Characterization of the surface antigens of the marine fish pathogens Vibrio anguillarum and Vibrio ordalii

1984 ◽  
Vol 30 (5) ◽  
pp. 703-710 ◽  
Author(s):  
Henrik Chart ◽  
Trevor J. Trust
Keyword(s):  
Outer Membrane ◽  
Chain Length ◽  
Extraction Procedure ◽  
Vibrio Anguillarum ◽  
Surface Antigens ◽  
Cross Reactivity ◽  
Antigenic Specificity ◽  
Fish Pathogens ◽  
Morphological Heterogeneity ◽  
Vibrio Ordalii

The technique of immunoblotting was used to identify the surface antigens of the marine vibrios pathogenic for fish, Vibrio anguillarum and Vibrio ordalii. Polyclonal antisera raised in rabbits to strains representing the two most common serotypes causing Vibriosis in fish in North America were used. The results demonstrated that antigenic specificity was conferred by the lipopolysaccharides, with three serotypes being displayed among the strains examined. The lipopolysaccharides of strains chosen as type species for V. anguillarum and V. ordalii displayed antigenic cross-reactivity. The morphological heterogeneity of the Vibrio lipopolysaccharides was also analyzed in silver-stained polyacrylamide gels and by intrinsic 32P-radiolabelling. Two distinct lipopolysaccharide morphologies were exhibited, one with 0 polysaccharide chains of heterogeneous chain length, the other having 0 polysaccharide chains of more uniform chain length but displaying microheterogeneity. These lipopolysaccharide morphologies corresponded to different serogroups. Two minor proteins of apparent molecular weights 49 000 – 51 000 present in outer membrane preparations isolated by the sarcosinate extraction procedure were also strong antigens, and common to all strains of V. anguillarum tested and to several strains of V. ordalii. The major outer membrane protein was a weak antigen common to both species.

Antibody specificities of polyclonal rabbit and rainbow trout antisera against Vibrio ordalii and serotype 0:2 strains of Vibrio anguillarum

1993 ◽  
Vol 39 (5) ◽  
pp. 492-499 ◽  
Author(s):  
Lucy W. Mutharia ◽  
Bonnie T. Raymond ◽  
Teri R. Dekievit ◽  
Roselynn M. W. Stevenson
Keyword(s):  
Rainbow Trout ◽  
Vibrio Anguillarum ◽  
Immunoblot Analysis ◽  
Cross Reactivity ◽  
Antigenic Determinants ◽  
Polyclonal Rabbit ◽  
Common Antigens ◽  
Antibody Specificities ◽  
O Antigens ◽  
Vibrio Ordalii

Polyclonal rabbit antisera raised against Vibrio ordalii and serotype 02 strains of Vibrio anguillarum showed extensive cross-reactivity with lipopolysaccharide from these bacterial pathogens of fish when tested in western immunoblot analysis. Results with absorbed polyclonal antisera indicated that lipopolysaccharide molecules from these strains had both common and strain-specific antigenic determinants, which allowed the antisera to be used to differentiate between V. ordalii and serotype 02 strains of V. anguillarum. Unlike rabbits, the immune response in rainbow trout to serotype 02 common antigenic epitopes was dependent on the source of the immunizing lipopolysaccharide antigens. Serum from fish immunized with V. ordalii antigens reacted more extensively with serotype 02 common antigens. In contrast, fish anti-V. anguillarum 02 serum did not interact with O antigens from the V. ordalii strains. Lipopolysaccharide from V. anguillarum serotype 02 and 02a strains showed identical antibody binding properties when interacted with rabbit or fish antiserum to either V. anguillarum 02 or V. ordalii. Lipopolysaccharide from V. anguillarum 02b strains did not interact with the tested rabbit or fish polyclonal sera. The results from this study suggest that fish and rabbits recognise different antigenic determinants in lipopolysaccharide from V. ordalii and serotpye 02 V. anguillarum strains; that V. ordalii and serotype 02 strains of V. anguillarum should be regarded as distinct serotype 02 subgroups based on the strain-specific antigenic determinants; and finally that the serological classification of V. anguillarum serotype 02b strains should be reexamined.Key words: antigenic heterogeneity, lipopolysaccharides, fish immune response.not available

scholarly journals FrpA is the outer membrane piscibactin transporter in Vibrio anguillarum: structural elements in synthetic piscibactin analogues required for transport

