Intra- and inter-specific competition in Rhizobium fredii and Bradyrhizobium japonicum as indigenous and introduced organisms

1987 ◽  
Vol 33 (11) ◽  
pp. 990-995 ◽  
Author(s):  
Stephen F. Dowdle ◽  
B. Ben Bohlool
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Nodule Occupancy ◽  
Soybean Field ◽  
Republic Of China ◽  
Rhizobium Inoculation ◽  
Rhizobium Fredii ◽  
History Of ◽  
Competitive Success ◽  
Glycine Max L ◽  
Different Soils

We studied the competition between Bradyrhizobium japonicum and Rhizobium fredii isolates for nodulation of soybean (Glycine max L. Merrill) cultivars Williams and Ai Jiao Zao grown in three different soils in pots. Two of the soils were from People's Republic of China, one from a soybean field in Honghu with no history of Rhizobium inoculation, and one from a rice field in Wuhan with no history of soybean cultivation. The Honghu soil contained B. japonicum and R. fredii (log total number g−1 = 5.82 ± 0.58); whereas the Wuhan soil only contained B. japonicum (log total number g−1 = 2.80 ± 0.52). Inoculation did not result in a significant increase in nodule number on plants in either soil. Uninoculated plants of both cultivars harbored only R. fredii in the Honghu soil and only B. japonicum in the Wuhan soil. Even when B. japonicum were inoculated into the Honghu soil, R. fredii occupied the majority of the nodules on both cultivars. In the Wuhan soil, B. japonicum serogroups USDA110 and USDA136b (= CB1809) occupied the majority of the nodules except when an isolate of R. fredii from the soybean soil was added in high numbers. In a Hawaiian soil devoid of B. japanicum or R. fredii, when soybeans were inoculated with isolates of both species, most of the nodules were formed by B. japonicum. The R. fredii isolate could form up to 20% of nodules in this soil, but only on the Ai Jia Zao cultivar. In the Wuhan but not the Hawaiian soil, peat pelleting of seeds with equal numbers of two B. japonicum and one R. fredii isolates increased nodule occupancy by B. japonicum USDA136b serogroup significantly as compared with when the same isolates were inoculated into the soil. The results reported here highlight the critical importance of being indigenous to the competitive success of B. japonicum and R. fredii in nodulation of their soybean host.

Competition for nodulation of field-grown soybeans by strains of Rhizobium fredii

1986 ◽  
Vol 32 (2) ◽  
pp. 183-186 ◽  
Author(s):  
Thomas J. McLoughlin ◽  
Scott G. Alt ◽  
P. Ann Owens ◽  
Corrine Fetherston
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Indigenous Population ◽  
Growing Season ◽  
Indigenous Populations ◽  
First Year ◽  
Antibiotic Resistant ◽  
Rhizobium Fredii ◽  
Glycine Max L ◽  
Resistant Mutants ◽  
Antibiotic Resistant Mutants

Nodulation of Glycine max (L) Merr. by six Rhizobium fredii strains was measured in two Midwestern fields containing high indigenous populations of Bradyrhizobium japonicum (3 × 105/gm soil). The soils were inoculated with antibiotic-resistant mutants using liquid inoculum at two levels on soybean cv. Peking and cv. Jacques 130. Strain establishment was measured 40 days after planting. In the first year, USDA206, USDA217, and USDA257 were the most competitive strains, occupying greater than 50% of the nodules on cv. Peking in both soils. None of the strains were competitive on Jacques 130. In the second growing season, all nodules were formed by the indigenous population on both cultivars, suggesting that these fast-growing strains do not persist in Midwestern soils.

scholarly journals Identification and use of actinomycetes for enhanced nodulation of soybean co-inoculated with Bradyrhizobium japonicum

2003 ◽  
Vol 49 (8) ◽  
pp. 483-491 ◽  
Author(s):  
A K Gregor ◽  
B Klubek ◽  
E C Varsa
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Antibiotic Sensitivity ◽  
Nodule Occupancy ◽  
Antibiotic Resistant ◽  
Glycine Max L ◽  
Resistant Strains ◽  
Soybean Plants ◽  
Population Sensitivity ◽  
Germinated Soybean ◽  
Streptomyces Kanamyceticus

