scholarly journals Combining microsatellites, growth, and adaptive traits for managing in situ genetic resources of Eucalyptus urophylla

2007 ◽  
Vol 37 (4) ◽  
pp. 773-785 ◽  
Author(s):  
Vincent Tripiana ◽  
Michaël Bourgeois ◽  
Daniel Verhaegen ◽  
Philippe Vigneron ◽  
Jean-Marc Bouvet

Genetic diversity and structure of 17 populations representative of the natural range of Eucalyptus urophylla S.T. Blake in Indonesia were analysed with 10 microsatellite markers. Two provenance and progeny trials, using the same populations, were established in the Republic of the Congo and analysed for growth and survival at 37 months. Observed microsatellite heterozygosity (Ho) was moderate to high within populations (Ho = 0.51–0.72). The index of fixation (FIS) was significantly different from zero for all populations (FIS = 0.13–0.31) and possibly resulted from a Wahlund effect. The differentiation parameter was low (FST = 0.04) and not significantly different from zero, which can be explained by effective gene flow via pollen. The genetic variances within and among provenances for survival and growth traits were significantly different from zero, representing 13%–23% and 14%–50% of the total variation, respectively. The differentiation between populations based on quantitative traits was marked (QST = 0.07–0.33). A negative relationship between altitude of the seed source and its performance in the Congo was observed (R2 = 0.59–0.67) and could be explained by the effect of natural selection along the altitudinal gradient. This marked differentiation for quantitative traits despite high apparent gene flow results in a clinal variation, which suggests the use of altitude of seed source as an important principle for in situ or ex situ management of E. urophylla genetic resources.

Author(s):  
Roland Bourdeix ◽  
Steve Adkins ◽  
Vincent Johnson ◽  
Lalith Perera ◽  
Sisunandar

Genetika ◽  
2004 ◽  
Vol 36 (3) ◽  
pp. 221-227
Author(s):  
Jelena Aleksic ◽  
Sasa Orlovic

Principles of the conservation of genetic resources of elms (Ulmus spp) do not differ fundamentally from the general principles accepted for the conservation of genetic resources of other common Noble Hardwoods. Efficient conservation can best be achieved through appropriate combination of in situ and ex situ methods, which have distinct advantages. Besides that, ex situ conservation is employed when emergency measures are needed for rare endangered populations and when populations are too small to be managed in situ (e.g. risks of genetic drift and inbreeding). The aim of our research is ex situ conservation of genetic resources of field elm {Ulmus minor Mill) and European white elm (Ulmus laevis Pall) through establishment of field genebanks. Sampling was conducted in one population of field elm and one population of white elm. Plant material (buds) from 8 trees of field elm and 10 trees of white elm was used for in vitro production of clones. Obtained clones will be used for establishment of field genebanks on the experimental estate of the Institute of Lowland Forestry and Environment.


2020 ◽  
Vol 17 (2) ◽  
pp. 31-42
Author(s):  
MA Hamid

The present article addresses the scenario of duck genetic resources, their production and reproduction performances, their improvement and conservation in Bangladesh. The duck, among other poultry species available in the country, are used for meat and egg production. The duck germplasm available in the country are indigenous/native, improved native, exotic and their crosses. The native duck population is comprised of Indigenous Non-descript, Deshi White, Deshi Black, Nageshwari, Sylhet Mete etc. The improved native such as, BLRI-1 and BLRI-2 are developed by Bangladesh Livestock Research Institute. The exotic duck such as, Khaki Campbell, Indian Runner, Jending, Muscovy, White Pekin, Cherry Valley, Thailand Black etc. are also used at farms level. The crossbred of different ducks are used in the country at different farming systems. Improvement and conservation of native duck are ongoing both in-situ and ex-situ in vivo by DLS, BLRI, BAU and also by others. The present study provided baseline information on duck germplasm of Bangladesh which could be useful for future genetic characterization, improvement and conservation. SAARC J. Agri., 17(2): 31-42 (2019)


