Thiobacillus acidophilus: a study of its presence in Thiobacillus ferrooxidans cultures

1980 ◽  
Vol 26 (9) ◽  
pp. 1057-1065 ◽  
Author(s):  
G. J. M. W. Arkesteyn ◽  
J. A. M. de Bont
Keyword(s):  
Amino Acids ◽  
Organic Acids ◽  
Organic Compounds ◽  
Ferrous Iron ◽  
Thiobacillus Ferrooxidans ◽  
Mixed Cultures ◽  
Limited Extent ◽  
Order Of Magnitude ◽  
Thiobacillus Acidophilus

A study had been undertaken to account for the presence of Thiobacillus acidophilus in iron-grown cultures of Thiobacillus ferrooxidans. Attempts to adapt T. acidophilus to ferrous iron were not successful but the facultative autotroph grew to a limited extent in the spent medium of T. ferrooxidans and was able to grow oligotrophically. Possible oligotrophic substrates were methanol, ethanol, and sulphide. Thiobacillus ferrooxidans may benefit from the presence of T. acidophilus because in mixed cultures some inhibiting organic compounds such as alcohols, organic acids, and amino acids were utilized by T. acidophilus. The number of T. acidophilus cells in heterogeneous cultures with T. ferrooxidans was of the same order of magnitude as the number of T. ferrooxidans cells as revealed by fluorescent-labelled antibodies.

Leaching of Pyrite by Acidophilic Heterotrophic Iron-Oxidizing Bacteria in Pure and Mixed Cultures

1999 ◽  
Vol 65 (2) ◽  
pp. 585-590 ◽  
Author(s):  
Paula Bacelar-Nicolau ◽  
D. Barrie Johnson
Keyword(s):  
Yeast Extract ◽  
Thiobacillus Ferrooxidans ◽  
Pyrite Oxidation ◽  
Mixed Cultures ◽  
Mineral Dissolution ◽  
Acidophilic Bacteria ◽  
Pure Cultures ◽  
Sulfur Oxidizing ◽  
Thiobacillus Acidophilus ◽  
Basal Salts

ABSTRACT Seven strains of heterotrophic iron-oxidizing acidophilic bacteria were examined to determine their abilities to promote oxidative dissolution of pyrite (FeS2) when they were grown in pure cultures and in mixed cultures with sulfur-oxidizingThiobacillus spp. Only one of the isolates (strain T-24) oxidized pyrite when it was grown in pyrite-basal salts medium. However, when pyrite-containing cultures were supplemented with 0.02% (wt/vol) yeast extract, most of the isolates oxidized pyrite, and one (strain T-24) promoted rates of mineral dissolution similar to the rates observed with the iron-oxidizing autotroph Thiobacillus ferrooxidans. Pyrite oxidation by another isolate (strain T-21) occurred in cultures containing between 0.005 and 0.05% (wt/vol) yeast extract but was completely inhibited in cultures containing 0.5% yeast extract. Ferrous iron was also needed for mineral dissolution by the iron-oxidizing heterotrophs, indicating that these organisms oxidize pyrite via the “indirect” mechanism. Mixed cultures of three isolates (strains T-21, T-23, and T-24) and the sulfur-oxidizing autotroph Thiobacillus thiooxidans promoted pyrite dissolution; since neither strains T-21 and T-23 nor T. thiooxidans could oxidize this mineral in yeast extract-free media, this was a novel example of bacterial synergism. Mixed cultures of strains T-21 and T-23 and the sulfur-oxidizing mixotrophThiobacillus acidophilus also oxidized pyrite but to a lesser extent than did mixed cultures containing T. thiooxidans. Pyrite leaching by strain T-23 grown in an organic compound-rich medium and incubated either shaken or unshaken was also assessed. The potential environmental significance of iron-oxidizing heterotrophs in accelerating pyrite oxidation is discussed.

