Simultaneous qualitative and quantitative analyses of ursolic acid and oleanolic acid in Punica granatum L. (Pomegranate) flowers by high-performance thin-layer chromatography

2021 ◽  
Author(s):  
Wenxia Wu ◽  
Liwa Wang ◽  
Shuge Tian
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Ursolic Acid ◽  
Oleanolic Acid ◽  
High Performance ◽  
Punica Granatum ◽  
Qualitative And Quantitative ◽  
Quantitative Analyses ◽  
Layer Chromatography ◽  
Punica Granatum L

scholarly journals Quantification of Eugenol, Luteolin, Ursolic Acid, and Oleanolic Acid in Black (Krishna Tulasi) and Green (Sri Tulasi) Varieties of Ocimum sanctum Linn. Using High-Performance Thin-Layer Chromatography

2006 ◽  
Vol 89 (6) ◽  
pp. 1467-1474 ◽  
Author(s):  
Sheetal Anandjiwala ◽  
Jyoti Kalola ◽  
Mandapati Rajani
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Ursolic Acid ◽  
Oleanolic Acid ◽  
High Performance ◽  
Ocimum Sanctum ◽  
Relative Standard ◽  
Recovery Study ◽  
Acid Reagent ◽  
Layer Chromatography

Abstract Ocimum sanctum (family Lamiaceae) is a reputed drug of Ayurveda, commonly known as Tulasi. Inthe present work, we quantified 4 marker compounds, viz., eugenol, luteolin, ursolic acid, and oleanolic acid, from the leaf of green and black varieties of O. sanctum using high-performance thin-layer chromatography (HPTLC) with densitometry. The methods were found to be precise, with relative standard deviation (RSD) values for intraday analyses in the range of 0.52 to 0.91%, 0.77 to 1.29%, 0.11 to 0.16%, and 0.34 to 0.42% and for interday analyses in the range of 0.73 to 0.96%, 1.02 to 2.08%, 0.11 to 0.12%, and 0.39 to 0.64% for different concentrations of eugenol, luteolin, ursolic acid, and oleanolic acid, respectively. Instrumental RSD values were 0.24, 0.39, 0.21, and 0.18% for eugenol, luteolin, ursolic acid, and oleanolic acid, respectively. Accuracy of the methods was checked by conducting a recovery study at 3 different levels for the 4 compounds, and the average recoveries were found to be 99.73, 99.3, 100.58, and 100.57%, respectively. Eugenol content ranged from 0.175 to 0.362% (w/w) and luteolin from 0.019 to 0.046% (w/w) in the samples analyzed. Green variety was found to contain higher amounts of ursolic acid [0.478 and 0.348% (w/w), from Sources 1 and 2, respectively] than the black variety [0.252 and 0.264% (w/w) from Sources 1 and 2, respectively]. Black variety had 0.174 and 0.218% (w/w) of oleanolic acid from Sources 1 and 2, respectively, while it was not detected in the green variety. Ursolic acid and oleanolic acid ran at the same Rf value and could not be resolved in several solvent systems tried. However, we observed that only ursolic acid gave yellow fluorescence under 366 nm ultraviolet light after derivatization with anisaldehydesulfuric acid reagent. The HPTLC-densitometry methods for the quantification of the 4 markers in O. sanctum leaf will have the applicability in quality control.

scholarly journals Determination of Ursolic Acid a Biomarker in Different Swertia Species through High Performance Thin Layer Chromatography

2011 ◽  
Vol 02 (04) ◽  
pp. 121-124 ◽  
Author(s):  
Mansi Gupta ◽  
Deepa Bisht ◽  
Sayyada Khatoon ◽  
Sharad Srivastava ◽  
Ajay Kumar Singh Rawat
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Ursolic Acid ◽  
High Performance ◽  
Swertia Species ◽  
Layer Chromatography
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