Abstract
Microorganisms can produce many antibiotics against bacteria and fungi, which have been used as potential choice of new antibiotics. In this paper, Bacillus cereus, was used to study the properties of antibacterial substance in its fermentation supernatant. The results showed that the source of antibacterial activity of Bacillus cereus was mainly the antibacterial substance produced by acid precipitation method, and further characterization speculates that the antibacterial substance may be lipopeptide substance. Then the antibacterial spectrum of the antibacterial substance was investigated, which showed that the antibacterial substance only had good inhibitory effect on Gram-positive bacteria and fungi, and selenium enrichment could significantly enhance the antibacterial activity of lipopeptide antibacterial substance produced by Bacillus cereus, and the inhibition mode of antibacterial substance to indicator bacteria was determined. The effects of different treatment methods on the stability of antibacterial substance were studied and the results showed that the antibacterial substance were stable to heat, ultrasonic and ultraviolet treatment, and their antibacterial activity would not be greatly affected. However, they were sensitive to pepsin. The optimum pH range of antibacterial activity was 3-5. This study may contribute to reuse the fermentation supernatant often discarded in the previous fermentation process. At the same time, the lipopeptide antibacterial substance extracted from the fermentation broth of selenium enriched Bacillus cereus can be used in the development of antibiotics and biopesticides, and open up a new way for the control of plant diseases.