Photosynthetic assimilation and translocation of 3H- and 14C-organic compounds after 3HHO and 14CO2 were simultaneously offered to a primary leaf of soybean

1971 ◽  
Vol 49 (5) ◽  
pp. 757-766 ◽  
Author(s):  
Robert G. Thompson ◽  
C. D. Nelsons
Keyword(s):  
Amino Acids ◽  
Glycine Max ◽  
Organic Acids ◽  
Glutamic Acid ◽  
Organic Compounds ◽  
Primary Leaf ◽  
Main Compound ◽  
Glycine Max L ◽  
Photosynthetic Assimilation

After assimilation of both 3HHO and 14CO2 by soybean (Glycine max L.) in the light, the percentage of nonexchangeable 3H was similar to the percentage of 14C distributed in each of the identified sugars, amino acids, and organic acids with the exceptions of glycine–serine and glutamic acids. Glycine–serine contained proportionally more 14C than expected on the basis of percentage of 3H; and glutamic acid contained proportionally more 3H than expected on the basis of 14C. Both 3H- and 14C-organic compounds were translocated in the same proportions to various parts of the plant and in both cases the main compound translocated was apparently sucrose. The metabolism of dark-assimilated 3H was different from dark-assimilated 14C, but in both cases it did not contribute significantly to the total metabolism of the plant.

scholarly journals Comparative Investigation of Combined Metabolomics-Flavoromics during the Ripening of Mango (Mangifera indica L.) cv. ‘Nam Dok Mai Si Thong’ and ‘Nam Dok Mai No. 4’

Plants ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2198
Author(s):  
Ye Lin Lin Aung ◽  
Yaowapa Lorjaroenphon ◽  
Pinthip Rumpagaporn ◽  
Sudathip Sae-tan ◽  
Kriskamol Na Na Jom
Keyword(s):  
Amino Acids ◽  
Volatile Organic Compounds ◽  
Organic Acids ◽  
Organic Compounds ◽  
Malic Acid ◽  
Mangifera Indica ◽  
Comparative Investigation ◽  
Volatile Organic ◽  
Ethyl Octanoate ◽  
Ethyl Decanoate

A metabolomics-flavoromics approach was conducted to assess the micromolecules of ‘Nam Dok Mai Si Thong’ and ‘Nam Dok Mai No. 4’ mango cultivars from two seasons. During ripening, FAMEs, FFAs, fatty alcohols, sterols, and organic acids were dominant at 0–2 days, whereas amino acids, sugars, and volatile organic compounds, including esters, alcohols, ketones, aldehydes, and terpenes, were at higher levels at 4–8 days. Nine metabolites (palmitic/linoleic/linolenic/citric/malic acids, β-sitosterol, sucrose, glycine, and leucine) and two volatile organic compounds (ethyl octanoate/decanoate) were related to ripening-associated changes within eight days. During ripening, sucrose at 6–8 days, citric/malic acid at 0–2 days, glycine and leucine at 4 days, and ethyl octanoate and ethyl decanoate at 8 days could be used as quality biomarkers for Nam Dok Mai Si Thong; palmitic/linoleic/linolenic acids at 0 days and β-sitosterol at 0–4 days could be used as quality biomarkers for Nam Dok Mai No. 4.

Inhibition of growth, iron, and sulfur oxidation in Thiobacillus ferrooxidans by simple organic compounds

1976 ◽  
Vol 22 (5) ◽  
pp. 719-730 ◽  
Author(s):  
Jon H. Tuttle ◽  
Patrick R. Dugan
Keyword(s):  
Organic Compounds ◽  
Ferrous Iron ◽  
Thiobacillus Ferrooxidans ◽  
Cell Envelope ◽  
Iron Oxidation ◽  
Inorganic Ions ◽  
Sulfur Oxidation ◽  
Physical Treatment ◽  
Inhibition Of Growth ◽  
Iron And Sulfur Oxidation

Iron and sulfur oxidation by Thiobacillus ferrooxidans as well as growth on ferrous iron were inhibited by a variety of low molecular weight organic compounds. The influences of chemical structure of the organic inhibitors, pH, temperature, physical treatment of cells, and added inhibitory or stimulatory inorganic ions and iron oxidation suggest that a major factor contributing to the inhibitory effects on iron oxidation is the relative electronegativity of the organic molecule. The data also suggest that inhibitory organic compounds may (i) directly affect the iron-oxidizing enzyme system, (ii) react abiologically with ferrous iron outside the cell, (iii) interfere with the roles of phosphate and sulfate in iron oxidation, and (iv) nonselectively disrupt the cell envelope or membrane.

scholarly journals Water Stress- and Nutrient Solution-mediated Changes in Water Relations and Amino Acids, Organic Acids, and Sugars in Xylem Fluid of Prunus salicina and Lagerstroemia indica

1995 ◽  
Vol 120 (1) ◽  
pp. 36-42 ◽  
Author(s):  
Peter C. Andersen ◽  
Brent V. Brodbeck ◽  
Russell F. Mizell
Keyword(s):  
Amino Acids ◽  
Water Stress ◽  
Organic Acids ◽  
Organic Compounds ◽  
Water Relations ◽  
Nutrient Solution ◽  
Plant Water Relations ◽  
Xylem Fluid ◽  
Prunus Salicina ◽  
Organic Acids And Sugars