The utilization of actinomycetes as potential soybean (Glycine max (L.)) co-inoculants was evaluated. Soil samples from Carbondale and Belleville, Ill., were used to inoculate pre-germinated soybean plants to determine antibiotic sensitivity in the native Bradyrhizobium japonicum population. Sensitivity was in the order kanamycin > tetracycline > oxytetracycline > rifampicin > neomycin. Antagonism by five actinomycete cultures toward seven test strains of B. japonicum was also assessed. The ranking average inhibition (across all seven B. japonicum strains) by these actino mycetes was Streptomyces kanamyceticus = Streptomyces coeruleoprunus > Streptomyces rimosus > Streptomyces sp. > Amy colatopsis mediterranei. Ten antibiotic combinations were used to isolate antibiotic-resistant mutants of B. japonicum I-110 and 3I1B-110 via successive cycles of mutation. Eighty-one antibiotic-resistant strains were isolated and tested for symbiotic competency; nine of which were selected for further characterization in a greenhouse pot study. Few differences in nodule number were caused by these treatments. Nodule occupancy varied from 0% to 18.3% when antibiotic-resistant strains of B. japonicum were used as the sole inoculants. However, when three mutant strains of B. japonicum were co-inoculated with S. kanamyceticus, significant increases in nodule occupancy (up to 55%) occurred. Increases in shoot nitrogen composition (27.1%–40.9%) were also caused by co-inoculation with S. kanamyceticus. Key words: Bradyrhizobium japonicum, Streptomyces kanamyceticus, indigenous bradyrhizobia, co-inoculation, nodule occupancy.

Phage coating of soybean seed reduces nodulation by indigenous soil bradyrhizobia

1992 ◽  
Vol 38 (12) ◽  
pp. 1264-1269 ◽  
Author(s):  
H. Abdel Basit ◽  
J. Scott Angle ◽  
S. Salem ◽  
E. M. Gewaily
Keyword(s):  
Glycine Max ◽  
Bradyrhizobium Japonicum ◽  
Nitrogenase Activity ◽  
Soybean Seed ◽  
Nodule Occupancy ◽  
Field Soil ◽  
Control Value ◽  
Glycine Max L ◽  
Inoculum Strain ◽  
Plant Basis

Inoculation of soybean with Bradyrhizobium japonicum is often unsuccessful owing to the failure of inoculum strains to nodulate soybeans (Glycine max (L.) Merr.) in the presence of indigenous strains of rhizobia in soil. Previous studies have shown that it is possible to reduce nodulation with indigenous strains of rhizobia by amending the soil with a bacteriophage specific for the indigenous strain. The objective of the current study was to determine whether the coating of seed with phage affected nodule occupancy and soybean growth. A phage specific for B. japonicum USDA 469 and a symbiotically superior strain of rhizobium (B. japonicum USDA 110) were coated together onto soybean seed and planted into both greenhouse and field soil previously inoculated with B. japonicum USDA 469. The phage coated onto seed reduced nodulation by B. japonicum USDA 469 to 48% occupancy, compared with 64% for the untreated control value. Nodulation by the superior inoculum strain was increased from 48 to 82% occupancy by coating seed with the homologous phage and B. japonicum USDA 110. The rate of nitrogenase activity (on a per plant basis) was increased by coating seed with the phage and B. japonicum USDA 110. No other plant or symbiotic parameters were affected by phage coating of seed. These results indicate that the nodulation of soybeans can be significantly affected by the coating of seed with phage specific for undesirable strains of rhizobia in soil and the concurrent coating of seed with desirable strains of rhizobia. Key words: competition, rhizobiophage, rhizobia, soybeans.

