Clonal testing efficiency: the trade-offs between clones tested and ramets per clone

1986 ◽  
Vol 16 (5) ◽  
pp. 925-930 ◽  
Author(s):  
J. H. Russell ◽  
W. J. Libby

Three contrasting simulation models were developed to investigate testing efficiencies in a clonal selection program. The variables investigated were number of total plants tested, number of candidate clones tested, number of ramets per clone, number of clones selected, selection intensity, and broad-sense heritability. The model deemed appropriate to most clonal forestry situations selected a fixed number of clones in an experiment with the total number of plants in the test held constant. In this model, as the number of ramets per clone was varied, the number of candidate clones tested and the selection intensity necessarily also varied. This model indicates that cloning individuals for testing is useful when selection is based on a characteristic or index with broad-sense heritability less than about 0.6. At the lower heritabilities, two to six ramets per clone per site usually produces the optimum level of cloning, the exact number depending upon the selection intensity and heritability. Predictions generated by this fixed number of selected clones model were compared with average phenotypic values of selections using different subsamples of data for 8-year height and for 8-year diameter in a radiata pine (Pinusradiata D. Don) clonal experiment. Agreement between predictions and average phenotypic values in both these two comparisons was close.

2006 ◽  
Vol 36 (9) ◽  
pp. 2148-2156 ◽  
Author(s):  
Salvador A Gezan ◽  
Timothy L White ◽  
Dudley A Huber

Clonal testing was studied under different environmental patterns and experimental designs through simulation with the criteria of maximizing broad-sense heritability estimates and genetic gain from clonal selection. Several experimental designs were studied together with three patterns of environmental variability. In addition, empirical 95% confidence intervals for heritability estimates were compared with Dickerson's approximate method. Other elements studied included (i) conditions under which different environmental patterns yield high or low heritabilities and (ii) effects of varying the number of ramets per clone. Row-column designs produced the highest mean individual broad- sense heritability, but these designs were only slightly more efficient than incomplete block designs with small block sizes. For all experimental designs, Dickerson's approximate method for estimating the variance of heritability estimates produced reasonable 95% confidence intervals but overestimated the upper confidence limit of complex designs. Larger heritabilities were found with higher tree-to-tree spatial correlations and lower amounts of microsite residual variance, and varying gradients had negligible influence. The effect of implementing Latinization was significant on increasing heritability, but small in practical terms, and was more important for patchy surfaces. Experiments with more ramets per clone yielded higher clonal mean heritabilities, and using between four and six ramets per clone per site is recommended.


2021 ◽  
Author(s):  
Ghasem Eghlima ◽  
Mohsen Sanikhani ◽  
Azizollah Kheiry ◽  
Javad Hadian

Abstract Glycyrrhiza glabra L. is an herbaceous, perennial plant with high distribution in Iran. Genetic variability, heritability and correlation among characters in 22 populations of G. glabra L. were studied. The genetic parameters among the traits including phenotypic variances, genotypic variances, genotype by environment variances, broad-sense heritability and genotypic and phenotypic correlation coefficients were studied. Variance components analysis showed that the extent of phenotypic coefficient of variation (PCV) was fairly higher for all the examined traits compared with genotypic coefficient of variation (GCV). Glabridin (GLA) exhibited high GCV and PCV (156.07% and 156.68%, respectively). The broad sense heritability varied from 38.92–99.79% and narrow sense heritability ranged from 9.70 % to 24.94%. Heritability of GLA, glycyrrhizic acid (GLY), liquiritin (LI), liquiritigenin (LIQ), rutin (RU) and rosmarinic acid (RA) were very high, exhibiting more than 97% heritability. Therefore, these critical characteristics can efficiently be selected and inherited in breeding programs. In most traits, the genotypic correlations showed the same direction as the phenotypic correlations. The contents of GLA and LIQ showed a positive correlation with majority of morphological traits. Therefore, selecting individual plants having desired morphological traits can be correlated with high contents of bioactive compounds in the harvested root.