The effects and interactions of water stress and nutrient solution on water relations and concentrations of amino acids, organic acids and sugars in xylem fluid of `Methley' plum (Prunus salicina Lindl.) and `Carolina Beauty' crape myrtle (Lagerstroemia indica L.) during midday were determined. Container-grown plants were irrigated with water or nutrient solution (i.e., osmolarity = 138 mm) for 15 days, then irrigation was either continued or terminated for the next 5 days. The experiments were analyzed as factorial designs for each species separately, with the nutrient solution and irrigation status the last 5 days as the main factors. Xylem fluid tension increased ≈ 2- to 3-fold and leaf conductance to water vapor and transpiration were reduced ≈ 10-fold by withholding irrigation for both species; plant water relations of L. indica were also influenced by the nutrient solution. For both species, the osmolarity of xylem fluid was not altered by withholding irrigation. The predominant organic compounds quantified in both species were amides (i.e., glutamine and asparagine), arginine, and citric and malic acids. Sugars represented a small proportion (i.e., generally ≤ 1%) of total osmolarity. Irrigation altered the chemical profile of amino acids and organic acids to a greater degree than the nutrient solution. Water stress induced a 3-fold increase in total organic acids in xylem fluid of both species. The osmolarity and the concentration of most organic compounds in xylem fluid of P. salicina were not significantly affected by the nutrient solution. Arginine increased markedly in concentration by withholding irrigation or with the application of nutrient solution for L. indica. The concentration of most organic compounds did not vary greatly in response to variations in soil water or nutrient status. In conclusion, soil water-or nutrient-mediated changes in plant water relations exceeded changes in xylem fluid chemistry.

Synthesis and Breakdown of the Universal Precursors to Biological Metabolism Promoted by Ferrous Iron

2018 ◽  
Author(s):  
Kamila B. Muchowska ◽  
Sreejith Jayasree VARMA ◽  
Joseph Moran
Keyword(s):  
Amino Acids ◽  
Chemical Reaction ◽  
Metabolic Pathways ◽  
Ferrous Iron ◽  
Reaction Network ◽  
Tca Cycle ◽  
Chemical Reaction Network ◽  
Metabolic Precursors ◽  
Tca Cycle Intermediates

How core biological metabolism initiated and why it uses the intermediates, reactions and pathways that it does remains unclear. Life builds its molecules from CO<sub>2 </sub>and breaks them down to CO<sub>2 </sub>again through the intermediacy of just five metabolites that act as the hubs of biochemistry. Here, we describe a purely chemical reaction network promoted by Fe<sup>2+ </sup>in which aqueous pyruvate and glyoxylate, two products of abiotic CO<sub>2 </sub>reduction, build up nine of the eleven TCA cycle intermediates, including all five universal metabolic precursors. The intermediates simultaneously break down to CO<sub>2 </sub>in a life-like regime resembling biological anabolism and catabolism. Introduction of hydroxylamine and Fe<sup>0 </sup>produces four biological amino acids. The network significantly overlaps the TCA/rTCA and glyoxylate cycles and may represent a prebiotic precursor to these core metabolic pathways.

scholarly journals Profiles of Secondary Metabolites (Phenolic Acids, Carotenoids, Anthocyanins, and Galantamine) and Primary Metabolites (Carbohydrates, Amino Acids, and Organic Acids) during Flower Development in Lycoris radiata

Biomolecules ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 248
Author(s):  
Chang Ha Park ◽  
Hyeon Ji Yeo ◽  
Ye Jin Kim ◽  
Bao Van Nguyen ◽  
Ye Eun Park ◽  
...  
Keyword(s):  
Amino Acids ◽  
Secondary Metabolites ◽  
Organic Acids ◽  
Phenolic Acids ◽  
Flower Development ◽  
Developmental Stages ◽  
Tca Cycle ◽  
Primary And Secondary Metabolites ◽  
Hydrophilic Compounds ◽  
Tca Cycle Intermediates

