OXIDATION OF THE ANALYTICAL REAGENT "TIRON" (DISODIUM-4,5-DIHYDROXYBENZENE-1,3-DISULPHONATE)

1957 ◽  
Vol 35 (5) ◽  
pp. 477-487 ◽  
Author(s):  
G. F. Atkinson ◽  
W. A. E. McBryde
Keyword(s):  
Alkaline Solution ◽  
Oxidation Product ◽  
Alkaline Solutions ◽  
Colorimetric Determination ◽  
Analytical Reagent ◽  
Do So

The analytical reagent "tiron" undergoes oxidation in alkaline solution by the oxygen of the atmosphere to a yellow-colored substance, which is assumed to be the corresponding quinone. In certain circumstances the reagent may be oxidized with production of a green substance believed to be a semiquinone. Interference in the colorimetric determination of iron by means of this reagent may occur in alkaline solution owing to the overlap of the iron III derivative and of the oxidation product. Alkaline solutions of tiron react with iron II salts even in the rigorous absence of oxygen to form the iron III tironate FeR3−9. Oxygenated solutions of tiron in alkali fail to produce this compound with iron III salts, but do so with iron II salts.

Colorimetric Determination of Sympathomimetic Amines Methyldopa and Noradrenaline

1985 ◽  
Vol 68 (1) ◽  
pp. 91-95
Author(s):  
Mohamed I Walash ◽  
Abed Abou Ouf ◽  
Fatma B Salem
Keyword(s):  
Alkaline Solution ◽  
Dosage Forms ◽  
Colorimetric Determination ◽  
Sympathomimetic Amines ◽  
Chromogenic Reagent ◽  
Beer's Law ◽  
Beer’S Law ◽  
Orange Color

Abstract The chromogenic reagent p-dimethylaminocinnamaldehyde (PDAC) is introduced for the determination of the sympathomimetic amines methyldopa and noradrenaline. The method is based on measurement of the orange color developed when the alkaline solution of methyldopa and noradrenaline is allowed to react with PDAC at pH 5.0. The color developed obeys Beer's law in the concentration range 0.1-1.5 mL of 2 x 103M solution of noradrenaline and methyldopa. The results are compared with those obtained with another chromogenic reagent, pdimethylaminobenzaldehyde (PDAB). Determinations on dosage forms of the drugs, using PDAC and PDAB reagents, agreed well with results of determinations by official pharmacopoeia! methods.

Automated Method for the Determination of Serum Creatine Kinase Activity

1967 ◽  
Vol 13 (3) ◽  
pp. 233-241 ◽  
Author(s):  
Gerard A Fleisher
Keyword(s):  
Creatine Kinase ◽  
Alkaline Solution ◽  
Kinase Activity ◽  
Adenosine Diphosphate ◽  
Creatine Phosphate ◽  
Creatine Kinase Activity ◽  
Serum Creatine Kinase ◽  
Colorimetric Determination ◽  
Automated Method

Abstract An automated (AutoAnalyzer) method for the colorimetric determination of creatine kinase activity in serum is described. This method includes reactivation of creatine kinase with cysteine, incubation of the active enzyme with creatine phosphate and adenosine diphosphate at 37.5°, and subsequent inactivation of enzyme and binding of cysteine by phenylmercuric borate. The enzymatically produced creatine is dialyzed against a solution of diacetyl and reacted with α-naphthol in an alkaline solution. The absorbance of the colored end product is measured at 550 mµ. Individual blanks are determined in the absence of adenosine diphosphate. Comparison of results obtained by this method and a manual procedure shows satisfactory agreement.

Improved Procedure for the Colorimetric Determination of Sulfamethazine in Feeds Containing Aureo SP-250®

1965 ◽  
Vol 48 (5) ◽  
pp. 905-908
Author(s):  
T R Berg ◽  
J Q Penrod ◽  
L G Blaylock
Keyword(s):  
Alkaline Solution ◽  
Extraction Method ◽  
Colorimetric Determination ◽  
Alkaline Extraction ◽  
Separate Study

Abstract The procedure provided by the manufacturer for the colorimetric determination of sulfamethazine in feeds uses a hot alkaline extraction. This extract is difficult to handle because many feeds form a gel and solubilized protein when heated in the presence of an alkaline solution. Centrifugation does not readily separate these solids. An extraction method utilizing a cold alkaline extraction and a double centrifugation has been developed which yields an extract that can be readily carried through the remaining steps of the analysis. Average recoveries were 104% at the 0.01% level and 106% at the 0.05% level of sulfamethazine. In addition, a separate study was conducted to determine whether penicillin or aureomycin interfere with the analysis for sulfamethazine. The data indicate that there is no interference.

A NOTE ON THE COLORIMETRIC DETERMINATION OF PROLINE

1939 ◽  
Vol 17b (4) ◽  
pp. 143-144 ◽  
Author(s):  
G. H. Guest
Keyword(s):  
Oxidation Product ◽  
Steam Distillation ◽  
Colorimetric Method ◽  
Colorimetric Determination ◽  
Colour Reaction

A colorimetric method for the determination of proline is described. The procedure involves the oxidation of proline with lead peroxide and the condensation of the oxidation product with p-dimethylaminobenzaldehyde to give a red compound. The oxidation product can, if desired, be separated by steam distillation before applying the colour reaction. The method is limited in application to proteins which contain little or no hydroxyproline. By this method casein is found to contain 7.94% of proline.

A Method for the Colorimetric Determination of β-Glucuronidase in Urine, Serum, Tissue; Assay of Enzymatic Activity in Health and Disease

1959 ◽  
Vol 36 (2) ◽  
pp. 193-201 ◽  
Author(s):  
Julius A. Goldbarg ◽  
Esteban P. Pineda ◽  
Benjamin M. Banks ◽  
Alexander M. Rutenburg
Keyword(s):  
Enzymatic Activity ◽  
Colorimetric Determination ◽  
Health And Disease ◽  
Urine Serum
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