brain heart infusion agar containing 800 ppm NA. Log reductions (untreated versus treated skin) in Salmonella TyphimuriumNAr populations ranged from 0.4 to 2.1. Treatment formulation compositions and wet versus dry treatment application also influenced the extent of kill. The shelf life of refrigerated broiler drumsticks was extended by 0.6 to 2.2 days following a 3-min immersion in a nisin-containing treatment solution and subsequent storage in a foam tray pack containing a nisin-treated PVC overwrap and a nisin-treated absorbent tray pad. These findings demonstrated that Salmonella Typhimurium and spoilage microorganism populations on the surface of fresh broiler skin and drumsticks can be significantly reduced using immersion treatments, absorbent tray pads, and packaging films treated with nisin-containing formulations.

scholarly journals Excess of infections due to a multi-drug sensitive Salmonella enterica serotype Typhimurium in France in June 2008

2008 ◽  
Vol 13 (44) ◽  
Author(s):  
F Grandesso ◽  
N Jourdan-da Silva ◽  
S Le Hello ◽  
S Roussel ◽  
S Rasson ◽  
...  
Keyword(s):  
The Netherlands ◽  
Salmonella Typhimurium ◽  
Salmonella Enterica ◽  
Food Source ◽  
Pfge Profile ◽  
Typhimurium Strain ◽  
Specific Food

An unusually high number of cases of Salmonella Typhimurium was reported in France in June 2008. In the course of epidemiological investigations 112 cases were ascertained, of whom 75 were interviewed. Subtyping by PFGE and MLVA identified a strain named ""majority profile"". Subtyping results were available for 45 interviewed cases, 30 of whom (majority below 15 years of age) were found to be infected with the majority profile strain. Evidence suggested the occurrence of an outbreak due to a monoclonal S. Typhimurium strain with the single PFGE profile XTYM-50. Cases with identical PFGE profile were also detected in Switzerland but no link with outbreaks occurring in the same period in Denmark and in the Netherlands was found. Contamination of a product distributed nationally was suggested as the cause of the outbreak but investigations did not reveal any specific food source.

Enumeration of starter cultures in fermented milks

1996 ◽  
Vol 63 (1) ◽  
pp. 151-158 ◽  
Author(s):  
Hamid B. Ghoddusi ◽  
Richard K. Robinson
Keyword(s):  
Quality Control ◽  
Prussian Blue ◽  
Yeast Extract ◽  
Streptococcus Thermophilus ◽  
Starter Cultures ◽  
Lactobacillus Delbrueckii ◽  
Proteose Peptone ◽  
Different Strains ◽  
Differential Counts

SummarySome media available for the isolation and enumeration of starter cultures employed for the manufacture of cheese, yogurt and bio-yogurt were examined. Reddy's medium or a modification of Elliker's medium was found to be most satisfactory forLactococcusspp., while trypticase phytone yeast (TPY) agar with a mixture of antibiotics proved suitable for the discrete enumeration ofBifidobacteriumspp. The inclusion of Prussian blue (PB) in reinforced clostridial medium or tryptone proteose peptone yeast extract (TPPY) agar gave excellent differential counts for the starter bacteria in yogurt even when the culture was imbalanced, while TPPY (PB) agar allowed the visible separation of all four of the organisms that might be found in a typical bio-yogurt, namelyLactobacillus delbrueckiisubsp.bulgaricus, Streptococcus thermophilus, a,Bifidobacteriumsp. andLb. acidophilus. It was noted that variation among different strains of any given species could change the expected reactions, so for quality control purposes the suggested media may need to be modified to cope with the specific cultures in use.

Differential Immune Response of Lactobacillus plantarum 286 Against Salmonella Typhimurium Infection in Conventional and Germ-Free Mice

2020 ◽  
Author(s):  
Tizá Teles Santos ◽  
Roberta Maria Dos Santos Ornellas ◽  
Leonardo Borges Acurcio ◽  
Sávio Henrique Cicco Sandes ◽  
Andréa Miura da Costa ◽  
...  
Keyword(s):  
Immune Response ◽  
Salmonella Typhimurium ◽  
Lactobacillus Plantarum ◽  
Germ Free
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