PREPARATION AND PROPERTIES OF TRANSKETOLASE FROM PORK LIVER

1960 ◽  
Vol 38 (1) ◽  
pp. 115-124 ◽  
Author(s):  
F. J. Simpson
Keyword(s):  
Heavy Metal ◽  
Metal Ions ◽  
Heavy Metal Ions ◽  
Thiamine Pyrophosphate ◽  
Magnesium Ions ◽  
Optimum Activity ◽  
Pork Liver ◽  
High Concentrations ◽  
The Stability ◽  
Ph Range

Transketolase of pork liver has been purified 90-fold and separated from ribulose 5-phosphate 3-epimerase. The transketolase is most stable between pH 7.5 and 8.5 and below 40 °C. The pH range for optimum activity is between 7.6 and 8.1. Activation by magnesium ions or thiamine pyrophosphate could not be demonstrated, but thiamine pyrophosphate increased the stability of the enzyme. Sulphydryl agents, such as p-chloromercuriphenyl sulphonic acid and N-ethylmaleimide, and heavy metal ions, such as cupric, mercuric, and zinc, at relatively high concentrations inhibited the enzyme.

PREPARATION AND PROPERTIES OF TRANSKETOLASE FROM PORK LIVER

1960 ◽  
Vol 38 (2) ◽  
pp. 115-124 ◽  
Author(s):  
F. J. Simpson
Keyword(s):  
Heavy Metal ◽  
Metal Ions ◽  
Heavy Metal Ions ◽  
Thiamine Pyrophosphate ◽  
Magnesium Ions ◽  
Optimum Activity ◽  
Pork Liver ◽  
High Concentrations ◽  
The Stability ◽  
Ph Range

Transketolase of pork liver has been purified 90-fold and separated from ribulose 5-phosphate 3-epimerase. The transketolase is most stable between pH 7.5 and 8.5 and below 40 °C. The pH range for optimum activity is between 7.6 and 8.1. Activation by magnesium ions or thiamine pyrophosphate could not be demonstrated, but thiamine pyrophosphate increased the stability of the enzyme. Sulphydryl agents, such as p-chloromercuriphenyl sulphonic acid and N-ethylmaleimide, and heavy metal ions, such as cupric, mercuric, and zinc, at relatively high concentrations inhibited the enzyme.

Facile one-pot synthesis of highly monodisperse nickel microspheres with raised nickel dots and their adsorption performance for heavy metal ions

2016 ◽  
Vol 45 (23) ◽  
pp. 9704-9711 ◽  
Author(s):  
Yue Teng ◽  
Le Xin Song ◽  
Wei Liu ◽  
Li Zhao ◽  
Juan Xia ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Metal Ions ◽  
Solvothermal Synthesis ◽  
Heavy Metal Ions ◽  
One Pot ◽  
Adsorption Performance ◽  
One Pot Synthesis ◽  
High Concentrations

Monodispersed Ni microspheres with surfaces uniformly covered by Ni dots were constructed through a facile solvothermal synthesis, showing an improved ability to remove Cd2+ ions even at high concentrations in water.

scholarly journals Comparative study of binding strengths of heavy metals with humic acid

2013 ◽  
Vol 67 (5) ◽  
pp. 773-779 ◽  
Author(s):  
Ivana Kostic ◽  
Tatjana Andjelkovic ◽  
Ruzica Nikolic ◽  
Tatjana Cvetkovic ◽  
Dusica Pavlovic ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Humic Acid ◽  
Metal Ions ◽  
Stability Constants ◽  
Metal Ion ◽  
Heavy Metal Ions ◽  
Exchange Method ◽  
Divalent Metal Ion ◽  
The Stability

The complexation of humic acid with certain heavy metal ions (Co(II), Ni(II), Cu(II), Zn(II) and Pb(II)) was investigated. The stability constants of humate complexes were determined by method which is based on distribution of metal ions between solution and resin in the presence and the absence of ligand, known as Schubert?s ion exchange method. Experiments were performed at 25 ?C, at pH 4.0 and ionic strength of 0.01 mol dm-3. It was found that the 1:1 complexes were formed between metal ions and humic acid. Obtained results of the stability constants, log ?mn, of complexes formed between the metal ions and humic acid follow the order Co(II) < Ni(II) < Cu(II) > Zn(II) which is the same like in the Irving-Williams series for the binding strength of divalent metal ion complexes. Stability constant of complex between Pb(II) ions and humic acid is greater than stability constants of other investigated metal-humate complexes. The investigation of interaction between heavy metal ions and humics is important for the prediction of the distribution and control of the migration of heavy metals in natural environment.

scholarly journals Secondary pollution of water during the biosorption of heavy metal ions by pristine and sugar beet shreds from bioethanol production

2018 ◽  
pp. 81-91 ◽  
Author(s):  
Dragana Kukic ◽  
Marina Sciban ◽  
Vesna Vasic ◽  
Jelena Prodanovic
Keyword(s):  
Heavy Metal ◽  
Sugar Beet ◽  
Metal Ions ◽  
Adsorption Capacity ◽  
Heavy Metal Ions ◽  
Bioethanol Production ◽  
Limit Values ◽  
Secondary Pollution ◽  
Emission Limit ◽  
High Concentrations

