Selective colorimetric determination of TNT partitioned between an alkaline solution and a strongly basic Dowex 1-X8 anion exchanger

2008 ◽  
Vol 174 (2-3) ◽  
pp. 239-243 ◽  
Author(s):  
Ayşem Üzer ◽  
Erol Erçağ ◽  
Reşat Apak
Keyword(s):  
Alkaline Solution ◽  
Anion Exchanger ◽  
Colorimetric Determination ◽  
Dowex 1

Colorimetric Determination of Sympathomimetic Amines Methyldopa and Noradrenaline

1985 ◽  
Vol 68 (1) ◽  
pp. 91-95
Author(s):  
Mohamed I Walash ◽  
Abed Abou Ouf ◽  
Fatma B Salem
Keyword(s):  
Alkaline Solution ◽  
Dosage Forms ◽  
Colorimetric Determination ◽  
Sympathomimetic Amines ◽  
Chromogenic Reagent ◽  
Beer's Law ◽  
Beer’S Law ◽  
Orange Color

Abstract The chromogenic reagent p-dimethylaminocinnamaldehyde (PDAC) is introduced for the determination of the sympathomimetic amines methyldopa and noradrenaline. The method is based on measurement of the orange color developed when the alkaline solution of methyldopa and noradrenaline is allowed to react with PDAC at pH 5.0. The color developed obeys Beer's law in the concentration range 0.1-1.5 mL of 2 x 103M solution of noradrenaline and methyldopa. The results are compared with those obtained with another chromogenic reagent, pdimethylaminobenzaldehyde (PDAB). Determinations on dosage forms of the drugs, using PDAC and PDAB reagents, agreed well with results of determinations by official pharmacopoeia! methods.

OXIDATION OF THE ANALYTICAL REAGENT "TIRON" (DISODIUM-4,5-DIHYDROXYBENZENE-1,3-DISULPHONATE)

1957 ◽  
Vol 35 (5) ◽  
pp. 477-487 ◽  
Author(s):  
G. F. Atkinson ◽  
W. A. E. McBryde
Keyword(s):  
Alkaline Solution ◽  
Oxidation Product ◽  
Alkaline Solutions ◽  
Colorimetric Determination ◽  
Analytical Reagent ◽  
Do So

The analytical reagent "tiron" undergoes oxidation in alkaline solution by the oxygen of the atmosphere to a yellow-colored substance, which is assumed to be the corresponding quinone. In certain circumstances the reagent may be oxidized with production of a green substance believed to be a semiquinone. Interference in the colorimetric determination of iron by means of this reagent may occur in alkaline solution owing to the overlap of the iron III derivative and of the oxidation product. Alkaline solutions of tiron react with iron II salts even in the rigorous absence of oxygen to form the iron III tironate FeR3−9. Oxygenated solutions of tiron in alkali fail to produce this compound with iron III salts, but do so with iron II salts.

Automated Method for the Determination of Serum Creatine Kinase Activity

1967 ◽  
Vol 13 (3) ◽  
pp. 233-241 ◽  
Author(s):  
Gerard A Fleisher
Keyword(s):  
Creatine Kinase ◽  
Alkaline Solution ◽  
Kinase Activity ◽  
Adenosine Diphosphate ◽  
Creatine Phosphate ◽  
Creatine Kinase Activity ◽  
Serum Creatine Kinase ◽  
Colorimetric Determination ◽  
Automated Method

Abstract An automated (AutoAnalyzer) method for the colorimetric determination of creatine kinase activity in serum is described. This method includes reactivation of creatine kinase with cysteine, incubation of the active enzyme with creatine phosphate and adenosine diphosphate at 37.5°, and subsequent inactivation of enzyme and binding of cysteine by phenylmercuric borate. The enzymatically produced creatine is dialyzed against a solution of diacetyl and reacted with α-naphthol in an alkaline solution. The absorbance of the colored end product is measured at 550 mµ. Individual blanks are determined in the absence of adenosine diphosphate. Comparison of results obtained by this method and a manual procedure shows satisfactory agreement.