2004 ◽  
Vol 34 (2) ◽  
pp. 505-510 ◽  
Author(s):  
Marcelo Renato Alves de Araújo ◽  
Bruce Coulman

Meadow bromegrass (Bromus riparius Rehm.) is a recently introduced pasture grass in western Canada. Its leafy production and rapid regrowth have made it a major grass species for pasturing beef animals in this region. As relatively little breeding work has been done on this species, there is little information on its breeding behaviour. The main objective of this study was to estimate total genetic variability, broad-sense heritability, phenotypic and genetic correlations. Forty-four meadow bromegrass clones were evaluated for agronomic characters. Genetic variation for dry matter yield, seed yield, fertility index, harvest index, plant height, plant spread, crude protein, neutral detergent fiber and acid detergent fiber, was significant. Broad-sense heritability estimates exceeded 50% for all characters. Heritability estimates were at least 3.5 times greater than their standard errors. Phenotypic and genetic correlation between all possible characters were measured. There was general agreement in both sign and magnitude between genetic and phenotypic correlations. Correlations between the different characters demonstrated that it is possible to simultaneously improve seed and forage yield. Based on the results, it appears that the development of higher yielding cultivars with higher crude protein, and lower acid and neutral detergent fibers concentration should be possible.


1988 ◽  
Vol 18 (8) ◽  
pp. 1059-1062 ◽  
Author(s):  
Robert E. Farmer Jr. ◽  
Karen Garlick ◽  
Steven R. Watson

A 20-clone population of Populusbalsamifera L. was grown for 3 years in a field test which allowed evaluation of variance due to clones and to primary ramets within clones, the source of cuttings. Primary ramets did not influence rate of summer shoot elongation, which had a broad-sense heritability of 0.24 and 0.16 in the 2nd and 3rd years, respectively. By the end of the 2nd year, height variation associated with primary ramets (i.e., C effects) was nonsignificant, and broad-sense heritability for height was about 0.50.


2002 ◽  
Vol 32 (11) ◽  
pp. 1961-1969 ◽  
Author(s):  
T H Jones ◽  
B M Potts ◽  
R E Vaillancourt ◽  
N W Davies

This study investigated the association between resistance of Eucalyptus globulus Labill. to autumn gum moth (Mnesempala privata Guenée) defoliation and cuticular wax compounds. In a field trial consisting of clonally replicated F2 families of E. globulus, situated in Tasmania, Australia, significant genetic variation in resistance was detected in two of three F2 families. The broad-sense heritability for defoliation within families ranged from 0.24 to 0.33. The 15 most resistant and the 15 most susceptible genotypes within each variable family were compared for their relative levels of 26 cuticular wax compounds. While no significant correlation between resistance and total wax yield estimates was found, significant differences were detected between resistant and susceptible classes in the relative quantities of several aliphatic phenylethyl and benzyl wax esters within both families. This association does not appear to be a response induced by defoliation. The broad-sense heritabilities of the variation in these compounds were high (0.82–0.94). Our findings suggest that these wax compounds are a mechanism of genetic resistance to autumn gum moth in E. globulus.


2021 ◽  
Author(s):  
John Stover ◽  
Sherrie L. Kelly ◽  
Edinah Mudimu ◽  
Dylan Green ◽  
Tyler Smith ◽  
...  

AbstractIntroductionThe COVID-19 pandemic has caused widespread disruptions including to health services. In the early response to the pandemic many countries restricted population movements and some health services were suspended or limited. In late 2020 and early 2021 some countries re-imposed restrictions. Health authorities need to balance the potential harms of additional SARS-CoV-2 transmission due to contacts associated with health services against the benefits of those services, including fewer new HIV infections and deaths. This paper examines these trade-offs for select HIV services.MethodsWe used four HIV simulation models (Goals, HIV Synthesis, Optima HIV and EMOD) to estimate the benefits of continuing HIV services in terms of fewer new HIV infections and deaths. We used three COVID-19 transmission models (Covasim, Cooper/Smith and a simple contact model) to estimate the additional deaths due to SARS-CoV-2 transmission among health workers and clients. We examined four HIV services: voluntary medical male circumcision, HIV diagnostic testing, viral load testing and programs to prevent mother-to-child transmission. We compared COVID-19 deaths in 2020 and 2021 with HIV deaths occurring now and over the next 50 years discounted to present value. The models were applied to countries with a range of HIV and COVID-19 epidemics.ResultsMaintaining these HIV services could lead to additional COVID-19 deaths of 0.002 to 0.15 per 10,000 clients. HIV-related deaths averted are estimated to be much larger, 19 - 146 discounted deaths per 10,000 clients.DiscussionWhile there is some additional short-term risk of SARS-CoV-2 transmission associated with providing HIV services, the risk of additional COVID-19 deaths is at least 100 times less than the HIV deaths averted by those services. Ministries of Health need to take into account many factors in deciding when and how to offer essential health services during the COVID-19 pandemic. This work shows that the benefits of continuing key HIV services are far larger than the risks of additional SARS-CoV-2 transmission.


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