Efficacy and comparison of Shatapushpa Taila Matrabasti with Shatapushpa Churna in Kashtartava (Dysmenorrhoea)

Kashtartava (dysmenorrhoea) affects women’s day to day activity and it is most common complaints amongst adolescents. 75% of adolescents experience pain during menstruation. Dysmenorrhoea can also be associated with nausea, vomiting, diarrhoea, fatigue, blotting abdomen etc. As per Ayurveda Apanavata is the governing force of menstruation; its vitiation gives rise to many gynaecological diseases ( yonivyapad). Kashyapa has mentioned Shatapushpa (Anethum sowa Kurz.) as drug of choice for Artava related problems. So that group A is treated with Shatapushpa churana and group B with Shatapushpa taila matra basti. Shatapushpa Churna and Shatapushpa taila matrabasti showed extremely significant (P< 0.0001) relief in severity of pain however showed significant relief in duration of Pain, Nausea and Breast Tenderness. Overall effect of treatment on Group A showed complete remission 27.27% whereas Group B showed 63.64%. Statistically both groups are expressed significant results, whereas on the basis of clinical symptoms and overall effect of treatment illustrated in the study the Shatapushpa taila matrabasti is more effective than Shatapushpa churna.

Anticancer, Anticollagenase and in Silico Docking Studies of Anethum sowa L. Herb Oil against HCT 116 Human Colorectal Cancer Cell Line

Aim: Anethum sowa L. isan aromatic plant with pharmacological potential. The chemical composition and the therapeutic of Anethum sowa L. herb oil grown in South Karnataka is very few; moreover, its essential oil and extract together is not being studied and compared for its effects on colon cancer cell lines HCT -116 and anti collagense study . Methods: The current investigation was intended to sight see the incidence of components present in the herb oil examined by (GC-MS), antioxidant , antimicrobial , anticancer & anticollagenase potential was investigated and further the insilco docking studies to unleash the potential drug like molecules in the therapeutic plant was studied . Results: 5-Oxo-4,5,6,7-tetrahydro-1H-pyrrolo[2,3-c]pyridine-3-propionic acid methyl ester, (17.41%),beta-Amyrin(8.20),ritodrine(6.49),1-Naphthalenol,decahydro-1,4a-dimethyl-7-(methylethylidene)-,[1R-(1.alpha.4a.beta,8a.alpha)](2.39%),meta-Cymene(1.95%),trans-z-alpha-Bisabolene epoxide (1.80), and Viridiflorol(0.77%) were the new compounds isolated from this therapeutic plant, and Anethum sowa L. herb ethanolic extract contained many potential phytochemicals. The total phenol and flavonoid of the herb extract were 0.136mg/ml. and 0.108mg/ml respectively . Anethum sowa L. herb extract ABTS antioxidant assay showed excellent activity with an IC50 of 540µg/ml which was in power with gallic acid which showed an IC 50 of 393µg/ml. Essential oil of Anethum sowa L. herb exhibited potent antimicrobial activity against all the three microorganisms E-coli strain (MTCC 433), Klebsiella pneumoniae strain (MTCC 3384) Streptococcusmutants strain (MTCC 497) with a minimum inhibitory concentration of 20% herb oil . Cytotoxicity of Anethum sowa L.herb essential oil and ethanolic extract against Colon cancer cell lines – HCT -116 , showed that herb oil and herb ethanolic extract repressed the cell growth of the cell . Herb oil with an IC50 79.75µg/ml was more effective than the herb extract . Herb essential oil showed the maximum capacity in inhibiting the collagenase when compared to ethanolic extract of herb, the percentage of inhibition of Anethum sowa L. herb essential oil was found to be 60.89% and that of herb extract was (15.18%). Conclusion: Herb oil showed very good anticancer, antimicrobial and anticollagenase activity and by the In silico docking performed between the compounds present in the herb oil, MAI-150 and APC of Homo sapiens, it was found that lupeol showed the highest binding affinity with APC when compared with MAI-150 and rest other compounds present in the herb oil.

Shatapushpa (Anethum sowa) Churna in the management of Artavakshaya W.S.R to Hypomenorrhoea: A case study

Normal menstrual cycle is an important physiology for maintaining woman’s health status during reproductive stage. Any type of abnormality in normal Rituchakra (menstrual cycle) cause menstrual disorders, which are the main reasons for gynaecological consultations in worldwide. Hypomenrrhoea is a common menstrual disorder found in present era due to changes in their life style like restlessness due to stressful day to day routine and strain due to their work place environment. A 24 years old Muslim married (since 2 years) patient present with the complaints of scanty menstrual flow (Duration-6days, No. of pad used-2pad 1st day not fully soaked then 1 pad/day not fully soaked) and lower abdominal pain (++) with pain and burning sensation during coitus for 1 year. In Ayurveda it can be correlated with Artavakshaya due to their strong symptom equality. Ratio of Artavakshaya is rising in present gynaecological practice which may leads to cause infertility and other serious problems which are untreatable. In modern science they prescribe only hormonal preparations as a treatment. Many patients observe some adverse effects with reoccurrence of problem when hormonal preparation stops so they prefer to take Ayurvedic preparation for it. Hence, Shatapushpa Churna is selected for the case study which has Agneya properties, Kapha Shamaka & Aacharya Kashyapa also quote it as Ritupravritani.

STUDY OF SHATAPUSHPA CHOORNA AND SAHACHARADI TAILA IN OLIGOMENORRHOEA

The abnormal menstrual flow indicates heavy menses and scanty menstrual flow, both in amount and duration with associated symptom of pain in lower abdomen or back or vagina and can be counted as Vataja Rajodushti, Kshinartava and Artavakshaya in Ayurveda classics. Aims and objectives: In Ayurvedic classic, Uttarabasti is indicated in Yonivyapada (gynecological problems), Artava nasha (secondary amenorrhea), Artava Dosha (menstrual disorder) etc. Vatadosha is the prime cause of Yoni vyapada. Uttara basti has Vatashamak as well as Ropana and Shodhana property. Sahacharadi taila is indicated in Vata disorders including oligomenorrhea. Shatapushpa (Anethum sowa Kurz.) Churna is Arthavajanan. Materials and methods: From OPD 30 patients were selected randomly and divided into 15 patients in each Group A and Group B. Group A was given Shatapushpa churna orally 3times per day for 3 months and Sahacharadi taila Uttaravasti for 3 consecutive cycles. Group B was given placebo. Results were analysed by statistical analysis. Results and conclusion: Properties of Shatapushpa churna, Saacharadi taila with Uttarbasti might improve menstrual irregularities and bring down to normalcy.

Thrombolytic, Membrane Stabilizing and Hypoglycemic Activities of Anethum sowa Linn.

The hexane (HE), dichloromethane (DCME), ethyl acetate (EAE) and methanol (ME) extracts of seed and stem of Anethum sowa were subjected to screenings for thrombolytic, membrane stabilizing and hypoglycemic activities. Ethyl acetate extract of stem showed highest thrombolytic activity. In addition, the dichloromethane, ethyl acetate and methanol extract of seed revealed higher percentage (%) of inhibition in hypotonic solution induced hemolysis. In hypoglycemic activity, the dichloromethane and ethyl acetate extracts of stem and seed displayed significant blood glucose lowering effect. Dhaka Univ. J. Pharm. Sci. 17(2): 237-241, 2018 (December)