Comparison of fatty acid composition of stable L-phase variants of Staphylococcus aureus induced by three different mechanisms

1977 ◽  
Vol 23 (10) ◽  
pp. 1478-1481 ◽  
Author(s):  
Joseph MacGee ◽  
Cheryl Glotzbecker ◽  
Chatrchai Watanakunakorn

The total saponifiable fatty acids of three stable L-phase variants of Staphylococcus aureus induced by cycloserine, methicillin, and lysostaphin were examined by gas-liquid chromatography. Five separate preparations of each of the three variants were examined. Twenty-nine fatty acids were identified. The fatty acid patterns of the three variants were very similar.

1970 ◽  
Vol 42 (4) ◽  
pp. 455-464 ◽  
Author(s):  
Md Moshfekus Saleh-E-In ◽  
Sudhangshu Kumar Roy

Anethum sowa L. (Dill) seeds were investigated to determine the fatty acid composition and proximate analyses. The seeds contain 9.36 % fatty oil. The saturated and unsaturated fatty acids contributed 6.22% and 93.78% respectively of the oil. The per cent composition of the extracted oil was identified by Gas Liquid Chromatography (GLC). Among the six fatty acids identified from this study oleic acid contributed the highest proportion (87.10%), where as, linolenic, palmitic, stearic, behenic and arachidic all together contributed the rest (12.90%). Proximate analyses showed that A. sowa. seeds are good source of dietary fibre. Overall Dill seeds oil can be considered as a good source of oleic acid. Key words: Anethum sowa, dill seed oil, fatty acid composition, oleic acid, linolenic acid, Gas liquid chromatography. Bangladesh J. Sci. Ind. Res. 42(4), 455-464, 2007


1963 ◽  
Vol 30 (3) ◽  
pp. 339-343 ◽  
Author(s):  
J. M. deMan ◽  
J. P. Bowland

SummaryAs determined by gas-liquid chromatography, the mean fatty acid composition (weight percentages of total fatty acids) of milk fat from sows fed a diet to meet U.S. N.R.C. nutrient requirements was: oleic, 35·3; palmitic, 30·3; linoleic, 13·0; palmitoleic, 9·9; stearic, 4·0; myristic, 3·3; linolenic, 2·5; unidentified 0·7 and 0·5, presumably n-odd chain and branched fatty acids; lauric, 0·3; and capric, 0·2. The corresponding fatty acid composition of colostrum fat was: oleic, 41·7; palmitic, 22·5; linoleic, 20·9; palmitoleic, 5·0; stearic, 5·7; myristic, 1·4; linolenic, 2·4; and unidentified acids, 0·3 and 0·1. Dietary fat increased fat levels in the milk and influenced fatty acid composition of the milk fat. Backfat resembled colostrum fat more than milk fat.


1962 ◽  
Vol 25 (1) ◽  
pp. 29-34 ◽  
Author(s):  
T. D. V. LAWRIE ◽  
S. G. McALPINE ◽  
R. PIRRIE ◽  
B. M. RIFKIND ◽  
JEAN BLADES

SUMMARY The fatty acid patterns of the cholesterol ester, triglyceride and phospholipid fractions of serum from thirteen hypothyroid subjects have been determined using gas-liquid chromatography. A comparison was made with the results of similar analyses performed on sixteen apparently normal subjects and on eighteen patients with ischaemic heart disease. A trend towards increasing saturation was found in each of the fractions from the hypothyroid subjects. This was most marked in the cholesterol esters. The mechanism of the production of this trend is discussed and it is suggested that it is secondary to the hyperlipidaemia of hypothyroidism. Attention is drawn to the implication of these findings in interpreting the reported changes in the fatty acid composition of the serum lipids in atherosclerosis.


1970 ◽  
Vol 24 (2) ◽  
pp. 202-208 ◽  
Author(s):  
Mohammad Mizanur Rahman ◽  
Mohammad Shahjahan

Fatty acid composition of Nyctanthes arbortristis Linn root extracted successively  with light petroleum ether (40-600C bp.)- n-hexane and chloroform were analyzed by gas liquid chromatography (GLC). Twenty one fatty acids were identified in the Seuli root. The major saturated and unsaturated fatty acids are palmitic acid (C16:0, 13.97%) and oleic acid (C18:1, 28.43%). Physio-chemical characteristics, such as iodine value, moisture, ash, lignin and crude fibre of the Seuli root were also determined.   Keywords: Nyctanthes arbortristis Linn; Seuli; root; fatty acid composition; gas liquid chromatography. DOI: http://dx.doi.org/10.3329/jbcs.v24i2.9709 Journal of Bangladesh Chemical Society, Vol. 24(2), 202-208, 2011


