Phage coating of soybean seed reduces nodulation by indigenous soil bradyrhizobia

1992 ◽  
Vol 38 (12) ◽  
pp. 1264-1269 ◽  
Author(s):  
H. Abdel Basit ◽  
J. Scott Angle ◽  
S. Salem ◽  
E. M. Gewaily
Keyword(s):  
Glycine Max ◽  
Bradyrhizobium Japonicum ◽  
Nitrogenase Activity ◽  
Soybean Seed ◽  
Nodule Occupancy ◽  
Field Soil ◽  
Control Value ◽  
Glycine Max L ◽  
Inoculum Strain ◽  
Plant Basis

Inoculation of soybean with Bradyrhizobium japonicum is often unsuccessful owing to the failure of inoculum strains to nodulate soybeans (Glycine max (L.) Merr.) in the presence of indigenous strains of rhizobia in soil. Previous studies have shown that it is possible to reduce nodulation with indigenous strains of rhizobia by amending the soil with a bacteriophage specific for the indigenous strain. The objective of the current study was to determine whether the coating of seed with phage affected nodule occupancy and soybean growth. A phage specific for B. japonicum USDA 469 and a symbiotically superior strain of rhizobium (B. japonicum USDA 110) were coated together onto soybean seed and planted into both greenhouse and field soil previously inoculated with B. japonicum USDA 469. The phage coated onto seed reduced nodulation by B. japonicum USDA 469 to 48% occupancy, compared with 64% for the untreated control value. Nodulation by the superior inoculum strain was increased from 48 to 82% occupancy by coating seed with the homologous phage and B. japonicum USDA 110. The rate of nitrogenase activity (on a per plant basis) was increased by coating seed with the phage and B. japonicum USDA 110. No other plant or symbiotic parameters were affected by phage coating of seed. These results indicate that the nodulation of soybeans can be significantly affected by the coating of seed with phage specific for undesirable strains of rhizobia in soil and the concurrent coating of seed with desirable strains of rhizobia. Key words: competition, rhizobiophage, rhizobia, soybeans.

scholarly journals Soybean Seed Extracts Preferentially Express Genomic Loci of Bradyrhizobium japonicum in the Initial Interaction with Soybean, Glycine max (L.) Merr

DNA Research ◽  
2008 ◽  
Vol 15 (4) ◽  
pp. 201-214 ◽  
Author(s):  
M. Wei ◽  
T. Yokoyama ◽  
K. Minamisawa ◽  
H. Mitsui ◽  
M. Itakura ◽  
...  
Keyword(s):  
Glycine Max ◽  
Bradyrhizobium Japonicum ◽  
Soybean Seed ◽  
Glycine Max L ◽  
Initial Interaction ◽  
Seed Extracts

Effect of numbers of Bradyrhizobium japonicum applied in commercial inoculants on soybean seed yield in Ontario

1992 ◽  
Vol 38 (6) ◽  
pp. 588-593 ◽  
Author(s):  
D. J. Hume ◽  
D. H. Blair
Keyword(s):  
Glycine Max ◽  
Seed Yield ◽  
Bradyrhizobium Japonicum ◽  
Field Experiments ◽  
Soybean Seed ◽  
Yield Response ◽  
Cubic Equation ◽  
Glycine Max L ◽  
Seed Yields ◽  
Commercial Inoculants