Sampling to determine nodule occupancy of soybean (Glycine max (L) Merr.) by Bradyrhizobium japonicum strains

1998 ◽  
Vol 44 (8) ◽  
pp. 795-799 ◽  
Author(s):  
Cécile Revellin ◽  
Jean-Baptiste Denis ◽  
Gérard Catroux
Keyword(s):  
Glycine Max ◽  
Confidence Interval ◽  
Bradyrhizobium Japonicum ◽  
Sampling Strategy ◽  
Nodule Occupancy ◽  
Experimental Conditions ◽  
Anova Model ◽  
Glycine Max L ◽  
Approximate Confidence Intervals ◽  
Time Required

The confidence interval of the percentage of soybean (Glycine max. (L.) Merr.) nodule occupancy by two strains of Bradyrhizobium japonicum was studied using samples of nodules per plant varying by multiples of 8 (8-56). These samples were recovered from all of the nodules of four sets of 21-24 plants grown in soil colonized only by these strains and typed using strain-specific sera. Approximate confidence intervals (ACI) were estimated (from the ANOVA model) using the formula, length of ACI = 4 [(0.040/P) + (0.278/PN)]1/2, where P is the number of sampled plants and N is the number of nodules characterised for each plant. The estimated ACI decreased when the number of plants sampled increased and decreased to a lesser extent when the number of nodules typed per plant increased. It was possible to define a plant and nodule sampling strategy to minimize the labor and time required to get acceptable ACI values. Under our experimental conditions, when 192 nodules were typed, combinations of 24 plants × 8 nodules per plant and 32 plants × 6 nodules per plant were the most satisfactory, corresponding to calculated ACI of 22.3 and 20.8%, respectively.Key words: Bradyrhizobium japonicum, competition, method, nodule occupancy, soybean.

Competition for nodule occupancy of introduced Bradyrhizobium japonicum strain SMGS1 in French soils already containing Bradyrhizobium japonicum strain G49

1993 ◽  
Vol 39 (11) ◽  
pp. 1022-1028 ◽  
Author(s):  
X. Pinochet ◽  
F. Arnaud ◽  
J. C. Cleyet-Marel
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Field Experiments ◽  
Physiological State ◽  
Viable Cell ◽  
Field Trials ◽  
Nodule Occupancy ◽  
Glycine Max L ◽  
Interstrain Competition ◽  
Inoculation Technology ◽  
Japonicum Strain

The competitiveness of Bradyrhizobium japonicum strains G49 and SMGS1 was first studied in the greenhouse in sterilized sand, with or without added soil. Strain SMGS1 was more competitive than strain G49 with soybean (Glycine max L.) cultivar Labrador but the two strains showed equivalent competitiveness with cultivar Kingsoy. When soil was added, nodule occupancy of strain G49 was only 22% with this cultivar. In field experiments, conducted over 2 years in soils already containing strain G49 (1.5 × 103 to 4.0 × 104 cells/g of soil), nodule occupancy of inoculated strain SMGS1 ranged from 20 to 90%. Nodule occupancy was 3–22% higher when inoculation was done by peat seed coating or with liquid inoculation in the row than with peat-coated clay microgranulars. Nodule occupancy was also dependent on the physiological state of the inoculated cells. When an inoculum stored at 28 °C for 1 year was used at the same viable cell rate, nodule occupancy of strain SMGS1 was 4–20% lower than with a recently made inoculum. Pot experiments with soil from field experiments carried out in the 1st year showed that the inoculated strain continued forming nodules without further inoculation, with a recovery rate equivalent to that of field experiment in the previous year.Key words: Bradyrhizobium japonicum, interstrain competition, inoculation technology, ELISA, field trials.

Out of War

2018 ◽  
Author(s):  
Mariane C. Ferme
Keyword(s):  
Sierra Leone ◽  
Red Cross ◽  
Cultural Survival ◽  
Social Fabric ◽  
Rural Landscapes ◽  
Republic Of China ◽  
Liminal Spaces ◽  
Humanitarian Interventions ◽  
Sensory History ◽  
History Of