Improved Procedure for the Colorimetric Determination of Sulfamethazine in Feeds Containing Aureo SP-250®

1965 ◽  
Vol 48 (5) ◽  
pp. 905-908
Author(s):  
T R Berg ◽  
J Q Penrod ◽  
L G Blaylock
Keyword(s):  
Alkaline Solution ◽  
Extraction Method ◽  
Colorimetric Determination ◽  
Alkaline Extraction ◽  
Separate Study

Abstract The procedure provided by the manufacturer for the colorimetric determination of sulfamethazine in feeds uses a hot alkaline extraction. This extract is difficult to handle because many feeds form a gel and solubilized protein when heated in the presence of an alkaline solution. Centrifugation does not readily separate these solids. An extraction method utilizing a cold alkaline extraction and a double centrifugation has been developed which yields an extract that can be readily carried through the remaining steps of the analysis. Average recoveries were 104% at the 0.01% level and 106% at the 0.05% level of sulfamethazine. In addition, a separate study was conducted to determine whether penicillin or aureomycin interfere with the analysis for sulfamethazine. The data indicate that there is no interference.

A Method for the Colorimetric Determination of β-Glucuronidase in Urine, Serum, Tissue; Assay of Enzymatic Activity in Health and Disease

1959 ◽  
Vol 36 (2) ◽  
pp. 193-201 ◽  
Author(s):  
Julius A. Goldbarg ◽  
Esteban P. Pineda ◽  
Benjamin M. Banks ◽  
Alexander M. Rutenburg
Keyword(s):  
Enzymatic Activity ◽  
Colorimetric Determination ◽  
Health And Disease ◽  
Urine Serum

Colorimetric Determination of Fluoride in Drinking Groundwater using a Polymeric Zirconium Complex of 5-(2-Carboxyphenylazo)-8-Hydroxyquinoline

2013 ◽  
Vol 12 (7) ◽  
pp. 460-465
Author(s):  
Sameer Amereih ◽  
Zaher Barghouthi ◽  
Lamees Majjiad
Keyword(s):  
Drinking Water ◽  
Detection Limit ◽  
Complex Formation ◽  
Molar Absorptivity ◽  
Colorimetric Determination ◽  
Zirconium Complex ◽  
Reliable Determination ◽  
Percentage Recovery ◽  
Quantitation Limit

A sensitive colorimetric determination of fluoride in drinking water has been developed using a polymeric zirconium complex of 5-(2-Carboxyphenylazo)-8-Hydroxyquinoline as fluoride reagents. The method allowed a reliable determination of fluoride in range of (0.0-1.5) mg L-1. The molar absorptivity of the complex formation is 7695 ± 27 L mol-1 cm-1 at 460 nm. The sensitivity, detection limit, quantitation limit, and percentage recovery for 1.0 mg L-1 fluoride for the proposed method were found to be 0.353 ± 0.013 μg mL-1, 0.1 mg L-1, 0.3 mg L-1, and 101.7 ± 4.1, respectively.

Colorimetric Determination of Amoxicillin in Pure and some Pharmaceutical Formulations Via Reaction with Potassium Permanganate as Oxidant Reagent

2019 ◽  
Vol 10 (02) ◽  
Author(s):  
Abbas Shebeeb Al-kadumi ◽  
Sahar Rihan Fadhel ◽  
Mohammed Abdullah Ahmed ◽  
Luma Amer Musa
Keyword(s):  
Potassium Permanganate ◽  
Pharmaceutical Preparations ◽  
Pharmaceutical Formulations ◽  
Atomic Emission ◽  
Basic Medium ◽  
Photometric Method ◽  
Colorimetric Determination ◽  
Spectrophotometric Methods ◽  
Label Claim

We proposed two simple, rapid, and convenient spectrophotometric methods are described for the determination of Amoxicillin in bulk and its pharmaceutical preparations. They are based on the measurement of the flame atomic emission of potassium ion (in first method) and colorimetric determination of the green colored solution for manganite ion at 610 nm formed after reaction of Amoxicillin with potassium permanganate as oxidant agent (in the second method) in basic medium. The working conditions of the methods were investigated and optimized. Beer's law plot showed a good correlation in the concentration range of 5-45 μg/ml. The detection limits and relative standared deviations were (2.573, 2.814 μg/ml) (2.137, 2.498) for the flame emission photometric method and (1.844, 2.016 μg/ml) (1.645,1.932) for colorimetric methods for capsules and suspensions respectively. The methods were successfully applied to the determination of Amoxicillin in capsules and suspensions, and the obtained results were in good agreement with the label claim. No interference was observed from the commonly encountered additives and expectancies.

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