In the absence of Bradyrhizobium japonicum populations in the soil, yields of field-grown soybean (Glycine max (L.) Merrill) usually respond to inoculation with B. japonicum. The objective of this research was to determine the relationship between numbers of B. japonicum per seed in inoculants and soybean nodulation and yield. A total of six field experiments were conducted in 1989 and 1990 on new soybean soils. In dilution trials, Grip inoculant was applied to provide approximately 106, 105, 104, and 103B. japonicum per seed at two locations in 1989. Nodule number and mass, as well as seed yield, increased curvilinearly upward with increasing log10 most probable numbers (MPNs) of B. japonicum. The yield response curve was best fit by a cubic equation, which accounted for 97% of the variation in yield. Seed yields increased 19% (1.83 to 2.13 Mg/ha) from 105 to 106B. japonicum per seed. In field experiments involving 8 commercial inoculants in 1989 and 10 in 1990, and conducted at two locations in each year, responses to increasing log MPNs in the inoculants also were concave upwards and cubic. In the two years, 78 and 46% of the yield variation was accounted for by log MPN per seed. Increasing MPN per seed from 105 to 106 improved yields in first-time fields by an average of 24%, indicating the present minimum standard of 105B. japonicum per seed should be increased. Key words: most probable numbers, response to inoculation, nodulation, Glycine max (L.) Merrill.

scholarly journals Effect of soybean (Glycine max (L.) Merill) treatments on the introduction capacity of endophytic bacteria

2020 ◽  
Author(s):  
I.N. Ananyeva ◽  
◽  
Z.M. Aleschenkova ◽  
P.V. Rybaltovskaya ◽  
M.A. Chindareva ◽  
...  
Keyword(s):  
Glycine Max ◽  
Plant Height ◽  
Bradyrhizobium Japonicum ◽  
Endophytic Bacteria ◽  
Seed Treatment ◽  
Control Plant ◽  
Soybean Seed ◽  
Nitrogen Fixing ◽  
Antibiotic Resistant ◽  
Glycine Max L

The goal of the work was to obtain antibiotic-resistant forms of endophytic Glycine max L. (Merill) bacteria and to study their introduction potential affected by different seed treatment methods. Rifampicin-resistant variants of endophytic soybean bacteria Rhizobium radiobacter 27c and Pseudomonas fluorescens 11E preserving valuable properties were derived. Soybean seed treatment with Bradyrhizobium japonicum BIM V-501D and endophytic nitrogen-fixing Rh. radiobacter 27c, phosphate-mobilizing Ps. fluorescens 11E bacteria under model conditions promoted accumulation of nitrogen-fixing bacteria in the root, stem and leaves. The number of nodules rose by 70% compared with the mono-inoculated control; plant height increased by 19%.

Sampling to determine nodule occupancy of soybean (Glycine max (L) Merr.) by Bradyrhizobium japonicum strains

1998 ◽  
Vol 44 (8) ◽  
pp. 795-799 ◽  
Author(s):  
Cécile Revellin ◽  
Jean-Baptiste Denis ◽  
Gérard Catroux
Keyword(s):  
Glycine Max ◽  
Confidence Interval ◽  
Bradyrhizobium Japonicum ◽  
Sampling Strategy ◽  
Nodule Occupancy ◽  
Experimental Conditions ◽  
Anova Model ◽  
Glycine Max L ◽  
Approximate Confidence Intervals ◽  
Time Required

The confidence interval of the percentage of soybean (Glycine max. (L.) Merr.) nodule occupancy by two strains of Bradyrhizobium japonicum was studied using samples of nodules per plant varying by multiples of 8 (8-56). These samples were recovered from all of the nodules of four sets of 21-24 plants grown in soil colonized only by these strains and typed using strain-specific sera. Approximate confidence intervals (ACI) were estimated (from the ANOVA model) using the formula, length of ACI = 4 [(0.040/P) + (0.278/PN)]1/2, where P is the number of sampled plants and N is the number of nodules characterised for each plant. The estimated ACI decreased when the number of plants sampled increased and decreased to a lesser extent when the number of nodules typed per plant increased. It was possible to define a plant and nodule sampling strategy to minimize the labor and time required to get acceptable ACI values. Under our experimental conditions, when 192 nodules were typed, combinations of 24 plants × 8 nodules per plant and 32 plants × 6 nodules per plant were the most satisfactory, corresponding to calculated ACI of 22.3 and 20.8%, respectively.Key words: Bradyrhizobium japonicum, competition, method, nodule occupancy, soybean.