Out of War is an ethnographic engagement with the nature of intercommunal violence and the material returns of history during and after the 1991–2002 Sierra Leone civil war. The questions raised concern the nature and reckoning of time and reality, fact and fiction; the experience of violence and trauma; the reversibility of perpetrator and victim, friend and enemy; and past, present, and future in the colony and postcolony. The book is a reflection on West African epistemologies and ontologies that contribute to questions in counterpoint with those of international humanitarianism, struggling with the possibilities of truth and quandaries of justice. In the context of massive population displacements and humanitarian interventions, the ethnography traces strategies of psychological, political, and cultural survival and material dwelling in liminal spaces in the midst of the destruction of the social fabric engendered by war. It also examines the juridical creation of new figures of crimes against humanity at the Special Court for Sierra Leone. The Sierra Leone scene, in the aftermath of war, is visualized as a landscape of chronotopes, neologisms that summon the uncertainty of war: the sobel (“soldier by day, rebel by night”), pointing to the instability of distinctions between enemy and friend, or of opposing parties in the war (the rebels of the Revolutionary United Front [RUF] and soldiers in the national army), and the rebel cross, pointing to the possibility that the purported neutrality of the Red Cross masked partisan interests alongside the RUF. Chronotopes also testify to the difficulty of discerning between facts and rumors in war, and they freeze in time collective anxieties about wartime events. Finally, beyond the traumas of war, the book explores the returns of material traces in counterpoint to the more “monumental” presence of Chinese investments in Africa today, and it explores the forgotten sensory history of another China (Taiwan versus the People’s Republic of China) and another Africa inscribed in ordinary agrarian practices on rural landscapes, and in the fabric of domestic life, particularly since the non-aligned movement emerged from the Bandung conference in 1955.

Making It Count

2020 ◽  
Author(s):  
Arunabh Ghosh
Keyword(s):  
Cold War ◽  
Statistical Data ◽  
New Technology ◽  
Political Leaders ◽  
Republic Of China ◽  
Great Leap Forward ◽  
History Of ◽  
History Of Statistics ◽  
The Government ◽  
The Great Leap Forward

In 1949, at the end of a long period of wars, one of the biggest challenges facing leaders of the new People's Republic of China was how much they did not know. The government of one of the world's largest nations was committed to fundamentally reengineering its society and economy via socialist planning while having almost no reliable statistical data about their own country. This book is the history of efforts to resolve this “crisis in counting.” The book explores the choices made by political leaders, statisticians, academics, statistical workers, and even literary figures in attempts to know the nation through numbers. It shows that early reliance on Soviet-inspired methods of exhaustive enumeration became increasingly untenable in China by the mid-1950s. Unprecedented and unexpected exchanges with Indian statisticians followed, as the Chinese sought to learn about the then-exciting new technology of random sampling. These developments were overtaken by the tumult of the Great Leap Forward (1958–1961), when probabilistic and exhaustive methods were rejected and statistics was refashioned into an ethnographic enterprise. By acknowledging Soviet and Indian influences, the book not only revises existing models of Cold War science but also globalizes wider developments in the history of statistics and data. Anchored in debates about statistics and its relationship to state building, the book offers fresh perspectives on China's transition to socialism.

Metabolic Adjustment of Glycine max (L.) Merril in the Presence of Nitrate and Bradyrhizobium japonicum

Agronomy ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1518
Author(s):  
Alberto Mongolo Júnior ◽  
Felipe Girotto Campos ◽  
Gustavo Ribeiro Barzotto ◽  
Jonas Akenaton Venturineli Pagassini ◽  
Maria Aparecida Ribeiro Vieira ◽  
...  
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Dry Mass ◽  
Antioxidant Systems ◽  
Oxygen Species ◽  
Membrane Stabilization ◽  
Total Sugars ◽  
Vegetative Phase ◽  
Glycine Max L ◽  
Metabolic Adjustment ◽  
Plant Dry Mass

