Chemical synthesis of GDP-fucose analogs and their utilization by the Lewis *A(1 → 4) fucosyltransferase

Chemical syntheses are reported for GDP-fucose (5), GDP-3-deoxy-fucose (6), and GDP-arabinose (7), the demethyl analog of 5. All three sugar nucleotides were found to act as donor substrates for an α(1 → 4) fucosyltransferase isolated from human milk when *BDGal(1 → 3)*BDGlcNAc-O(CH2)8COOMe (1) was used as the acceptor. The rate of transfer of sugar residues to 1 was measured using a coupled spectrophotometric assay and was found to be 100% (5), 2.3% (6), and 5.9% (7). The product Lea-active oligosaccharide analogs were identified by both an enzyme-linked immunosorbent assay (ELISA) and by 1H NMR spectroscopy. Keywords: glycosyltransferase, oligosaccharide synthesis, sugar-nucleotide analog, ELISA assay, fucosyltransferase.

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Secretory Anti-Giardia lamblia Antibodies in Human Milk: Protective Effect Against Diarrhea

PEDIATRICS ◽

10.1542/peds.93.1.28 ◽

1994 ◽

Vol 93 (1) ◽

pp. 28-31

Author(s):

Juan N. Walterspiel ◽

Ardythe L. Morrow ◽

Larry K. Pickering ◽

Guillermo M. Ruiz-Palacios ◽

M. Lourdes Guerrero

Keyword(s):

Human Milk ◽

Protective Effect ◽

Immunosorbent Assay ◽

Giardia Lamblia ◽

Diarrheal Disease ◽

Secretory Iga ◽

Enzyme Linked Immunosorbent Assay ◽

Giardia Infection ◽

Maternal Milk ◽

Milk Samples

Objective. To determine whether anti-Giardia lamblia secretory IgA (sIgA) antibodies in human milk protect infants from acquisition of or symptoms associated with Giardia infection. Methods. One hundred ninety-seven Mexican mother/infant pairs were followed weekly from birth for diarrheal disease and feeding status. Infant stool specimens were collected weekly and were cultured for bacterial pathogens and tested for Giardia and rotavirus by enzyme-linked immunosorbent assay. Maternal milk samples were collected weekly for 1 month postpartum and monthly thereafter. To determine the protective effect of anti-Giardia sIgA in milk against infection and against diarrhea due to Giardia, milk samples from mothers of infected infants and appropriately matched controls were assayed for anti-Giardia sIgA by enzyme-linked immunosorbent assay. Results. Asymptomatic, infected infants ingested significantly (P = .046) higher amounts of milk anti-Giardia sIgA compared with symptomatic, infected infants. However, milk anti-Giardia sIgA concentrations did not differ between Giardia-infected and noninfected infants. Conclusion. The amount of anti-Giardia sIgA in human milk was associated with prevention of symptoms of diarrhea due to Giardia, but not with acquisition of the organism.

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Quantification of human apolipoprotein A-IV by "sandwich"-type enzyme-linked immunosorbent assay.

Clinical Chemistry ◽

10.1093/clinchem/34.4.739 ◽

1988 ◽

Vol 34 (4) ◽

pp. 739-743 ◽

Cited By ~ 33

Author(s):

M Rosseneu ◽

G Michiels ◽

W De Keersgieter ◽

J Bury ◽

J P De Slypere ◽

...

Keyword(s):

Immunosorbent Assay ◽

Enzyme Linked Immunosorbent Assay ◽

Elisa Assay ◽

Chromatographic Fractionation ◽

Human Apolipoprotein ◽

Apolipoprotein A ◽

Sandwich Type ◽

Antibody Conjugate ◽

The Mean

Abstract A specific and sensitive "sandwich"-type enzyme-linked immunosorbent assay (ELISA) has been developed for quantifying human apo A-IV. Using apo A-IV immunosorbent columns, we isolated monospecific anti-apo A-IV antibodies for coating the ELISA plates and for preparing peroxidase-antibody conjugate. The assay can detect as little as 0.20 ng of apo A-IV, with mean intra- and interassay CVs of 3.6% and 8.2%, respectively. The apoA-IV concentrations in normolipemic and hyperlipemic plasma were unaffected by either delipidation or treatment with detergents or urea. To validate the ELISA assay we compared it with an immunoelectrophoretic technique. ApoA-IV concentrations in plasma from normo- and dyslipemic subjects compared well by the two assays (r = 0.89). The mean apo A-IV concentration, measured by ELISA in plasma from 50 normolipemic subjects, was 143 (SD 52) mg/L; values for dyslipemic subjects were not significantly different. We also used this new assay to monitor apo A-IV profiles of normolipemic and hypertriglyceridemic plasma after chromatographic fractionation.

