Competition for nodule occupancy of introduced Bradyrhizobium japonicum strain SMGS1 in French soils already containing Bradyrhizobium japonicum strain G49

1993 ◽  
Vol 39 (11) ◽  
pp. 1022-1028 ◽  
Author(s):  
X. Pinochet ◽  
F. Arnaud ◽  
J. C. Cleyet-Marel
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Field Experiments ◽  
Physiological State ◽  
Viable Cell ◽  
Field Trials ◽  
Nodule Occupancy ◽  
Glycine Max L ◽  
Interstrain Competition ◽  
Inoculation Technology ◽  
Japonicum Strain

The competitiveness of Bradyrhizobium japonicum strains G49 and SMGS1 was first studied in the greenhouse in sterilized sand, with or without added soil. Strain SMGS1 was more competitive than strain G49 with soybean (Glycine max L.) cultivar Labrador but the two strains showed equivalent competitiveness with cultivar Kingsoy. When soil was added, nodule occupancy of strain G49 was only 22% with this cultivar. In field experiments, conducted over 2 years in soils already containing strain G49 (1.5 × 103 to 4.0 × 104 cells/g of soil), nodule occupancy of inoculated strain SMGS1 ranged from 20 to 90%. Nodule occupancy was 3–22% higher when inoculation was done by peat seed coating or with liquid inoculation in the row than with peat-coated clay microgranulars. Nodule occupancy was also dependent on the physiological state of the inoculated cells. When an inoculum stored at 28 °C for 1 year was used at the same viable cell rate, nodule occupancy of strain SMGS1 was 4–20% lower than with a recently made inoculum. Pot experiments with soil from field experiments carried out in the 1st year showed that the inoculated strain continued forming nodules without further inoculation, with a recovery rate equivalent to that of field experiment in the previous year.Key words: Bradyrhizobium japonicum, interstrain competition, inoculation technology, ELISA, field trials.

Persistence of introduced Bradyrhizobium japonicum strains in forming nodules in subsequent years after inoculation in Wisconsin soils

1990 ◽  
Vol 36 (11) ◽  
pp. 794-800 ◽  
Author(s):  
T. J. McLoughlin ◽  
S. G. Alt ◽  
P. A. Merlo
Keyword(s):  
Key Words ◽  
Bradyrhizobium Japonicum ◽  
Field Experiments ◽  
Field Trials ◽  
Phage Typing ◽  
Antibiotic Resistant ◽  
Almost All ◽  
Successful Inoculation ◽  
Japonicum Strain ◽  
Different Soils

Nodulation of soybeans by indigenous and inoculum strains of Bradyrhizobium japonicum was studied in field experiments in Wisconsin from 1983 to 86. Aqueous suspensions of bacteria were applied to seeds at the time of planting at levels of 7 × 107–1010 bacteria per 2.5-cm row. The predominant indigenous serogroup was 123 in these soils. Six different inoculum strains were used (two from serocluster 123, two from serogroup 110, and one each from serogroups 122 and C1). Nodule occupants were identified using spontaneous antibiotic-resistant mutations in the inoculum strains, phage typing, and serotyping. In the 1983 experiment, the majority of nodules were formed by the inoculum strains in almost all cases (up to 100% in some cases), in two different soils containing 3.5 × 105 indigenous B. japonicum per gram. After 2 years without inoculation at the same two site, the inoculum strains did not form many nodules on uninoculated soybeans (less than 10% in most cases; less than 30% in all cases). In inoculation experiments carried out in 1985 and 1986, four inoculum strains were used (3 members of 123 serocluster and USDA 110str); inocula containing 108 bacteria per 2.5-cm row formed less than42%ofthe nodules in soils containing 1 × 104–4 × 104B. japonicum per gram. The major conclusions are (i) the success of inoculation in Midwestern U.S. soils is highly variable, even with members of the (highly competitive) 123 serocluster, and (ii) successful inoculation in 1 year in a Wisconsin soil does not ensure that the inoculated strain will persist in forming nodules in that field in subsequent years without further inoculation. Key words: Bradyrhizobium japonicum, strain persistence, field trials.

scholarly journals Polyvinylpyrrolidone (PVP) and Na-Alginate Addition Enhances the Survival and Agronomic Performances of a Liquid Inoculant of Bradyrhizobium japonicum for Soybean (Glycine max (L.) Merr.)

