Phospholipid metabolism in the molluscs. I. Distribution of phospholipids in the water snail Lymnaea stagnalis

1969 ◽  
Vol 47 (2) ◽  
pp. 85-89 ◽  
Author(s):  
Chi-Rong Liang ◽  
K. P. Strickland
Keyword(s):  
Thin Layer ◽  
Glyceryl Ether ◽  
Lymnaea Stagnalis ◽  
Phospholipid Metabolism ◽  
Qualitative And Quantitative ◽  
Ethanolamine Phosphoglycerides ◽  
Phospholipid Distribution ◽  
Layer Chromatography ◽  
Derivatives Of ◽  
Very High

The phospholipid distribution in the mollusc Lymnaea stagnalis was investigated by fractionation of the lipid extract with silica-gel column chromatography followed by qualitative and quantitative thin-layer chromatography. The results obtained show that choline and ethanolamine phosphoglycerides are the major phospholipids in this species and that they amount to about 50% and 30% of total phospholipids, respectively. The amount of ceramide 2-aminoethylphosphonate in this species is 7.5% of the total phospholipids and this compound is the main phosphonolipid in this species. The glyceryl ether derivatives of both choline and ethanolamine phosphoglycerides are found to be very high. Nearly half (45%) of the choline phosphoglycerides is glyceryl ether in nature while 15% of the ethanolamine phosphoglycerides is also of this type.

scholarly journals The reaction of iodobenzene-p-sulphonyl chloride (pipsyl chloride) with certain amino acids and peptides, and with insulin

1967 ◽  
Vol 102 (3) ◽  
pp. 815-824 ◽  
Author(s):  
J. C. Fletcher
Keyword(s):  
Amino Acids ◽  
Thin Layer ◽  
Acid Hydrolysis ◽  
Thin Layer Chromatography ◽  
Cysteic Acid ◽  
Buffer System ◽  
The Common ◽  
Layer Chromatography ◽  
Derivatives Of

1. A system of separation using buffered Celite columns is described that enables the pipsyl derivatives of most of the common amino acids to be separated. 2. The reaction of pipsyl chloride with several amino acids not included in previous studies has been investigated. In particular, knowledge of the acid-soluble pipsyl derivatives of arginine, histidine, lysine, tyrosine and cysteic acid has been extended. 3. Reproducible factors have been obtained that enable corrections to be applied for the breakdown of pipsylamino acids on acid hydrolysis. 4. The reaction of pipsyl chloride with peptides has been studied under various conditions. 5. The extent of the reaction between pipsyl chloride and insulin depends on the nature of the solvent-buffer system, and under the best conditions so far found is about 75% complete. 6. In an Appendix, the separation of pipsylamino acids by thin-layer chromatography is described.

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