Subchronic Toxicity of Lunasin Targeted Extract (ET-Lun) from Soybean Seed (Glycine max (L.) Merr.): Perspective from Liver Histopathology, SGOT, and SGPT Levels in Sprague Dawley Rats

2021 ◽  
Vol 13 (6) ◽  
pp. 1384-1388
Author(s):  
Numlil Khaira Rusdi ◽  
Weri Lia Yuliana ◽  
Erni Hernawati Purwaningsih ◽  
Andon Hestiantoro ◽  
Kusmardi Kusmardi
Keyword(s):  
Glycine Max ◽  
Soybean Seed ◽  
Sprague Dawley ◽  
Subchronic Toxicity ◽  
Liver Histopathology ◽  
Sprague Dawley Rats ◽  
Glycine Max L

scholarly journals Nodulation efficacy of Bradyrhizobium japonicum inoculant strain WB74 on soybean (Glycine max L. Merrill) is affected by several limiting factors

2014 ◽  
Vol 8 (20) ◽  
pp. 2069-2076 ◽  
Author(s):  
Idris Hassen Ahmed ◽  
L. Bopape Francina ◽  
H. Rong Isabella ◽  
Seane Galaletsang
Keyword(s):  
Glycine Max ◽  
Bradyrhizobium Japonicum ◽  
Limiting Factors ◽  
Inoculant Strain ◽  
Glycine Max L

scholarly journals PERUBAHAN BENTUK DAN UKURAN BENIH DUA KULTIVAR KEDELAI (Glycine max (L.) Merr.) DI JATINANGOR DAN CIKAJANG

2017 ◽  
Vol 5 (1) ◽  
pp. 1
Author(s):  
Siti Rodiah ◽  
Zulfatunnisa Zulfatunnisa ◽  
Sumadi Sumadi ◽  
Anne Nuraini ◽  
Meddy Rachmadi ◽  
...  
Keyword(s):  
Glycine Max ◽  
Low Temperature ◽  
Seed Size ◽  
Block Design ◽  
Soybean Seed ◽  
Growth Environment ◽  
Randomized Block Design ◽  
Glycine Max L ◽  
Improved Cultivars ◽  
Size Heterogeneity

The variation of the seed size in each species and individuals might be from of difference species adaptation for  a difference environment. This difference may also arise from the constraints of limited formation of seed size. The use of adaptive ciltivars on the growth environment is very influential on the succes in the farm field. This research was aimed to find the adaptation of phase and size seed of two cultivars of soybeans in Jatinangor and Cikajang. This research was held in Jatinangor (Sumedang regency) and Cikajang (Garut regency) from April to July 2016. The design that used in this research was Randomized Block Design (RBD) and Duncan at 5% rate. Improved cultivars that tested in this research were placed at Grobogan and Anjasmoro which were repeated 5 times. The results of experiment showed that adaptation of size seed showed of 100 grains and large seeds. The low temperature condition can increase of variability of seed size. Heterogeneity of environment can not sustain the size of soybean seed. Genetic and environment factors influence significantly for weight of 100 grains and seed size Grobogan in Jatinangor. The weight of 100 grains Grobogan in Jatinangor and Cikajang haved a greater than Anjasmoro. Environmental factors influence yield of soybean, weight of 100 grains of cultivars in Cikajang haved a greater than Jatinangor caused by the seed size.

scholarly journals Diversidad de los rizobios que nodulan la soja en los suelos de la Pampa húmeda e identificación de cepas para la fabricación de inoculantes comerciales

2016 ◽  
Author(s):  
◽  
Graciela N. Pastorino
Keyword(s):  
Glycine Max ◽  
Bradyrhizobium Japonicum ◽  
Glycine Max L

