Biodegradation of soil humic acids by Streptomyces viridosporus

1992 ◽  
Vol 38 (3) ◽  
pp. 203-208 ◽  
Author(s):  
C. Yanze Kontchou ◽  
Roland Blondeau
Keyword(s):  
Humic Acid ◽  
Humic Acids ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sole Source ◽  
White Rot ◽  
White Rot Fungus ◽  
Mineral Salts ◽  
Experimental Conditions ◽  
Streptomyces Viridosporus ◽  
Soil Humic Acids

Biodegradation of soil humic acids by Streptomyces viridosporus ATCC 39115 growing in a mineral salts – glucose medium was demonstrated. This biodegradation accompanies bacterial growth and is, therefore, presumed to be a primary metabolic activity, but humic acids were not used as the sole source of carbon. This bacterial activity was enhanced when cells were shaken and within a pH range of 6.5–8.5. In further experiments, the relative abilities of S. viridosporus to mineralize [14C]melanoidin, used as synthetic humic acid, were also established. In contrast to the white rot fungus Phanerochaete chrysosporium, another microorganism exhibiting humic acid degrading activity at acidic pH, poor extracellular activities were found in culture medium of S. viridosporus, and veratryl alcohol does not result in increased humic acid degradation. In spite of some peroxidase activity measured in culture filtrates and analyzed by polyacrylamide gel electrophoresis, the humic acid degrading system of S. viridosporus, in these experimental conditions, seems to be cell associated. Key words: humic acid biodegradation, melanoidin mineralization, Streptomyces viridosporus, cell-bound humic acids.

Catabolic activity on humic acids of Streptomyces viridosporus grown under oxygen

1993 ◽  
Vol 39 (10) ◽  
pp. 987-989 ◽  
Author(s):  
C. Yanze Kontchou ◽  
Max Béchet ◽  
Roland Blondeau
Keyword(s):  
Humic Acid ◽  
Batch Culture ◽  
Growth Medium ◽  
Humic Acids ◽  
Aerial Mycelium ◽  
Glucose Medium ◽  
Mineral Salts ◽  
Catabolic Activity ◽  
Streptomyces Viridosporus

When Streptomyces viridosporus was grown in batch culture on mineral salts – glucose medium and under oxygen, aerial mycelium formation and sporulation were suppressed. Under these conditions, biodegradation of soil or synthetic humic acids (expressed as the rate of decolorization of growth medium and the loss of radioactivity of labeled melanoidin, respectively) increased. Concurrently, both the catabolic activity of cells (expressed as peroxidase production, measured by the oxidation of N,N,N′,N′-tetramethyl-1,4-phenylenediamine as substrate) and the excretion of siderophores were stimulated.Key words: humic acid biodegradation, melanoidin mineralization, Streptomyces viridosporus, peroxidase, siderophore.

scholarly journals Relation of chemical properties of soil humic acids to decolorization by white rot fungus–Coriolus consors

2003 ◽  
Vol 49 (2) ◽  
pp. 201-206 ◽  
Author(s):  
Yukiko Yanagi ◽  
Seiji Hamaguchi ◽  
Hajime Tamaki ◽  
Takeshi Suzuki ◽  
Hiroo Otsuka ◽  
...  
Keyword(s):  
Humic Acids ◽  
Chemical Properties ◽  
White Rot ◽  
White Rot Fungus ◽  
Soil Humic Acids ◽  
Properties Of Soil

The Chemistry of Fungal Humic Acid-like Polymers and of Soil Humic Acids

1973 ◽  
Vol 37 (2) ◽  
pp. 229-236 ◽  
Author(s):  
M. Schnitzer ◽  
M. I. Ortiz de Serra ◽  
K. Ivarson
Keyword(s):  
Humic Acid ◽  
Humic Acids ◽  
Soil Humic Acids

Isolation of a new laccase isoform from the white-rot fungiPycnoporus cinnabarinusstrain ss3

2000 ◽  
Vol 46 (8) ◽  
pp. 759-763 ◽  
Author(s):  
Ludovic Otterbein ◽  
Eric Record ◽  
David Chereau ◽  
Isabelle Herpoël ◽  
Marcel Asther ◽  
...  
Keyword(s):  
First Generation ◽  
Polyacrylamide Gel Electrophoresis ◽  
Anion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Amino Acid Sequences ◽  
White Rot ◽  
White Rot Fungus ◽  
Pycnoporus Cinnabarinus ◽  
Ammonium Sulphate Precipitation ◽  
Liquid Culture Medium

Two extracellular laccase isoforms (Lac I and Lac II) produced by the white-rot fungus Pycnoporus cinnabarinus from the monokaryotic strain ss3 were purified from ferulic-acid-induced liquid culture medium using ammonium sulphate precipitation, followed by anion-exchange chromatography on a Mono Q column. Strain ss3 is the first generation of the parental strain P. cinnabarinus I-937. The new isolated isoform, Lac II, consists of an 86 000 molecular weight protein as determined by SDS polyacrylamide gel electrophoresis. The N-terminal amino acid sequences of both isoforms were determined, and compared to known laccase protein sequences of other organisms.Key words: oxydo-reductase, filamentous fungi, purification.

Effect of humic acid, sodium, and calcium additions on the formation of water-stable aggregates in Western Australian wheatbelt soils

Soil Research ◽  
2004 ◽  
Vol 42 (4) ◽  
pp. 435 ◽  
Author(s):  
T. Yamaguchi ◽  
T. Takei ◽  
Y. Yazawa ◽  
M. T. F. Wong ◽  
R. J. Gilkes ◽  
...  
Keyword(s):  
Humic Acid ◽  
Humic Acids ◽  
Coal Waste ◽  
Experimental Conditions ◽  
Water Stable Aggregates ◽  
Acid Effect ◽  
Anionic Groups ◽  
Positive Effect ◽  
Western Australian ◽  
Selection Of

The effect of humic acids extracted from coal waste and peat on the formation of water-stable aggregates in kaolinitic soils from the wheatbelt of Western Australia and a prepared kaolinite–quartz mix were assessed. This effect was compared with that produced by adding calcium to the soils. In the case of kaolinite–quartz mix, the treated samples were measured for micro-aggregation since macro-aggregates were not formed under the experimental conditions. The wheatbelt soils were already aggregated and the measurement was of the further macro-aggregation of these aggregates. In kaolinite–quartz, calcium additions alone enhanced the formation of stable micro-aggregates. This effect was amplified with additions of humic acids when up to 30% of the clay-sized particles were aggregated into larger aggregates. This humic acid effect was not observed in the sodium dominated mineral system.In contrast to the kaolinite-quartz samples, the selection of wheatbelt soils used in this study responded little to calcium additions alone. Humic acid increased the size of aggregates in the sodium-treated soils presumably due to the presence of aluminium in the system studied at pH 4.5. The aluminium ions can be expected to act as the bridging cations between the negatively charged clay-sized particles and the anionic groups present in the organic matter. The ineffectiveness of calcium in these soils allowed the positive effect of the humic acids to be expressed clearly. These findings provide another management option for improving the soil structure of salt-affected land because some of these soils are unlikely to respond to gypsum application.

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