2021 ◽  
Author(s):  
Marta A. Lages ◽  
M. Carmen de la Fuente ◽  
Lucía Ageitos ◽  
Diana Martínez-Matamoros ◽  
Jaime Rodríguez ◽  
...  
Keyword(s):  
Outer Membrane ◽  
Vibrio Anguillarum ◽  
Hydroxyl Group ◽  
Thiazole Ring ◽  
Antimicrobial Compounds ◽  
Structural Elements ◽  
Fish Pathogens ◽  
Major Virulence Factor ◽  
Photobacterium Damselae ◽  
Outer Membrane Transporter

AbstractPiscibactin (Pcb) is a labile siderophore widespread among Vibrionaceae. Its production is a major virulence factor of some fish pathogens such as Photobacterium damselae subsp. piscicida and Vibrio anguillarum. Although FrpA was previously suggested as the putative outer membrane transporter (OMT) for ferri-piscibactin, its role in piscibactin uptake was never demonstrated. In this work, we generated mutants of V. anguillarum defective in FrpA and analyzed their ability to use piscibactin as iron source. The results showed that inactivation of frpA completely disables piscibactin utilization, and the original phenotype could be restored by gene complementation, confirming that FrpA is the OMT that mediates ferri-Pcb uptake. Additionally, the ability of several Pcb thiazole analogues, with different configurations at positions 9, 10, and 13, to be internalized through FrpA, was evaluated measuring their ability to promote growth under iron deficiency of several indicator strains. The results showed that while those analogues with a thiazole ring maintain almost the same activity as Pcb, the maintenance of the hydroxyl group present in natural piscibactin configuration at position C-13 is crucial for Fe3+ chelation and, in consequence, for the recognition of the ferri-siderophore by the cognate OMT. All these findings allowed us to propose a Pcb analogue as a good candidate to vectorize antimicrobial compounds, through the Trojan horse strategy, to develop novel compounds against bacterial fish diseases. Graphical abstract

Ultraviolet Treatment of Water for Destruction of Five Gram-Negative Bacteria Pathogenic to Fishes

1977 ◽  
Vol 34 (8) ◽  
pp. 1244-1249 ◽  
Author(s):  
G. L. Bullock ◽  
H. M. Stuckey
Keyword(s):  
Particulate Matter ◽  
Ultraviolet Irradiation ◽  
Vibrio Anguillarum ◽  
Spring Water ◽  
Aeromonas Salmonicida ◽  
Water Disinfection ◽  
Gram Negative Bacteria ◽  
Fish Pathogens ◽  
Gram Negative ◽  
Ultraviolet Treatment

Filtration (25 nm) and ultraviolet irradiation dosages of 13,100–29,400 microwatt seconds per square centimetre (μW∙s∙cm−2) effected a 99.98–100% reduction of five gram-negative fish pathogens — Aeromonas salmonicida, A. hydrophila, Vibrio anguillarum, Pseudomonas fluorescens, and the enteric redmouth organism in 12.5 °C clear spring water or spring water containing particulate matter. Filtration and a dosage of 4500 μW∙s∙cm−2 killed 99.83–100% of test strains in spring water and 4000–4750 μW∙s∙cm−2 killed 99.33–99.99% in water with particulate matter. Irradiation of unfiltered water containing particulate matter was less effective, especially at dosages of 5000 μW∙s∙cm−2 or less, which killed 97–99.94% of strains. Filtration and 13,100 μW∙s∙cm−2 irradiation of water containing A. salmonicida prevented transmission of furunculosis. Key words: ultraviolet irradiation, bacterial fish pathogens, water disinfection

scholarly journals The Fish Pathogen Vibrio ordalii Under Iron Deprivation Produces the Siderophore Piscibactin

2019 ◽  
Vol 7 (9) ◽  
pp. 313 ◽  
Author(s):  
Pamela Ruiz ◽  
Miguel Balado ◽  
Juan Carlos Fuentes-Monteverde ◽  
Alicia E. Toranzo ◽  
Jaime Rodríguez ◽  
...  
Keyword(s):  
Gene Cluster ◽  
Iron Uptake ◽  
Peptide Mass Fingerprinting ◽  
Iron Limitation ◽  
Fish Pathogen ◽  
Fish Pathogens ◽  
Iron Deprivation ◽  
Peptide Mass ◽  
Photobacterium Damselae ◽  
Vibrio Ordalii

