Structural studies of the class II histocompatibility antigens of the ACI rat

1985 ◽  
Vol 63 (12) ◽  
pp. 1241-1249
Author(s):  
Audrey J. Goldner-Sauvé ◽  
Abraham Fuks ◽  
Ronald D. Guttmann
Keyword(s):  
Gel Electrophoresis ◽  
Class Ii ◽  
Gene Products ◽  
Structural Studies ◽  
Cell Binding ◽  
Binding Studies ◽  
Histocompatibility Antigens ◽  
Two Dimensional Gel Electrophoresis ◽  
Aci Rat ◽  
Class Ii Antigens

The class II antigens of the ACI rat were studied using both conventional alloantisera and monoclonal antibodies. By sequential immunoprecipitation experiments and cell binding studies, alloantisera were shown to contain antibodies to both the RT1.B and the RT1.D gene products. Using one- and two-dimensional gel electrophoresis, the structures of these gene products were shown to be distinguishable. The importance of these differences for the immune response and antigen presentation is discussed.

scholarly journals Evidence for two homologous, but nonidentical, Ia molecules determined by the I-EC subregion.

1979 ◽  
Vol 150 (1) ◽  
pp. 100-107 ◽  
Author(s):  
T L Delovitch ◽  
B H Barber
Keyword(s):  
Staphylococcus Aureus ◽  
Gel Electrophoresis ◽  
Peptide Mapping ◽  
Molecular Size ◽  
Gene Products ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis ◽  
Partial Digestion

Sequential immunoprecipitation, two-dimensional gel electrophoresis and peptide mapping analyses of B10A(3R), 35S-methionine-labeled, I-EC subregion products were performed. Evidence is presented here for the presence of two structurally homologous, but nonidentical, gene products of the I-EC subregion. These two Ia molecules are independently immunoprecipitable, identical in molecular size and charge, but differ by approximately equal to 20% in their peptides obtained by partial digestion with Staphylococcus aureus protease V8.

scholarly journals Two-dimensional gel electrophoretic analysis of the MT3 and DR4 molecules from the different D-typed cells.

1984 ◽  
Vol 160 (3) ◽  
pp. 751-758 ◽  
Author(s):  
M Suzuki ◽  
T Yabe ◽  
M Satake ◽  
T Juji ◽  
H Hamaguchi
Keyword(s):  
Light Chain ◽  
Gel Electrophoresis ◽  
Electrophoretic Analysis ◽  
Structural Heterogeneity ◽  
Class Ii ◽  
Light Chains ◽  
Two Dimensional ◽  
Structural Polymorphism ◽  
Two Dimensional Gel Electrophoresis

This report demonstrates directly, using two-dimensional gel electrophoresis and alloantisera, the following: (a) The DR4 light chains show a structural polymorphism among the Dw4, DKT2, and DYT cells. (b) Most of the class II light chains consist of the DR light chain. (c) The MT3 molecule is distinct from the DR4 molecule in the Dw4, DKT2, and DYT cells. (d) The MT3 molecule does not show any structural heterogeneity among the Dw4, DKT2, and DYT cells. These results suggest that the dissection of the D specificity among Dw4, DKT2, and DYT is mainly caused by the differences of the DR4 molecules.

Structural studies and two-dimensional gel electrophoresis of γ-glutamyl transpeptidase

1986 ◽  
Vol 247 (1) ◽  
pp. 165-170 ◽  
Author(s):  
James L. Cone ◽  
Irene B. Glowinski ◽  
Peter J. Wirth ◽  
Preston H. Grantham ◽  
Peter P. Roller
Keyword(s):  
Gel Electrophoresis ◽  
Two Dimensional ◽  
Structural Studies ◽  
Two Dimensional Gel Electrophoresis ◽  
Glutamyl Transpeptidase

An approach to molecular analysis of T-cell clones by two-dimensional gel electrophoresis: is there interclonal and intraclonal heterogeneity?

1984 ◽  
Vol 30 (12) ◽  
pp. 1950-1955 ◽  
Author(s):  
J Kettman ◽  
I Lefkovits
Keyword(s):  
T Cell ◽  
Gel Electrophoresis ◽  
T Cell Subsets ◽  
Large Set ◽  
Gene Products ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis ◽  
T Cell Clones ◽  
T Effector Cells ◽  
Cell Clones

Abstract To understand the regulation of immunity one must understand the processes and the consequences of the activation of the lymphoid cell, its clonal proliferation, and the ultimate differentiation of the progeny into the effector elements that constitute the immune system. By use of polyclonal mitogens, individual T-cell precursors can be activated for study of their clonal growth and generation of T effector cells. We used two-dimensional gel electrophoresis to study the gene products expressed by such T-cell clones. On comparing the radiofluorograms of such gels for a large set of T-cell clones we find that many gene products are expressed in common, as expected, but that another large set of products varies from one clone to the other and might be designated as markers of T-cell subsets or stages of differentiation. By subdividing the growing clone and analyzing the family of daughter clones, we find considerable variation in gene expression during clonal expansion.

Diversity of Glycoprotein Deficiencies in Glanzmann’s Thrombasthenia

1985 ◽  
Vol 54 (03) ◽  
pp. 626-629 ◽  
Author(s):  
M Meyer ◽  
F H Herrmann
Keyword(s):  
High Resolution ◽  
Gel Electrophoresis ◽  
Type Ii ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis ◽  
Structural Variant ◽  
Glanzmann’S Thrombasthenia ◽  
Crossed Immunoelectrophoresis ◽  
Glanzmann's Thrombasthenia ◽  
Platelet Proteins

SummaryThe platelet proteins of 9 thrombasthenic patients from 7 families were analysed by high resolution two-dimensional gel electrophoresis (HR-2DE) and crossed immunoelectrophoresis (CIE). In 7 patients both glycoproteins (GPs) IIb and Ilia were absent or reduced to roughly the same extent. In two related patients only a trace of GP Ilb-IIIa complex was detected in CIE, but HR-2DE revealed a glycopeptide in the position of GP Ilia in an amount comparable to type II thrombasthenia. This GP Ilia-like component was neither recognized normally by anti-GP Ilb-IIIa antibodies nor labeled by surface iodination. In unreduced-reduced two-dimensional gel electrophoresis two components were observed in the region of GP Ilia. The assumption of a structural variant of GP Ilia in the two related patients is discussed.

Comparative Two-Dimensional Gel Electrophoresis of Trypanosoma cruzi Mammalian-Stage Forms in an Alkaline pH Range

2015 ◽  
Vol 22 (12) ◽  
pp. 1066-1075 ◽  
Author(s):  
Adriana Magalhães ◽  
Rayner Queiroz ◽  
Izabela Bastos ◽  
Jaime Santana ◽  
Marcelo Sousa ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Gel Electrophoresis ◽  
Two Dimensional ◽  
Alkaline Ph ◽  
Two Dimensional Gel Electrophoresis ◽  
Ph Range
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