Agronomy ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1009
Author(s):  
Pulak Maitra ◽  
Jubair Al-Rashid ◽  
Dipa Mandal ◽  
Md. Shofiul Azam ◽  
Noorain Munim Rasul
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Field Experiments ◽  
Nodule Number ◽  
Organic Cultivation ◽  
Cell Counts ◽  
Rdna Sequencing ◽  
Glycine Max L ◽  
Number Of Seeds ◽  
Japonicum Strain ◽  
Liquid Inoculant

Nontoxic polymers PVP and Na-alginate may provide a favorable environment for the survival of bacteria. Therefore, PVP and Na-alginate were added to a growth medium to develop a liquid inoculant of Bradyrhizobium japonicum strain. The strain was identified by 16S rDNA sequencing. The addition of PVP (1.8%) and Na-alginate (0.2%) in the medium promoted a better survival (1.93 × 109 cells mL−1) of B. japonicum strain compared to the control (3.50 × 102 cells mL−1) after 6 months of storage. The combination of PVP and Na-alginate ensured 1.53 × 107 cells mL−1 up to 12 months of storage under ambient temperature (28 ± 2 °C), whereas PVP (1.8%) or Na-alginate (0.2%) alone produced similar cell counts only up to 8 months and 6 months, respectively. Consecutive field experiments proved the efficacy of the liquid inoculant on nodulation and yield of soybean. The combination of PVP and Na-alginate-based inoculation of B. japonicum strain significantly increased the nodule number per plant, number of pods per plant, number of seeds per pod, seed yield, and yield per hectare (p ≤ 0.05). Thus, the combination of PVP- and Na-alginate-based inoculation of B. japonicum has great potential to popularize the organic cultivation of soybean.

Competition for nodule occupancy of introduced Bradyrhizobium japonicum strains in a Wisconsin soil with a low indigenous bradyrhizobia population

1990 ◽  
Vol 36 (12) ◽  
pp. 839-845 ◽  
Author(s):  
T. J. McLoughlin ◽  
S. Hearn ◽  
S. G. Alt
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Growing Season ◽  
Field Trials ◽  
Enzyme Linked Immunosorbent Assay ◽  
Nodule Occupancy ◽  
Antibiotic Resistant ◽  
Growing Seasons ◽  
Low Incidence ◽  
One Year ◽  
Successful Inoculation

The population dynamics of six introduced Bradyrhizobium japonicum strains were measured over three growing seasons in a Wisconsin soil with a low incidence of indigenous B. japonicum (10 cells/gm). Four antibiotic-resistant members of the 123 serocluster (which were either spectinomycin resistant or streptomycin resistant), USDA 110, and USDA 138 were inoculated using liquid inoculum, at a rate of 1 × 108 cells per 2.5-cm row, on two soybean cultivars in 1985. Nodule occupants were identified using an enzyme-linked immunosorbent assay (ELISA), fluorescent antibodies, and antibiotic-resistant mutants. In the first growing season, 100% of the nodules were formed by the introduced strains. The nodules from the uninoculated plots were occupied by an indigenous 110 serogroup. In the second and the third season at the same site (without further inoculation), a high percentage (> 60%) of the nodules from all the plots were nodulated by the 123 serocluster (either alone or as mixed infections). However, < 25% of the nodules in the 123-inoculated plots and < 9% in the other plots were formed by any of the antibiotic-marked 123 inoculum strains introduced in 1985. The main conclusions are (i) that it is possible to successfully introduce inoculum strains in soils where the indigenous Bradyrhizobium population is low and to obtain 100% nodule occupancy in the first growing season, and (ii) that successful inoculation in one year in soils with a low incidence of Bradyrhizobium does not ensure that the introduced inoculum strains will form nodules in subsequent years. Key words: Bradyrhizobium japonicum, indigenous bradyrhizobia, interstrain competition, field trials.

Intra- and inter-specific competition in Rhizobium fredii and Bradyrhizobium japonicum as indigenous and introduced organisms

1987 ◽  
Vol 33 (11) ◽  
pp. 990-995 ◽  
Author(s):  
Stephen F. Dowdle ◽  
B. Ben Bohlool
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Nodule Occupancy ◽  
Soybean Field ◽  
Republic Of China ◽  
Rhizobium Inoculation ◽  
Rhizobium Fredii ◽  
History Of ◽  
Competitive Success ◽  
Glycine Max L ◽  
Different Soils

