Semimicro Determination of Sugar Configuration by Measurement of Circular Dichroism of Alditol Acetates

1973 ◽  
Vol 51 (2) ◽  
pp. 324-326 ◽  
Author(s):  
G. M. Bebault ◽  
J. M. Berry ◽  
Y. M. Choy ◽  
G. G. S. Dutton ◽  
N. Funnell ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Circular Dichroism ◽  
Structural Information ◽  
Acetoxy Group ◽  
Gas Liquid Chromatography ◽  
Circular Dichroïsm ◽  
Alditol Acetates

The configuration (D or L) of a sugar may be determined conveniently by circular dichroism measurements at 213 nm on alditol acetates, or their methylated derivatives, where the acetoxy group acts as a chromophore. Only milligram quantities of material are required and the method is well suited to analyzing fractions obtained by gas–liquid chromatography. Structural information which may be derived from the c.d. spectra is briefly discussed.

Gas-Liquid Chromatographic Determination of Monosaccharides and Glycerol in Aged Distilled Spirits

1974 ◽  
Vol 57 (1) ◽  
pp. 111-117 ◽  
Author(s):  
Robert A Black ◽  
Arthur A Andreasen
Keyword(s):  
Liquid Chromatography ◽  
Total Concentration ◽  
Chromatographic Determination ◽  
Gas Liquid Chromatography ◽  
White Oak ◽  
Distilled Spirits ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic ◽  
Alditol Acetates

Abstract Gas-liquid chromatography was utilized for the analysis of monosaccharides and glycerol in distilled spirits aged in new, charred white oak cooperage. Arabinose, xylose, fructose, glucose, galactose, and rhamnose were identified. Traces of fucose and mannose were indicated. Total concentration of sugars increased from 16 to 37 g/100 L at 100° proof and glycerol from 0.83 to 2.42 g/100 L at 100° proof during aging from 1 through 12 years. Compounds were identified by a comparison of GLC chromatograms of trimethylsilyl (TMS) ether derivatives, alditol acetates and trifluoroacetates, and TMS-aldono-l,4-lactones. Sugars and glycerol were quantitated as TMS ether derivatives with 3 % JXR organic phase on Gas-Chrom Q with an accuracy assessed at ±2—3%.

Sign in / Sign up

Export Citation Format

Share Document