Las labores culturales, provocan modificaciones de las propiedades físicas, químicas y biológicas de los suelos. Estas se clasifican en: labranza convencional, labranza vertical y siembra directa. Cada tecnología de manejo genera estreses a los que las poblaciones microbianas se adaptan, como resultado de cambios morfológicos, fisiológicos y genéticos. La inoculación de la soja (Glycine max [L.] Merr) es una tecnología que se introdujo en la Argentina junto con el cultivo y por ello los inoculantes fueron el vehículo de ingreso de las cepas exóticas de Bradyrhizobium, que una vez incorporadas al suelo, se adaptaron y establecieron dando origen a las poblaciones de rizobios naturalizadas. Lo que condujo a generar diversidad a nivel del genoma de los bradyrizobios. El objetivo de este trabajo fue evaluar la diversidad de los simbiontes de soja que se encuentran en la población del suelo y el rol que cumple como recurso en la selección de cepas para la producción comercial de inoculantes. Se evaluaron dos muestras de suelo que durante los últimos 8 años fueron trabajados con siembra directa (SD) y con labranza convencional (LC). Se realizó el recuento y aislamiento de rizobios. Los aislados se caracterizaron fisiológica y genéticamente. La diversidad se evaluó mediante la amplificación de las secuencias BOX A1R. Se seleccionaron 52 aislados que se identificaron amplificando las regiones RSα y nifD y la secuenciación del fragmento ITS 16S - 23S rDNA. El recuento de rizobios mostró que en el suelo bajo SD y antecesor soja la población fue mayor que en la muestra de suelo bajo LC y antecesor maíz. La caracterización fisiológica de 200 aislados reflejó que los suelos LC contuvieron un mayor número de cepas tolerantes a salinidad y alta temperatura. Además un porcentaje mayor de aislados del suelo LC, respecto del suelo bajo SD, resultó con capacidad de sobrevivencia sobre semilla superior a la cepa control E109. Los aislados además presentaron diferencias en la nodulación y fijación de nitrógeno, en la tolerancia a glifosato y en la producción de ácido indol acético (AIA). Sólo un aislado mostró capacidad de solubilización de fósforo. El análisis del dendrograma generado con los fingerprints BOX A1R, mostró que hay mayor diversidad en los suelos bajo SD. Sobre los 52 aislados analizados en base a la secuencia del ITS se encontró que el 70 % son Bradyrhizobium japonicum y 30 % B. elkanii. Se identificaron 4 aislados que comparten características genéticas de ambas especies de Bradyrhizobium. En esta tesis se confirmó que en los suelos con historia de cultivo de soja, manejados bajo distintos sistemas de labranzas, se indujeron cambios a nivel del genoma de las cepas de rizobios que contribuyó a modificar la diversidad de la población. Las poblaciones naturalizadas son un recurso para la identificación de rizobios con características superiores para la fabricación de inoculantes, sin embargo es necesario profundizar las evaluaciones de la supervivencia, tolerancia, competitividad y promoción del crecimiento vegetal de rizobios naturalizados para emplearlos como bioinoculantes.

scholarly journals EMERGÊNCIA E SOBREVIVÊNCIA DE PLANTAS DE SOJA A CAMPO EM RELAÇÃO ÀS PROFUNDIDADES E ÉPOCAS DE SEMEADURA

2020 ◽  
Vol 35 (2) ◽  
pp. 150-157
Author(s):  
Patrícia Pereira Dias ◽  
Saulo Fernando Gomes de Sousa ◽  
Paulo Roberto Arbex Silva ◽  
Tiago Pereira da Silva Correia ◽  
Anderson Ravanny de Andrade Gomes
Keyword(s):  
Glycine Max ◽  
Statistical Analysis ◽  
Field Experiment ◽  
Analysis Of Variance ◽  
Early Stage ◽  
Soybean Seed ◽  
Sowing Depth ◽  
Tukey Test ◽  
Glycine Max L ◽  
Soil Deposition