We studied the competition between Bradyrhizobium japonicum and Rhizobium fredii isolates for nodulation of soybean (Glycine max L. Merrill) cultivars Williams and Ai Jiao Zao grown in three different soils in pots. Two of the soils were from People's Republic of China, one from a soybean field in Honghu with no history of Rhizobium inoculation, and one from a rice field in Wuhan with no history of soybean cultivation. The Honghu soil contained B. japonicum and R. fredii (log total number g−1 = 5.82 ± 0.58); whereas the Wuhan soil only contained B. japonicum (log total number g−1 = 2.80 ± 0.52). Inoculation did not result in a significant increase in nodule number on plants in either soil. Uninoculated plants of both cultivars harbored only R. fredii in the Honghu soil and only B. japonicum in the Wuhan soil. Even when B. japonicum were inoculated into the Honghu soil, R. fredii occupied the majority of the nodules on both cultivars. In the Wuhan soil, B. japonicum serogroups USDA110 and USDA136b (= CB1809) occupied the majority of the nodules except when an isolate of R. fredii from the soybean soil was added in high numbers. In a Hawaiian soil devoid of B. japanicum or R. fredii, when soybeans were inoculated with isolates of both species, most of the nodules were formed by B. japonicum. The R. fredii isolate could form up to 20% of nodules in this soil, but only on the Ai Jia Zao cultivar. In the Wuhan but not the Hawaiian soil, peat pelleting of seeds with equal numbers of two B. japonicum and one R. fredii isolates increased nodule occupancy by B. japonicum USDA136b serogroup significantly as compared with when the same isolates were inoculated into the soil. The results reported here highlight the critical importance of being indigenous to the competitive success of B. japonicum and R. fredii in nodulation of their soybean host.

Effect of numbers of Bradyrhizobium japonicum applied in commercial inoculants on soybean seed yield in Ontario

1992 ◽  
Vol 38 (6) ◽  
pp. 588-593 ◽  
Author(s):  
D. J. Hume ◽  
D. H. Blair
Keyword(s):  
Glycine Max ◽  
Seed Yield ◽  
Bradyrhizobium Japonicum ◽  
Field Experiments ◽  
Soybean Seed ◽  
Yield Response ◽  
Cubic Equation ◽  
Glycine Max L ◽  
Seed Yields ◽  
Commercial Inoculants

In the absence of Bradyrhizobium japonicum populations in the soil, yields of field-grown soybean (Glycine max (L.) Merrill) usually respond to inoculation with B. japonicum. The objective of this research was to determine the relationship between numbers of B. japonicum per seed in inoculants and soybean nodulation and yield. A total of six field experiments were conducted in 1989 and 1990 on new soybean soils. In dilution trials, Grip inoculant was applied to provide approximately 106, 105, 104, and 103B. japonicum per seed at two locations in 1989. Nodule number and mass, as well as seed yield, increased curvilinearly upward with increasing log10 most probable numbers (MPNs) of B. japonicum. The yield response curve was best fit by a cubic equation, which accounted for 97% of the variation in yield. Seed yields increased 19% (1.83 to 2.13 Mg/ha) from 105 to 106B. japonicum per seed. In field experiments involving 8 commercial inoculants in 1989 and 10 in 1990, and conducted at two locations in each year, responses to increasing log MPNs in the inoculants also were concave upwards and cubic. In the two years, 78 and 46% of the yield variation was accounted for by log MPN per seed. Increasing MPN per seed from 105 to 106 improved yields in first-time fields by an average of 24%, indicating the present minimum standard of 105B. japonicum per seed should be increased. Key words: most probable numbers, response to inoculation, nodulation, Glycine max (L.) Merrill.

scholarly journals Identification and use of actinomycetes for enhanced nodulation of soybean co-inoculated with Bradyrhizobium japonicum

2003 ◽  
Vol 49 (8) ◽  
pp. 483-491 ◽  
Author(s):  
A K Gregor ◽  
B Klubek ◽  
E C Varsa
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Antibiotic Sensitivity ◽  
Nodule Occupancy ◽  
Antibiotic Resistant ◽  
Glycine Max L ◽  
Resistant Strains ◽  
Soybean Plants ◽  
Population Sensitivity ◽  
Germinated Soybean ◽  
Streptomyces Kanamyceticus

The utilization of actinomycetes as potential soybean (Glycine max (L.)) co-inoculants was evaluated. Soil samples from Carbondale and Belleville, Ill., were used to inoculate pre-germinated soybean plants to determine antibiotic sensitivity in the native Bradyrhizobium japonicum population. Sensitivity was in the order kanamycin > tetracycline > oxytetracycline > rifampicin > neomycin. Antagonism by five actinomycete cultures toward seven test strains of B. japonicum was also assessed. The ranking average inhibition (across all seven B. japonicum strains) by these actino mycetes was Streptomyces kanamyceticus = Streptomyces coeruleoprunus > Streptomyces rimosus > Streptomyces sp. > Amy colatopsis mediterranei. Ten antibiotic combinations were used to isolate antibiotic-resistant mutants of B. japonicum I-110 and 3I1B-110 via successive cycles of mutation. Eighty-one antibiotic-resistant strains were isolated and tested for symbiotic competency; nine of which were selected for further characterization in a greenhouse pot study. Few differences in nodule number were caused by these treatments. Nodule occupancy varied from 0% to 18.3% when antibiotic-resistant strains of B. japonicum were used as the sole inoculants. However, when three mutant strains of B. japonicum were co-inoculated with S. kanamyceticus, significant increases in nodule occupancy (up to 55%) occurred. Increases in shoot nitrogen composition (27.1%–40.9%) were also caused by co-inoculation with S. kanamyceticus. Key words: Bradyrhizobium japonicum, Streptomyces kanamyceticus, indigenous bradyrhizobia, co-inoculation, nodule occupancy.

scholarly journals Efficacy of Peat and Liquid Inoculant Formulations of Bradyrhizobium japonicum Strain WB74 on Growth, Yield and Nitrogen Concentration of Soybean (Glycine max L.)