1965 ◽  
Vol 22 (1) ◽  
pp. 131-137 ◽  
Author(s):  
Peter M. Jangaard ◽  
R. G. Ackman

The component fatty acids of the muscle, liver, and viscera lipids from the squid, Illex illecebrosus (Le Sueur), caught in Newfoundland waters have been determined by gas–liquid chromatography (GLC). Silicic acid chromatography was used to segregate the main groups of lipids and the fatty acid composition of each fraction was determined on two organosilicone polyester columns.The lipids from the muscle consisted mainly of phospholipids with three fatty acids, palmitic (16:0), eicosapentaenoic (20:5), and docosahexaneoic (22:6) acids making up 80% of the total. The liver contained mainly triglycerides with a fatty acid composition similar to liver oils in other marine species.


1963 ◽  
Vol 46 (4) ◽  
pp. 718-725
Author(s):  
John L Iverson ◽  
David Firestone ◽  
William Horwitz

Abstract The fatty acid composition of oil extracted from 22 samples of roasted peanuts was examined by gas chromatography. The major components (> 1%) were palmitic, stearic, oleic, linoleic, arachidic, cis-11-eicosenoic (20:1), behenic (22:0), and lignoceric acids (24:0) . The identity of cis-11-eicosenoic acid was established by gas chromatography of its reduction products after ozonolysis of a sample collected from the gas chromatograph. Approximately 0.4% hexacosanoic (26:0) was present in the oils as well as trace amounts of odd chain-length saturated fatty acids (13—27 carbon atoms). Roasting the peanuts did not change the fatty acid composition of the extracted oil. The fatty acid composition of commercial peanut oils is similar to the composition of oils from roasted peanuts.


1970 ◽  
Vol 46 (1) ◽  
pp. 111-116
Author(s):  
BK Paul ◽  
MN Ahmed ◽  
GC Saha

Carum roxburghianum Benth. (Radhuni) seeds of three different places of Bangladesh were investigated to determine the fatty acid composition and physico-chemical properties of extracted oil. The seeds were found to contain oil ranging from 15.31 to 20.32%. The percentage compositions of fatty acids were identified and quantified by Gas Liquid Chromatography (GLC). The saturated and unsaturated fatty acids in extracts were 4.95 to 6.27% and 93.73 to 94.89%, respectively. Among six fatty acids identified in this study, oleic acid contributed the highest portion (76.44 to 79.16%), whereas, linoleic (1.38%), linolenic (15.28 - 15.40%), stearic (0.70%), palmitic (4.95 - 5.57%) and ecosenoic acid (0.51 - 15.73%) together contributed the rest. Physico-chemical properties of the oil extracted were also investigated. The specific gravity, refractive index, optical rotation were recorded as 0.903 to 0.918 at 30°C, 1.465 to 1.470 at 30°C and +8.54° to +9.56° at 26°C, respectively. The chemical properties like saponification value (183.52 - 193.45), iodine value (80.99 - 120.90), peroxide value (23.25 - 36.16), acid value (143.84 - 162.99), ester value (48.12 - 62.03), percentage of unsaponifiable matter (3.56 - 6.51%), Reicher-Meissel value (2.00 - 3.12), Polenske value (4.12 - 6.20) and Henher value (85.12 - 95.56) were determined. Overall Radhuni seeds oil can be considered as a good source of oleic acid. Keywords: Carum roxburghianum; Radhuni seed oil; fatty acid composition; oleic acid; Gas Liquid Chromatography. DOI: http://dx.doi.org/10.3329/bjsir.v46i1.8113 Bangladesh J. Sci. Ind. Res. 46(1), 111-116, 2011


1972 ◽  
Vol 14 (2) ◽  
pp. 247-252
Author(s):  
Christine Catchpole ◽  
R. A. Lawrie

SUMMARYThe relative concentration of the fatty acid components of the total intramuscular lipids from six muscle locations of pigs at 1·5 to 54 months of age were assessed by gas liquid chromatography. Although the pattern was generally similar at each location, differences between locations in the concentrations of oleic and arachidonic acids in particular were noted.Differences between animals in the relative concentration of fatty acids (other than linoleic) could not be accounted for by the pattern of dietary intake.


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