Reactive oxygen species are generated during the processes of photosynthesis and nitrate reduction, which can compromise the integrity of biomolecules and membranes. During the vegetative phase of Fabaceae species, around half of translocated carbohydrate is used for nodule growth, while the other half returns to the aerial part with nitrogen incorporated. These sugars may be yet involved with membrane stabilization, signaling, and activation of important genetic pathways for plant development. Thus, the aim was to study the adjustments of the photosynthetic and antioxidant systems and the accumulation of carbohydrates and biomass in Glycine–Bradyrhizobium cultivated with nitrate (NO3−). Four treatments were evaluated in completely randomized blocks. Glycine–Bradyrhizobium was grown with 1.7 mM of NO3− (GB: 1.7 mM NO3−) and without NO3− (GB: 0 mM NO3−), and Glycine was grown with 1.7 mM of NO3− (G: 1.7 mM NO3−) and without NO3− (G: 0 mM NO3−). Glycine–Bradyrhizobium symbiosis contributes to photosynthetic metabolism and total sugars, reduces the action of antioxidant enzymes, and minimizes the use of nitrate in soybean cultivation.; Glycine–Bradyrhizobium with nitrate provided greater plant dry mass in the vegetative phase, along with increased enzymatic activity and reduced nodule mass.

scholarly journals Response of Soybean (Glycine max (L.) Merrill) to Mineral Nitrogen Fertilization and Bradyrhizobium japonicum Seed Inoculation

Agronomy ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1300
Author(s):  
Janusz Prusiński ◽  
Anna Baturo-Cieśniewska ◽  
Magdalena Borowska
Keyword(s):  
Nitrogen Fertilization ◽  
Bradyrhizobium Japonicum ◽  
Plant Density ◽  
Higher Plants ◽  
High Sensitivity ◽  
N Fertilization ◽  
Mineral N ◽  
Plant Nitrogen ◽  
Seed Inoculation ◽  
Glycine Max L

A growing interest in soybean cultivation in Poland has been observed in the recent years, however it faces a lot of difficulties resulting from a poorly understood effectiveness of plant nitrogen fertilization and from the introduction of Bradyrhizobium japonicum to the environment. The aim of the study was to evaluate the consistency of response of two soybean cultivars to three different rates of mineral N fertilization and two seed inoculation treatments with B. japonicum in field conditions over four years regardless of previous B. japonicum presence in the soil. A highly-diversified-over-years rainfall and temperature in the growing season do not allow for a definite statement of the differences resulting from seed inoculation and mineral N fertilization applied separately or jointly in soybean. A high sensitivity of the nodulation process to rainfall deficits was noted, which resulted in a decreased amount of B. japonicum DNA measured in qPCR and dry matter of nodules. ‘Annushka’ demonstrated a higher yield of seeds and protein, higher plants and the 1st pod setting. ‘Aldana’, due to a significant decrease in plant density, produced a higher number of pods, seeds per pod and the 1000 seed weight per plant. Both cultivars responded with an increase in the seed yield after seed inoculation with HiStick, also with an application of 30 and 60 kg N, as well as with Nitragina with 60 kg N.

Surviving Troubled Times on the Borders of China: Sheng Shicai as a Successful Warlord in Xinjiang, 1931–34

2021 ◽  
pp. 1-40
Author(s):  
Yuan Zhi Ou
Keyword(s):  
Professional Training ◽  
Military Training ◽  
Military Officers ◽  
Republic Of China ◽  
Government Documents ◽  
Frontier Region ◽  
Chinese Military ◽  
History Of ◽  
The Republic ◽  
Early Republican

Abstract Ethnicity, religion, and geopolitics affect historians’ interpretations of the history of Xinjiang, a very chaotic frontier region of China that did not come fully under the control of the People’s Republic of China until recent decades. The case of Sheng Shicai, an early Republican Era Chinese military officer, shows how professional training and, most importantly, the ability to capitalize on emerging opportunities contributed to his military success in Xinjiang from 1931 to 1934. This paper analyzes the Republic of China’s government documents, Sheng and his acquaintances’ memoirs, newspaper articles, and other sources to examine how Sheng applied his military training and employed regional and foreign military forces to win battles in northern Xinjiang. Professional military training helped officers to utilize their resources efficiently and take advantage of their geopolitical situations. Amid numerous talented Chinese military officers, Sheng rose in rank and successfully secured Xinjiang as a part of the Republic of China even when Xinjiang’s geopolitics seemed extremely challenging. This study highlights the value of Sheng’s military prowess, something that the literature has not previously appreciated.

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