Vibrio ordalii is the causative agent of vibriosis, mainly in salmonid fishes, and its virulence mechanisms are still not completely understood. In previous works we demonstrated that V. ordalii possess several iron uptake mechanisms based on heme utilization and siderophore production. The aim of the present work was to confirm the production and utilization of piscibactin as a siderophore by V. ordalii. Using genetic analysis, identification by peptide mass fingerprinting (PMF) of iron-regulated membrane proteins and chemical identification by LC-HRMS, we were able to clearly demonstrate that V. ordalii produces piscibactin under iron limitation. The synthesis and transport of this siderophore is encoded by a chromosomal gene cluster homologous to another one described in V. anguillarum, which also encodes the synthesis of piscibactin. Using β-galactosidase assays we were able to show that two potential promoters regulated by iron control the transcription of this gene cluster in V. ordalii. Moreover, biosynthetic and transport proteins corresponding to piscibactin synthesis and uptake could be identified in membrane fractions of V. ordalii cells grown under iron limitation. The synthesis of piscibactin was previously reported in other fish pathogens like Photobacterium damselae subsp. piscicida and V. anguillarum, which highlights the importance of this siderophore as a key virulence factor in Vibrionaceae bacteria infecting poikilothermic animals.

Xanthan chain length is modulated by increasing the availability of the polysaccharide copolymerase protein GumC and the outer membrane polysaccharide export protein GumB

Glycobiology ◽  
2012 ◽  
Vol 23 (2) ◽  
pp. 259-272 ◽  
Author(s):  
Estela M Galván ◽  
María V Ielmini ◽  
Yamini N Patel ◽  
María I Bianco ◽  
Esteban A Franceschini ◽  
...  
Keyword(s):  
Outer Membrane ◽  
Chain Length ◽  
Export Protein

Structure of the O-chain polysaccharide of the phenol-phase soluble lipopolysaccharide of Escherichia coli 0:157:H7

1986 ◽  
Vol 64 (1) ◽  
pp. 21-28 ◽  
Author(s):  
Malcolm B. Perry ◽  
Leann MacLean ◽  
Douglas W. Griffith
Keyword(s):  
Escherichia Coli ◽  
Brucella Abortus ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Extraction Procedure ◽  
Periodate Oxidation ◽  
Cross Reactivity ◽  
E Coli ◽  
Structure Formula ◽  
Phenol Water

The phenol-phase soluble lipopolysaccharide isolated from Escherichia coli 0:157 by the hot phenol–water extraction procedure was shown by sodium dodecyl sulfate–polyacrylamide gel electrophoresis, periodate oxidation, methylation, and 13C and 1H nuclear magnetic resonance studies to be an unbranched linear polysaccharide with a tetrasaccharide repeating unit having the structure:[Formula: see text]The serological cross-reactivity of E. coli 0:157 with Brucella abortus, Yersinia enterocolitica (serotype 0:9), group N Salmonella, and some other E. coli species can be related immunochemically to the presence of 1,2-glycosylated N-acylated 4-amino-4,6-dideoxy-α-D-mannopyranosyl residues in the O-chains of their respective lipopolysaccharides.

Molecular characterization of Vibrio anguillarum biotype 2

1981 ◽  
Vol 27 (10) ◽  
pp. 1011-1018 ◽  
Author(s):  
Michael H. Schiewe ◽  
Jorge H. Crosa
Keyword(s):  
Vibrio Anguillarum ◽  
Virulence Plasmid ◽  
Multicopy Plasmid ◽  
Nick Translation ◽  
Fish Pathogens ◽  
Mole Percent ◽  
Discrete Nature ◽  
Sequence Relationships ◽  
Restriction Endonuclease Cleavage

Examination of polynucleotide sequence relationships among 11 strains of Vibrio anguillarum biotype 2 isolated from moribund fish in North America and Japan demonstrated the highly conserved character of this group of fish pathogens and, moreover, confirmed its discrete nature from V. anguillarum biotype 1. Additional molecular analyses of the V. anguillarum biotype 2 strains revealed the universal presence of a multicopy plasmid with a molecular mass of approximately 20 × 106 daltons and a mole percent guanine plus cytosine of 44. The plasmid of strain DF3K was representative of this molecular species and was designated pMJ101. Subsequent DNA–DNA hybridizations using nick translation-labeled pMJ101 as a probe indicated all the 20 × 106 dalton plasmids were either identical or highly conserved, and, furthermore, that pMJ101 was apparently unrelated to either the virulence plasmid, pJM1, of V. anguillarum biotype 1 or to representatives of common plasmid incompatibility groups. The lack of relatedness between pMJ101 and pJM1 was further supported by differences in their restriction endonuclease cleavage patterns.

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