Author(s):  
Jane Muthoni ◽  
Hussein Shimelis ◽  
Rob Melis

Plant genetic resources (PGRs) play an important role in agriculture, environment protection, cultural property and trade; they need to be conserved. There are two fundamental approaches for the conservation of PGRs: in situ and ex situ. In situ conservation is the conservation of ecosystems and natural habitats and the maintenance and recovery of viable populations of species in their natural surroundings. Ex situ preservation is the storage of seeds or plant materials under artificial conditions to maintain their long term viability and availability for use. Genebanks employ seed storage, field collections of living plants and in vitro storage (tissue culture or cryopreservation) for ex situ preservation of PGR. Storage of orthodox seeds, which are tolerant to low moisture content and low temperatures at appropriate temperature and humidity, is the most convenient ex situ conservation method. Plants that produce recalcitrant seeds or non-viable seeds are conserved in field genebanks as well as in-vitro in slow growth media for short-to-medium term and cryopreservation in liquid nitrogen at -1960C for long-term periods. Cryopreservation is very expensive and needs trained personnel; this could explain why this method is rarely used for conservation of plant genetic resources in most developing countries. Potato tubers are bulky and highly perishable; the crop is generally conserved as clones either in field genebanks (with annual replanting), in-vitro conservation in slow growth media for short-to-medium term and cryopreservation for long term. Field genebanks are expensive to maintain and the crop is exposed to many dangers; hence, cryopreservation is the only feasible method for long term conservation. However, given the high cost of cryopreservation, long-term conservation of potato genetic resources is poorly developed in most resource-poor countries leading to high rates of genetic erosion. This paper looks into the various methods that that can be applied to conserve potato genetic resources and the status of conservation of potatoes in major genebanks and some countries.


2007 ◽  
Vol 41 ◽  
pp. 65-74 ◽  
Author(s):  
S.J. Hiemstra ◽  
A.G. Drucker ◽  
M.W. Tvedt ◽  
N. Louwaars ◽  
J.K. Oldenbroek ◽  
...  

SummaryThis paper addresses major issues and challenges for Animal Genetic Resources (AnGR) and the livestock sector, as well as options for further development of policies or regulatory approaches. Three main areas were identified, i) how we can halt the further erosion of genetic diversity and promote sustainable breeding and use, ii) whether there is a need to regulate the exchange of genetic material and iii) how to balance different systems of rights (e.g. sovereign rights of nations, intellectual property rights, communal rights or rights of livestock keepers).To halt further erosion, complementary ex-situ and in-situ conservation approaches are needed and breeding and marketing of local breeds should be strengthened. Secondly, recognizing the importance of the exchange of AnGR, broad access and responsible and equitable exchange mechanisms should be further promoted. Thirdly, regarding intellectual property rights, there is a need to adapt the application of the patent system to the special circumstances inherent in animal breeding. Moreover, possible sui generis systems should be further explored in order to better balance different rights systems.Rather than developing a new or adapted internationally legally binding framework, the intergovernmental process under FAO may instead wish to focus, in the first instance, on the development of voluntary instruments to strengthen national policies and the implementation of action at national levels.Debates and developments related to international agreements in the crop sector have also tended to frame the debate for AnGR. However, before launching into a discussion on whether or not an ‘FAO Animal Treaty’ would be needed, one should first of all clarify the problems to be dealt with and regulated via an international regime.


2016 ◽  
Vol 52 ◽  
pp. 211-220 ◽  
Author(s):  
O. V. Kruglyak ◽  
I. S. Martynyuk