When biomaterials are used as adsorbents, some compounds present in their structure can be extracted and cause secondary pollution of the treated water. Depending on the type and amount of the secondary pollution, some materials can be unacceptable as adsorbents despite of their good adsorption capacity. Although, it is not a newly discovered phenomenon and it is recognized as a problem, leaching from adsorbents is neglected. The aim of our study is to investigate the secondary pollution of the water during the biosorption of heavy metal ions by pristine and modified sugar beet shreds from bioethanol production. The presence of different elements in the mentioned adsorbents, secondary pollution of the water during the adsorption of heavy metal ions and amount of reducing sugars in the water treated by pristine sugar beet shreds were characterized by the SEM-EDS method, the amount of the leached inorganic and organic matter from the adsorbents (atomic absorption spectroscopy and permanganate index) and Bertrand's method, respectively. Furthermore, the obtained results were compared to the available literature data. Our results showed that according to the valid regulations of emission limit values of the polluting substances in the water and adsorption capacity, the investigated adsorbents have a great potential as biosorbents for practical treatment of wastewaters which contain high concentrations of heavy metal ions.

Determination of Trace Heavy Metal Ions in Water by FAAS after Preconcentration with ASTO

2013 ◽  
Vol 826 ◽  
pp. 267-270
Author(s):  
En Jun Song
Keyword(s):  
Heavy Metal ◽  
Metal Ions ◽  
Heavy Metal Ions ◽  
Absorption Spectrometry ◽  
Environmental Water ◽  
Flame Atomic Absorption ◽  
Experimental Parameters ◽  
Ph Range ◽  
Coexisting Ions

A new method of preconcentration with nanometer-strontium titanate immobilized on aluminum oxide (ASTO) combined with flame atomic absorption spectrometry (FAAS) was proposed for the determination of trace heavy metal ions, included Cu2+, Ni2+ and Ag+, in environmental water. The optimum experimental parameters for preconcentration of the heavy metals studied, such as pH, shaking time, eluent and coexisting ions, have been investigated. The results showed that Cu2+, Ni2+ and Ag+, could be quantitatively retained by the ASTO in the pH range of 5-8, and then eluted completely with 2 mol·L-1 HNO3. The detection limits of this method for Cu2+, Ni2+ and Ag+ were 0.031μg·L-1, 0.15μg·L-1and0.041μg·L-1 with an enrichment factor of 50, respectively. The method has been applied to the simultaneous determination of trace amounts of Cu2+, Ni2+ and Ag+ in environmental water, the recoveries were all in 95%-105%.

scholarly journals Influence of transition and heavy metal ions on ATP-ases activity in rat synaptic plasma membranes

2004 ◽  
Vol 69 (7) ◽  
pp. 541-547 ◽  
Author(s):  
Ljubica Vujisic ◽  
Danijela Krstic ◽  
Katarina Krinulovic ◽  
Vesna Vasic
Keyword(s):  
Heavy Metal ◽  
Metal Ions ◽  
Heavy Metal Ions ◽  
Plasma Membranes ◽  
Model System ◽  
Half Maximum ◽  
Synaptic Plasma Membranes ◽  
Equilibrium Binding ◽  
Inhibitory Activities ◽  
The Stability

The influence of transition metal (Cu2+, Zn2+, Fe2+ and Co2+) and heavy metal ions (Hg2+, Pb2+ and Cd2+) on the activities of Na+/K+-ATPase and Mg2+-ATPase isolated from rat synaptic plasma membranes (SPM) was investigated. The aim of the study was to elucidate the inhibition of both ATPase activities by exposure to the considered metal ions as a function of their affinity to bind to the ?SH containing ligand L-cysteine, as a model system. The half-maximum inhibitory activities (IC 50) of the enzymes were determined as parameters of rectangular hyperbolas and correlated with the stability constant (K s) of the respectivemetal- ion-L-cysteine complex. The linear Dixon plots indicate equilibrium binding of the investigated ions to both enzymes.

scholarly journals Heavy metal complexation by surfaces and humic acids: a brief discourse on assessment by acidimetric titration.

1987 ◽  
Vol 35 (3) ◽  
pp. 231-240 ◽  
Author(s):  
Z.J. Wang ◽  
W. Stumm
Keyword(s):  
Heavy Metal ◽  
Metal Ions ◽  
Metal Ion ◽  
Heavy Metal Ions ◽  
Natural Waters ◽  
Ion Concentration ◽  
Natural Sample ◽  
Voltammetric Measurement ◽  
Ph Dependent ◽  
The Stability

In natural waters the form of occurrence of heavy metal ions needs to be known to understand the factors that control their concentrations, their reactivity and bioavailability. In natural samples, it is difficult to identify the species present and to distinguish between dissolved and particulate concentrations, because of the lack of sufficiently sensitive and specific analytical detectors. It is suggested that the voltammetric measurement of the labile metal ion concentration as a function of pH (from an acidimetric titration of a natural sample) may provide useful information on the pH-dependent distribution of soluble and particulate concentrations and on the stability of (surface and solute) complexes formed. (Abstract retrieved from CAB Abstracts by CABI’s permission)

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