A PROFUNDIDADE DE SEMEADURA DA SOJA NA PLANTABILIDADE   PATRÍCIA PEREIRA DIAS1*, SAULO FERNANDO GOMES DE SOUSA2, PAULO ROBERTO ARBEX SILVA3, TIAGO PEREIRA DA SILVA CORREIA4 e ANDERSON RAVANNY DE ANDRADE GOMES5   * Parte do texto foi extraído da tese da autora 1 Departamento de Produção Vegetal, Universidade Estadual Paulista ‘Júlio de Mesquita Filho’ - Av. Universitária, 3780 - Altos do Paraíso, 18610-034, Botucatu - SP, Brasil. [email protected] 2 Agroefetiva, Rua Lourival Ferreira, 11 - Distrito Industrial III, 18608-853, Botucatu - SP, Brasil.  [email protected] 3 Departamento de Engenharia Rural e Socioeconomia, Universidade Estadual Paulista ‘Júlio de Mesquita Filho’ - Av. Universitária, 3780 - Altos do Paraíso, 18610-034, Botucatu - SP, Brasil.  [email protected] 4 Faculdade de Agronomia e Medicina Veterinária, Universidade de Brasília, Caixa - Campos Universitário Darci Ribeiro ICC – Asa Norte, 04508, Brasília - DF, Brasil. [email protected] 5 Faculdade Regional da Bahia, Rodovia AL 220, 3630 - Senador Arnon De Melo - Planalto, Arapiraca – AL, Brasil. [email protected]   RESUMO: O objetivo deste trabalho foi avaliar a cultura da soja semeada a campo em diferentes profundidades das sementes e épocas de semeadura e, dessa maneira, saber o quanto esses fatores influenciam a emergência e sobrevivência das plantas. O experimento foi conduzido em dois anos agrícolas, 2015/16 e 2016/17, com sementes de soja cultivar 5D634, na Fazenda Lageado, da Faculdade de Ciências Agronômicas – UNESP, Botucatu (SP), Brasil. O delineamento foi de blocos ao acaso em esquema fatorial 6 × 2, com seis tratamentos referentes às profundidades das sementes na mesma linha de semeadura: 0,02, 0,05 e 0,08 m do nível do solo, e combinação e alternância entre eles: 0,02 e 0,05, 0,02 e 0,08, 0,05 e 0,08 m e duas épocas de semeadura: outubro e novembro, com quatro repetições, totalizando 48 parcelas. Para a análise estatística, os dados foram submetidos à análise de variância (ANOVA), utilizando o teste de Tukey a 5% de probabilidade. A perda foi acima de 10% no estande de plantas na semeadura mais profunda (0,08 m), aliada à menor precipitação (outubro/2016), que contribuiu significativamente para esse resultado. Desta forma, a plantabilidade é prejudicada com o erro da profundidade de semeadura, aqui representada pela emergência e sobrevivência de plantas.   Palavras-chave: Glycine max L., Plantabilidade, Estande de plantas.   THE SOYBEAN SOWING DEPTH IN PLANTABILITY   ABSTRACT: The aim of this work was evaluate the sowing of soybean seed with error in soil deposition and different month of sowing. The field experiment was carried out during two years (2015 and 2016) at Fazenda Lageado, Faculty of Agronomic Sciences - UNESP / Botucatu - SP, Brazil. The plots were randomized blocks in 6 × 2 factorial, six treatments referring to the depth of the seeds in soil: 0.02 (T1); 0.05 (T2 - control) and 0.08 (T3); 0.02 and 0.05 (T4); 0.02 and 0.08 (T5); 0.05 and 0.08 m (T6) and two sowing times: October and November. For statistical analysis the data were submitted to analysis of variance (ANOVA) using Tukey test at 5% probability. The number of plants in early stage decreased by over 10% in the deep seeding (0.08 m), added to the less rainfall (October / 2016) which contributed significantly to this result. Therefore, plantability is damaged by the error of sowing depth, represented here by the emergence and survival of plants.   Keywords: Glycine max L., Plantability, Early stage.

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