The aim was to determine the amount of state financial support for the full gene pool preservation of local and endangered breeds of farm animals in Ukraine for 2016-2020. Given the great strategic and economic importance of the problem of gene pool preservation and rational use to enhance food security, the level of state financial support for preservation of the gene pool of local and endangered breeds of farm animals was determined for using two methods of conservation: in situ (live animals) and ex situ (cryoconservation of their genetic materials). The level of budget support to preserve gene pool objects involves calculating the minimum (normative) amount of genetic resources (livestock females, bull semen, embryos) and rational choice of methodical approach to determining the level of budget support for the preservation of each type of genetic resources. The level of budget support for in situ maintenance of gene pool objects was determined based on level of budget subsidy per head and livestock number of each species, recommended to preserve (normative). The basis of the level of budget subsidies was determined as compensation amount of normative costs for feed produced at cost. Normative costs of feed were determined on the basis of scientifically grounded rations for species and breeds of animals taking into account their productivity. The full in situ preservation of dairy and dual-purpose breeds of cattle requires the holding herds of each breed which include animals of all main bloodlines, where at least 3 live bulls and 60 cows should be kept per bloodline. In gene pool subjects of beef breeds at least 3 bulls and 20 cows should be kept per bloodline. Breeding cattle of all breeds should be performed by linear purebred breeding and randomized fixing of bulls in lines. Under these conditions, it is possible to preserve purebred livestock number for 5-6 generations, or 20 years. To preserve in situ the gene pool of local and endangered breeds of pigs, sheep and poultry it is necessary to provide herd size not less than 25 boars and 100 sows, 20 rams and 200 ewes, 10 stallions and 50 mares, 50 geese and 200 fowl, 50 drakes and 250 ducks, 50 cocks and 250 hens. The constancy of gene pool of local and endangered breeds is fully possible to provide only by ex situ method, providing measures to preserve genetic fund of breeds, types, lines in an artificial environment (cryoconservation of gametes, somatic cells, zygotes, tissues). Ex situ preservation of the gene pool of local and endangered breeds is expedient as a "virtual" cryo-herds. For their expansion annually 1-3 thousand doses of bull semen of dairy, dual-purpose and beef breeds per each farm or genealogical line (depending on the number of bulls in a line) must be collected and stored in cryo-depositories in over 3 thousand doses of bull semen for each line. Annual accumulation and storage of semen of rams should be accordingly 1.2 and 2.4 thousand doses, boars – 2 thousand doses and stallions – 300 doses in both cases. The annual need for purchasing sires’ semen of farm animals is 35.7 thousand doses. For implementation of programs for preserving the gene pool of local breeds it is necessary to pass 10% of annual purchased semen to the Bank of Animal Genetic Resources of Institute of Animal Breeding and Genetics nd. a. M.V.Zubets of NAAS. The normative costs for an annual storage of sires’ semen in 2015 have been determined, which amount is UAH 4.54 and UAH 2.98 according to normative capacity of cryo-depositories (5 thousand doses and 20 thousand doses). The project of preserving populations of local and endangered breeds of cattle as "cryo-herds" (cryopreserved bovine embryos) during 2016-2020 requires the state support for obtaining 250 bovine embryos, prepared for long-term storage, of each breed and storing them in Bank of Animal Genetic Resources of Institute of Animal Breeding and Genetics nd. a. M.V.Zubets of NAAS. To provide the full preservation of the gene pool of local and endangered breeds of farm animals in Ukraine using two methods of conservation: in situ (live animals) and ex situ (“virtual cryo-herds”) during the next five years it is necessary to finance UAH 75,137.5 thousand from the State Budget. The implementation of the developed economic mechanism ensures the gene pool preservation of domestic autochthonous and local breeds of farm animals and their further use for breeding animals of specialized and dual-purpose breeds, the execution of commitments laid down by international agreements of Ukraine for the biodiversity preservation. These research results have been used in the development of the Programme of preservation of local and endangered breeds of farm animals in Ukraine for 2016-2020.


2018 ◽  
pp. 79-106
Author(s):  
Sheel Yadav ◽  
Chet Ram ◽  
DhammaPrakash Pandhari Wankhede

1996 ◽  
Vol 72 (4) ◽  
pp. 406-415 ◽  
Author(s):  
Alvin D. Yanchuk ◽  
Donald T. Lester

Gene conservation of native conifer species in British Columbia is considered necessary primarily to safeguard the future evolutionary potential of species to climate change, new biotic challenges and for commercial genetic improvement programs. The tactical options include: (i) maintaining existing protected areas, (ii) creating new reserves for in situ management, and (iii) ex situ collections of various types.A two-part strategy is presented, with emphasis on technical justifications, for the conservation of conifer tree genetic resources of 23 British Columbia species. Part I is a survey of the frequency of each species in current land reserves in BC. Part II outlines an approach that will aid in setting priorities for additional gene conservation activities for specific species. This process attempts to use information on: (i) levels of in situ protection, (ii) the status of each species in current provenance research and breeding programs, and (iii) the relative capabilities for natural regeneration for each species.Representation of the 23 native conifer species in the current network of protected areas is generally complete. For instance, western hemlock is under little threat, as extensive ex situ collections are in field tests; it is well protected in the current reserve network, and it has a great capacity for natural regeneration. At the other extreme, whitebark pine has no ex situ collections made to date, needs additional protection in some ecoregions of the province, and generally has poor natural regeneration potential. While implementation of our strategy will be an ongoing process (i.e. updating information from both new and old in situ reserves, and setting new priorities among species), several immediate issues have been noted. These include; (i) follow-up work in the area of population sampling for ex situ collections, (ii) "ground truthing" of current reserves where data are weak, and (iii) examining the merit of certain populations not located in BC (as they could be as important as those currently protected in BC).For the future, various broad management questions will need to be resolved, such as: (i) whether existing reserves will perpetuate the various gene pools, and what management will be appropriate for such areas, (ii) the impact of pollen migration from genetically improved production stands on reserve stands, and (iii) the role of commercially improved stands and the breeding populations themselves to meet gene conservation objectives. Key words: gene conservation, native conifers, British Columbia, tree genetics


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