This study aimed to elucidate the variations in primary and secondary metabolites during Lycorisradiata flower development using high performance liquid chromatography (HPLC) and gas chromatography time-of-flight mass spectrometry (GC-TOFMS). The result showed that seven carotenoids, seven phenolic acids, three anthocyanins, and galantamine were identified in the L. radiata flowers. Most secondary metabolite levels gradually decreased according to the flower developmental stages. A total of 51 metabolites, including amines, sugars, sugar intermediates, sugar alcohols, amino acids, organic acids, phenolic acids, and tricarboxylic acid (TCA) cycle intermediates, were identified and quantified using GC-TOFMS. Among the hydrophilic compounds, most amino acids increased during flower development; in contrast, TCA cycle intermediates and sugars decreased. In particular, glutamine, asparagine, glutamic acid, and aspartic acid, which represent the main inter- and intracellular nitrogen carriers, were positively correlated with the other amino acids and were negatively correlated with the TCA cycle intermediates. Furthermore, quantitation data of the 51 hydrophilic compounds were subjected to partial least-squares discriminant analyses (PLS-DA) to assess significant differences in the metabolites of L. radiata flowers from stages 1 to 4. Therefore, this study will serve as the foundation for a biochemical approach to understand both primary and secondary metabolism in L. radiata flower development.

scholarly journals Metabolomic-Based Comparison of Traditional and Industrial Doenjang Samples with Antioxidative Activities

Foods ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1377
Author(s):  
Song-Hui Soung ◽  
Sunmin Lee ◽  
Seung-Hwa Lee ◽  
Hae-Jin Kim ◽  
Na-Rae Lee ◽  
...  
Keyword(s):  
Amino Acids ◽  
Statistical Analysis ◽  
Organic Acids ◽  
Biogenic Amines ◽  
Multivariate Statistical Analysis ◽  
Metabolite Profiling ◽  
New Products ◽  
Multivariate Statistical ◽  
Fermentation Processes

Numerous varieties of doenjang are manufactured by many food companies using different ingredients and fermentation processes, and thus, the qualities such as taste and flavor are very different. Therefore, in this study, we compared many products, specifically, 19 traditional doenjang (TD) and 17 industrial doenjang (ID). Subsequently, we performed non-targeted metabolite profiling, and multivariate statistical analysis to discover distinct metabolites in two types of doenjang. Amino acids, organic acids, isoflavone aglycones, non-DDMP (2,3-dihydro-2,5-dihydroxy-6-methyl-4H-pyran-4- one) soyasaponins, hydroxyisoflavones, and biogenic amines were relatively abundant in TD. On the contrary, contents of dipeptides, lysophospholipids, isoflavone glucosides and DDMP-conjugated soyasaponin, precursors of the above-mentioned metabolites, were comparatively higher in ID. We also observed relatively higher antioxidant, protease, and β-glucosidase activities in TD. Our results may provide valuable information on doenjang to consumers and manufacturers, which can be used while selecting and developing new products.

The Xylem Sap of Maize Roots: Its Collection, Composition and Formation

1988 ◽  
Vol 15 (4) ◽  
pp. 557 ◽  
Author(s):  
MJ Canny ◽  
ME Mccully
Keyword(s):  
Amino Acids ◽  
Organic Acids ◽  
Organic Acid ◽  
Aspartic Acid ◽  
Xylem Sap ◽  
Great Variability ◽  
Reduced Pressure ◽  
Transpiration Stream ◽  
Maize Roots ◽  
Total Amino Acids

Three methods of sampling xylem sap of maize roots were compared: sap bleeding from the stem cut just above the ground; sap bleeding from the cut tops of roots still undisturbed in the ground; and sap aspirated from excavated roots under reduced pressure. The bleeding saps were often unobtainable. When their composition was measured with time from cutting, the concentrations of the major solutes approximately doubled in 2 h. Aspirated sap was chosen as the most reliable sample of root xylem contents. Solute concentrations of the saps showed great variability between individual roots for all solutes, but on average the concentrations found (in �mol g-1 sap) were: total amino acids, 1.8; nitrate, 1.8; sugars (mainly sucrose), 5.4; total organic acids, 18.3. Individual amino acids also varied greatly between roots. Glutamine, aspartic acid and serine were generally most abundant. The principal organic acid found was malic, approximately 8 �mol g-1. From these analyses the ratios of carbon in the fractions (sugars : amino acids : organic acids) = (44 : 6 : 50). 14Carbon pulse fed to a leaf appeared in the root sap within 30 min, rose to a peak at 4-6 h, and declined slowly over a week. During all this time the neutral, cation and anion fractions were sensibly constant in the proportions 86 : 10 : 4. The 14C therefore did not move towards the equilibrium of 12C-compounds in the sap. It is argued that the results do not support a hypothesis of formation of amino carbon from recent assimilate and reduced nitrate in the roots and an export of this to the shoot in the transpiration stream.

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