Nitrogen ◽  
2021 ◽  
Vol 2 (3) ◽  
pp. 332-346
Author(s):  
Auges Gatabazi ◽  
Barend Juan Vorster ◽  
Mireille Asanzi Mvondo-She ◽  
Edgar Mangwende ◽  
Robert Mangani ◽  
...  
Keyword(s):  
South Africa ◽  
Glycine Max ◽  
Bradyrhizobium Japonicum ◽  
Sandy Loam ◽  
Nitrogen Concentration ◽  
Growth And Yield ◽  
Bioclimatic Zone ◽  
Glycine Max L ◽  
Loam Soil ◽  
Japonicum Strain

South African soils generally lack native Bradyrhizobium strains that nodulate and fix atmospheric nitrogen (N2) in soybeans (Glycine max L.). It is therefore very important to inoculate soybeans with products that contain effective Bradyrhizobium strains as active ingredients. In this study, a field experiment was conducted on two bioclimatic zones in South Africa during the 2019/2020 season to assess the effect of Bradyrhizobium japonicum strain WB74 inoculant formulation on nitrogen fixation, growth and yield improvement in soybeans. The first bioclimatic zone was characterized by a sandy clay loam soil, whereas the second bioclimatic zone has a sandy loam soil. The results showed that inoculation of soybeans with both peat and liquid formulations of Bradyrhizobium japonicum WB74 increased nitrogen uptake, which resulted in yield increase. The amount of N fixed was measured as 15N isotopes and increased with all treatments compared to the uninoculated control in both liquid and peat inoculant formulations. In bioclimatic zone A, slightly better results were obtained using the liquid formulation (1.79 t ha−1 for liquid compared to 1.75 t ha−1 for peat treatments), while peat formulations performed better in bioclimatic zone B (1.75 t ha−1 for peat compared to 1.71 t ha−1 for liquid treatments). In both areas higher yields were obtained with the formulations used in this study compared to the registered standards (treatment T3). The findings in this study provide vital information in the development and application of formulated microbial inoculants for sustainable agriculture in South Africa.

Phage coating of soybean seed reduces nodulation by indigenous soil bradyrhizobia

1992 ◽  
Vol 38 (12) ◽  
pp. 1264-1269 ◽  
Author(s):  
H. Abdel Basit ◽  
J. Scott Angle ◽  
S. Salem ◽  
E. M. Gewaily
Keyword(s):  
Glycine Max ◽  
Bradyrhizobium Japonicum ◽  
Nitrogenase Activity ◽  
Soybean Seed ◽  
Nodule Occupancy ◽  
Field Soil ◽  
Control Value ◽  
Glycine Max L ◽  
Inoculum Strain ◽  
Plant Basis

Inoculation of soybean with Bradyrhizobium japonicum is often unsuccessful owing to the failure of inoculum strains to nodulate soybeans (Glycine max (L.) Merr.) in the presence of indigenous strains of rhizobia in soil. Previous studies have shown that it is possible to reduce nodulation with indigenous strains of rhizobia by amending the soil with a bacteriophage specific for the indigenous strain. The objective of the current study was to determine whether the coating of seed with phage affected nodule occupancy and soybean growth. A phage specific for B. japonicum USDA 469 and a symbiotically superior strain of rhizobium (B. japonicum USDA 110) were coated together onto soybean seed and planted into both greenhouse and field soil previously inoculated with B. japonicum USDA 469. The phage coated onto seed reduced nodulation by B. japonicum USDA 469 to 48% occupancy, compared with 64% for the untreated control value. Nodulation by the superior inoculum strain was increased from 48 to 82% occupancy by coating seed with the homologous phage and B. japonicum USDA 110. The rate of nitrogenase activity (on a per plant basis) was increased by coating seed with the phage and B. japonicum USDA 110. No other plant or symbiotic parameters were affected by phage coating of seed. These results indicate that the nodulation of soybeans can be significantly affected by the coating of seed with phage specific for undesirable strains of rhizobia in soil and the concurrent coating of seed with desirable strains of rhizobia. Key words: competition, rhizobiophage, rhizobia, soybeans.

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