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Detection of Campylobacter jejuni and Campylobacter coli in Environmental Waters by PCR Enzyme-Linked Immunosorbent Assay

Applied and Environmental Microbiology ◽

10.1128/aem.68.3.1319-1324.2002 ◽

2002 ◽

Vol 68 (3) ◽

pp. 1319-1324 ◽

Cited By ~ 38

Author(s):

A. D. Sails ◽

F. J. Bolton ◽

A. J. Fox ◽

D. R. A. Wareing ◽

D. L. A. Greenway

Keyword(s):

Campylobacter Jejuni ◽

Immunosorbent Assay ◽

Water Samples ◽

Enrichment Culture ◽

Environmental Water ◽

Environmental Water Samples ◽

Enzyme Linked Immunosorbent Assay ◽

Campylobacter Coli ◽

Elisa Assay ◽

Bacterial Cells

ABSTRACT A PCR enzyme-linked immunosorbent assay (ELISA) assay was applied to the detection of Campylobacter jejuni and Campylobacter coli in environmental water samples after enrichment culture. Bacterial cells were concentrated from 69 environmental water samples by using filtration, and the filtrates were cultured in Campylobacter blood-free broth. After enrichment culture, DNA was extracted from the samples by using a rapid-boiling method, and the DNA extracts were used as a template in a PCR ELISA assay. A total of 51 samples were positive by either PCR ELISA or culture; of these, 43 were found to be positive by PCR ELISA and 43 were found to be positive by culture. Overall, including positive and negative results, 59 samples were concordant in both methods. Several samples were positive in the PCR ELISA assay but were culture negative; therefore, this assay may be able to detect sublethally damaged or viable nonculturable forms of campylobacters. The method is rapid and sensitive, and it significantly reduces the time needed for the detection of these important pathogens by 2 to 3 days.

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NON-ANTIBODY COMPONENTS IN HUMAN MILK INHIBIT ESCHERICHIA COLI HEAT LABILE ENTEROTOXIN MEASURED BY AN ENZYME-LINKED IMMUNOSORBENT ASSAY

Acta Pathologica Microbiologica Scandinavica Section C Immunology ◽

10.1111/j.1699-0463.1980.tb00102.x ◽

2009 ◽

Vol 88C (1-6) ◽

pp. 247-254

Author(s):

ANNE-BRIT OTNAESS ◽

SVERRE HALVORSEN

Keyword(s):

Escherichia Coli ◽

Human Milk ◽

Immunosorbent Assay ◽

Enzyme Linked Immunosorbent Assay ◽

Heat Labile

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Rabbit antibodies against the low molecular weight folate binding protein from human milk. Use for immunological characterization of human folate binding proteins in an enzyme-linked immunosorbent assay (ELISA)

Bioscience Reports ◽

10.1007/bf01119771 ◽

1987 ◽

Vol 7 (7) ◽

pp. 553-557 ◽

Cited By ~ 19

Author(s):

Mimi Høier-Madsen ◽

Steen Ingemann Hansen ◽

Jan Holm

Keyword(s):

Molecular Weight ◽

Human Milk ◽

Immunosorbent Assay ◽

Binding Proteins ◽

Cross Reactivity ◽

Enzyme Linked Immunosorbent Assay ◽

Low Molecular Weight ◽

Folate Binding Protein ◽

Folate Binding Proteins

Antibodies to a low molecular weight folate binding protein isolated from human milk were raised in rabbits and used for development of a two-site enzyme-linked immunosorbent assay (ELISA) for immunological characterization of human folate binding proteins (FBPs). The high and low molecular weight FBPs from human milk were immunologically indistinguishable. Furthermore, the FBPs in human urine and cerebrospinal fluid showed a cross-reactivity of 70% and 30%, respectively. No cross-reactivity of the FBP from cow's milk was observed.

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